United States
Germany
Japan
United Kingdom
China
LARGE (LARGE1) (NM_004737) Human Untagged Clone
CAT＃: SC117185
LARGE (untagged)-Human like-glycosyltransferase (LARGE), transcript variant 1
CNY 5,544.00
CNY 7,220.00
货期*
现货
规格
| Cited in 2 publications. |
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LARGE; MDC1D; MDDGA6; MDDGB6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004737, the custom clone sequence may differ by one or more nucleotides
ATGCTGGGAATCTGCAGGGGGAGACGGAAATTCTTGGCTGCCTCGTTGAGTCTTCTCTGCATCCCAGCCA TCACCTGGATTTACCTGTTTTCTGGGAGCTTCGAAGATGGAAAGCCCGTGTCTCTGTCACCGCTGGAGTC CCAGGCACACAGCCCCAGGTACACGGCCTCCAGCCAGCGGGAGCGCGAGAGCCTGGAGGTGCGCATGCGC GAGGTGGAGGAGGAGAACCGCGCCCTCCGCAGGCAGCTCAGCCTGGCCCAGGGCCGAGCCCCATCCCATC GCCGAGGCAACCACTCCAAGACCTACTCCATGGAGGAGGGCACTGGAGACAGCGAGAACCTTCGGGCTGG CATCGTGGCAGGCAACAGCTCCGAGTGTGGGCAGCAGCCGGTCGTGGAGAAATGCGAGACAATCCACGTT GCTATTGTCTGCGCCGGATACAATGCCAGCCGGGATGTCGTCACCCTGGTCAAATCCGTCCTGTTCCATA GACGGAACCCTCTGCACTTCCACCTTATTGCTGACTCCATTGCGGAGCAGATCCTGGCCACGCTCTTCCA GACCTGGATGGTGCCCGCTGTGCGTGTGGACTTCTACAATGCAGACGAGCTCAAGTCTGAAGTTTCCTGG ATCCCCAATAAACATTACTCTGGGATTTATGGTCTGATGAAGCTTGTCCTGACCAAGACTCTTCCTGCCA ACCTGGAGAGAGTCATCGTCCTTGACACGGATATCACCTTTGCCACTGACATTGCAGAGCTGTGGGCTGT GTTCCACAAGTTCAAAGGTCAGCAAGTCCTGGGCTTGGTGGAGAACCAGAGTGACTGGTACCTTGGAAAC CTGTGGAAAAATCACCGCCCATGGCCAGCCCTTGGAAGAGGCTACAACACAGGGGTGATCCTGTTACTTC TGGATAAGCTGCGGAAGATGAAATGGGAGCAGATGTGGAGGCTGACCGCAGAGAGGGAGCTCATGGGCAT GCTCTCTACATCCTTAGCTGACCAGGATATTTTCAATGCCGTCATCAAACAAAACCCCTTCCTTGTGTAC CAGCTCCCCTGCTTCTGGAATGTGCAGCTGTCAGACCACACCCGCTCCGAGCAGTGCTACAGAGACGTGT CTGATCTAAAGGTCATTCACTGGAACTCCCCCAAGAAGCTCCGGGTGAAGAACAAGCATGTGGAGTTTTT TCGCAACCTCTACCTGACCTTCCTGGAGTATGACGGCAATCTTCTGAGGCGGGAACTGTTTGGCTGCCCC AGTGAGGCTGATGTCAACAGTGAAAACCTCCAGAAGCAGCTGTCTGAGCTGGACGAGGACGACCTGTGCT ATGAGTTCCGGCGAGAGCGCTTCACTGTCCACCGCACCCACCTGTACTTCCTGCACTACGAGTATGAGCC TGCAGCAGACAGCACGGACGTCACCCTGGTCGCTCAGCTGTCCATGGACAGGCTCCAGATGCTGGAGGCC ATCTGCAAGCACTGGGAGGGGCCCATCAGCCTGGCCCTCTACCTGTCAGACGCCGAGGCCCAGCAGTTCC TCCGCTACGCACAGGGCTCTGAGGTGCTTATGAGCCGCCACAACGTGGGCTACCACATCGTGTACAAGGA GGGCCAGTTCTACCCCGTGAACCTGCTGCGCAACGTGGCCATGAAGCACATCAGCACTCCCTACATGTTC CTGTCTGACATTGACTTCCTGCCCATGTATGGGCTCTATGAGTACCTCAGGAAGTCTGTCATCCAGCTCG ATCTTGCCAACACCAAGAAAGCAATGATTGTCCCCGCGTTCGAGACACTGCGCTACCGGCTGTCCTTCCC CAAGTCAAAAGCGGAGTTGCTGTCAATGCTGGACATGGGGACCCTCTTCACATTCAGGTACCACGTCTGG ACGAAAGGCCACGCACCCACAAACTTCGCCAAGTGGCGGACCGCCACCACGCCTTACCGGGTTGAGTGGG AGGCCGATTTTGAGCCGTATGTTGTTGTGAGACGTGACTGCCCGGAGTACGACCGGAGGTTTGTAGGCTT TGGCTGGAACAAAGTGGCTCATATCATGGAGCTGGATGTGCAGGAGTATGAGTTCATTGTGCTGCCCAAC GCCTACATGATCCACATGCCTCATGCCCCCAGCTTCGACATTACCAAGTTCCGTTCCAACAAGCAATACC GCATCTGTCTCAAAACCCTCAAGGAAGAGTTTCAGCAGGACATGTCCCGCCGCTACGGCTTTGCTGCCCT GAAATATCTCACAGCCGAGAACAACAGCTAG >OriGene 5' read for NM_004737 unedited
NGGTCCAAATTTTGNTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGCTGG CTCGGAAGAGTCCCCCGGGCCGGGAGCAGCTCCAGGCAGCGGCCCCGGAGGAAGAGGAAG AAGGGACAGTGCTCAGCTTGGGGGACCCGGACCCTCGCCGCGGCATTTGGAGCCGGGGGC AGTCCCGAACTCTGTGCTTGGCACCGCCGCTCCGAGTAGGGCAGCGCCTGCCGGGACCCT GACCCGGACCCCCTGCGCCTCGTAGGCGGCGGCGCCGCCGCGCCACCCTGTTCTTCCGTG TCTCCCTCTGCCTGGCGGCAGTCACGGCCAAGAGAGTATTATGAGGGAGGCCGAGGACTT CATGCTCCGGACAGAGAAACGGCGCTGGGATTAGGGATTGCCACTTCTGAGAGGATGCTG GGAATCTGCAGGGGGAGACGGAAATTCTTGGCTGCCTCGTTGAGTCTTCTCTGCATCCCA GCCATCACCTGGATTTACCTGTTTTCTGGGAGCTTCGAAGATGGAAAGCCCGTGTCTCTG TCACCGCTGGAGTCCCAGGCACACAGCCCCAGGTACACGGCCTCCAGCCAGCGGGAGCGC GAGAGCCTGGAGGTGCGCATGCGCGAGGTGGAGGAGGAGAACCGCGCCCTCCGCAGGCAG CTCAGCCTGGCCCAGGGCCGAGCCCCATCCCATCGCCGAGGCAACCACTCCAAGACCTAC TCCATGGAGGANGGCACTGGAGACAGCGAGAACCTTCGGGCTGGCATCGTGGCAGGCAAC AGCTCCGAGTGTGGGCAGCAGCCCGNTCGTGGAGAAATGCGAGACAAATCCACGTGCTAT TGTCTGCGCTGGATCAATGCCAGCCGGGATGTCGTCACCTGGTCAATCCCGTCTGTCCAT AGACGNA >OriGene 3' read for NM_004737 unedited
NNNAAAATTTAGCTATGNCCGCGGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTG TCTGTACTTTCATTTATTAAATGCAGCTTTCGCTGGTCACATTGCAACTCAATACAAACA GCCCAGCAGAGGGTAGGGCATTAACTCTTGTTCTCATCAATGTAAACACAATATTAATAT TGAAGATTAAAGAAAATGGGTAAAAAGTCCAGTGTCTCCTCAATCTGTGTGAAGGTGAAG GTGGTTGCCTACCCTTGGACAGGTCCCAAAGGGAGACTGAGGTTGTCACCTCTTCCCATC CACAGTGAAGCAGGCAAGAACCAGAGGAACCCAATCACTTTCTTCCTGTAGGTCTCCAGA TGGATACGTGAATCTTCAGAACTGGGCTTTCATGAATTATGAAAGTTCTTCGAAAGGCCT GAGAGGTTCGTACCTTGTGTGTGCACTGAAGTAGTTCCTTCAAAGCCCTTTCCCATGAAT CAGTCACTGCCAATANAAAATGTGACCGGTGTAAAACAGCCAGCCCTCGTAATTCCGCAG TCCCCATCGCTATAGCCCCTGGATTCTGGAGAGTAGGGAGTTCAAAGTCACGGGAGCCTC CGTGATCATCCTGAAGGAAGCTGCCCATGTTAAATATCGGGCGAAGATGCTTGACCTCCT TCCTGGGCCACTGCTGAATGAGGNGGGGTTGGTAAAAGCAANNTAATTTTTCTTTATAAC CCTCTAAGATCAGATTTTTTATTTTGGATTGGCACCCCCAAAATAAACAAACCCCCCAGA AAAAACAAACAAAACAGGAGTGACTTTTGGGATAAAGGAAACCATTACCCCCCCTCCCGG AACCCTGGGTTCAAGGCCCCATTGGGTTCTTCCAAAAGGGGGGGTTAAAAAATAAGTATG CCCTCCGTTGGAAACCCAACTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004737 |
| Insert Size | 4110 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004737.3, NP_004728.1 |
| RefSeq Size | 4194 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 9215 |
| UniProt ID | O95461 |
| Domains | Glyco_transf_8 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. The protein encoded by this gene is the glycotransferase that adds the final xylose and glucuronic acid to alpha-dystroglycan and thereby allows alpha-dystroglycan to bind ligands including laminin 211 and neurexin. Mutations in this gene cause several forms of congenital muscular dystrophy characterized by cognitive disability and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2018] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dystroglycan Binding to a-Neurexin Competes with Neurexophilin-1 and Neuroligin in the Brain
,Reissner, C;Stahn, J;Breuer, D;Klose, M;Pohlentz, G;Mormann, M;Missler, M;,
J. Biol. Chem.
,PubMed ID 25157101
[LARGE1]
|
|
Overexpression of LARGE suppresses muscle regeneration via down-regulation of insulin-like growth factor 1 and aggravates muscular dystrophy in mice
,Saito, F;Kanagawa, M;Ikeda, M;Hagiwara, H;Masaki, T;Ohkuma, H;Shimizu, T;Sonoo, M;Toda, T;Matsumura, K;,
Hum. Mol. Genet. April 2014
,PubMed ID 24722207
[LARGE1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217876 | LARGE (Myc-DDK-tagged)-Human like-glycosyltransferase (LARGE), transcript variant 1 |
CNY 5,536.00 |
|
| RC217876L3 | Lenti ORF clone of Human like-glycosyltransferase (LARGE), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217876L4 | Lenti ORF clone of Human like-glycosyltransferase (LARGE), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217876 | LARGE (tGFP-tagged) - Human like-glycosyltransferase (LARGE), transcript variant 1 |
CNY 4,370.00 |
Customer
Reviews
Loading...
