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RUNX1T1 (NM_004349) Human Untagged Clone
CAT＃: SC117449
RUNX1T1 (untagged)-Human runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 1
CNY 6,456.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004349, the custom clone sequence may differ by one or more nucleotides
ATGCCTGATCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAGACGCAATCTAGGC TGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAGAACCAGTTCATTTACACCGAC AACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGCCCCCTCACCACCCAATGGCTTC AGCAATGGGCCTTCCTCTTCTTCCTCCTCCTCTCTGGCTAATCAACAGCTGCCCCCAGCCTGTGGTGCCA GGCAACTCAGCAAGCTGAAAAGGTTCCTTACTACCCTGCAGCAGTTTGGCAATGACATTTCACCCGAGAT AGGAGAAAGAGTTCGCACCCTCGTTCTGGGACTAGTGAACTCCACTTTGACAATTGAAGAATTTCATTCC AAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCATTTTTGAAGGCCAACTTGCCCC TGCTGCAGCGTGAGCTCCTCCACTGCGCAAGACTGGCCAAACAGAACCCTGCCCAGTACCTCGCCCAGCA TGAACAGCTGCTTCTGGATGCCAGCACCACCTCACCTGTTGACTCCTCAGAGCTGCTTCTCGATGTGAAC GAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTTGACAGAGAGCCTTTGCACTCAG AACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTACAGTCCAAATAACGGCTTATCCTA CCAGCCCAATGGCCTGCCTCACCCTACCCCACCTCCACCTCAGCATTACCGTTTGGATGATATGGCCATT GCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGACCTCAGGGACAGAAACAGACCTATGG GGTTGCATGGCACACGTCAAGAAGAAATGATTGATCACAGACTAACAGACAGAGAATGGGCAGAAGAGTG GAAACATCTTGACCATCTGTTAAACTGCATAATGGACATGGTAGAAAAAACAAGGCGATCTCTCACCGTA CTAAGGCGGTGTCAAGAAGCAGACCGGGAAGAATTGAATTACTGGATCCGGCGGTACAGTGACGCCGAGG ACTTAAAAAAAGGTGGCGGCAGTAGCAGCAGCCACTCTAGGCAGCAGAGTCCCGTCAACCCAGACCCAGT TGCACTAGACGCGCATCGGGAATTCCTTCACAGGCCTGCGTCTGGATACGTGCCAGAGGAGATCTGGAAG AAAGCTGAGGAGGCCGTCAATGAGGTGAAGCGCCAGGCGATGACGGAGCTGCAGAAGGCCGTGTCTGAGG CGGAGCGGAAAGCCCACGACATGATCACAACAGAGAGGGCCAAGATGGAGCGCACGGTCGCCGAGGCCAA ACGGCAGGCGGCGGAGGACGCACTGGCAGTTATCAATCAGCAGGAGGATTCAAGCGAGAGTTGCTGGAAT TGTGGCCGTAAAGCGAGTGAAACCTGCAGTGGCTGTAACACAGCCCGATACTGTGGCTCATTTTGCCAGC ACAAAGACTGGGAGAAGCACCATCACATCTGTGGACAGACCCTGCAGGCCCAGCAGCAGGGAGACACACC TGCAGTCAGCTCCTCTGTCACGCCCAACAGCGGGGCTGGGAGCCCGATGGACACACCACCAGCAGCCACT CCGAGGTCAACCACCCCGGGAACCCCTTCCACCATAGAGACAACCCCTCGCTAG >OriGene 5' read for NM_004349 unedited
CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGACACAATG TGCCATCCTGACAAGGCTTTTACTTCTGATAAGCTCCAATGTGTGTTTAATGAATACAAA GCCGCGGTCTGGGTGCCGCCTCGGCCGCGGCCGCTCTCCCGCGCTCCTTTGCCAGAAGCT GGCGGAGAGAATTTACATTTAAAGATTAGCAGAGTGAGAAAGAGAAATCTGCCTTTTGTT GTGTGGGGTGAGGAGGAGGCATCTACCCCTGGCCTTGACGCTATCTCCCATCACCTCTGC TATCCAGACAGGACTCACCGAGAAAAGATTGATCTCTGGGCTGGTGAACATAATCTCTGT CCCAGTCAGAAAAGGAGAGAGGAAATTAGCAGAGCGATTGGTGGAGAATGATATCTGTCA AAAGAAACACTTGGAGAGCACTGAGTTTAGTAATAGGTGACTGCCGGAAAAAAGGGAACT TTGAATATTGTCAAGGCGCACGCGCCCAGCACCCTGCCTCCGGCGATGCCGCCCCAGCCC CTCTGATGGCCCTCCTCTCTGCTGCCACTCATTCCAGAACAGGAGGCATGAGCCCGGAAC GCGCTTGCTTTTAGGAGACAGCCACTTTCTGTGTGGTACGCTGGATTCAAGGATGCCTGA TCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAGACGCAATCTAG GCTGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAGAACCAGTTC ATTTACACCCGACACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGC CCCCTCACCACCCATGGCTTCAGCAATGGGCCCTTNCTCTTCTTCTCCTNCTCTCTGGCT AATCACAGCTGCCCCCAGCCTGTGGTGCCCAGCACT >OriGene 3' read for NM_004349 unedited
NNNNNNNNNNNNNTTTTTGACTTTGGACCGCGGNCCGCATNCTAGGATCGAGTTTTTTTT TTTTTTTTTTCCTTATTGTATATAAAGAACTTGTGACTTTTAATATTAGCATTTTTGCTT TCAACAGCAATTAGCAATCTCTTGGTTTGCTGTTTGGTAAAGCATCGGTTAATGAGGTCA TCTAGTTTAAAATCCCAGCTACTTGAAAATAACAGGGAGGAGGTCAAATCTATCATTTCA TGGTATATTCTCTGCTCTCTTTTCAGTTCTCTAAAGAAAAGATATCTTTGTTATCCCACA TAAGTCATTACGACCCCGTACTGGCCCTTTGGTGTTTACTACCACCTCAAGATCACGTAG GTCCCTTTTTGCTTGAGTCCTGGAATTGGTAATTCTTCTTTTTACCACCACCCCAAAAAC AACTTTAAACATCTAAGGATGAGGAATGGTTTCCCGTGGTGGGGTTGGCCTTCCTCCACA GTTCTAGATTCACGTTATCCAGGGGTTGTCTTTTGTGGGAAAGGGGTCCCCGGGGGGGGT ACCCTCCGAGGGGTTCTGGGGGGGGGCCCCCCGCCCCCCACCCCCTTTTTTGCCGTACAA AAAACCCCCCTCGGGGTTTTCTCCTTTTTTGTGCCCCCCGAGGTCTTCCCCAAATAGAAG GGCCTCTTCACAACTTTGCCTGGCAAATTGACCCAAAATTTGGCTTGTTTCCACCCCCTG AGGTTTACTTCTTTTTAGGCCATTTCCACACAACCCCCTTGAACCCCCCGGTAAGGAAAA ATGGAAGGGCCCTCGCCCCTTGCCCTTGCCCTGGACCCGCGCCCCATTTGGCCCTTTTTT GGAAAATGGGGGGGTTTTCCTCCCCTAAAACGCCTTTGCACTCCTTTTCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004349 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004349.2, NP_004340.1 |
| RefSeq Size | 7420 bp |
| RefSeq ORF | 1734 bp |
| Locus ID | 862 |
| UniProt ID | Q06455 |
| Domains | zf-MYND, TAFH |
| Protein Families | Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Pathways in cancer |
| Gene Summary | This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (1) encodes isoform A. Variants 1, 5 and 6 all encode isoform A, also known as MTG8a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218564 | RUNX1T1 (Myc-DDK-tagged)-Human runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 1 |
CNY 5,800.00 |
|
| RC218564L3 | Lenti-ORF clone of RUNX1T1 (Myc-DDK-tagged)-Human runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 1 |
CNY 7,700.00 |
|
| RC218564L4 | Lenti-ORF clone of RUNX1T1 (mGFP-tagged)-Human runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 1 |
CNY 7,700.00 |
|
| RG218564 | RUNX1T1 (tGFP-tagged) - Human runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 1 |
CNY 6,370.00 |
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