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PAG3 (ASAP2) (NM_003887) Human Untagged Clone
CAT＃: SC117694
ASAP2 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1
CNY 7,016.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_003887 edited
ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCG CCCACGGCCTCCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTGGCGGCCATC GAGGAGGCTTTGGACGTGGACCGGATGGTTCTTTACAAAATGAAGAAATCCGTGAAAGCA ATCAACAGCTCTGGGCTGGCTCACGTGGAAAATGAAGAGCAGTACACCCAGGCTCTGGAG AAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATTTAGGAAGTGCGTTCCTGAAG TTCTCAGTGTTTACAAAGGAGTTGACAGCACTTTTCAAAAACCTGATTCAGAATATGAAC AACATAATCTCCTTCCCTTTGGACAGTTTGCTGAAGGGGGACCTGAAAGGAGTGAAAGGG GATCTGAAAAAGCCTTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAGATA GAAAAGGAGAAAAAGGAACACGCCAAGCTCCATGGGATGATTCGGACTGAAATAAGCGGA GCGGAAATTGCCGAAGAGATGGAAAAGGAGAGGCGCTTCTTCCAGCTACAGATGTGCGAG TATCTGCTGAAGGTCAACGAAATCAAGATTAAAAAGGGAGTAGATTTACTTCAGAATCTG ATCAAATACTTTCATGCCCAATGCAATTTTTTTCAGGATGGACTCAAAGCCGTGGAAAGC CTCAAACCTTCCATTGAAACGCTGTCTACGGATCTTCACACGATCAAACAGGCCCAGGAT GAAGAAAGAAGGCAGTTGATACAGCTTCGAGATATTTTGAAATCCGCATTGCAGGTTGAA CAGAAAGAGTCTAGGAGAGACTCCCAAATTCGTCAGAGCACAGCTTATAGCTTACATCAG CCTCAGGGAAACAAGGAACATGGGACCGAGCGGAACGGCAGCCTCTACAAGAAGAGTGAC GGGATCCGAAAAGTGTGGCAGAAAAGGAAATGTTCAGTTAAAAATGGTTTTCTGACCATA TCCCATGGTACCGCTAACCGGCCTCCTGCAAAGCTCAACCTGCTAACCTGCCAGGTGAAG ACCAACCCTGAGGAGAAGAAGTGCTTTGACCTTATTTCACATGACAGAACTTACCACTTT CAAGCTGAAGATGAACAGGAATGTCAAATATGGATGTCTGTGCTGCAAAATAGCAAAGAA GAAGCTTTAAACAATGCATTTAAGGGGGATGACAATACTGGAGAAAATAACATCGTCCAA GAACTGACAAAGGAGATCATCTCAGAAGTGCAGAGGATGACGGGCAATGACGTCTGCTGT GACTGTGGGGCGCCAGATCCTACATGGCTTTCCACCAACCTGGGCATCCTGACCTGCATC GAGTGTTCCGGAATCCACCGAGAGCTGGGGGTTCATTATTCCAGGATGCAGTCCCTGACC TTAGATGTACTGGGAACATCTGAGCTGCTGCTCGCCAAGAATATTGGGAATGCAGGCTTT AATGAGATCATGGAATGTTGCCTACCAGCTGAGGACTCAGTCAAACCCAACCCAGGCAGC GACATGAATGCAAGAAAGGACTACATCACAGCCAAGTACATCGAGAGGAGATACGCAAGG AAGAAGCACGCGGATAACGCGGCGAAGCTTCACAGTCTTTGCGAGGCCGTCAAAACGAGA GATATTTTTGGATTGCTCCAAGCTTATGCTGATGGTGTGGATCTTACGGAAAAAATCCCA CTGGCCAACGGACATGAGCCGGATGAAACGGCCCTCCACCTTGCAGTCAGATCCGTGGAT CGAACCTCTCTTCACATTGTAGACTTTTTAGTTCAGAACAGTGGGAACCTGGATAAACAG ACAGGGAAAGGCAGCACAGCCCTGCACTACTGCTGCCTGACCGACAATGCCGAGTGCCTC AAGTTGCTCCTGCGGGGGAAGGCCTCCATCGAGATAGCAAACGAGTCAGGAGAGACTCCG CTGGACATTGCCAAGCGCCTCAAGCACGAGCACTGTGAGGAGCTGCTGACCCAAGCCTTA TCTGGAAGATTTAATTCTCACGTTCACGTTGAATATGAATGGCGACTACTCCACGAAGAC CTGGATGAAAGTGATGACGACATGGATGAGAAATTGCAGCCCAGTCCCAACCGGCGGGAA GACCGGCCCATCAGCTTCTACCAGCTGGGCTCCAACCAGCTTCAGTCTAACGCTGTATCT TTGGCCAGAGATGCTGCAAACCTTGCCAAGGAGAAGCAGAGGGCTTTCATGCCCAGCATC TTGCAGAATGAGACTTACGGAGCCCTCCTGAGTGGCAGCCCACCTCCCGCCCAGCCTGCA GCCCCCAGCACCACCAGCGCCCCCCCGCTTCCTCCACGGAATGTTGGCAAAGTTCAGACA GCCTCCTCTGCTAACACCCTGTGGAAGACAAACTCTGTAAGTGTGGACGGTGGAAGCCGG CAGCGATCTTCGTCAGATCCGCCAGCTGTCCATCCACCGCTGCCCCCTCTTCGCGTGACA TCTACCAATCCCCTGACCCCCACGCCGCCCCCACCCGTTGCCAAGACGCCCAGCGTAATG GAAGCCTTGAGCCAGCCGAGCAAGCCTGCCCCGCCTGGGATCTCACAGATCAGGCCCCCA CCTCTGCCCCCACAGCCGCCCAGCCGCCTCCCGCAGAAGAAGCCTGCGCCGGGGGCTGAC AAGTCCACCCCACTGACCAACAAAGGCCAACCGAGAGGACCTGTGGATCTCTCTGCAACG GAAGCTCTGGGTCCTCTGTCCAATGCTATGGTCCTGCAGCCCCCTGCACCCATGCCTAGG AAGTCGCAGGCAACCAAGTTGAAGCCTAAGCGGGTGAAAGCGCTCTATAACTGTGTGGCT GACAACCCCGATGAGCTCACCTTCTCCGAGGGGGATGTGATCATCGTGGACGGGGAGGAG GACCAGGAGTGGTGGATTGGCCACATTGATGGAGATCCTGGTCGCAAAGGCGCATTCCCG GTGTCATTTGTGCACTTTATCGCTGACTGA >OriGene 5' read for NM_003887 unedited
GGGCGGCCGCGTAATTCGCACGAGGGCCCTCGGCGCGCAGGCGGGCGGACCGGCCGAGCT GCGCGGGGCTGCGCGCCGCCCCTGCTCCGCCGCCAGGCCCCGCGCGGCTCCCGCGCCCGG CGCTCCCCTTTGTCCGCGGGCCGGAGCGGCGGCGGCAGCGGCGGTGTCCGAGCGGCGGTC GGAGCCTGCTGCGGCAGTTGAGGCGGCGGCGCCCCTGCGGCTGTGCGCCAGCGCCCTCGC GCCGAGGCGATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAAGAC TACAAGGCGCCCACGGCCTCCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTG GCGGCCATCGAGGAGGCTTTGGACGTGGACCGGATGGTTCTTTACAAAATGAAGAAATCC GTGAAAGCAATCAACAGCTCTGGGCTGGCTCACGTGGAAAATGAAGAGCAGTACACCCAG GCTCTGGAGAAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATTTAGGAAGTGCG TTCCTGAAGTTCTCAGTGTTTACAAAGGGAGTGACAGCACTTTTCACAAACCTGATTCAG AATATGAACAACATAATCTCCTTCCCTTTGGACAGTTTGCTGAATGGGGACCTGAATAGA GTGAAAGGGGATCTGAAAAAGCCTTTTGNATAAGCCTGGGAGGACTATGAAACANAAATT ACCCAGATCAGAAACGGAGAAAAGGGACACGCCAAGCTCCATGGGATGATTCGGACTGAA ATAAGCCGAGCGGAAATTGCCGATAGATGGAAAAGGAGAGCGCTTTCTTCAGCTACAGAT GTGCGAGTATCTGCTGAAGGTCACGAAATCCAGATTAAGAAGGAAGTAGATTTACTTCAA AATCCGATCAAATACTTTCATGCCCATGCAATTTTTT >OriGene 3' read for NM_003887 unedited
CTTTGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTCCCATTAAAAAGAATTTTATTTTTTTCCAGTCAAATGACTAGTTAACAAAAAAA GTAACCTTATTAAACATGCTCTAATTATAAATCACTGCTTTAAGGACAATGAAAATAATC AATTTCGGTTATACAATATATACAGTTGGGCTGCACCCAAAGTAATCAGGGGAATGAACT GAATATCATACATCTAAAAATAGCATCCTAAGCTGCATATTATGTTATCCACCCCTTAAC AAATCACACAGTTACTTTAAGTCTGGGACCATGTTCTGACCCATCATCCCAAATCTGATG GAAAATGGCATGCAAAATGCCAAAATCCTGCAGCTGCAGTTCATGAAACATAAACTTTAA ATATAAATAAATATCTACAAGGTTTTTCTTTCTCTTAGTTGCTTTTTTAATTGGCAGGGA GCAAATACCTAAAAAGGGATTTTACCAGGGCCGTTAATATAATTTCTCCTCTGGAAAAAG TACTATTAGTTACTGCACAATAGCACCAAATTGTAAACTGGAAAAATATTTCCTAGGTAT TTATGTCCCAGGGACCCTGACAAATTATTTGGGGACTGGAGTTCTAATGTTGATATGAAT CAACTTTATTCCTTATATAGCTGGCACCAGGTGGTCAAAAGGCTGCCTATAAAAACAGAT GCAGGAGGATGGTTAAAATAGACATAAGGGCCCTGTGCAAAACACATCTGGTTACTTAAA TCTATGAAAAAATAATGGGAGGGTTCAAACGAAATAACATTAGTGAAACCAGATGTGGTG CTTAAAAAAAACAAAACCAAACCTCCGATTTACCTTTTTCCCGGACCTTTGAGGTAAAAG CGATGCTTACACATATAAAATTTCCGTTTGTTTGTTTCCTTTACAAAAAAAAGACAGCCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003887 |
| Insert Size | 5270 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003887.1, NP_003878.1 |
| RefSeq Size | 5711 bp |
| RefSeq ORF | 3021 bp |
| Locus ID | 8853 |
| UniProt ID | O43150 |
| Domains | ArfGap, SH3, PH, ANK |
| Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
| Gene Summary | This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221783 | ASAP2 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1 |
CNY 7,008.00 |
|
| RC221783L3 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1, Myc-DDK-tagged |
CNY 11,970.00 |
|
| RC221783L4 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1, mGFP tagged |
CNY 11,970.00 |
|
| RG221783 | ASAP2 (tGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1 |
CNY 8,608.00 |
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