United States
Germany
Japan
United Kingdom
China
EIF3S2 (EIF3I) (NM_003757) Human Untagged Clone
CAT＃: SC117777
EIF3I (untagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I)
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | eIF3-beta; eIF3-p36; EIF3S2; PRO2242; TRIP-1; TRIP1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117777 sequence for NM_003757 edited (data generated by NextGen Sequencing)
ATGAAGCCGATCCTACTGCAGGGCCATGAGCGGTCCATTACGCAGATTAAGTATAACCGC GAAGGAGACCTCCTCTTTACTGTGGCCAAGGACCCTATCGTCAATGTATGGTACTCTGTG AATGGTGAGAGGCTGGGCACCTACATGGGCCATACCGGAGCTGTGTGGTGTGTGGACGCT GACTGGGACACCAAGCATGTCCTCACTGGCTCAGCTGACAACAGCTGTCGTCTCTGGGAC TGTGAAACAGGAAAGCAGCTGGCCCTTCTCAAGACCAATTCGGCTGTCCGGACCTGCGGT TTTGACTTTGGGGGCAACATCATCATGTTCTCCACGGACAAGCAGATGGGCTACCAGTGC TTTGTGAGCTTTTTTGACCTGCGGGATCCGAGCCAGATTGACAACAATGAGCCCTACATG AAGATCCCTTGCAATGACTCTAAAATCACCAGTGCTGTTTGGGGACCCCTGGGGGAGTGC ATCATCGCTGGCCATGAGAGTGGAGAGCTCAACCAGTATAGTGCCAAGTCTGGAGAGGTG TTGGTGAATGTTAAGGAGCACTCCCGGCAGATCAACGACATCCAGTTATCCAGGGACATG ACCATGTTTGTGACCGCGTCCAAGGACAACACAGCCAAGCTTTTTGACTCCACAACTCTT GAACATCAGAAGACTTTCCGGACAGAACGTCCTGTCAACTCAGCTGCCCTCTCCCCCAAC TATGACCATGTGGTCCTGGGCGGTGGTCAGGAAGCCATGGATGTAACCACAACCTCCACC AGGATTGGCAAGTTTGAGGCCAGGTTCTTCCATTTGGCCTTTGAAGAAGAGTTTGGAAGA GTCAAGGGTCACTTTGGACCTATCAACAGTGTTGCCTTCCATCCTGATGGCAAGAGCTAC AGCAGCGGCGGCGAAGATGGTTACGTCCGTATCCATTACTTCGACCCACAGTACTTCGAA TTTGAGTTTGAGGCTTAA Clone variation with respect to NM_003757.2 >OriGene 5' read for NM_003757 unedited
TCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCACGTTGCGGCCTTCCTCGCGTCAC AGCCGGGATGAAGCCGATCCTACTGCAGGGCCATGAGCGGTCCATTACGCAGATTAAGTA TAACCGCGAAGGAGACCTCCTCTTTACTGTGGCCAAGGACCCTATCGTCAATGTATGGTA CTCTGTGAATGGTGAGAGGCTGGGCACCTACATGGGCCATACCGGAGCTGTGTGGTGTGT GGACGCTGACTGGGACACCAAGCATGTCCTCACTGGCTCAGCTGACAACAGCTGTCGTCT CTGGGACTGTGAAACAGGAAAGCAGCTGGCCCTTCTCAAGACCAATTCGGCTGTCCGGAC CTGCGGTTTTGACTTTGGGGGCAACATCATCATGTTCTCCACGGACAAGCAGATGGGCTA CCAGTGCTTTGTGAGCTTTTTTGACCTGCGGGATCCGAGCCAGATTGACAACAATGAGCC CTACATGAAGATCCCTTGNCATGACTCTAANATCACCAGTGCTGTTTGGGGACCCCTGGG GGAGTGCATCATCGCTGGCCATGAGAGTGGAGAGCTCAACCAGTATAGTGCCAAGTCTGG AGAGGTGTTGTGAATGTTAAGAGCACTCCCGCAGATCACGACACCAGTATCCAGGACATG ACATGTTGTGACCGCGTCAAGACACACAGCCAGCTTTTTGACNCACAACTCTTGACATAN AAGACTTCCGGACAGACGTTCTGTCACTAGCTGCCCTCTCCCCAACTATGACATGTGTCC TGGGCCGTGGTCNGAANCCATGGATGTACCACCACCTCCCCAGATTGCCAGTTGAAGGCA GTTCTCCTTTTGCCTTGAAAAAAGTTGAAAATAAGGTCCTTTGGACTATCAAGGGTGCCT CCTCTGAGCAAACTCACACGCGGAAAATGTACNTCGATCATATTGACCAGACTTGATGT >OriGene 3' read for NM_003757 unedited
NNGGTAACTCTTGAACCGCGCCGCAATCTANGATCGATTTTTTTTTTTTTTTTTTGAGAC GGAGTTTTGCTCTTGTTGCTCAGGGTGGAGTGCAATGACGTGATCTCAGCTCACTGCAAC CTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTATA GGCGTGTGCCACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGAGGTTTCTCCAT GTTGGTCAGGCTGGTCTTAAACTCCTGACCTCAGGTGATCCGCCTGCCTTAAAAAAAAAG TGGTGGGATTACAGGCATGAGCCACCACGCCCGGCAGGAGATCCAGCTTCTTAAGCCTCA AACTCAAATTCGAAGTACTGTGGGTCGAAGTAATGGATACGGACGTAACCATCTTCGCCG CCGCTGCTGTAGCTCTTGCCATCAGGATGGAAGGCAACACTGTTGATAGGTCCAAAGTGA CCCTTGACTCTTCCAAACTCTTCTTCAAAGGCCAAATGGAAGAACCTGGCCTCAAACTTG CCAATCCTGGTGGAGGTTGTGGTTACATCCATGGCTTCCTGACCACCGCCCAGGACCACA TGGTCATAGTTGGGGGAGAGGGCAGCTGAGTTGACAGGACGTTCTGTCCGGAAAGTCTTC TGATGTTCAAGAGTTGTGGAGTCAAAAAGCTTGCCTGTGTTGTCCTTGGACGCGGTCACA AACATGGGCATGTCCCTGGATAACTGGATGTCGTTGATCTGCCCGGAGTGCTCCCTACAT TCACCAACACCTCTTCAGACTTGGCACTATCCTGGTTGACCTCTCACCTCCATGGCCAAC GATGATGCACTCCCCCAGGGTCCCCAAAACCACTGTTGATTTTAAACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003757 |
| Insert Size | 1370 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003757.1, NP_003748.1 |
| RefSeq Size | 1402 bp |
| RefSeq ORF | 978 bp |
| Locus ID | 8668 |
| UniProt ID | Q13347 |
| Domains | WD40 |
| Gene Summary | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201833 | EIF3I (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I) |
CNY 2,400.00 |
|
| RC201833L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201833L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), mGFP tagged |
CNY 5,890.00 |
|
| RC201833L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201833L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), mGFP tagged |
CNY 5,890.00 |
|
| RG201833 | EIF3I (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit I (EIF3I) |
CNY 4,000.00 |
|
| SC320585 | EIF3I (untagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I) |
CNY 2,400.00 |
