ENT1 (SLC29A1) (NM_004955) Human Untagged Clone

CAT#: SC117050

SLC29A1 (untagged)-Human solute carrier family 29 (nucleoside transporters), member 1 (SLC29A1), nuclear gene encoding mitochondrial protein, transcript variant 5



  "NM_004955" in other vectors (4)

CNY 3,656.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
Rabbit Polyclonal Antibody against SLC29A1 (Center)
    • 200 ul

CNY 6,314.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ENT1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004955, the custom clone sequence may differ by one or more nucleotides


ATGACAACCAGTCACCAGCCTCAGGACAGATACAAAGCTGTCTGGCTTATCTTCTTCATGCTGGGTCTGG
GAACGCTGCTCCCGTGGAATTTTTTCATGACGGCCACTCAGTATTTCACAAACCGCCTGGACATGTCCCA
GAATGTGTCCTTGGTCACTGCTGAACTGAGCAAGGACGCCCAGGCGTCAGCCGCCCCTGCAGCACCCTTG
CCTGAGCGGAACTCTCTCAGTGCCATCTTCAACAATGTCATGACCCTATGTGCCATGCTGCCCCTGCTGT
TATTCACCTACCTCAACTCCTTCCTGCATCAGAGGATCCCCCAGTCCGTACGGATCCTGGGCAGCCTGGT
GGCCATCCTGCTGGTGTTTCTGATCACTGCCATCCTGGTGAAGGTGCAGCTGGATGCTCTGCCCTTCTTT
GTCATCACCATGATCAAGATCGTGCTCATTAATTCATTTGGTGCCATCCTGCAGGGCAGCCTGTTTGGTC
TGGCTGGCCTTCTGCCTGCCAGCTACACGGCCCCCATCATGAGTGGCCAGGGCCTAGCAGGCTTCTTTGC
CTCCGTGGCCATGATCTGCGCTATTGCCAGTGGCTCGGAGCTATCAGAAAGTGCCTTCGGCTACTTTATC
ACAGCCTGTGCTGTTATCATTTTGACCATCATCTGTTACCTGGGCCTGCCCCGCCTGGAATTCTACCGCT
ACTACCAGCAGCTCAAGCTTGAAGGACCCGGGGAGCAGGAGACCAAGTTGGACCTCATTAGCAAAGGAGA
GGAGCCAAGAGCAGGCAAAGAGGAATCTGGAGTTTCAGTCTCCAACTCTCAGCCCACCAATGAAAGCCAC
TCTATCAAAGCCATCCTGAAAAATATCTCAGTCCTGGCTTTCTCTGTCTGCTTCATCTTCACTATCACCA
TTGGGATGTTTCCAGCCGTGACTGTTGAGGTCAAGTCCAGCATCGCAGGCAGCAGCACCTGGGAACGTTA
CTTCATTCCTGTGTCCTGTTTCTTGACTTTCAATATCTTTGACTGGTTGGGCCGGAGCCTCACAGCTGTA
TTCATGTGGCCTGGGAAGGACAGCCGCTGGCTGCCAAGCCTGGTGCTGGCCCGGCTGGTGTTTGTGCCAC
TGCTGCTGCTGTGCAACATTAAGCCCCGCCGCTACCTGACTGTGGTCTTCGAGCACGATGCCTGGTTCAT
CTTCTTCATGGCTGCCTTTGCCTTCTCCAACGGCTACCTCGCCAGCCTCTGCATGTGCTTCGGGCCCAAG
AAAGTGAAGCCAGCTGAGGCAGAGACCGCAGGAGCCATCATGGCCTTCTTCCTGTGTCTGGGTCTGGCAC
TGGGGGCTGTTTTCTCCTTCCTGTTCCGGGCAATTGTGTGA


>OriGene 5' read for NM_004955 unedited
GCACGAGGCGGGAGAGGGAAGCTGCAGCGAGAGCGCGCGGATCTCAGCGCGGGAGCAGTG
CTTCTGCGGCAGGCCCCTGAGGGAGGGAGCTGTCAGCCAGGGAAAACCGAGAACACCATC
ACCATGACAACCAGTCACCAGCCTCAGGACAGATACAAAGCTGTCTGGCTTATCTTCTTC
ATGCTGGGTCTGGGAACGCTGCTCCCGTGGAATTTTTTCATGACGGCCACTCAGTATTTC
ACAAACCGCCTGGACATGTCCCAGAATGTGTCCTTGGTCACTGCTGAACTGAGCAAGGAC
GCCCAGGCGTCAGCCGCCCCTGCAGCACCCTTGCCTGAGCGGAACTCTCTCAGTGCCATC
TTCAACAATGTCATGACCCTATGTGCCATGCTGCCCCTGCTGTTATTCACCTACCTCAAC
TCCTTCCTGCATCAGAGGATCCCCCAGTCCGTACGGATCCTGGGCAGCCTGGTGGCCATC
CTGCTGGTGTTTCTGATCACTGCCATCCTGGTGAAGGTGCAGCTGGATGCTCTGCCCTTC
TTTGTCATCACCATGATCAAGATCGTGCTCATTAATTCATTTGGTGCCATCCTG
>OriGene 3' read for NM_004955 unedited
GTACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGAAAAAAACAAGNA
AGGTTTATTTAAATCAGAGAGCCTCTGAAGGCACCTGGTTTCTGTCAAATATATTTTTAA
AAAAGGCAGTAACGTGGCAACTGTACAGGGTGCATGATGGGTAAGAACTGGGCATGGGGA
GTTGGGAGGGGGACATGGAGAGAACACAGAGGAGGAGGCGCAGGGGAGAATGGAGTATAT
CAGGTCAAACCATACAACGGTCAGACCCAGCTCCTAGCCACCCCAGGACAGGCAGACAGT
CTGACAGACACGGACGCAGACACACAGACACATACACAGAGACACACGTGTTCTCCAAGC
CCAAGAGCCTCCACTGGCCTGCACAAGCAGGGATCAGTCAGAGCCAAGAGTGGAGAAACT
CAAGCAAATGCCGAGGCTACACCCCGTCCCTCGACTCTGAGCCCCAAACCCACAATGTCC
CCACTGTCCGTCCAGAGGCTCCCTCCCCAGGGCCTGGAGATCCGGGCCCAGCACACGCCG
TGGAAAGCAGAAGTCAGTTAGAAGAAAAAACCGCCAGACCACTCAGGATCACCCCTGGCA
GAAGGAAGGGGCAGGAGGCAGACAGGGAGGGAGGCAGGCAGTCCTTCTGTCCATCCTTTG
TCACACAATTGCCCGGAACAGGAATGAGAAAACAGCCCCCAGTGCCAGACCCAGACACAG
GAAGAAAGCCATGATGGCTCCTGCGGTCTCTGCCTCAGCTGCTTCACTTTCTTGGGCCCG
AAACACATGCTTAGCTTGCGATGTATCCGTTTTAGAATGCAAAGCCACCATGAAGAATAT
GACCAGGCTCGTGCTCGAGACCACATCAGTAACGGGGGGCTTAATGTTGACACTCAGATT
GGACAACCCAGTCGGCCATACAG
Restriction Sites NotI-NotI     
ACCN NM_004955
Insert Size 2200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004955.1, NP_004946.1
RefSeq Size 2341 bp
RefSeq ORF 1371 bp
Locus ID 2030
Domains Nucleoside_tran
Protein Families Transmembrane
Gene Summary This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. This variant has an upstream in-frame AUG at nt 255-257. This AUG is not annotated because it has a weak Kozak signal and N-terminal sequencing (PMID 8986748) identified the downstream annotated AUG as the translation start. Variants 1, 2, 3, 4, and 5 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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