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CDC25B (NM_004358) Human Untagged Clone
CAT＃: SC117412
CDC25B (untagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 2
CNY 9,120.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004358, the custom clone sequence may differ by one or more nucleotides
ATGGAGGTGCCCCAGCCGGAGCCCGCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTGGCGCCC AGCGTCCGGGCCACCTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGTCCCCGGTGCGGGCGGC CGCTTCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACCTCGCCGGGCTCGGCAGCCGCAGCCGC CTGACGCACCTATCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTGAATCCTCCGAATCTTCTG ATGCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCCACATGGCGGAGCAGACGTTTGAACAGGC CATCCAGGCAGCCAGCCGGATCATTCGAAACGAGCAGTTTGCCATCAGACGCTTCCAGTCTATGCCGGTG AGGCTGCTGGGCCACAGCCCCGTGCTTCGGAACATCACCAACTCCCAGGCGCCCGACGGCCGGAGGAAGA GCGAGGCGGGCAGTGGAGCTGCCAGCAGCTCTGGGGAAGACAAGGAGAATGATGGATTTGTCTTCAAGAT GCCATGGAAGCCCACACATCCCAGCTCCACCCATGCTCTGGCAGAGTGGGCCAGCCGCAGGGAAGCCTTT GCCCAGAGACCCAGCTCGGCCCCCGACCTGATGTGTCTCAGTCCTGACCGGAAGATGGAAGTGGAGGAGC TCAGCCCCCTGGCCCTAGGTCGCTTCTCTCTGACCCCTGCAGAGGGGGATACTGAGGAAGATGATGGATT TGTGGACATCCTAGAGAGTGACTTAAAGGATGATGATGCAGTTCCCCCAGGCATGGAGAGTCTCATTAGT GCCCCACTGGTCAAGACCTTGGAAAAGGAAGAGGAAAAGGACCTCGTCATGTACAGCAAGTGCCAGCGGC TCTTCCGCTCTCCGTCCATGCCCTGCAGCGTGATCCGGCCCATCCTCAAGAGGCTGGAGCGGCCCCAGGA CAGGGACACGCCCGTGCAGAATAAGCGGAGGCGGAGCGTGACCCCTCCTGAGGAGCAGCAGGAGGCTGAG GAACCTAAAGCCCGCGTCCTCCGCTCAAAATCACTGTGTCACGATGAGATCGAGAACCTCCTGGACAGTG ACCACCGAGAGCTGATTGGAGATTACTCTAAGGCCTTCCTCCTACAGACAGTAGACGGAAAGCACCAAGA CCTCAAGTACATCTCACCAGAAACGATGGTGGCCCTATTGACGGGCAAGTTCAGCAACATCGTGGATAAG TTTGTGATTGTAGACTGCAGATACCCCTATGAATATGAAGGCGGGCACATCAAGACTGCGGTGAACTTGC CCCTGGAACGCGACGCCGAGAGCTTCCTACTGAAGAGCCCCATCGCGCCCTGTAGCCTGGACAAGAGAGT CATCCTCATTTTCCACTGTGAATTCTCATCTGAGCGTGGGCCCCGCATGTGCCGTTTCATCAGGGAACGA GACCGTGCTGTCAACGACTACCCCAGCCTCTACTACCCTGAGATGTATATCCTGAAAGGCGGCTACAAGG AGTTCTTCCCTCAGCACCCGAACTTCTGTGAACCCCAGGACTACCGGCCCATGAACCACGAGGCCTTCAA GGATGAGCTAAAGACCTTCCGCCTCAAGACTCGCAGCTGGGCTGGGGAGCGGAGCCGGCGGGAGCTCTGT AGCCGGCTGCAGGACCAGTGA >OriGene 5' read for NM_004358 unedited
TGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGGTGCCCCAGCCGGAGC CCGCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTGGCGCCCAGCGTCCGGGCC ACCTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGTCCCCGGTGCGGGCGGCCG CTTCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACCTCGCCGGGCTCGGCAGCG AAACCCCAAAGAGTCAGGTAGGGACCCTGCTCTTCCGCAGCCGCAGCCGCCTGACGCACC TATCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTGAATCCTCCGAATCTTCTG ATGCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCCACATGGCGGAGCACACGT TTGAACAGGCCATCCAGGCAGCCAGCCGGATCATTCGAAACGAGCAGTTTGCCATCAGAC GCTTCCAGTCTATGCCGGTGAGGCTGCTGGGCCACAGCCCCGTGCTTCGGAACATCACCA ACTCCCAGGCGCCCGACGGCCGGAGGAAGAGCGAGGCGGGCAGTGGAGCTGCCAGCAGCT CTGGGGAAGACAAGGAGAATGATGGATTTGTCTTCAAGATGCCCTGGAAAGCCCACACAT CCCAGCTCCCACCCATGCTCTGGCAGAGTGGGCCAGCCGCAGGGAAGCCTTTGCCCAGAG ACCCAGCTCGGCCCCCGACCTGATGTGTCTCAGTCCTGACCGGAAGATGGAAATGGAGGA GCTCACCCCCCTGGCCCCTAGTCGCTTCTCTCTGACCCTGCCGNNAGGGATACCTGAGAA AGAGATGGCATTTGTGACCTCCCACAGAGGGACTTAAAGGATGATGATGCCACTTCCCCC AGCCATGGAAAGTCTTATTAGTGCCCCCACTGGCAG >OriGene 3' read for NM_004358 unedited
TGTACCGCGGCCCGCTTTTCTATATCGAGTTTTTTTTTTTTTTTTTTCTCAACTTGCTCA TTGCTTTATTGAACCAAGGCCCTAACAGATGACTCAGCAGGGCCTTCAAGCACAGCCCTG CCCCCGTCTTGAGATTCAGAATCCAGAGGGTGCTCAGTCCTTGGTTTATCTGCTTCTGTG ACATTTCCTCTTGAATAGCTACAAACCCTAAGTGTAAATATTTTTGTCCACACAAATAGG CACACATATATTCCTGCTGCAGGCTGTCATGCTCAACAAAACGCTCCCACCTGGTTTGGG TATGCAAGGCACTGCGCATCCACGGCCATCCACGGCCATCCACCCATCCATCCAACCTTC CATCCATCCGCAACAAGACAGCAGCAAGTTCTGAGCACTCAGGCCCCAGGGTTCACAGCA GTAAGGGCTGGGCTTCCATCAGGCTCATGATTCAGGCCTTAACCCTTGCCCTGGGAGCCC CCAATGATAATTAACATCCCTTTCTGGGGCAACAAAACTGGGACCGATTGGGTAACCTGT ATTTGACAGGGGAACCTAAACAGTCTACGGAAGTTGCCTGAGATGGCCGTAAAGCCAAAC GGACCATAGCCACAGGTCACACTGAAATAGGAAAGTAGTAAAGCCTCTGTTCAAGCTGAA GGTGCCTCGTATGGTGGCACCCGACAAGGGGAAAACCAAAGGGGGCCCTCTGGCAATCTT CCCTCGTTTCTCACATAAATAGGTGCCCCCCTGGCCCGAGTTTTCCACAAGCATTAAATC TGCTCTTCAGGCCACGAAGCATCTACCCTCGACCATTCCTTAGTAACACGGTTCTCCCCC ACTCTCCTCGTCCCCCACCTTGGATCCCCTCCGACCCCGCTAAAGAAGGTTCTCCTTACA GATACAAAAACCCTCGGGATCAATATTGTCTTTTCTGTGTTACCACTAATCACTTACATA CGGTCTTCAGGGGGGGGCTCCATATCCCACTCCTATTAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004358 |
| Insert Size | 2930 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004358.3, NP_004349.1 |
| RefSeq Size | 3659 bp |
| RefSeq ORF | 1701 bp |
| Locus ID | 994 |
| UniProt ID | P30305 |
| Domains | RHOD |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation |
| Gene Summary | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2), also known as CDC25B1, uses an alternate splice site in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 2) compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216838 | CDC25B (Myc-DDK-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 2 |
CNY 5,040.00 |
|
| RC216838L3 | Lenti-ORF clone of CDC25B (Myc-DDK-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 2 |
CNY 6,940.00 |
|
| RC216838L4 | Lenti-ORF clone of CDC25B (mGFP-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 2 |
CNY 6,940.00 |
|
| RG216838 | CDC25B (tGFP-tagged) - Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 2 |
CNY 5,510.00 |
