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USP13 (NM_003940) Human Untagged Clone
CAT＃: SC117705
USP13 (untagged)-Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13)
CNY 6,328.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IsoT-3; ISOT3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117705 sequence for NM_003940 edited (data generated by NextGen Sequencing)
ATGCAGCGCCGGGGCGCCCTGTTCGGCATGCCGGGCGGCAGCGGAGGCAGGAAGATGGCT GCAGGAGACATCGGCGAGCTGCTAGTGCCCCACATGCCCACGATCCGCGTGCCCAGGTCC GGCGACAGGGTCTACAAGAACGAGTGCGCCTTCTCCTACGACTCTCCCAATTCTGAAGGT GGACTCTATGTATGCATGAATACATTTTTGGCCTTTGGAAGGGAACATGTTGAAAGACAT TTTCGAAAAACTGGACAGAGTGTATACATGCACCTGAAAAGACATGTGCGAGAGAAGGTA AGAGGGGCGTCTGGTGGAGCGTTACCAAAAAGGAGGAATTCCAAGATTTTTTTAGATCTA GATACTGATGACGATTTAAATAGCGACGATTATGAATATGAAGATGAAGCCAAACTTGTT ATATTCCCAGATCACTATGAAATAGCACTACCAAATATTGAGGAGTTACCAGCCCTGGTA ACAATTGCTTGTGATGCAGTTCTCAGCTCAAAATCTCCATACAGAAAGCAGGACCCAGAC ACGTGGGAAAATGAATTGCCAGTATCTAAATATGCCAACAACCTCACCCAGCTGGACAAT GGAGTCAGGATTCCTCCAAGTGGTTGGAAGTGTGCCAGATGCGACCTGCGAGAAAACCTC TGGTTGAATCTGACTGACGGCTCTGTCCTGTGTGGAAAGTGGTTCTTTGACAGCTCTGGG GGCAACGGGCATGCGCTGGAGCATTACAGAGACATGGGCTACCCACTAGCCGTGAAACTG GGAACCATCACTCCTGACGGGGCAGATGTTTATTCTTTTCAAGAAGAAGAACCTGTTTTG GATCCTCATTTGGCCAAGCACTTAGCGCATTTTGGAATTGATATGCTTCATATGCATGGG ACAGAGAATGGGCTCCAGGACAATGACATCAAGCTGAGGGTCAGTGAGTGGGAAGTGATC CAGGAGTCGGGCACGAAACTGAAGCCAATGTATGGTCCTGGCTACACGGGTCTGAAGAAC CTGGGCAACAGCTGCTATCTCAGCTCTGTCATGCAGGCCATCTTCAGCATCCCAGAATTC CAGAGAGCGTATGTAGGAAACCTTCCCAGAATATTTGACTACTCGCCTTTAGATCCAACA CAAGATTTCAACACACAGATGACTAAGTTAGGACATGGCCTTCTCTCAGGCCAGTATTCA AAGCCTCCGGTGAAATCTGAACTCATTGAACAGGTGATGAAGGAGGAGCACAAGCCACAG CAGAACGGGATCTCTCCGCGCATGTTTAAGGCCTTTGTAAGCAAGAGCCACCCGGAATTC TCCTCTAACAGGCAGCAAGATGCCCAGGAATTCTTCTTGCACCTGGTGAATCTAGTAGAG AGGAACCGCATCGGCTCAGAAAACCCAAGCGATGTTTTTCGTTTTTTGGTGGAAGAACGC ATTCAGTGCTGTCAGACCCGGAAAGTCCGCTACACGGAGAGGGTGGATTACCTGATGCAG TTACCTGTGGCCATGGAGGCGGCAACCAACAAGGATGAACTGATCGCTTATGAACTAACG AGAAGGGAAGCAGAAGCAAACAGAAGACCCCTTCCTGAGTTGGTACGTGCCAAGATACCA TTTAGTGCCTGCCTTCAGGCCTTCTCTGAACCAGAAAATGTTGATGATTTCTGGAGCAGT GCCCTACAAGCAAAGTCTGCGGGTGTGAAAACATCTCGCTTTGCTTCATTCCCTGAATAC TTGGTAGTGCAGATAAAGAAGTTCACTTTTGGTCTTGACTGGGTTCCCAAAAAATTTGAT GTTTCTATTGATATGCCAGACCTACTTGATATCAACCATCTCCGAGCCAGGGGGTTACAG CCAGGAGAGGAAGAACTTCCAGACATCAGCCCCCCCATAGTCATTCCTGATGACTCAAAA GATCGCCTGATGAACCAATTGATAGACCCATCAGACATCGATGAGTCATCAGTGATGCAG CTGGCCGAGATGGGTTTCCCGCTGGAAGCATGTCGCAAGGCTGTGTACTTCACTGGAAAT ATGGGCGCCGAGGTGGCCTTCAACTGGATCATTGTTCACATGGAAGAGCCAGATTTTGCT GAGCCGCTGACCATGCCTGGTTATGGAGGGGCAGCTTCTGCTGGAGCCTCTGTTTTTGGT GCTTCTGGACTGGATAACCAACCTCCAGAGGAAATCGTAGCTATCATCACCTCCATGGGA TTTCAGCGAAATCAGGCTATTCAGGCACTACGAGCAACGAATAATAACCTGGAAAGAGCA CTGGATTGGATCTTTAGCCACCCTGAGTTTGAAGAAGACAGTGATTTTGTGATTGAGATG GAGAATAATGCCAATGCAAACATTATTTCTGAGGCCAAGCCCGAAGGACCTAGAGTCAAG GATGGATCTGGAACATATGAGCTATTTGCATTCATCAGTCACATGGGAACATCCACAATG AGTGGTCATTACATTTGCCATATCAAAAAGGAAGGAAGATGGGTGATTTACAATGACCAC AAAGTTTGTGCCTCAGAAAGGCCCCCTAAAGACCTGGGCTACATGTACTTTTACCGCAGG ATACCAAGCTAA Clone variation with respect to NM_003940.2 >OriGene 5' read for NM_003940 unedited
TATTATGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGCAGCTGGGCC GGAGACTCGCCATTGGATTAAAAAAGCGTCGCCAGTCCGCCCCAAGCCCGCGGTGCCCGC TCCCGCCCCGCAGCCCGCTCTCCCCGCCCGCCCCGGCTCGGCCGGCTGCCGTTGCCCGCG CAGCCCGCCCGTCAGCCCGCTCGCTGGCGCCGCCGCCGCCGGCAGACCCCGCGCTCCGGC TCCGGCTCGGCTCGCTCGGCTCCGGTGCGCGCCGAGGCCATGCAGCGCCGGGGCGCCCTG TTCGGCATGCCGGGCGGCAGCGGAGGCAGGAAGATGGCTGCAGGAGACATCGGCGAGCTG CTAGTGCCCCACATGCCCACGATCCGCGTGCCCAGGTCCGGCGACAGGGTCTACAAGAAC GAGTGCGCCTTCTCCTACGACTCTCCCAATTCTGAAGGTGGACTCTATGTATGCATGAAT ACATTTTTGGCCTTTGGAAGGGAACATGTTGAAAGACATTTTCGAAAAACTGGACAGAGA CTATACATGCACCTGAAAAGACATGTGCGAGAGAAGGTAAGAGGGGCGTCTGGTGGAGCG TTACCAAAAAGGAGGAATTCCAAGAATTTTTTAGATCTAGATACTGATGACGATTTAAAT AGCGACGATTATGAATATGAAGATGAAGCCAAACTTGTTATATTCCCAGATCACTATGAA ATAGCACTACCAAATATTGAGGAGGTACCAGCCCTGGTAACAATTGCTTGTGATGCAGTT CTCAGCTCAAAATCTCCATACAGAAAGCAGGACCCAGACACGTGGGAAAAATGAATTGCC AGTATCTAAATATGCCAACAACCTCACCCACCTGGACAATGGAGTCAGGATTCTCTCCAG TGGGTTGGAAGTGTGCCACATGCGACCTGCGAGAAAACTTCTGGTGAATCTGACTGAACG CTCTGTNCTGTGTGGAAATTGTTTCTTGACACT >OriGene 3' read for NM_003940 unedited
GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGCAAATTAAAAAGG CGTATGGACTTCTTTTCGCCAATTTTTATATTTGAGGTTTAACTTGGTATACTGCGGTAA AAGTACATGTAGCCCAGGTCTTTAGGGGGCCTTTCTGAGGCACAAACTTTGTGGACATTG AAAATTACCCATCTAACTTACTTTTTGATATGGCAACAGAAATGACCACTCATTGTGGAT GTTTCCATGTGACTGATGAATGCAAATAGCTCATATGCTCCAAATACATACTTGACTCTA GGACCTTCAGTCTTGGACTCACAAATAATGATATGCTCTGCAGTTATTGTGCATCTAAAT GACAAAATCACTGTCTTCTTAAAACTAAGGGATGGCTAAATATTCAACACAAGGCTCACT ATACGCTATTATAACATGCTTTAAATGCTTGCAACACACCGACTACCTTAAAATTCCATG GAGGTGATGATTCCTACAATATATATTGACGGCCTGCCTTATCACCCTCCAACTCCTTAA AACAGACGCTTCCACTTATAACCTGTCTCCTTCATAACCACACCTGGTAACACACTGAAA CACAACACGGTATTTTCCTGTTAACTATGATCCCATTATATTGCTTCTTTCTTATTCATA TTCTCCATCTAAATACCCATCCTTACTTACTGTCCCCTCATGATTAACTTTATTTCCTCC CATCTACTACCGTCAACTTTAAACTATATCACGGTTTTCTCATTCGCTCCTACCGCCACC TTTATACATTACGCAATCACTCTCGCGGATCTTAAATTGCAAAACAAACTCATCCCCCCA CACCACACCCACAAAACGTTCCACCTCACCGCTTCGCCTCCCTACCAACCCAAACATTGT TACCCCACAATCCTCCACCTACTTCCTATCGCTCCCCCCCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003940 |
| Insert Size | 2950 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003940.1, NP_003931.1 |
| RefSeq Size | 2738 bp |
| RefSeq ORF | 2592 bp |
| Locus ID | 8975 |
| UniProt ID | Q92995 |
| Domains | UBA, UCH, zf-UBP |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
USP13 controls the stability of Aurora B impacting progression through the cell cycle
,Esposito, M;Akman, HB;Giron, P;Ceregido, MA;Schepers, R;Ramos Paez, LC;La Monaca, E;De Greve, J;Coux, O;De Trez, C;Lindon, C;Gutierrez, GJ;,
Oncogene
,PubMed ID 32772043
[USP13]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202190 | USP13 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13) |
CNY 6,320.00 |
|
| RC202190L1 | Lenti ORF clone of Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), Myc-DDK-tagged |
CNY 8,720.00 |
|
| RC202190L2 | Lenti ORF clone of Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), mGFP tagged |
CNY 5,890.00 |
|
| RC202190L3 | Lenti ORF clone of Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202190L4 | Lenti ORF clone of Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), mGFP tagged |
CNY 5,890.00 |
|
| RG202190 | USP13 (tGFP-tagged) - Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13) |
CNY 7,920.00 |
