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ALAD (NM_000031) Human Untagged Clone
CAT＃: SC117775
ALAD (untagged)-Human aminolevulinate dehydratase (ALAD)
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALADH; PBGS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_000031, the custom clone sequence may differ by one or more nucleotides
ATGCAGCCCCAGTCCGTTCTGCACAGCGGCTACTTCCACCCACTACTTCGGGCCTGGCAGACAGCCACCA CCACCCTCAATGCCTCCAACCTCATCTACCCCATCTTTGTCACGGATGTTCCTGATGACATACAGCCTAT CACCAGCCTCCCAGGAGTGGCCAGGTATGGTGTGAAGCGGCTGGAAGAGATGCTGAGGCCCTTGGTGGAA GAGGGCCTACGCTGTGTCTTGATCTTTGGCGTCCCCAGCAGAGTTCCCAAGGACGAGCGGGGTTCCGCAG CTGACTCCGAGGAGTCCCCAGCTATTGAGGCAATCCATCTGTTGAGGAAGACCTTCCCCAACCTCCTGGT GGCCTGTGATGTCTGCCTGTGTCCCTACACCTCCCATGGTCACTGCGGGCTCCTGAGTGAAAACGGAGCA TTCCGGGCTGAGGAGAGCCGCCAGCGGCTGGCTGAGGTGGCATTGGCGTATGCCAAGGCAGGATGTCAGG TGGTAGCCCCGTCGGACATGATGGATGGACGCGTGGAAGCCATCAAAGAGGCCCTGATGGCACATGGACT TGGCAACAGGGTATCGGTGATGAGCTACAGTGCCAAATTTGCTTCCTGTTTCTATGGCCCTTTCCGGGAT GCAGCTAAGTCAAGCCCAGCTTTTGGGGACCGCCGCTGCTACCAGCTGCCCCCTGGAGCACGAGGCCTGG CTCTCCGAGCTGTGGACCGGGATGTACGGGAAGGAGCTGACATGCTCATGGTGAAGCCGGGAATGCCCTA CCTGGACATCGTGCGGGAGGTAAAGGACAAGCACCCTGACCTCCCTCTCGCCGTGTACCACGTCTCTGGA GAGTTTGCCATGCTGTGGCATGGAGCCCAGGCCGGGGCATTTGATCTCAAGGCTGCCGTACTGGAGGCCA TGACTGCCTTCCGCAGAGCAGGTGCTGACATCATCATCACCTACTACACACCGCAGCTGCTGCAGTGGCT GAAGGAGGAATGA >OriGene 5' read for NM_000031 unedited
GTCAAAATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCAATGCCCCAG GAGCCCTCGGTTCCAACCAACTGATGCCCCTGTGCCCACTGGCCCACGCCATGCAGCCCC AGTCCGTTCTGCACAGCGGCTACTTCCACCCACTACTTCGGGCCTGGCAGACAGCCACCA CCACCCTCAATGCCTCCAACCTCATCTACCCCATCTTTGTCACGGATGTTCCTGATGACA TACAGCCTATCACCAGCCTCCCAGGAGTGGCCAGGTACGGTGTGAAGCGGCTGGAAGAGA TGCTGAGGCCCTTGGTGGAAGAGGGCCTACGCTGTGTCTTGATCTTTGGCGTCCCCAGCA GAGTTCCCAAGGACGAGCGGGGTTCCGCAGCTGACTCCGAGGAGTCCCCAGCTATTGAGG CAATCCATCTGTTGAGGAAGACCTTCCCCAACCTCCTGGTGGCCTGTGATGTCTGCCTGT GTCCCTACACCTCCCATGGTCACTGCGGGCTCCTGAGTGAAAACGGAGCATTCCGGGCTG AGGAGAGCCGCCAGCGGCTGGCTGAGGTGGCATTGGCGTATGCCAAGGCAGGATGTCAGG TGGTAGCCCCGTCGGACATGATGGATGGACGCGTGGAAGCCATCANAGAGGCCCTGATGG CACATGGACTTGGCAACAGGGTATCGGTGATGAGCTACAGTGCCAAATTTTGCTTCTGTT TCTATGGCCCTTTCCGGGATGCAGCTAAGTCAAGCCCAGCTTTTGGNGACCGNCGCTGCT ACCAGCTNGCCCCTGGAGCACGAAGCCTGGCTCTCCGAGCTGTGGACGGGNATGTACGGG AAGGAGCTGAATGCTCATGGTGAACCNGGNATGCCCTACCTGGACTCGTGCGGNAGGTAA AGACAAGACCCGGACTCCTTTTCGCGGTACACGTCTTGGAAATTTGCCGA >OriGene 3' read for NM_000031 unedited
NNNNNCCCCCCCTAGGAGATGGCACTTCCAGGCCCAGAAAAGCACTGGGGNAGGGTCACA GGNATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG AGTTTTTTCTTTTTTTTTTATTGTACTTCAAAAATTGAATTTTTAATCAAAATTCTTTAT AAATTTATTACAATATAGATTTTGTGATGCATACCTTTGCCCACTGCAGGCTAATGTGAG TGTTCTGAGCAAGTTTAAGGCAGGCTAGGCTAACCTATGATGTTTGGAAGGTTAGGTATA TTAAATACATTTTTGACTCACGATCATATTTTCAACTTATGATCACATTTTCAACTTAGG ACGGGTTTATCAGGATGCAGACCCATCACATAAATGGAGGGGTGTCCATAAGACATTGCT AGACCTATAAATTTGCTGTTGAATAATGGGAGGTGGTATGGCACAGTGGTTAGGGGCATA GGCCTTGGAGTTGGACTACATGGGTTCAAATCCCAGCTTGGCTGTTTTCTGTGCAGTTCT AATCCAGTTCTGCCACAAACTTGTTGAGTGGCCTTAAGTGACTCACATTAGCACTCTAGA TATAGCTTCCTTATGTGGAAAAGGCGCAAGCAAACTTTAGTATTCTCCCTCCACCCCGAC CATGCTTCTGTTCCTCATCCAGGCTGCCCCATCTTAGTAAATGGACCATCAACCACCGCG AAGTCAAGCCAAATTAATCCTTCACATCGGATCTACCTTCTAAACACATCCCCGGTCCCA TTTCCCTTTCTCCATCGCACTGTCCCCGGTCCGGTCCATGACAAGAAGAATTTTCAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000031 |
| Insert Size | 3700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000031.4, NP_000022.2 |
| RefSeq Size | 3151 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 210 |
| UniProt ID | P13716 |
| Domains | ALAD |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Porphyrin and chlorophyll metabolism |
| Gene Summary | The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (2) lacks a segment of the coding region and initiates translation from an alternate start codon, compared to variant 1. The resulting isoform (b) has a distinct N-terminus, compared to isoform a. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201545 | ALAD (Myc-DDK-tagged)-Human aminolevulinate dehydratase (ALAD) |
CNY 3,656.00 |
|
| RC201545L1 | Lenti ORF clone of Human aminolevulinate dehydratase (ALAD), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201545L2 | Lenti ORF clone of Human aminolevulinate dehydratase (ALAD), mGFP tagged |
CNY 5,890.00 |
|
| RC201545L3 | Lenti ORF clone of Human aminolevulinate dehydratase (ALAD), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201545L4 | Lenti ORF clone of Human aminolevulinate dehydratase (ALAD), mGFP tagged |
CNY 5,890.00 |
|
| RG201545 | ALAD (tGFP-tagged) - Human aminolevulinate dehydratase (ALAD) |
CNY 4,370.00 |
