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PERK (EIF2AK3) (NM_004836) Human Untagged Clone
CAT＃: SC117132
EIF2AK3 (untagged)-Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3)
CNY 11,664.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PEK; PERK; WRS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_004836 edited
ATGGAGCGCGCCATCAGCCCGGGGCTGCTGGTACGGGCGCTGCTGCTGCTGCTGCTGCTG CTGGGGCTCGCGGCAAGGACGGTGGCCGCGGGGCGCGCCCGTGGCCTCCCAGCGCCGACG GCGGAGGCGGCGTTCGGCCTCGGGGCGGCCGCTGCTCCCACCTCAGCGACGCGAGTACCG GCGGCGGGCGCCGTGGCTGCGGCCGAGGTGACTGTGGAGGACGCTGAGGCGCTGCCGGCA GCCGCGGGAGAGCAGGAGCCTCGGGGTCCGGAACCAGACGATGAGACAGAGTTGCGACCG CGCGGCAGGTCATTAGTAATTATCAGCACTTTAGATGGGAGAATTGCTGCCTTGGATCCT GAAAATCATGGTAAAAAGCAGTGGGATTTGGATGTGGGATCCGGTTCCTTGGTGTCATCC AGCCTTAGCAAACCAGAGGTATTTGGGAATAAGATGATCATTCCTTCCCTGGATGGAGCC CTCTTCCAGTGGGACCGAGACCGTGAAAGCATGGAAACAGTTCCTTTCACAGTTGAATCA CTTCTTGAATCTTCTTATAAATTTGGAGATGATGTTGTTTTGGTTGGAGGAAAATCTCTG ACTACATATGGACTCAGTGCATATAGTGGAAAGGTGAGGTATATCTGTTCAGCTCTGGGT TGTCGCCAATGGGATAGTGACGAAATGGAACAAGAGGAAGACATCCTGCTTCTACAGCGT ACCCAAAAAACTGTTAGAGCTGTCGGACCTCGCAGTGGCAATGAGAAGTGGAATTTCAGT GTTGGCCACTTTGAACTTCGGTATATTCCAGACATGGAAACGAGAGCCGGATTTATTGAA AGCACCTTTAAGCCCAATGAGAACACAGAAGAGTCTAAAATTATTTCAGATGTGGAAGAA CAGGAAGCTGCCATAATGGACATAGTGATAAAGGTTTCGGTTGCTGACTGGAAAGTTATG GCATTCAGTAAGAAGGGAGGACATCTGGAATGGGAGTACCAGTTTTGTACTCCAATTGCA TCTGCCTGGTTACTTAAGGATGGGAAAGTCATTCCCATCAGTCTTTTTGATGATACAAGT TATACATCTAATGATGATGTTTTAGAAGATGAAGAAGACATTGTAGAAGCTGCCAGAGGA GCCACAGAAAACAGTGTTTACTTGGGAATGTATAGAGGCCAGCTGTATCTGCAGTCATCA GTCAGAATTTCAGAAAAGTTTCCTTCAAGTCCCAAGGCTTTGGAATCTGTCACTAATGAA AACGCAATTATTCCTTTACCAACAATCAAATGGAAACCCTTAATTCATTCTCCTTCCAGA ACTCCTGTCTTGGTAGGATCTGATGAATTTGACAAATGTCTCAGTAATGATAAGTTTTCT CATGAAGAATATAGTAATGGTGCACTTTCAATCTTGCAGTATCCATATGATAATGGTTAT TATCTACCATACTACAAGAGGGAGAGGAACAAACGAAGCACACAGATTACAGTCAGATTC CTCGACAACCCACATTACAACAAGAATATCCGCAAAAAGGATCCTGTTCTTCTTTTACAC TGGTGGAAAGAAATAGTTGCAACGATTTTGTTTTGTATCATAGCAACAACGTTTATTGTG CGCAGGCTTTTCCATCCTCATCCTCACAGGCAAAGGAAGGAGTCTGAAACTCAGTGTCAA ACTGAAAATAAATATGATTCTGTAAGTGGTGAAGCCAATGACAGTAGCTGGAATGACATA AAAAACTCTGGATATATATCACGATATCTAACTGATTTTGAGCCAATTCAATGCCTGGGA CGTGGTGGCTTTGGAGTTGTTTTTGAAGCTAAAAACAAAGTAGATGACTGCAATTATGCT ATCAAGAGGATCCGTCTCCCCAATAGGGAATTGGCTCGGGAAAAGGTAATGCGAGAAGTT AAAGCCTTAGCCAAGCTTGAACACCCGGGCATTGTTAGATATTTCAATGCCTGGCTCGAA GCACCACCAGAGAAGTGGCAAGAAAAGATGGATGAAATTTGGCTGAAAGATGAAAGCACA GACTGGCCACTCAGCTCTCCTAGCCCAATGGATGCACCATCAGTTAAAATACGCAGAATG GATCCTTTCTCTACAAAAGAACATATTGAAATCATAGCTCCTTCACCACAAAGAAGCAGG TCTTTTTCAGTAGGGATTTCCTGTGACCAGACAAGTTCATCTGAGAGCCAGTTCTCACCA CTGGAATTCTCAGGAATGGACCATGAGGACATCAGTGAGTCAGTGGATGCAGCATACAAC CTCCAGGACAGTTGCCTTACAGACTGTGATGTGGAAGATGGGACTATGGATGGCAATGAT GAGGGGCACTCCTTTGAACTTTGTCCTTCTGAAGCTTCTCCTTATGTAAGGTCAAGGGAG AGAACCTCCTCTTCAATAGTATTTGAAGATTCTGGCTGTGATAATGCTTCCAGTAAAGAA GAGCCGAAAACTAATCGATTGCATATTGGCAACCATTGTGCTAATAAACTAACTGCTTTC AAGCCCACCAGTAGCAAATCTTCTTCTGAAGCTACATTGTCTATTTCTCCTCCAAGACCA ACCACTTTAAGTTTAGATCTCACTAAAAACACCACAGAAAAACTCCAGCCCAGTTCACCA AAGGTGTATCTTTACATTCAAATGCAGCTGTGCAGAAAAGAAAACCTCAAAGACTGGATG AATGGACGATGTACCATAGAGGAGAGAGAGAGGAGCGTGTGTCTGCACATCTTCCTGCAG ATCGCAGAGGCAGTGGAGTTTCTTCACAGTAAAGGACTGATGCACAGGGACCTCAAGCCA TCCAACATATTCTTTACAATGGATGATGTGGTCAAGGTTGGAGACTTTGGGTTAGTGACT GCAATGGACCAGGATGAGGAAGAGCAGACGGTTCTGACCCCAATGCCAGCTTATGCCAGA CACACAGGACAAGTAGGGACCAAACTGTATATGAGCCCAGAGCAGATTCATGGAAACAGC TATTCTCATAAAGTGGACATCTTTTCTTTAGGCCTGATTCTATTTGAATTGCTGTATCCA TTCAGCACTCAGATGGAGAGAGTCAGGACCTTAACTGATGTAAGAAATCTCAAATTTCCA CCATTATTTACTCAGAAATATCCTTGTGAGTACGTGATGGTTCAAGACATGCTCTCTCCA TCCCCCATGGAACGACCTGAAGCTATAAACATCATTGAAAATGCTGTATTTGAGGACTTG GACTTTCCAGGAAAAACAGTGCTCAGACAGAGGTCTCGCTCCTTGAGTTCATCGGGAACA AAACATTCAAGACAGTCCAACAACTCCCATAGCCCTTTGCCAAGCAATTAG >OriGene 5' read for NM_004836 unedited
GCAGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCCGGGCT GAGACGTGGCCAGGGGAACACGGCTGGCTGTCCAGGCCGTCGGGGCGGCAGTAGGGTCCC TAGCACGTCCTTGCCTTCTTGGGAGCTCCAAGCGGCGGGAGAGGCAGGCGTCAGTGGCTG CGCCTCCATGCCTGCGCGCGGGGCGGGACGCTGATGGAGCGCGCCATCAGCCCGGGGCTG CTGGTACGGGCGCTGCTGCTGCTGCTGCTGCTGCTGGGGCTCGCGGCAAGGACGGTGGCC GCGGGGCGCGCCCGTGGCCTCCCAGCGCCGACGGCGGAGGCGGCGTTCGGCCTCGGGGCG GCCGCTGCTCCCACCTCAGCGACGCGAGTACCGGCGGCGGGCGCCGTGGCTGCGGCCGAG GTGACTGTGGAGGACGCTGAGGCGCTGCCGGCAGCCGCGGGAGAGCAGGAGCCTCGGGGT CCGGAACCAGACGATGAGACAGAGTTGCGACCGCGCGGCAGGTCATTAGTAATTATCAGC ACTTTAGATGGGAGAATTGCTGCCTTGGATCCTGAAAATCATGGTAAAAAGCAGTGGGAT TTGGATGTGGGATCCGGTTCCTTGGTGTCATCCAGCCTTAGCAAACCAGAGGTATTTGGG AATAAGATGATCATTCCTTCCCTGGATGGAGCCCTCTTCCAGTGGGACCGAGACCGTGAA AGCATGGAAACAGTTCCTTTCACAGTTGAATCACTTCTTGAATCTTCTTTATAATTTGGA GATGATGTTGTTTTGGTTGGGAGGAAATCTCTGACTACATATGGACTCAGTGCATATAGT GGAAAGGTGAGGTATATCTGTTCAGCTCTGGGGTGTCGCCATGGGATAGTGACGAATGGG ACCAGAGGAAGACTNNCTGCTCTACGCGTACCCAANAACTGTTAC >OriGene 3' read for NM_004836 unedited
GTGGACGAAGCCGCCTGNNAGATCGAGTTTTTTTCTTTTTTTTTTTTTTAATTAAAATTA GTTTTATTAAATCATTTAAACTTGAAAAAATCACAAGTTAACACACTTAAGTGTGTTCTG TACACCACCAACCCAAATGGATTGATTCCACAATTTTTATAAATAAAACATAAACATATT TACAGTGAAAAATAAAAACATGTCAGCAAGCGATATGATTAAACTGTTACCTATAATAAA TTTGGGGGAAGTACCAACCTACCGGATTACGTGTCCTATACTTACCATTCCATATCGTTT CCCAATCCCCTCCGACCCTAAACCCCCCCCCCCCAGTACGTAGGCATCCCTTACTCCCAA TTCTCTTCATCCAATCTTTATTCCCCTCACATCTCCAATCAACACTCTATTCTCTTCCCA TCTTACCCCCACTTTTCTTCTTTTGTTTTCATTTCTTTCTAGTCTACCCCCCCTCCTACT CCCTCCCACCCCTCTTCATCTTTCCTTCTTCCAATCCCCTCCGTGTTTTTCCCCCTTCCC CTTTTAATTCTTTCCACCTTTTTCTTCCCCCTCTCCGCCCCCATTTTTAACTCCCCTATT TTTCCCTCCTTTTACACCCCCCCTGTTTTTCCAGCCCCACCCCCCCCTTTTTTTTTCTAT TTTTTATCCCACCCCCCTCCTATCCCCCCTTTTTCCCTTTCTCCCTATCCCCCCCCATTT TTATTTTTTCTCCTTCCCCCCCCCCTTTTTTTCCTTCCCTTCCCCCCTCCTCCTTCTCCC CTACTATTTCTTATATCCTCCACCCCCTTTCTCTCTTCCCCCCCCCCCACCCCTCCCCTT TTTTTCCCCCTCCCCCCCTTTCCCCATTCCCCCCCACCCTTCCCGTCCTCTTACCTAATA TCATCACACATTCCCCCACCGCTCACCCCCCCCCTTTTTTGTTTTTCCTCCCCCCTTTCA CCCCCCCGCCTCTATTTCACCCACCTCACTACCTAATTTAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004836 |
| Insert Size | 4280 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004836.3, NP_004827.3 |
| RefSeq Size | 4511 bp |
| RefSeq ORF | 3351 bp |
| Locus ID | 9451 |
| UniProt ID | Q9NZJ5 |
| Domains | pkinase, TyrKc, S_TKc, PQQ |
| Protein Families | Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane |
| Protein Pathways | Alzheimer's disease |
| Gene Summary | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ER stress potentiates insulin resistance through PERK-mediated FOXO phosphorylation
,Wei Zhang, Ville Hietakangas, Sheena Wee, Siew Choo Lim, Jayantha Gunaratne, and Stephen M. Cohen,
Genes & Dev., Feb 2013; 27: 441 - 449.
[EIF2AK3]
|
|
ER stress potentiates insulin resistance through PERK-mediated FOXO phosphorylation
,null,
Genes & Development
,PubMed ID 23431056
[EIF2AK3]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214993 | EIF2AK3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) |
CNY 11,656.00 |
|
| RC214993L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), Myc-DDK-tagged |
CNY 14,056.00 |
|
| RC214993L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), mGFP tagged |
CNY 13,020.00 |
|
| RC214993L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), Myc-DDK-tagged |
CNY 13,020.00 |
|
| RC214993L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), mGFP tagged |
CNY 14,056.00 |
|
| RG214993 | EIF2AK3 (tGFP-tagged) - Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) |
CNY 13,256.00 |
|
| SC323503 | EIF2AK3 (untagged)-Kinase deficient mutant (K622M) of Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) |
CNY 11,664.00 |
