TRIP12 (NM_004238) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MRD49; TRIP-12; TRIPC; ULF
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_004238, the custom clone sequence may differ by one or more nucleotides ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC AACCACAAGACGACTCAATAGGAGGAAGAAGCTGCAGTTCATCATCTGCTGTGATAGTTCCACAACCAGA GGATCCAGACAGAGCCAATACTTCAGAAAGACAAAAAACGGGGCAGGTGCCTAAGAAAGACAATTCTCGA GGAGTGAAGCGCAGTGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCAAAAAAACCAAAAG CACTTCAGCATACTGAATCTCCCTCAGAAACAAATAAGCCACATAGTAAGTCAAAGAAGAGACATTTAGA CCAGGAGCAACAACTGAAATCTGCACAATCACCATCAACAAGCAAGGCTCATACCAGGAAGAGTGGGGCC ACTGGCGGTTCACGGAGTCAGAAAAGAAAAAGGACAGAGAGTTCTTGTGTAAAGAGTGGCTCCGGGTCTG AATCAACTGGTGCAGAAGAGAGATCTGCGAAACCTACCAAGCTGGCTTCAAAATCAGCCACCTCAGCCAA AGCTGGGTGTAGCACCATCACTGATTCTTCTTCTGCTGCCTCTACTTCCTCCTCGTCTTCTGCTGTAGCC TCGGCCTCCTCCACTGTACCACCAGGTGCCAGAGTGAAACAAGGAAAAGATCAGAACAAGGCCAGGCGTT CCCGTTCAGCGTCCAGTCCCAGCCCCAGAAGAAGTAGCAGGGAAAAGGAACAGAGTAAAACTGGTGGCTC TTCAAAATTTGATTGGGCTGCTCGTTTCAGCCCTAAAGTTAGCCTTCCTAAAACAAAACTGTCTCTTCCA GGGTCTTCTAAGTCAGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTAGCAAGTTTAAGAA AATCTACGAAGAAACGCAGTGAGTCTCCACCTGCTGAGCTCCCCAGTTTGAGGCGGAGCACACGCCAAAA GACCACGGGCTCCTGTGCTAGTACCAGTCGGCGAGGCTCTGGCCTGGGCAAAAGAGGAGCAGCTGAAGCT CGTCGACAGGAGAAAATGGCAGACCCTGAAAGCAACCAGGAGGCAGTAAATTCTTCAGCTGCTCGGACAG ATGAAGCTCCCCAAGGAGCTGCAGGGGCTGTTGGCATGACCACCTCTGGGGAGAGTGAATCAGATGATTC CGAGATGGGACGTTTGCAAGCTTTGTTAGAGGCAAGGGGTCTTCCCCCTCACCTATTTGGTCCTCTTGGT CCTCGGATGTCACAGCTTTTCCATAGAACAATTGGAAGTGGAGCTAGTTCTAAGGCCCAGCAGCTACTAC AAGGATTGCAAGCCAGTGATGAAAGTCAACAGCTTCAGGCAGTTATTGAGATGTGTCAGTTACTGGTCAT GGGAAATGAGGAGACACTGGGAGGGTTTCCTGTCAAGAGTGTTGTTCCAGCTTTGATTACGTTACTTCAG ATGGAGCACAATTTTGATATTATGAACCATGCTTGTCGAGCCTTAACATACATGATGGAAGCACTTCCTC GATCTTCTGCTGTTGTAGTAGATGCTATTCCTGTCTTTTTAGAAAAGCTGCAAGTTATTCAGTGTATTGA TGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGAGACATAGTAAAGCCATTCTACAGGCG GGTGGTTTGGCAGACTGCTTGCTGTACCTAGAATTCTTCAGCATAAATGCCCAAAGAAATGCATTAGCAA TTGCAGCTAATTGCTGCCAGAGTATCACGCCAGATGAATTTCATTTTGTGGCAGATTCACTCCCATTGCT AACCCAAAGGCTAACACATCAGGATAAAAAGTCAGTAGAAAGCACTTGCCTTTGTTTTGCACGCCTAGTG GACAACTTCCAGCATGAGGAGAATTTACTCCAGCAGGTTGCTTCCAAAGATCTGCTTACAAATGTTCAAC AGCTGTTGGTAGTGACTCCACCCATTTTAAGTTCTGGGATGTTTATAATGGTGGTTCGCATGTTTTCTCT GATGTGTTCCAACTGTCCAACTTTAGCTGTTCAACTTATGAAACAAAACATTGCAGAAACGCTTCACTTT CTCCTGTGTGGTGCCTCCAATGGAAGTTGTCAGGAACAGATTGATCTTGTTCCACGAAGCCCTCAAGAGT TGTATGAACTGACATCTCTGATTTGTGAACTTATGCCATGTTTACCAAAAGAAGGCATTTTTGCAGTTGA TACCATGTTGAAGAAGGGAAATGCACAGAACACAGATGGTGCGATATGGCAGTGGCGTGATGATCGGGGC CTCTGGCATCCATATAACAGGATTGACAGCCGGATCATTGAGCAAATCAATGAGGACACGGGAACAGCAC GTGCCATTCAGAGAAAACCTAACCCGTTAGCCAATAGTAACACTAGTGGATATTCAGAGTCAAAGAAGGA TGATGCTCGAGCACAGCTTATGAAAGAGGATCCGGAACTGGCTAAGTCTTTTATTAAGACATTATTTGGT GTTCTTTATGAAGTGTATAGTTCCTCAGCAGGACCTGCGGTCAGACATAAGTGCCTTAGAGCAATTCTTA GGATAATTTATTTTGCGGATGCTGAACTTCTGAAGGATGTTCTGAAAAATCATGCTGTTTCAAGTCACAT TGCTTCCATGCTGTCAAGCCAAGACCTGAAGATAGTAGTGGGAGCACTTCAGATGGCAGAAATTTTAATG CAGAAGTTACCTGATATTTTTAGTGTTTACTTCAGAAGAGAAGGTGTAATGCATCAAGTAAAACACTTAG CAGAATCAGAGTCTTTGTTGACAAGTCCACCAAAGGCATGTACGAATGGATCGGGATCCATGGGATCCAC AACTTCAGTCAGCAGTGGGACAGCCACAGCTGCCACTCATGCTGCAGCTGACTTGGGATCACCCAGCTTG CAGCACAGCAGGGATGATTCTTTAGATCTCAGCCCTCAAGGTCGATTAAGTGATGTTCTAAAGAGAAAAC GACTGCCAAAACGAGGGCCAAGAAGGCCAAAGTACTCACCTCCAAGAGATGATGACAAAGTAGACAATCA AGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCTTTCCTGGCAAGCTTGAATCCAAAAACATGG GGAAGGTTAAGTACACAGTCCAACAGCAACAACATTGAGCCAGCACGGACTGCGGGAGGTAGTGGCCTTG CCAGGGCTGCCTCAAAGGATACCATCTCCAATAATAGAGAAAAAATTAAAGGTTGGATTAAGGAGCAGGC ACATAAATTTGTAGAACGTTATTTCAGTTCTGAGAATATGGATGGAAGCAACCCTGCATTGAATGTCCTT CAGAGACTTTGTGCTGCAACCGAACAACTCAACCTCCAGGTGGATGGTGGAGCTGAGTGCCTTGTAGAAA TCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGAAATCCAACATAGTGGATTTGTGAAGCAGCT GTTGCTTTATTTGACATCTAAAAGTGAAAAGGATGCTGTGAGCAGAGAGATCAGATTAAAGCGATTTCTT CATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCCATTGGAAGAGTGGAACCAGTGGGTAATGCAC CTTTGTTGGCATTAGTTCACAAGATGAACAACTGCCTCAGCCAGATGGAACAATTTCCAGTCAAAGTACA TGATTTCCCTAGTGGAAATGGGACAGGAGGCAGCTTTTCTCTCAACAGAGGATCACAGGCTTTAAAATTT TTCAACACACATCAATTAAAATGCCAGTTACAAAGGCATCCAGACTGTGCAAATGTGAAGCAGTGGAAGG GTGGACCTGTCAAGATTGACCCTCTGGCTTTGGTACAAGCCATCGAGAGATACCTTGTAGTTAGAGGGTA TGGAAGAGTAAGAGAAGATGATGAAGACAGCGATGACGATGGATCAGATGAGGAAATAGATGAGTCTCTG GCTGCTCAGTTCCTAAATTCAGGAAATGTAAGACACAGGCTGCAGTTTTATATTGGAGAACATTTGCTGC CGTATAACATGACTGTGTATCAGGCAGTACGGCAGTTTAGTATACAGGCTGAAGATGAAAGAGAATCCAC AGATGATGAGAGCAATCCTCTAGGCAGAGCTGGTATTTGGACAAAGACTCATACAATATGGTATAAACCT GTGAGAGAGGATGAAGAAAGTAATAAAGATTGTGTTGGTGGTAAAAGAGGAAGAGCCCAAACAGCTCCAA CGAAAACTTCCCCTAGAAATGCAAAAAAGCATGATGAGTTATGGCACGATGGAGTGTGCCCATCAGTATC AAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAAATATAACATTTGAAGACCCGTCATTAGAT GTGATCCTTCTTTTAAGAGTTTTACATGCTATCAGTCGATACTGGTATTACTTGTATGATAATGCAATGT GCAAGGAAATTATTCCAACTAGTGAATTTATTAACAGTAAGTTAACAGCAAAAGCAAATAGGCAACTTCA AGATCCTTTAGTAATCATGACAGGAAACATCCCAACATGGCTTACTGAGCTAGGAAAAACCTGCCCATTT TTCTTTCCTTTTGATACCCGGCAAATGCTTTTTTATGTAACTGCATTTGATCGGGACCGAGCAATGCAAA GATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCTCAAGATAGCAGAGTTGCACCTAGATTGGA TAGAAAAAAACGTACTGTGAACCGAGAGGAGCTGCTGAAACAGGCGGAGTCTGTGATGCAGGACCTCGGC AGCTCACGGGCCATGTTAGAAATCCAGTATGAAAATGAGGTTGGTACAGGTCTTGGGCCTACACTGGAGT TTTATGCGCTTGTATCTCAGGAACTACAGAGAGCTGACTTGGGTCTTTGGAGAGGTGAAGAAGTAACTCT TAGCAATCCAAAAGGGAGCCAAGAAGGGACCAAGTATATTCAAAACCTCCAGGGCCTGTTTGCGCTTCCC TTTGGTAGGACAGCAAAGCCAGCTCATATCGCAAAGGTTAAGATGAAGTTTCGCTTCTTAGGAAAATTAA TGGCCAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCCCTTGGCTTACCCTTTTATAAATGGATGCT ACGGCAAGAAACTTCACTGACATCACACGATTTGTTTGACATCGACCCAGTTGTAGCCAGATCAGTTTAT CACCTAGAAGACATTGTCAGACAGAAGAAAAGACTTGAACAAGATAAATCCCAGACCAAAGAGAGTCTAC AGTATGCATTAGAAACCTTGACTATGAATGGCTGCTCAGTTGAAGATCTAGGACTGGATTTCACTCTGCC AGGGTTTCCCAATATCGAACTGAAGAAAGGAGGGAAGGATATACCAGTCACTATCCACAATTTAGAGGAG TATCTAAGACTGGTTATATTCTGGGCACTAAATGAAGGCGTTTCTAGGCAATTTGATTCGTTCAGAGATG GATTTGAATCAGTCTTCCCACTCAGTCATCTTCAGTACTTCTACCCGGAGGAACTGGATCAGCTCCTTTG TGGCAGTAAAGCAGACACTTGGGATGCAAAGACACTGATGGAATGCTGTAGGCCTGATCATGGTTATACT CATGACAGTCGGGCTGTGAAGTTTTTGTTTGAGATTCTCAGTAGTTTTGATAATGAGCAGCAGAGGTTAT TTCTCCAGTTTGTGACTGGTAGCCCAAGATTGCCTGTTGGAGGATTCCGGAGTTTGAATCCACCTTTGAC AATTGTCCGAAAGACGTTTGAATCAACAGAAAACCCAGATGACTTCTTGCCCTCTGTAATGACTTGTGTG AACTATCTTAAGTTGCCGGACTATTCAAGCATTGAGATAATGCGTGAAAAACTGTTGATAGCAGCAAGAG AAGGGCAGCAGTCGTTCCATCTTTCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_004238 unedited AGTATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGGAGCTAGT GGAAGTTACTGCCGCGCCACCGAGTCCGGACCGGAGACTTTGGGGCCTAACTAGAGTCTC ACTCTGTCACCCAGGCTGGAGTGCAGTGGCGTGATCTCAGCTCAGTGCAACCTCTGCCTC CCAGGTTCAGGTGATTCTCCTGCCTCAGCCTCCTCAGTAGCTGGGACCACAGAGACGGGG TTTCACCATGGTCTTGATCTCCTGATCTTGTGATCCGCCTGCCTCGGCCTCCCAAAGTGC TGGGATTACAAGTGTGAGCCACCGCGCCCGGCCTAAAATCTCTTAAACTGAATTGTGAGA CGTTACGCGGATGCTTCATAAGATGACCAACTGTTTGGAATAAAGAATATGTGAATGGTA GTGTCTAGAAAGGGTATGTCCCTTCAAGAGAGAGGTGCCAATGTCCAACCGGCCTAATAA CAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCCAACCACAAGA CGACTCAATAGGAGGAAGGTCACATTTAGGGCAGGCAAAACATAAGGGATATAGCCCTCC TGAGAGTAGAAAATCTAATTCTAAGGCACCCAAAGTGCAGTCTAATACTACTTCTGAACT GTCAAGGGGACACCTTTCTAAAAGAAGCTGCAGTTCATCATCTGCTGTGATAGTTCCACA ACCAGAGGATCCAGACAGAGCCAATACTTCAGAAAGACANAAAACGGNGCAGGTGCCTAA GAAAGACCATTCTCGAGGAGTGAAGCGCAGTGCTAGTCCAGACTACAACAGGACCAATTC TCCTAGCTCTGCAAAAAAACCAAAAGCACTTCAGCATACTGAATCTNCCTCAGNNAACAA TAGCCACATTAGTAGTCAAAGAAGAGACATTTAGACAGGNAGCACACTGGAACTGCACAT CACCTCAACAGC
Restriction Sites	NotI-NotI
ACCN	NM_004238
Insert Size	4910 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_004238.1, NP_004229.1
RefSeq Size	6428 bp
RefSeq ORF	5979 bp
Locus ID	9320
UniProt ID	Q14669
Domains	HECT
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (3) encodes isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.