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B4GALT4 (NM_003778) Human Untagged Clone
CAT＃: SC117759
B4GALT4 (untagged)-Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 (B4GALT4), transcript variant 2
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | B4Gal-T4; beta4Gal-T4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003778, the custom clone sequence may differ by one or more nucleotides
ATGGGCTTCAACCTGACTTTCCACCTTTCCTACAAATTCCGATTACTGTTGCTGTTGACTTTGTGCCTGA CAGTGGTTGGGTGGGCCACCAGTAACTACTTCGTGGGTGCCATTCAAGAGATTCCTAAAGCAAAGGAGTT CATGGCTAATTTCCATAAGACCCTCATTTTGGGGAAGGGAAAAACTCTGACTAATGAAGCATCCACGAAG AAGGTAGAACTTGACAACTGCCCTTCTGTGTCTCCTTACCTCAGAGGCCAGAGCAAGCTCATTTTCAAAC CAGATCTCACTTTGGAAGAGGTACAGGCAGAAAATCCCAAAGTGTCCAGAGGCCGGTATCGCCCTCAGGA ATGTAAAGCTTTACAGAGGGTCGCCATCCTCGTTCCCCACCGGAACAGAGAGAAACACCTGATGTACCTG CTGGAACATCTGCATCCCTTCCTGCAGAGGCAGCAGCTGGATTATGGCATCTACGTCATCCACCAGGCTG AAGGTAAAAAGTTTAATCGAGCCAAACTCTTGAATGTGGGCTATCTAGAAGCCCTCAAGGAAGAAAATTG GGACTGCTTTATATTCCACGATGTGGACCTGGTACCCGAGAATGACTTTAACCTTTACAAGTGTGAGGAG CATCCCAAGCATCTGGTGGTTGGCAGGAACAGCACTGGGTACAGGTTACGTTACAGTGGATATTTTGGGG GTGTTACTGCCCTAAGCAGAGAGCAGTTTTTCAAGGTGAATGGATTCTCTAACAACTACTGGGGATGGGG AGGCGAAGACGATGACCTCAGACTCAGGGTTGAGCTCCAAAGAATGAAAATTTCCCGGCCCCTGCCTGAA GTGGGTAAATATACAATGGTCTTCCACACTAGAGACAAAGGCAATGAGGTGAACGCAGAACGGATGAAGC TCTTACACCAAGTGTCACGAGTCTGGAGAACAGATGGGTTGAGTAGTTGTTCTTATAAATTAGTATCTGT GGAACACAATCCTTTATATATCAACATCACAGTGGATTTCTGGTTTGGTGCATGA >OriGene 5' read for NM_003778 unedited
TACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGGGACCACCTGGCTTCATGTGTG GATTTCCACGGCTCTTGCCCAGAGGCGGGTACACTGTGTTCCAATGTGCCACGGAACTCA CGCAGTGGCACTTTGTGGCTTCATGAAGGAAGAGGCAGGCCACGCAACACTTCCTCCCCA AGCCAAGGAGAAGTATCACTTTTAGAGGCAGAGGAGCGGAAGGCAGTGGGTGTGACCAAA AGTGCCATTTGTTAAAGACTGTTGGAGCAGAACTACTGAGAAAAACCAGGCATTGTATCT TCAGTTGTCATCAAGTTCGCAATCAGATTGGAAAAGCTCAACTTGAAGCTTTCTTGCCTG CAGTGAAGCAGAGAGATAGATATTATTCACGTAATAAAAAACATGGGCTTCAACCTGACT TTCCACCTTTCCTACAAATTCCGATTACTGTTGCTGTTGACTTTGTGCCTGACAGTGGTT GGGTGGGCCACCAGTAACTACTTCGTGGGTGCCATTCAAGAGATTCCTAAAGCAAAGGAG TTCATGGCTAATTTCCATAAGACCCTCATTTTGGGGAAGGGAAAAACTCTGACTAATGAA GCATCCACGAAGAAGGTAGAACTTGACAACTGTCCTTCTGTGTCTCCTTACCTCAGAGGC CAGAGCAAGCTCATTTTCAAACCAGATCTCACTTTGGAAGAAGGTACAGGCAGAAATCCC ANAGTGTCCAGAGGGCCGGTATCGCCCTCAGGAATGTAAAGCTTACAAGAGGGTCGNCAT TCCTCGGTTCCCCACCGGAACAGAGAGAAACACCTGGATGTACCTGGCTGAACATCTTGC ATCCCCTTCCTGCAGAGGCAGCAGCTGGGATATTGGAATCTACCGTCATCCACCAGCCTG AAGGTNNAAAGGTTTAATCGAGCCCAACTCCTTGAATGGGGGGCTTTCTTAAAAGCCCTC AGGAAGAAAATTGGGACTGGTTTTATTCCCGCAA >OriGene 3' read for NM_003778 unedited
GTATGGCAACTTCCAGGGCCAGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT TTTTTGCTTTTTCACATTTTACTACAGAGGTATTTGATAGACATATTTATTATTAGTTAA TATGTAAACTTGTAAACTGCTAATTCATATCCTACTTGTACAAAATCATTTTACAAAAAC TCTTTAAAAACTAATAGAGAATGTATTCTCTGGTGGGCATCACTGGAAGGCATACCTGGG CAGGTCCTCCCCTGAAGGCTATCAGCACCTGCAACCAGCTCAGCTACTGCTGCCTTTGCA GCCGACACTCCACCCTCCTGCTACCTCTTCATTCCCAAGTTCACTGTGTCCTGATTCGTC GCCTTTTCTCCCCTCTTTTGTGGACGCCTTCTCACCTGACACCACCACTTCACAGATGAT TGTACAGGATAAATGAATAACAGTATTGTATCTTCGTACCTTTCTACCTTGGACGAGAAC AACTCTGGTTCTCTCAGACATTCAGCAGTCTTATTTCCTGTGGCCTTTTATCCCATAATA TATTACTTCAAATTTAAATAAATCTCCTTCAACCAGAGTCCTCAAATAGCGTTCATTNTA TCCTTTGAGTCATCCTTTTATATAAAGTCCTTTCAGTATCCATATTACAAATATTTAACC CTCATGATCANAATGACTAAGAAAGCTGTCTTGNTACAACTGTTTTATACTCTACATCAC CAGGAGCTCTGCNTAGAAATACANAAGGNAAAATTCAGCTCACATGAGCTGTAACAGGNT CTTAATGGGNGCTACTATTTTGAAGCTTTTAGCCAAATTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003778 |
| Insert Size | 2290 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003778.3, NP_003769.1 |
| RefSeq Size | 2283 bp |
| RefSeq ORF | 1035 bp |
| Locus ID | 8702 |
| UniProt ID | O60513 |
| Domains | Galactosyl_T_2 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways |
| Gene Summary | This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an exon in the 5' UTR, as compared to variant 1. Both variants 1 and 2 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209493 | B4GALT4 (Myc-DDK-tagged)-Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 (B4GALT4), transcript variant 2 |
CNY 3,656.00 |
|
| RC209493L3 | Lenti ORF clone of Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 (B4GALT4), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209493L4 | Lenti ORF clone of Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 (B4GALT4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG209493 | B4GALT4 (tGFP-tagged) - Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 (B4GALT4), transcript variant 2 |
CNY 5,256.00 |
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