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PLK3 (NM_004073) Human Untagged Clone
CAT＃: SC117577
PLK3 (untagged)-Human polo-like kinase 3 (PLK3)
CNY 7,224.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CNK; FNK; PLK-3; PRK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_004073 edited
ATGGAGCCTGCCGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCGTGCGGCCGCCGCGCCC GCTCCCCCGGCCGGGCCCGGGCCGCCTCCGAGTGCCTTGCGCGGACCTGAGCTGGAGATG CTGGCCGGGCTACCGACGTCAGACCCCGGGCGCCTCATCACGGACCCGCGCAGCGGCCGC ACCTACCTCAAAGGCCGCTTGTTGGGCAAGGGGGGCTTCGCCCGCTGCTACGAGGCCACT GACACAGAGACTGGCAGCGCCTACGCTGTCAAAGTCATCCCGCAGAGCCGCGTCGCCAAG CCGCATCAGCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCGAGACCTGCAGCACCGC CACATCGTGCGTTTTTCGCACCACTTTGAGGACGCTGACAACATCTACATTTTCTTGGAG CTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGGCACACCCTGTTGGAGCCA GAAGTGCGCTACTACCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGCGCGGC ATCTTGCACCGGGACCTCAAGTTGGGAAATTTTTTCATCACTGAGAACATGGAACTGAAG GTGGGGGATTTTGGGCTGGCAGCCCGGTTGGAGCCTCCGGAGCAGAGGAAGAAGACCATC TGTGGCACCCCCAACTATGTGGCTCCAGAAGTGCTGCTGAGACAGGGCCACGGCCCTGAG GCGGATGTATGGTCACTGGGCTGTGTCATGTACACGCTGCTCTGCGGGAGCCCTCCCTTT GAGACGGCTGACCTGAAGGAGACGTACCGCTGCATCAAGCAGGTTCACTACACGCTGCCT GCCAGCCTCTCACTGCCTGCCCGGCAGCTCCTGGCCGCCATCCTTCGGGCCTCACCCCGA GACCGCCCCTCTATTGACCAGATCCTGCGCCATGACTTCTTTACCAAGGGCTACACCCCC GATCGACTCCCTATCAGCAGCTGCGTGACAGTCCCAGACCTGACACCCCCCAACCCAGCT AGGAGTCTGTTTGCCAAAGTTACCAAGAGCCTCTTTGGCAGAAAGAAGAAGAGTAAGAAT CATGCCCAGGAGAGGGATGAGGTCTCCGGTTTGGTGAGCGGCCTCATGCGCACATCCGTT GGCCATCAGGATGCCAGGCCAGAGGCTCCAGCAGCTTCTGGCCCAGCCCCTGTCAGCCTG GTAGAGACAGCACCTGAAGACAGCTCACCCCGTGGGACACTGGCAAGCAGTGGAGATGGA TTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGGAGTCAGCCCTTTGTGCTCTGAGAAAT TGTATAGCCTTCATGCCCCCAGCGGAACAGAACCCGGCCCCCCTGGCCCAGCCAGAGCCT CTGGTGTGGGTCAGCAAGTGGGTTGACTACTCCAATAAGTTCGGCTTTGGGTATCAACTG TCCAGCCGCCGTGTGGCTGTGCTCTTCAACGATGGCACACATATGGCCCTGTCGGCCAAC AGAAAGACTGTGCACTACAATCCCACCAGCACAAAGCACTTCTCCTTCTCCGTGGGTGCT GTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCCTGCGGTACTTCGCCTCCTACATGGAG CAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGTGGAAGAGGTAGAGGTACCTGCTCCG CCCTTGCTGCTGCAGTGGGTCAAGACGGATCAGGCTCTCCTCATGCTGTTTAGTGATGGC ACTGTCCAGGTGAACTTCTACGGGGACCACACCAAGCTGATTCTCAGTGGCTGGGAGCCC CTCCTTGTGACTTTTGTGGCCCGAAATCGTAGTGCTTGTACTTACCTCGCTTCCCACCTT CGGCAGCTGGGCTGCTCTCCAGACCTGCGGCAGCGACTCCGCTATGCTCTGCGCCTGCTC CGGGACCGCAGCCCAGCCTAG >OriGene 5' read for NM_004073 unedited
GCAAGAATTTGTAATACGACNACTATAGGGCGGCCGCGAATTCGCACCAGGCAAATCCAG GCAGCGCCACGCGCGGCCGGGGCCGGGCGGAACCGATAAGCCGGGACCGCGCTGCGACGC GCCGGCCGCATGGAGCCTGCCGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCGTGCGGAC GCCGCGCCCGCTCCCCCGGCCGGGCCCGGGCCGCCTCCGAGTGCCTTGCGCGGACCTGAG CTGGAGATGCTGGCCGGGCTACCGACGTCAGACCCCGGGCGCCTCATCACGGACCCGCGC AGCGGCCGCACCTACCTCAAAGGCCGCTTGTTGGGCAAGGGGGGCTTCGCCCGCTGCTAC GAGGCCACTGACACAGATACTGGCATCGCCTACGCTGTCAAAGTCATCCCGCATAGCCGC GTCGCCAAGCCGCATCATCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCGAGACCTG CAGCACCGCCACATCGTGCGTTTTTCGCACCACTTTGAGGACGCTGACAACATCTACATT TTCTTGGAGCTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAATGCCCGGCACACCCTG TTGGAGCCAGAAGTGCGCTACTACCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCAC CAGCGCGGCATCTTGCACCGGGACCTCAAGTTGTGAAATTCTTTCATCACTGAGAACATG GAACTGAAGGTGGGGGATTNTGAGCTGGCATCCCAGTTGGATCCTTCGGAGCAAAGGATG AGACCATCTGTGGCACCCACAACTATGTGGCTNCATAAGTGCTGCTGATACATGACCACN GNCCTAGAGCGGATGTATGGTCACTGGGCTGTGTCATGTACACACTGCTCTAGCGGAGCC CCTCCCTTGAGACNGCTGACCTGNAGGAGACCTACAGCTGCATCAAGCAGGTTCACTACA CGCTGNCTGGCANNCTNTACTGNCTGGCAGGNAGNTCTGGNCGCTT >OriGene 3' read for NM_004073 unedited
TTTCCGCCAATAGCTTGNACCGCGCCGCTTTCTANNGATCGATTTTTTTTTTTTTTTTTT TTTTTTCCAATTCTGCAAAATTGTTTATTATCCTAGGAGGAGGCCCCCCTGGGGCTCACA TCCCAATAAATAAGTGTCTGACAATAAATAAAAGTGGTCCATAAATAACGCCCCCTAGCT GGGAGCTAAGGCTCAGGCTTATCTTGGAGATGGGGTAGGAGCCAGCGAAGACAAGCCGCC CCCAACTCCAGTAAAGCTGGTCCCTGATTCCCTGGGGGATTCGGCCCAAAGCCCCCAGTG AGGCACCAAAGGAAGGGGGAAGGCCACAAAGGCAAGGGCCAGAGCCTGACAGGCACAGGC CTCAGGCCTCAGGGCTTGGGTCCTAGGCTGGGCTGCGGTCCCGGAGCAGGCGCAGAATCA TAGCGGAGTCGCTGCCGCAGGTCTGGAGAGCAGCCCAGCTGCCGAAGGTGGGAAGCGAGG TAAGTACAAACACTACGATTTCGGGCCACAAAAGTCACAAGGAGGGGCTCCCAGCCACTG AGAATCAGCTTGGTGTGGTCCCCGTAGAAGTTCACCTGGACAGTGCCATCACTAAACAGC ATGAGGAGAGCCTGATCCGTCTTGACCCACTGCAGCAGCAAGGGCGGAGCAGGTACCTCT ACCTCTTCCACACTGGGCAGATCTCCACCCTTCATGAGGTGCTGCTCCATGTAGGAGGCG AAGTACCGCAGGATACCCAGCTGAGGCTGCAGGGCCCGGGGCACAGCACCCACGGAGAAG GAGAAGTGCTTTGTGCTGGTGGGATTGTAGTGCACAGTCTTTCTGTTGGCCGACAGGGCC ATATGTGTGCCATCGTTGAAGACACAGCCACACGGCGCTGGACAGTTGATCCCAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004073 |
| Insert Size | 2200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004073.2, NP_004064.2 |
| RefSeq Size | 2369 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 1263 |
| UniProt ID | Q9H4B4 |
| Domains | pkinase, POLO_box, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
In-cell NMR Spectroscopy in Mammalian Cells
,Bekei, B;,
Thesis
[PLK3]
|
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Resources
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