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Glypican 5 (GPC5) (NM_004466) Human Untagged Clone
CAT＃: SC117378
GPC5 (untagged)-Human glypican 5 (GPC5)
CNY 4,264.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117378 sequence for NM_004466 edited (data generated by NextGen Sequencing)
ATGGACGCACAGACCTGGCCCGTGGGCTTTCGCTGCCTCCTCCTTCTGGCCCTGGTTGGG TCCGCCCGCAGCGAGGGCGTGCAGACCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGCGG CTGCTGGGAGCTGTCAGGGGGCTGCCGGATTCGCCGCGGGCAGGACCTGATCTTCAGGTT TGCATATCCAAAAAGCCTACATGTTGCACCAGGAAGATGGAGGAGAGATATCAGATTGCG GCTCGCCAGGATATGCAGCAGTTTCTTCAAACGTCCAGCTCTACATTAAAGTTTCTAATA TCTCGAAATGCGGCTGCTTTTCAAGAAACCCTTGAAACTCTCATCAAACAAGCAGAAAAT TACACCAGTATACTTTTTTGCAGTACCTACAGGAACATGGCCTTGGAGGCTGCTGCTTCG GTTCAGGAGTTCTTCACTGATGTGGGGCTGTATTTATTTGGTGCGGATGTTAATCCTGAA GAATTTGTAAACAGATTTTTTGACAGTCTTTTTCCTCTGGTCTACAACCACCTCATTAAC CCTGGTGTGACTGACAGTTCCCTGGAATACTCAGAATGCATCCGGATGGCTCGCCGGGAT GTGAGTCCATTTGGTAATATTCCCCAAAGAGTAATGGGACAGATGGGGAGGTCCCTGCTG CCCAGCCGCACTTTTCTGCAGGCACTCAATCTGGGCATTGAAGTCATCAACACCACAGAC TATCTGCACTTCTCCAAAGAGTGCAGCAGAGCCCTCCTGAAGATGCAATACTGCCCGCAC TGCCAAGGCCTGGCGCTCACTAAGCCTTGTATGGGATACTGCCTCAATGTCATGCGAGGC TGCCTGGCGCACATGGCGGAGCTTAATCCACACTGGCATGCATATATCCGGTCGTTGGAA GAACTCTCGGATGCAATGCATGGAACATACGACATTGGACACGTGCTGCTGAACTTTCAC TTGCTTGTTAATGATGCTGTGTTACAGGCTCACCTCAATGGACAAAAATTATTGGAACAG GTAAATAGGATTTGTGGCCGCCCTGTAAGAACACCCACACAAAGCCCCCGTTGTTCTTTT GATCAGAGCAAAGAGAAGCATGGAATGAAGACCACCACAAGGAACAGTGAAGAGACGCTT GCCAACAGAAGAAAAGAATTTATCAACAGCCTTCGACTGTACAGGTCATTCTATGGAGGT CTAGCTGATCAGCTTTGTGCTAATGAATTAGCTGCTGCAGATGGACTTCCCTGCTGGAAT GGAGAAGATATAGTAAAAAGTTATACTCAGCGTGTGGTTGGAAATGGAATCAAAGCCCAG TCTGGAAATCCTGAAGTCAAAGTCAAAGGAATTGATCCTGTGATAAATCAGATTATTGAT AAACTGAAGCATGTTGTTCAGTTGTTACAGGGTAGATCACCCAAACCTGACAAGTGGGAA CTTCTTCAGCTGGGCAGTGGTGGAGGCATGGTTGAACAAGTCAGTGGGGACTGTGATGAT GAAGATGGTTGCGGGGGATCAGGAAGTGGAGAAGTCAAGAGGACACTGAAGATCACAGAC TGGATGCCAGATGATATGAACTTCAGTGATGTAAAGCAAATCCATCAAACAGACACTGGC AGTACTTTAGACACAACAGGAGCAGGATGTGCAGTGGCGACTGAATCTATGACATTCACT CTGATAAGTGTGGTGATGTTACTTCCCGGGATTTGGTAA Clone variation with respect to NM_004466.4 >OriGene 5' read for NM_004466 unedited
GTCCCCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGTACACCCC GCAGCCGGCTCGCACCGCTCGAGAGCCTCGGCCGCTGTGTCTTCCACGTCTGCAGCTCAG CCAGGGCGCGCAGGGCGAGTGGGGTCCACTGGCGGGTAAAGGGGACCAGGACGGCGAGGA TGGACGCACAGACCTGGCCCGTGGGCTTTCGCTGCCTCCTCCTTCTGGCCCTGGTTGGGT CCGCCCGCAGCGAGGGCGTGCAGACCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGCGGC TGCTGGGAGCTGTCAGGGGGCTGCCGGATTCGCCGCGGGCAGGACCTGATCTTCAGGTTT GCATATCCAAAAAGCCTACATGTTGCACCAGGAAGATGGAGGAGAGATATCAGATTGCGG CTCGCCAGGATATGCAGCAGTTTCTTCAAACGTCCAGCTCTACATTAAAGTTTCTAATAT CTCGAAATGCGGCTGCTTTTCAAGAAACCCTTGAAACTCTCATCAAACAAGCCGAAAATT ACACCAGTATACTTTTTTGCAGTACCTACAGGAACATGGCCTTGGAGGCTGCTGCTTCGG TTCAGGAGTTCTTCACTGATGTGGGGCTGTATTTATTTGGTGCGGATGTTAATCCTGAAG ATTTGNTAACAGATTTTTTGACAGTCTTTTTCCTCTGGTCTACAACCACCTCATTAACCC TGGTGTGACTGACAGTTCCCTGGAATACTCANAATGCATNCGGATGGCTCGCCGGGATGT GAGTCCATTTTGTAATATTCCCCAAAGAGTATGGGACAGAGGGGNAGTCCCTGCTGCCCA GCCCACTTTTCTGCAGGACTCAATCTGGGCATTGAATCATCACACCACAGACTTCTGCAC TCTTCAAGATGCAGCAGAGCCCTCTGAGATGCATACATGCG >OriGene 3' read for NM_004466 unedited
TTCTTGGATATATCTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTCCACCAAATAGTTAACAGCTTTATTGGAAATAT TCCTAGAGCAAGCATGTGCTTCCCAACATAGCTAAATCTATGAGGCAAAAAGAGAGATGG GAGCAAAGGCCCAATACCAAAGATGCATTTTGAATATAAGGGAATACAAATCAGGGGTTA ATAAAATACTCAGCTTTAAGGTTCATCAGGGGCTAAGTTACAGGGGGTCAAAATTATCAA GGTTCTTCAGGGAAATTATATGAAATGCTTCTCAAAAATCTACCCCATAACTTATAGACA TTCAAACATTTCCTTCTACAGCTTGCAAAAATGGAAAAGTCTCTTGAATATGCATGCATA CTACTTGCCATTGGATGTTTTGATTTATTTCATGATATTCTAATTTCAATACTTTAAAAA ATACTGCATACTGTTTTCATTGGCTGGCAAATACACTTTCAAATGAAAACATTTTTAATT TAAAAAGGAAGGGGCTTGGAATTGATAATAAAATTACAGATCCCCTAAATTCTTCTTATT CAAAGTCACTTCTGTGCCCTTCACATCTTCAGATTTAATATATTAAAAGTTAAGAAGGTT TTCAGTTAGATTGAAAAAAGACATATTTTTAAAGTGTGTCATTTAAACGTTGAGTTTTAG GGACTTTATGAATATTTCAGCTTGGCTTCTGAAAAGTTAGTGTAACATATCTTTTCATAA AATAATTGTTACATTGAAAAAGATCTCTTTATCCAGGCCTATGCAAAAAGGGAAGAAATC GAGACTTTGTTAGGTATTTTCAGGACAGAAAAATTCATA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_004466 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004466.3, NP_004457.1 |
| RefSeq Size | 2934 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 2262 |
| UniProt ID | P78333 |
| Domains | Glypican |
| Gene Summary | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Glypican-5 is a tumor suppressor in non-small cell lung cancer cells
,Guo, L;Wang, J;Zhang, T;Yang, Y;,
Biochemistry and Biophysics Reports
,PubMed ID 27092337
[GPC5]
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|
Glypican-5 stimulates rhabdomyosarcoma cell proliferation by activating Hedgehog signaling
,Fuchuan Li, Wen Shi, Mariana Capurro, and Jorge Filmus,
J. Cell Biol., Feb 2011; 192: 691 - 704
[GPC5]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207909 | GPC5 (Myc-DDK-tagged)-Human glypican 5 (GPC5) |
CNY 4,256.00 |
|
| RC207909L1 | Lenti ORF clone of Human glypican 5 (GPC5), Myc-DDK-tagged |
CNY 6,656.00 |
|
| RC207909L2 | Lenti ORF clone of Human glypican 5 (GPC5), mGFP tagged |
CNY 6,460.00 |
|
| RC207909L3 | Lenti ORF clone of Human glypican 5 (GPC5), Myc-DDK-tagged |
CNY 6,656.00 |
|
| RC207909L4 | Lenti ORF clone of Human glypican 5 (GPC5), mGFP tagged |
CNY 6,460.00 |
|
| RG207909 | GPC5 (tGFP-tagged) - Human glypican 5 (GPC5) |
CNY 5,856.00 |
|
| SC324198 | GPC5 (untagged)-Human glypican 5 (GPC5) |
CNY 4,264.00 |
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