DDX1 (NM_004939) Human Untagged Clone
CAT#: SC117041
DDX1 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1)
CNY 5,432.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DBP-RB; UKVH5d |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC117041 sequence for NM_004939 edited (data generated by NextGen Sequencing)
ATGGCGGCCTTCTCCGAGATGGGTGTAATGCCTGAGATTGCACAAGCTGTGGAAGAGATG GATTGGCTCCTCCCAACTGATATCCAGGCTGAATCTATCCCATTGATCTTAGGAGGAGGT GATGTACTTATGGCTGCAGAAACAGGAAGTGGCAAAACTGGTGCTTTTAGTATTCCAGTT ATCCAGATAGTTTATGAAACTCTGAAAGACCAACAGGAAGGCAAAAAAGGAAAAACAACA ATTAAAACTGGTGCTTCAGTGCTGAACAAATGGCAGATGAACCCATATGACAGAGGATCT GCTTTTGCAATTGGGTCAGATGGTCTTTGTTGTCAAAGCAGAGAAGTAAAGGAATGGCAT GGGTGTAGAGCTACTAAAGGATTAATGAAAGGGAAACACTACTATGAAGTATCCTGTCAT GACCAAGGGTTATGCAGGGTCGGGTGGTCTACCATGCAGGCCTCTTTGGACCTAGGTACT GACAAGTTTGGATTTGGCTTTGGTGGAACAGGAAAGAAATCCCATAACAAACAATTTGAT AATTATGGAGAGGAATTCACTATGCATGATACCATTGGATGTTACCTGGATATAGATAAG GGACATGTCAAGTTCTCCAAAAATGGAAAAGATCTTGGTCTGGCATTTGAAATACCACCA CATATGAAAAACCAAGCCCTCTTTCCTGCCTGTGTTTTGAAGAATGCTGAACTGAAATTT AACTTCGGTGAAGAGGAATTTAAGTTTCCACCAAAAGATGGCTTTGTTGCTCTTTCCAAG GCACCGGATGGTTACATTGTCAAATCACAGCACTCAGGTAATGCACAGGTGACACAAACA AAGTTTCTCCCCAATGCTCCGAAAGCTCTCATTGTTGAACCTTCCCGGGAGTTAGCTGAA CAAACTTTGAATAACATCAAGCAGTTTAAGAAATACATTGATAATCCTAAATTAAGGGAG CTTCTGATAATTGGAGGTGTTGCAGCCCGGGATCAGCTCTCTGTTTTGGAAAATGGAGTA GATATAGTTGTAGGTACTCCGGGAAGACTAGATGACTTGGTGTCAACTGGAAAGCTGAAC TTATCTCAAGTTAGATTCCTGGTCCTGGATGAAGCTGATGGGCTTCTTTCTCAAGGTTAT TCTGATTTTATAAATAGGATGCACAATCAGATTCCTCAGGTTACCTCTGATGGAAAAAGA CTTCAGGTGATTGTTTGCTCTGCCACTTTGCATTCTTTCGATGTAAAGAAACTGTCCGAG AAGATAATGCATTTTCCTACATGGGTTGACTTAAAAGGAGAAGACTCTGTTCCAGATACT GTACACCATGTTGTTGTCCCAGTAAATCCCAAAACTGACAGACTCTGGGAAAGGCTTGGA AAGAGCCACATTAGAACTGATGATGTACATGCAAAAGATAACACAAGACCTGGTGCTAAT AGTCCAGAGATGTGGTCTGAAGCTATTAAAATCCTGAAAGGGGAGTATGCTGTCCGGGCA ATCAAGGAACATAAGATGGATCAAGCAATTATCTTCTGTAGAACCAAAATTGACTGTGAT AACTTGGAGCAGTACTTTATACAACAAGGAGGAGGACCTGATAAAAAAGGACACCAGTTC TCATGTGTTTGTCTTCATGGTGACAGAAAGCCTCATGAGAGAAAGCAAAACTTGGAAAGA TTTAAGAAAGGAGATGTAAGATTCTTGATTTGCACAGATGTAGCTGCTAGAGGAATTGAT ATCCACGGTGTTCCTTATGTTATAAATGTCACTCTGCCCGATGAAAAGCAAAACTACGTA CATCGAATTGGCAGAGTAGGAAGAGCTGAAAGGATGGGTCTGGCAATTTCCCTGGTGGCA ACAGAAAAAGAAAAGGTTTGGTACCATGTATGTAGCAGCCGTGGAAAAGGGTGTTATAAC ACAAGACTCAAGGAAGATGGAGGCTGTACCATATGGTACAACGAGATGCAGTTACTATCT GAGATAGAAGAACACCTGAACTGTACCATTTCTCAGGTTGAGCCGGATATAAAGGTACCA GTGGATGAATTTGATGGGAAAGTTACCTACGGTCAGAAAAGGGCTGCTGGTGGTGGAAGC TATAAAGGCCATGTGGATATTTTGGCACCTACTGTTCAAGAGTTGGCTGCCCTTGAAAAG GAGGCGCAGACATCTTTCCTGCATCTTGGCTACCTTCCTAACCAGCTGTTCAGAACCTTC TGA Clone variation with respect to NM_004939.1 912 c=>t >OriGene 5' read for NM_004939 unedited
ATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCGCGCCGCCACTGCCGCGCCG TGTCAGTCGGGAGGGAGGGAGCGAGCAGGCGAAGCCGCGGAGGACGGGGTGAAGATGGCG GCCTTCTCCGAGATGGGTGTAATGCCTGAGATTGCACAAGCTGTGGAAGAGATGGATTGG CTCCTCCCAACTGATATCCAGGCTGAATCTATCCCATTGATCTTAGGAGGAGGTGATGTA CTTATGGCTGCAGAAACAGGAAGTGGCAAAACTGGTGCTTTTAGTATTCCAGTTATCCAG ATAGTTTATGAAACTCTGAAAGACCAACAGGAAGGCAAAAAAGGAAAAACAACAATTAAA ACTGGTGCTTCAGTGCTGAACAAATGGCAGATGAACCCATATGACAGAGGATCTGCTTTT GCAATTGGGTCAGATGGTCTTTGTTGTCAAAGCAGAGAAGTAAAGGAATGGCATGGGTGT AGAGCTACTAAAGGATTAATGAAAGGGAAACACTACTATGAAGTATCCTGTCATGACCAA GGGTTATGCAGGGTCGGGTGGTCTACCATGCANGCCTCTNTGGACCTANGTACTGACNNA GTTGGATTTGGCTTTGGTGGAACANGGAAAGAATCCNATNNACAACANNTTTGATATTAT GGAGAGGAATTCACTATGCATGATACCATTGGNATGTACCTGGATATAGATAAGGGACAT GTCCAGTTCTCCAAAAATGGAAAAGATCTTGGTCTGGCATTTGANATAACCACCACATAT GAAAAACCCAGCCCCTCTTTTCTGCCTGTGTTTTGAAGAATGCTGAACTGAATTTTAACT NCGGTGAANAAGGAATTTAAGTTTNCCACAAAAGATGGCTTNGNTGCTCTTTTCCAGCAC CGTATGTTTACATGTCAATCCCACACTCAGGTATGCACAGTGACCCAACAAGTTTCTCCC ATGCCTCGAGCTCTATGNTGGACTTCCGGNAGTACTGACAACTTGATACTCAGCAGTAGA ATC >OriGene 3' read for NM_004939 unedited
TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATAGTCATCAA ATTTATTTTCATTGGGATGCCATTTTTTGAAGAATTCCTAAGACTAATGTTTCTTGACAT GCAAGAGTTAGCATTAATAGCTTACGTTACTATAAATACTGCTGCTTGGAAGCAGTACAA CTGTTTTAGAGTTTTAAGACTACAGACTTTCATTACTCAAATCTTATTCAGCAAATGTAA AAATCAGAAGGTTCTGAACAGCTGGTTAGGAAGGTAGCCAAGATGCAGGAAAGATGTCTG CGCCTCCTTTTCAAGGGCAGCCAACTCTTGAACAGTAGGTGCCAAAATATCCACATGGCC TTTATAGCTTCCACCACCAGCAGCCCTTTTCTGACCGTAGGTAACTTTCCCATCAAATTC ATCCACTGGTACCTTTATATCCGGCTCAACCTGAGAAATGGTACAGTTCAGGTGTTCTTC TATCTCAGATAGTAACTGCATCTCGTTGTACCATATGGTACAGCCTCCATCTTCCTTGAG TCTTGTGTTATAACACCCTTTTCCACGGCTGCTACATACATGGTACCAAACCTTTTCTTT TTCTGTTGCCACCAGGGAAATTGCCAGACCCATCCTTTCAGCTCTTCCTACTCTGCCAAT TCGATGTACGTAGTTNTGCTTTTCATCGCCTAGAGTGACATTTATAACATAAGGGACACC CGGGGATATCAATTCCTCTAGCAGCTACATCTGTGCAAATCAAGAAATCTACATCTCCTA CTTTTAATCTTCCCAAGTTTTGCTTTCTCTCATGAGGCTTTCTGTACCATGAAGACAACA CTGAGAACTGTGTCTTTTTATCAGGCCTCTCCTTGTGATCAAGACTGCTCCAGTTATACA TCATTTTGTCTACAGAGATAATGTTGACCATTTAGTCCTTGATGCCGGAGCTACTCCCTT TAGATTTAATGCTTAACCC |
Restriction Sites | NotI-NotI |
ACCN | NM_004939 |
Insert Size | 2500 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004939.1, NP_004930.1 |
RefSeq Size | 2706 bp |
RefSeq ORF | 2223 bp |
Locus ID | 1653 |
UniProt ID | Q92499 |
Domains | DEAD, helicase_C, SPRY |
Protein Families | Druggable Genome |
Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008] |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208769 | DDX1 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1) |
CNY 5,424.00 |
|
RC208769L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1), Myc-DDK-tagged |
CNY 7,824.00 |
|
RC208769L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1), mGFP tagged |
CNY 5,890.00 |
|
RC208769L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC208769L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1), mGFP tagged |
CNY 5,890.00 |
|
RG208769 | DDX1 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1) |
CNY 7,024.00 |