NDUFB2 (NM_004546) Human Untagged Clone

CAT#: SC117303

NDUFB2 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa (NDUFB2), nuclear gene encoding mitochondrial protein



  "NM_004546" in other vectors (4)

CNY 1,200.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


NDUFB2 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AGGG; CI-AGGG
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117303 sequence for NM_004546 edited (data generated by NextGen Sequencing)
ATGTCCGCTCTGACTCGGCTGGCGTCTTTCGCTCGCGTTGGAGGCCGCCTTTTCAGAAGC
GGCTGCGCACGGACTGCTGGAGATGGTGGAGTCCGTCATGCCGGTGGTGGTGTGCACATT
GAGCCCCGGTATAGACAGTTCCCCCAGCTGACCAGATCCCAGGTGTTCCAGAGCGAGTTC
TTCAGCGGACTCATGTGGTTCTGGATTCTCTGGCGCTTTTGGCATGACTCAGAAGAGGTG
CTGGGTCACTTTCCGTATCCTGATCCTTCCCAGTGGACAGATGAAGAATTAGGTATCCCT
CCTGATGATGAAGACTGA

Clone variation with respect to NM_004546.2
>OriGene 5' read for NM_004546 unedited
TACATTTGTATACGACTACTATAGGCGGCCGCGAAATTCGCACGAGGGGACCGCGGGGCG
GACGGGAGCGAGTATGTCCGCTCTGACTCGGCTGGCGTCTTTCGCTCGCGTTGGAGGCCG
CCTTTTCAGAAGCGGCTGCGCACGGACTGCTGGAGATGGTGGAGTCCGTCATGCCGGTGG
TGGTGTGCACATTGAGCCCCGGTATAGACAGTTCCCCCAGCTGACCAGATCCCAGGTGTT
CCAGAGCGAGTTCTTCAGCGGACTCATGTGGTTCTGGATTCTCTGGCGCTTTTGGCATGA
CTCAGAAGAGGTGCTGGGTCACTTTCCGTATCCTGATCCTTCCCAGTGGACAGATGAAGA
ATTAGGTATCCCTCCTGATGATGAAGACTGAAGGTGTAGACTCAGCCTCACTCTGTACAA
GAGCCAGAGCTAGCAACATGGACCACAGATATACTGGTGACTCCGCTCTCCTCTGGCCAA
GCTACAAATCATCACCTGAGGCAGAGATGCTACTACCCTCTTCTGGNGCTCAACTACCGA
TGGGCATCAGTAATCCATCCCAGTATAACATTTCAAGGATGTGCTGGAGACAATACATGG
ACTTTCTCTTTCAATGGCGAGGAATGAAACANGATACAAGAACCACAGCATGGAATATGT
TTTCTTTTACATCCAATGGCCCGGTCCATAAAGGATGCCTTGAGACCTCCACCCTGTCCC
CCACAGCGCTTTGGGACAGGATGACTAACGCAGTCCAACTTATGTGCAATCAGTTCCCGG
GAAAAATTTACTTTTAAAACTTGAAAATCATTGTCTAGTCGGCACAATACTCTGGAACTG
ATAAAACCAATTCGGCCTATATTCAATTCCCCCTCTAATAAAACATGATGCTGCCCACAT
CAGTACTTTACCCTAACTCCCCAATTT
Restriction Sites NotI-NotI     
ACCN NM_004546
Insert Size 2820 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004546.2, NP_004537.1
RefSeq Size 509 bp
RefSeq ORF 318 bp
Locus ID 4708
UniProt ID O95178
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Gene Summary The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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