FPGT (NM_003838) Human Untagged Clone

CAT#: SC117706

FPGT (untagged)-Human fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 1



  "NM_003838" in other vectors (4)

CNY 2,950.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal antibody to FPGT (fucose-1-phosphate guanylyltransferase)
    • 100 ul

CNY 6,281.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GFPP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003838, the custom clone sequence may differ by one or more nucleotides


ATGCGTGCTGTGCGGCGCGGTCTCAGGGAAGGTGGGGCTATGGCAGCTGCTAGGGACCCTCCGGAAGTAT
CGCTGCGAGAAGCCACCCAGCGAAAATTGCGGAGGTTTTCCGAGCTAAGAGGCAAACTTGTAGCACGTGG
AGAATTCTGGGACATAGTTGCAATAACAGCGGCTGATGAAAAACAGGAACTTGCTTACAACCAACAGCTG
TCAGAAAAGCTGAAAAGAAAGGAGTTACCCCTTGGAGTTCAATATCACGTTTTTGTGGATCCTGCTGGAG
CCAAAATTGGAAATGGAGGATCAACACTTTGTGCCCTTCAATGTTTGGAAAAGCTATATGGAGATAAATG
GAATTCTTTTACCATCTTATTAATTCACTCTGGTGGCTACAGTCAACGACTTCCAAATGCAAGTGCTCTG
GGAAAAATTTTCACTGCTTTACCTCTTGGTAACCCCATTTATCAGATGCTAGAATTAAAACTAGCCATGT
ACATTGATTTCCCCTTAAATATGAATCCTGGAATTCTGGTTACCTGTGCAGATGATATTGAACTTTATAG
TATTGGAGAATTTGAGTTTATTAGGTTTGACAAACCTGGCTTTACTGCTTTAGCTCATCCTTCTAGTTTG
ACGATAGGTACCACACATGGAGTATTTGTCTTAGATCCTTTTGATGATTTAAAACATAGAGACCTTGAAT
ACAGGTCTTGCCATCGTTTCCTTCATAAGCCCAGCATAGAAAAGATGTATCAGTTTAATGCTGTGTGTAG
ACCTGGAAATTTTTGTCAACAGGACTTTGCTGGGGGTGACATTGCCGATCTTAAATTAGACTCTGACTAT
GTCTACACAGATAGCCTATTTTATATGGATCATAAATCAGCAAAAATGTTACTTGCTTTTTATGAAAAAA
TAGGCACACTGAGCTGTGAAATAGATGCCTATGGTGACTTTCTGCAGGCTTTGGGACCTGGAGCAACTGT
GGAGTACACCAGAAACACATCAAATGTCATTAAAGAAGAGTCAGAGTTGGTAGAAATGAGGCAGAGAATA
TTTCATCTTCTTAAAGGAACATCACTAAATGTTGTTGTTCTTAATAACTCCAAATTTTATCACATTGGAA
CAACCGAAGAATATTTGTTTTACTTTACCTCAGATAACAGTTTAAAGTCAGAGCTCGGCTTACAGTCCAT
AACTTTTAGTATCTTTCCAGATATACCAGAATGCTCTGGCAAAACATCCTGTATCATTCAAAGCATACTG
GATTCAAGATGTTCTGTGGCACCTGGCTCAGTTGTGGAGTATTCCAGATTGGGGCCTGATGTTTCAGTTG
GGGAAAACTGCATTATTAGTGGTTCTTACATCCTAACAAAAGCTGCCCTCCCCGCACATTCTTTTGTATG
TTCCTTAAGCTTAAAGATGAATAGATGCTTAAAGTATGCAACTATGGCATTTGGAGTGCAAGACAACTTG
AAAAAGAGTGTGAAAACATTGTCAGATATAAAGTTACTTCAATTCTTTGGAGTCTGTTTCCTGTCATGCT
TAGATGTTTGGAATCTTAAAGTTACAGAGGAACTGTTCTCTGGTAACAAGACATGTCTGAGTTTGTGGAC
TGCACGCATTTTCCCAGTTTGTTCTTCTTTGAGTGACTCAGTTATAACATCCCTAAAGATGTTAAATGCT
GTTAAGAACAAGTCAGCATTCAGCCTGAATAGCTATAAGTTGCTGTCCATTGAAGAAATGCTTATCTACA
AAGATGTAGAAGATATGATAACTTACAGGGAACAAATTTTTCTAGAAATCAGTTTAAAAAGCAGTTTGAT
GTAG


>OriGene 5' read for NM_003838 unedited
TTCGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGAAGGT
GGGGCTATGGCAGCTGCTAGGGACCCTCCGGAAGTATCGCTGCGAGAAGCCACCCAGCGA
AAATTGCGGAGGTTTTCCGAGCTAAGAGGCAAACTTGTAGCACGTGGAGAATTCTGGGAC
ATAGTTGCAATAACAGCGGCTGATGAAAAACAGGAACTTGCTTACAACCAACAGCTGTCA
GAAAAGCTGAAAAGAAAGGAGTTACCCCTTGGAGTTCAATATCACGTTTTTGTAGATCCT
GCTGGAGCCAAAATTGGAAATGGAGGATCAACACTTTGTGCCCTTCAATGTTTGGAAAAG
CTATATGGAGATAAATGGAATTCTTTTACCATCTTATTAATTCACTCTGATGAATGGAAG
AAAAAAGTCAGTGAATCATATGTTATCACAATAGAAAGATTAGAAGATGACCTGCAGATC
AAGGAAAAAGAACTGACAGAACTAAGGAATATATTTGGCTCTGATGAAGCCTTCAGTAAA
GTCAATTTAAATTACCGCACTGAAAATGGGCTGTCTCTACTTCATTTATGTTGCATTTGT
GGAGGCAAGAAATCACATATTCGAACTCTTATGTTGAAAGGGCTCCGCCCATCTCGACTG
ACAAGAAATGGATTTACAGCCTTGCATTTAGCAGTTTACAAGGACAAAATATTCCAGTAG
AGTAAGAGGCTTATCAGTTAAATACAGCANAAGTATGTCCTCTCAAGTTTTTCCTTGAGA
CTCGATCAGGANAACCACTATGGNAAGTCAGAAGTGAAGTACAGACGTTGGAGTCCACAG
ACCTGGGTTTGCATCTACCTTCACACTTACAAGATATGCAGAATGATCACCCTCTGCTCA
CAGTGGACTGATTACAGCAGGTGNATACGTGGCCTCT
Restriction Sites NotI-NotI     
ACCN NM_003838
Insert Size 3590 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003838.2, NP_003829.2
RefSeq Size 3426 bp
RefSeq ORF 1785 bp
Locus ID 8790
UniProt ID O14772
Protein Pathways Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
Gene Summary L-fucose is a key sugar in glycoproteins and other complex carbohydrates since it may be involved in many of the functional roles of these macromolecules, such as in cell-cell recognition. The fucosyl donor for these fucosylated oligosaccharides is GDP-beta-L-fucose. There are two alternate pathways for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-D-mannose to GDP-beta-L-fucose. The protein encoded by this gene participates in an alternate pathway that is present in certain mammalian tissues, such as liver and kidney, and appears to function as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. This pathway involves the phosphorylation of L-fucose to form beta-L-fucose-1-phosphate, and then condensation of the beta-L-fucose-1-phosphate with GTP by fucose-1-phosphate guanylyltransferase to form GDP-beta-L-fucose. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream TNNI3 interacting kinase (TNNI3K) gene. [provided by RefSeq, Dec 2010]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...