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PCK2 (NM_004563) Human Untagged Clone
CAT＃: SC117281
PCK2 (untagged)-Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 4,776.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PEPCK; PEPCK-M; PEPCK2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117281 sequence for NM_004563 edited (data generated by NextGen Sequencing)
ATGGCCGCATTGTACCGCCCTGGCCTGCGGCTTAACTGGCATGGGCTGAGCCCCTTGGGC TGGCCATCATGCCGTAGCATCCAGACCCTGCGAGTGCTTAGTGGAGATCTGGGCCAGCTT CCCACTGGCATTCGAGATTTTGTAGAGCACAGTGCCCGCCTGTGCCAACCAGAGGGCATC CACATCTGTGATGGAACTGAGGCTGAGAATACTGCCACACTGACCCTGCTGGAGCAGCAG GGCCTCATCCGAAAGCTCCCCAAGTACAATAACTGCTGGCTGGCCCGCACAGACCCCAAG GATGTGGCACGAGTAGAGAGCAAGACGGTGATTGTAACTCCTTCTCAGCGGGACACGGTA CCACTCCCGCCTGGTGGGGCCCGTGGGCAGCTGGGCAACTGGATGTCCCCAGCTGATTTC CAGCGAGCTGTGGATGAGAGGTTTCCAGGCTGCATGCAGGGCCGCACCATGTATGTGCTT CCATTCAGCATGGGTCCTGTGGGCTCCCCGCTGTCCCGCATCGGGGTGCAGCTCACTGAC TCAGCCTATGTGGTGGCAAGCATGCGTATTATGACCCGACTGGGGACACCTGTGCTTCAG GCCCTGGGAGATGGTGACTTTGTCAAGTGTCTGCACTCCGTGGGCCAGCCCCTGACAGGA CAAGGGGAGCCAGTGAGCCAGTGGCCGTGCAACCCAGAGAAAACCCTGATTGGCCACGTG CCCGACCAGCGGGAGATCATCTCCTTCGGCAGCGGCTATGGTGGCAACTCCCTGCTGGGC AAGAAGTGCTTTGCCCTACGCATCGCCTCTCGGCTGGCCCGGGATGAGGGCTGGCTGGCA GAGCACATGCTGATCCTGGGCATCACCAGCCCTGCAGGGAAGAAGCGCTATGTGGCAGCC GCCTTCCCTAGTGCCTGTGGCAAGACCAACCTGGCTATGATGCGGCCTGCACTGCCAGGC TGGAAAGTGGAGTGTGTGGGGGATGATATTGCTTGGATGAGGTTTGACAGTGAAGGTCGA CTCCGGGCCATCAACCCTGAGAACGGCTTCTTTGGGGTTGCCCCTGGTACCTCTGCCACC ACCAATCCCAACGCCATGGCTACAATCCAGAGTAACACTATTTTTACCAATGTGGCTGAG ACCAGTGATGGTGGCGTGTACTGGGAGGGCATTGACCAGCCTCTTCCACCTGGTGTTACT GTGACCTCCTGGCTGGGCAAACCCTGGAAACCTGGTGACAAGGAGCCCTGTGCACATCCC AACTCTCGATTTTGTGCCCCGGCTCGCCAGTGCCCCATCATGGACCCAGCCTGGGAGGCC CCAGAGGGTGTCCCCATTGACGCCATCATCTTTGGTGGCCGCAGACCCAAAGGGGTACCC CTGGTATACGAGGCCTTCAACTGGCGTCATGGGGTGTTTGTGGGCAGCGCCATGCGCTCT GAGTCCACTGCTGCAGCAGAACACAAAGGGAAGATCATCATGCACGACCCATTTGCCATG CGGCCCTTTTTTGGCTACAACTTCGGGCACTACCTGGAACACTGGCTGAGCATGGAAGGG CGCAAGGGGGCCCAGCTGCCCCGTATCTTCCATGTCAACTGGTTCCGGCGTGACGAGGCA GGGCACTTCCTGTGGCCAGGCTTTGGGGAGAATGCTCGGGTGCTAGACTGGATCTGCCGG CGGTTAGAGGGGGAGGACAGTGCCCGAGAGACACCCATTGGGCTGGTGCCAAAGGAAGGA GCCTTGGATCTCAGCGGCCTCAGAGCTATAGACACCACTCAGCTGTTCTCCCTCCCCAAG GACTTCTGGGAACAGGAGGTTCGTGACATTCGGAGCTACCTGACAGAGCAGGTCAACCAG GATCTGCCCAAAGAGGTGTTGGCTGAGCTTGAGGCCCTGGAGAGACGTGTGCACAAAATG TGA Clone variation with respect to NM_004563.2 362 a=>c >OriGene 5' read for NM_004563 unedited
CCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGGCTCCGCT CGGTTCCTGGCCACCCCGCAGCCCCTGCCCAGGTGCCATGGCCGCATTGTACCGCCCTGG CCTGCGGCTTAACTGGCATGGGCTGAGCCCCTTGGGCTGGCCATCATGCCGTAGCATCCA GACCCTGCGAGTGCTTAGTGGAGATCTGGGCCAGCTTCCCACTGGCATTCGAGATTTTGT AGAGCACAGTGCCCGCCTGTGCCAACCAGAGGGCATCCACATCTGTGATGGAACTGAGGC TGAGAATACTGCCACACTGACCCTGCTGGAGCAGCAGGGCCTCATCCGAAAGCTCCCCAA GTACAATAACTGCTGGCTGGCCCGCACAGACCCCAAGGATGTGGCACGAGTAGAGAGCAA GACGGTGATTGTAACTCCTTCTCAGCGGGACACGGTACCACTCCCGCCTGGTGGGGCCCG TGGGCAGCTGGGCAACTGGATGTCCCCAGCTGATTTCCAGCGAGCTGTGGATGAGAGGTT TCCAGGCTGCATGCAGGGCCGCACCATGTATGTGCTTCCATTCAGCATGGGTCCTGTGGG CTCCCCGCTGTCCCGCATCGGGGTGCAGCTCACTGACTCAGCCTATGTGGTGGCAAGCAT GCGTATTATGACCCGACTGGGGACACCTGTGCTTCAGGCCCTGNGAGATGGTGACTNTGT CAAGTGTCTGCACTCCGTGGGCCAGCCCCTGACAGGACAAGGNGAGCCAGTGAGCCAGTG GCCGTGCCACCCAGAGAAACCCTGATTGGTCACGTGCCCGACCAGCGGGAGATCATCTCC CTCGGCAGCGGCTATGGTGGGCACTCCCTGCTGGGNCAGAAGTGCTTTGCCCTACGCATC GNCTCTCGGCTGCCCCGGNATGAGGNNNCTGCTGCANACACATGCTGATCCTGGCATCAC AGACTGCAAGG >OriGene 3' read for NM_004563 unedited
GACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTGTAAAATAGATAAG CATCTCTTATTATTGGACAGTCTTTGTGGGAAGGTCTATGGCACATTGAAGATGTTAGTT AATATCAAATTGCTCATATTTTCTGCAAGGTGCCTTCCCTATTCCCAGATGAGGGTGCTA TGTCCTCTTGCTAGACTAGGGCCTCAGGTCACATTTTGTGCACACGTCTCTCCAGGGCCT CAAGCTCAGCCAACACCTCTTTGGGCAGATCCTGGTTGACCTGCTCTGTCAGGTAGCTCC GAATGTCACGAACCTCCTGTTCCCAGAAGTCCTTGGGGAGGGAGAACAGCTGAGTGGTGT CTATAGCTCTGAGGCCGCTGAGATCCAAGGCTCCTTCCTTTGGCACCAGCCCAATGGGTG TCTCTCGGGCACTGTCCTCCCCCTCTAACCGCCGGCAGATCCAGTCTAGCACCCGAGCAT TCTCCCCAAAGCCTGGCCACAGGAAGTGCCCTGCCTCGTCACGCCGGAACCAGTTGACAT GGAAGATACGGGGCAGCTGGGCCCCCTTGCGCCCTTCCATGCTCAGCCAGTGTTCCAGGT AGTGCCCGAAGTTGTAGCCAAAAAAGGGCCGCATGGCAAATGGGTCGTGCATGATGATCT TCCCTTTGTGTTCTGCTGCAGCAGTGGACTCAGAGCGCATGGCGCTGCCCACAAACACCC CATGACGCCAGTTGAAGGCCTCGTATACCAGGGGTACCCCCTTTGGGTCTGCGGCCACCA AGATGATGGCGTCAATGGGGACACCCCCTCTGGGCCTNCCCAGCCTGGTCCATGATGGNG GCACTGGCGAGCCCGNGCACAAATCGAGAGTTGGATGTGACAGGGCTCCTTGTACCAGTT NCCAGGTTGCCCAGCAGNAGTACAGTACACCAGNTGGAAAGCTGTCATGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004563 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004563.2, NP_004554.2 |
| RefSeq Size | 2221 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 5106 |
| UniProt ID | Q16822 |
| Domains | PEPCK |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Adipocytokine signaling pathway, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism |
| Gene Summary | This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200354 | PCK2 (Myc-DDK-tagged)-Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,768.00 |
|
| RC200354L1 | Lenti ORF clone of Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 7,168.00 |
|
| RC200354L2 | Lenti ORF clone of Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200354L3 | Lenti ORF clone of Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200354L4 | Lenti ORF clone of Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 7,168.00 |
|
| RG200354 | PCK2 (tGFP-tagged) - Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6,368.00 |
|
| SC320611 | PCK2 (untagged)-Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,776.00 |
