AGL (NM_000028) Human Untagged Clone

CAT#: SC117442

AGL (untagged)-Human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4



  "NM_000028" in other vectors (6)

CNY 10,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Monoclonal antibody against AGL
    • 100 ul

CNY 5,236.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GDE
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_000028, the custom clone sequence may differ by one or more nucleotides


ATGGGACACAGTAAACAGATTCGAATTTTACTTCTGAACGAAATGGAGAAACTGGAAAAGACCCTCTTCA
GACTTGAACAAGGGTATGAGCTACAGTTCCGATTAGGCCCAACTTTACAGGGAAAAGCAGTTACCGTGTA
TACAAATTACCCATTTCCTGGAGAAACATTTAATAGAGAAAAATTCCGTTCTCTGGATTGGGAAAATCCA
ACAGAAAGAGAAGATGATTCTGATAAATACTGTAAACTTAATCTGCAACAATCTGGTTCATTTCAGTATT
ATTTCCTTCAAGGAAATGAGAAAAGTGGTGGAGGTTACATAGTTGTGGACCCCATTTTACGTGTTGGTGC
TGATAATCATGTGCTACCCTTGGACTGTGTTACTCTTCAGACATTTTTAGCTAAGTGTTTGGGACCTTTT
GATGAATGGGAAAGCAGACTTAGGGTTGCAAAAGAATCAGGCTACAACATGATTCATTTTACCCCATTGC
AGACTCTTGGACTATCTAGGTCATGCTACTCCCTTGCCAATCAGTTAGAATTAAATCCTGACTTTTCAAG
ACCTAATAGAAAGTATACCTGGAATGATGTTGGACAGCTAGTGGAAAAATTAAAAAAGGAATGGAATGTT
ATTTGTATTACTGATGTTGTCTACAATCATACTGCTGCTAATAGTAAATGGATCCAGGAACATCCAGAAT
GTGCCTATAATCTTGTGAATTCTCCACACTTAAAACCTGCCTGGGTCTTAGACAGAGCACTTTGGCGTTT
CTCCTGTGATGTTGCAGAAGGGAAATACAAAGAAAAGGGAATACCTGCTTTGATTGAAAATGATCACCAT
ATGAATTCCATCCGAAAAATAATTTGGGAGGATATTTTTCCAAAGCTTAAACTCTGGGAATTTTTCCAAG
TAGATGTCAACAAAGCGGTTGAGCAATTTAGAAGACTTCTTACACAAGAAAATAGGCGAGTAACCAAGTC
TGATCCAAACCAACACCTTACGATTATTCAAGATCCTGAATACAGACGGTTTGGCTGTACTGTAGATATG
AACATTGCACTAACGACTTTCATACCACATGACAAGGGGCCAGCAGCAATTGAAGAATGCTGTAATTGGT
TTCATAAAAGAATGGAGGAATTAAATTCAGAGAAGCATCGACTCATTAACTATCATCAGGAACAGGCAGT
TAATTGCCTTTTGGGAAATGTGTTTTATGAACGACTGGCTGGCCATGGTCCAAAACTAGGACCTGTCACT
AGAAAGCATCCTTTAGTTACCAGGTATTTTACTTTCCCATTTGAAGAGATAGACTTCTCCATGGAAGAAT
CTATGATTCATCTGCCAAATAAAGCTTGTTTTCTGATGGCACACAATGGATGGGTAATGGGAGATGATCC
TCTTCGAAACTTTGCTGAACCGGGTTCAGAAGTTTACCTAAGGAGAGAACTTATTTGCTGGGGAGACAGT
GTTAAATTACGCTATGGGAATAAACCAGAGGACTGTCCTTATCTCTGGGCACACATGAAAAAATACACTG
AAATAACTGCAACTTATTTCCAGGGAGTACGTCTTGATAACTGCCACTCAACACCTCTTCACGTAGCTGA
GTACATGTTGGATGCTGCTAGGAATTTGCAACCCAATTTATATGTAGTAGCTGAACTGTTCACAGGAAGT
GAAGATCTGGACAATGTCTTTGTTACTAGACTGGGCATTAGTTCCTTAATAAGAGAGGCAATGAGTGCAT
ATAATAGTCATGAAGAGGGCAGATTAGTTTACCGATATGGAGGAGAACCTGTTGGATCCTTTGTTCAGCC
CTGTTTGAGGCCTTTAATGCCAGCTATTGCACATGCCCTGTTTATGGATATTACGCATGATAATGAGTGT
CCTATTGTGCATAGATCAGCGTATGATGCTCTTCCAAGTACTACAATTGTTTCTATGGCATGTTGTGCTA
GTGGAAGTACAAGAGGCTATGATGAATTAGTGCCTCATCAGATTTCAGTGGTTTCTGAAGAACGGTTTTA
CACTAAGTGGAATCCTGAAGCATTGCCTTCAAACACAGGTGAAGTTAATTTCCAAAGCGGCATTATTGCA
GCCAGGTGTGCTATCAGTAAACTTCATCAGGAGCTTGGAGCCAAGGGTTTTATTCAGGTGTATGTGGATC
AAGTTGATGAAGACATAGTGGCAGTAACAAGACACTCACCTAGCATCCATCAGTCTGTTGTGGCTGTATC
TAGAACTGCTTTCAGGAATCCCAAGACTTCATTTTACAGCAAGGAAGTGCCTCAAATGTGCATCCCTGGC
AAAATTGAAGAAGTAGTTCTTGAAGCTAGAACTATTGAGAGAAACACGAAACCTTATAGGAAGGATGAGA
ATTCAATCAATGGAACACCAGATATCACAGTAGAAATTAGAGAACATATTCAGCTTAATGAAAGTAAAAT
TGTTAAACAAGCTGGAGTTGCCACAAAAGGGCCCAATGAATATATTCAAGAAATAGAATTTGAAAACTTG
TCTCCAGGAAGTGTTATTATATTCAGAGTTAGTCTTGATCCACATGCACAAGTCGCTGTTGGAATTCTTC
GAAATCATCTGACACAATTCAGTCCTCACTTTAAATCTGGCAGCCTAGCTGTTGACAATGCAGATCCTAT
ATTAAAAATTCCTTTTGCTTCTCTTGCCTCCAGATTAACTTTGGCTGAGCTAAATCAGATCCTTTACCGA
TGTGAATCAGAAGAAAAGGAAGATGGTGGAGGGTGCTATGACATACCAAACTGGTCAGCCCTTAAATATG
CAGGTCTTCAAGGTTTAATGTCTGTATTGGCAGAAATAAGACCAAAGAATGACTTGGGGCATCCTTTTTG
TAATAATTTGAGATCTGGAGATTGGATGATTGACTATGTCAGTAACCGGCTTATTTCACGATCAGGAACT
ATTGCTGAAGTTGGTAAATGGTTGCAGGCTATGTTCTTCTACCTGAAGCAGATCCCACGTTACCTTATCC
CATGTTACTTTGATGCTATATTAATTGGTGCATATACCACTCTTCTGGATACAGCATGGAAGCAGATGTC
AAGCTTTGTTCAGAATGGTTCAACCTTTGTGAAACACCTTTCATTGGGTTCAGTTCAACTGTGTGGAGTA
GGAAAATTCCCTTCCCTGCCAATTCTTTCACCTGCCCTAATGGATGTACCTTATAGGTTAAATGAGATCA
CAAAAGAAAAGGAGCAATGTTGTGTTTCTCTAGCTGCAGGCTTACCTCATTTTTCTTCTGGTATTTTCCG
CTGCTGGGGAAGGGATACTTTTATTGCACTTAGAGGTATACTGCTGATTACTGGACGCTATGTAGAAGCC
AGGAATATTATTTTAGCATTTGCGGGTACCCTGAGGCATGGTCTCATTCCTAATCTACTGGGTGAAGGAA
TTTATGCCAGATACAATTGTCGGGATGCTGTGTGGTGGTGGCTGCAGTGTATCCAGGATTACTGTAAAAT
GGTTCCAAATGGTCTAGACATTCTCAAGTGCCCAGTTTCCAGAATGTATCCTACAGATGATTCTGCTCCT
TTGCCTGCTGGCACACTGGATCAGCCATTGTTTGAAGTCATACAGGAAGCAATGCAAAAACACATGCAGG
GCATACAGTTCCGAGAAAGGAATGCTGGTCCCCAGATAGATCGAAACATGAAGGACGAAGGTTTTAATAT
AACTGCAGGAGTTGATGAAGAAACAGGATTTGTTTATGGAGGAAATCGTTTCAATTGTGGCACATGGATG
GATAAAATGGGAGAAAGTGACAGAGCTAGAAACAGAGGAATCCCAGCCACACCAAGAGATGGGTCTGCTG
TGGAAATTGTGGGCCTGAGTAAATCTGCTGTTCGCTGGTTGCTGGAATTATCCAAAAAAAATATTTTCCC
TTATCATGAAGTCACAGTAAAAAGACATGGAAAGGCTATAAAGGTCTCATATGATGAGTGGAACAGAAAA
ATACAAGACAACTTTGAAAAGCTATTTCATGTTTCCGAAGACCCTTCAGATTTAAATGAAAAGCATCCAA
ATCTGGTTCACAAACGTGGCATATACAAAGATAGTTATGGAGCTTCAAGTCCTTGGTGTGACTATCAGCT
CAGGCCTAATTTTACCATAGCAATGGTTGTGGCCCCTGAGCTCTTTACTACAGAAAAAGCATGGAAAGCT
TTGGAGATTGCAGAAAAAAAATTGCTTGGTCCCCTTGGCATGAAAACTTTAGATCCAGATGATATGGTTT
ACTGTGGAATTTATGACAATGCATTAGACAATGACAACTACAATCTTGCTAAAGGTTTCAATTATCACCA
AGGACCTGAGTGGCTGTGGCCTATTGGGTATTTTCTTCGTGCAAAATTATATTTTTCCAGATTGATGGGC
CCGGAGACTACTGCAAAGACTATAGTTTTGGTTAAAAATGTTCTTTCCCGACATTATGTTCATCTTGAGA
GATCCCCTTGGAAAGGACTTCCAGAACTGACCAATGAGAATGCCCAGTACTGTCCTTTCAGCTGTGAAAC
ACAAGCCTGGTCAATTGCTACTATTCTTGAGACACTTTATGATTTATAG


>OriGene 5' read for NM_000028 unedited
GCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGAGGCCGCGCCTTGG
CCGGTGCACCTTCCCCAGGCAAGGAGAAAGCGCCGCTCCCGGCCTCAGCCGCAGCAGGCT
CCAGGTCCCCCGGCCCGGAGCCGACTGAGACGGTGCGGTGCCCACGCTCTCGCGAGACTA
GCGGTCGGGGCGGGCGGGTCGAGCCTCCCGGAAGTGGGCCAGAGGGGTAACTCATTCGAC
TGTGGAGTTCTTTTAATTCTTATGAAAGATTTCAAATCCTCTAGAAGCCAAAATGGGACA
CAGTAAACAGATTCGAATTTTACTTCTGAACGAAATGGAGAAACTGGAAAAGACCCTCTT
CAGACTTGAACAAGGGTATGAGCTACAGTTCCGATTAGGCCCAACTTTACAGGGAAAAGC
AGTTACCGTGTATACAAATTACCCATTTCCTGGAGAAACATTTAATAGAGAAAAATTCCG
TTCTCTGGATTGGGAAAATCCAACAGAAAGAGAAGATGATTCTGATAAATACTGTAAACT
TAATCTGCAACAATCTGGTTCATTTCAGTATTATTTCCTTCAAGGAAATGAGAAAAGTGG
TGGAGGTTACATAGTTGTGGACCCCNATTTTACGTGTGGTGCTGATAATCATGTGCTACC
CTTGGACTGTGTTACTCTTCAGACTTNNTAGCCTAGTGTTTGGGACCTTTTGATGAATGG
GAAAGCAGACTTAAGGTTGCAAAGAATCAGGCTACACATGATTCATTNTACCCCATTGCA
GACTCTTGGACTATCTAGGTCATGCTACTCCCTTGNCNATCAGTTAGAATTAATTCTGAC
TTTTTCAGACCTATANAAAGTTACCTGGAATGGATGTTTGAACAGCTAGTGGAAG
>OriGene 3' read for NM_000028 unedited
TAGCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGACCTTT
AGAAAATATTTTTATTTTCTTAACACACAGAGGTAACAATGACATTTGCCCACTAGACAC
AGTAGTTCAAGACATATCAGAAATGGATCACAATTCACATGTTACAATATTCATCAATAT
GACCAGTCTCATGTTTTCCCAATTATCTTTGAAAACTTATTAAAATACCACAGCATACTA
AACTGGTAAATTCATTTATCATGTTAAAGAGTAATTTTGATTTTGATTAGTGAAAAACCA
AGGCATAGTCCATTGTTGAAATACAATACCTTATCCAGCATCACTCAAGTATGAAGCATT
CTACCGAAACGTTTAGTCAAATGACACCTGTTAAAAATCTAATTCTGTATTTTAACTACC
ATAGAGGAAATAATGTTTAACATGCCTTCTTAAACTTTAAAATATACCACGCAGAATATA
ACAATGTTCTTTCATTAATCTTCCTCATTAGAAATATGAACCACTTAGTTTTCTAGTGCA
ACACAATATCCTAAAAATTTACCAACGTGGGTATCTGCCTTACTAGAGATAACCCCAAAT
GGCTACATTAAAAAAATTGTGAACTTTTAATTTTTTTGGTCACATTTAACTGTCAGCGAT
TACTTCTTGCTTTGATTAAATATGCTGGGTTTCTGTTACCTTTCCTACCTCCCCTGATAT
CATAGTTAGGGTACAGCACCAAAACTACAAAACCTTTGATAAACCAATGGTTCTTTTAAA
GGCAGTCATTTCTTTTACCCGCCAGTTTAAAGAAGGGATTTTTTGACCAGGAAGGAACCT
GAGAACAAAGAAGATTTGTAAACCATGGCCATGTTTTATAGATGTGGTCAAATAACGGCC
AACTTGCCTTAATTGGGAGAATTAACCATTTTTATTTTGAAACACAAACTATCTTGTTAA
N
Restriction Sites NotI-NotI     
ACCN NM_000028
Insert Size 7070 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000028.1, NP_000019.1
RefSeq Size 7445 bp
RefSeq ORF 4599 bp
Locus ID 178
UniProt ID P35573
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Starch and sucrose metabolism
Gene Summary This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (1). Variants 1, 2, 3 and 4 encode the same isoform (isoform 1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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