United States
Germany
Japan
United Kingdom
China
MMP9 (NM_004994) Human Untagged Clone
CAT＃: SC116989
MMP9 (untagged)-Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9)
CNY 7,776.00
| Cited in 8 publications. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLG4B; GELB; MANDP2; MMP-9 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004994 edited
GAATTCGGCACGAGGATGAGCCTCTGGCAGCCCCTGGTCCTGGTGCTCCTGGTGCTGGGC TGCTGCTTTGCTGCCCCCAGACAGCGCCAGTCCACCCTTGTGCTCTTCCCTGGAGACCTG AGAACCAATCTCACCGACAGGCAGCTGGCAGAGGAATACCTGTACCGCTATGGTTACACT CGGGTGGCAGAGATGCGTGGAGAGTCGAAATCTCTGGGGCCTGCGCTGCTGCTTCTCCAG AAGCAACTGTCCCTGCCCGAGACCGGTGAGCTGGATAGCGCCACGCTGAAGGCCATGCGA ACCCCACGGTGCGGGGTCCCAGACCTGGGCAGATTCCAAACCTTTGAGGGCGACCTCAAG TGGCACCACCACAACATCACCTATTGGATCCAAAACTACTCGGAAGACTTGCCGCGGGCG GTGATTGACGACGCCTTTGCCCGCGCCTTCGCACTGTGGAGCGCGGTGACGCCGCTCACC TTCACTCGCGTGTACAGCCGGGACGCAGACATCGTCATCCAGTTTGGTGTCGCGGAGCAC GGAGACGGGTATCCCTTCGACGGGAAGGACGGGCTCCTGGCACACGCCTTTCCTCCTGGC CCCGGCATTCAGGGAGACGCCCATTTCGACGATGACGAGTTGTGGTCCCTGGGCAAGGGC GTCGTGGTTCCAACTCGGTTTGGAAACGCAGATGGCGCGGCCTGCCACTTCCCCTTCATC TTCGAGGGCCGCTCCTACTCTGCCTGCACCACCGACGGTCGCTCCGACGGCTTGCCCTGG TGCAGTACCACGGCCAACTACGACACCGACGACCGGTTTGGCTTCTGCCCCAGCGAGAGA CTCTACACCCGGGACGGCAATGCTGATGGGAAACCCTGCCAGTTTCCATTCATCTTCCAA GGCCAATCCTACTCCGCCTGCACCACGGACGGTCGCTCCGACGGCTACCGCTGGTGCGCC ACCACCGCCAACTACGACCGGGACAAGCTCTTCGGCTTCTGCCCGACCCGAGCTGACTCG ACGGTGATGGGGGGCAACTCGGCGGGGGAGCTGTGCGTCTTCCCCTTCACTTTCCTGGGT AAGGAGTACTCGACCTGTACCAGCGAGGGCCGCGGAGATGGGCGCCTCTGGTGCGCTACC ACCTCGAACTTTGACAGCGACAAGAAGTGGGGCTTCTGCCCGGACCAAGGATACAGTTTG TTCCTCGTGGCGGCGCATGAGTTCGGCCACGCGCTGGGCTTAGATCATTCCTCAGTGCCG GAGGCGCTCATGTACCCTATGTACCGCTTCACTGAGGGGCCCCCCTTGCATAAGGACGAC GTGAATGGCATCCGGCACCTCTATGGTCCTCGCCCTGAACCTGAGCCACGGCCTCCAACC ACCACCACACCGCAGCCCACGGCTCCCCCGACGGTCTGCCCCACCGGACCCCCCACTGTC CACCCCTCAGAGCGCCCCACAGCTGGCCCCACAGGTCCCCCCTCAGCTGGCCCCACAGGT CCCCCCACTGCTGGCCCTTCTACGGCCACTACTGTGCCTTTGAGTCCGGTGGACGATGCC TGCAACGTGAACATCTTCGACGCCATCGCGGAGATTGGGAACCAGCTGTATTTGTTCAAG GATGGGAAGTACTGGCGATTCTCTGAGGGCAGGGGGAGCCGGCCGCAGGGCCCCTTCCTT ATCGCCGACAAGTGGCCCGCGCTGCCCCGCAAGCTGGACTCGGTCTTTGAGGAGCCGCTC TCCAAGAAGCTTTTCTTCTTCTCTGGGCGCCAGGTGTGGGTGTACACAGGCGCGTCGGTG CTGGGCCCGAGGCGTCTGGACAAGCTGGGCCTGGGAGCCGACGTGGCCCAGGTGACCGGG GCCCTCCGGAGTGGCAGGGGGAAGATGCTGCTGTTCAGCGGGCGGCGCCTCTGGAGGTTC GACGTGAAGGCGCAGATGGTGGATCCCCGGAGCGCCAGCGAGGTGGACCGGATGTTCCCC GGGGTGCCTTTGGACACGCACGACGTCTTCCAGTACCGAGAGAAAGCCTATTTCTGCCAG GACCGCTTCTACTGGCGCGTGAGTTCCCGGAGTGAGTTGAACCAGGTGGACCAAGTGGGC TACGTGACCTATGACATCCTGCAGTGCCCTGAGGACTAGGGCTCCCGTCCTGCTTTGGCA GTGCCATGTAAATCCCCACTGGGACCAACCCTGGGGAAGGAGCCAGTTTGCCGGATACAA ACTGGTATTCTGTTCTGGAGGAAAGGGAGGAGTGGAGGTGGGCTGGGCCCTCTCTTCTCA CCTTTGTTTTTTGTTGGAGTGTTTCTAATAAACTTGGATTCTCTAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_004994 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGAGCCTCTGGCAGCC CCTGGTCCTGGTGCTCCTGGTGCTGGGCTGCTGCTTTGCTGCCCCCAGACAGCGCCAGTC CACCCTTGTGCTCTTCCCTGGAGACCTGAGAACCAATCTCACCGACAGGCAGCTGGCAGA GGAATACCTGTACCGCTATGGTTACACTCGGGTGGCAGAGATGCGTGGAGAGTCGAAATC TCTGGGGCCTGCGCTGCTGCTTCTCCAGAAGCAACTGTCCCTGCCCGAGACCGGTGAGCT GGATAGCGCCACGCTGAAGGCCATGCGAACCCCACGGTGCGGGGTCCCAGACCTGGGCAG ATTCCAAACCTTTGAGGGCGACCTCAAGTGGCACCACCACAACATCACCTATTGGATCCA AAACTACTCGGAAGACTTGCCGCGGGCGGTGATTGACGACGCCTTTGCCCGCGCCTTCGC ACTGTGGAGCGCGGTGACGCCGCTCACCTTCACTCGCGTGTACAGCCGGGACGCAGACAT CGTCATCCAGTTTGGTGTCGCGGAGCACGGAGACGGGTATCCCTTCGACGGGAAGGACGG GCTCCTGGCACACGCCTTTCCTCCTGGCCCCGGCATTCAGGGAGACGCCCATTTCGACGA TGACGAGTTGTGGTCCCTGGGCAAGGGCGTCGTGGTTCCAACTCGGTTTGGAAACGCAGA TGGCGCGGNCTGCCACTTCCCCTTCATCTTCGNAGGCCGCTCCTACTCTGCCTGCACCAC CGACGGTCGCTCCGACGGCTTGCCCTGGTGCAGTACCACGGCCAACTACGACACCGACGA CCGGNTTGGCTTNCTGCCCAGCGAGAGACTCTACACCCGGGACNGGCATGCTGATGGGAA ACCCTGNCAGTTNCATTCATCTTCAGGNCCATACTACTNCGCTGCCCN >OriGene 3' read for NM_004994 unedited
TTTTTTTTTTTNNTNTNNTNNTTTTTTTNTTTTTTGTNNTTTTTTTTTTTTCGATTTCTT CAGCAACACTTCCACAAAAACAAGGTGTACAACACGGGCCCAGTCCACTCCCTCCTCCTT TCCTCCAGAACAGATACCAGTTTGTATCCGGCAAACTGGCTCCTTCCCCAGGTTGGTCCC AGCGGGGATCCACATGGCACTGCCAACCCACGACGGCCCCCCCTAGTCCTCAGGGCACTG CCGGCACGTCACAGGTCCACGTCCCCCACTTGGCCCACCTGGTACAACTCACTTCGGGAA CTCACGCTTCATTCTTAATCCGTTCTCGCCCCAAATAGGCTCTCTCCTTCCTCCTGCTAC CCGTCCCCGCGTGCCCACACGCTCCCCGGTTGACCTTCCTGTCCCCCCTCCTGGCCCTCC CGGTGATTTACCCTCCTCTGCCTCCACTCCCTCACCTCCATAGGTCCTCGTTCTCTCACC ATCTCCTCTCTCCCCCTGCCCCCTCCCCAGGGTCACCTCCCCCCTGCGCCCACGTCCGCC TCCCCATGCCTCTTTTTGTTCACACCCCTCGCTTTCGATTTATTGCCGCACCCTGAGTAC ACCCCTCACCCCAGCTGCCCATCTTCACCCATCTAATCCTTCCCTGTCACGCCGTCCCCC CCCCCCACCGCACCTCCCCGCCTGCCACCGCCCCTGCCGCCCCCACCCCTCCCCCCCAAC CCCAAAAGCGCCCCCTGCCATCTTGGCCTCCCTCCTTCTCTTTATCAAACAACACAAACT CTGCCCTTCCCTCGTAAAATTTACTATTGCCACCCCCACCCCCCCCGTATGACACGCATA CCATGTTCATATTCCGCCCATATTTCACCCTATGAACTTACATTGTCCACTTCACGGGCC CGCATTATGGGCTCACCCTCTTGCGCCCCCCCCCTGTCTTCACTCCTCCCCGCCCTCCCC CACCTCTTCCCTCCCTCCCCCGCCCCCCTCCACCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004994 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004994.2, NP_004985.2 |
| RefSeq Size | 2387 bp |
| RefSeq ORF | 2124 bp |
| Locus ID | 4318 |
| UniProt ID | P14780 |
| Domains | FN2, hemopexin, Peptidase_M10, ZnMc, PT |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Bladder cancer, Leukocyte transendothelial migration, Pathways in cancer |
| Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] |
Citations (8)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
GBM-targeted oHSV armed with matrix metalloproteinase 9 enhances anti-tumor activity and animal survival
,Sette, P;Amankulor, N;Li, A;Marzulli, M;Leronni, D;Zhang, M;Goins, W;Kaur, B;Bolyard, C;Cripe, T;Yu, J;Chiocca, E;Glorioso, J;Grandi, P;,
Molecular Therapy - Oncolytics
[MMP9]
|
|
EGF stimulates glioblastoma metastasis by induction of matrix metalloproteinase-9 in an EGFR-dependent mechanism
,Chen, XC;Wei, XT;Guan, JH;Shu, H;Chen, D;,
Oncotarget
,PubMed ID 29029486
[MMP9]
|
|
Diverse matrix metalloproteinase functions regulate cancer amoeboid migration
,Orgaz, JL;Pandya, P;Dalmeida, R;Karagiannis, P;Sanchez-Laorden, B;Viros, A;Albrengues, J;Nestle, FO;Ridley, AJ;Gaggioli, C;Marais, R;Karagiannis, SN;Sanz-Moreno, V;,
Nat Commun
,PubMed ID 24963846
[MMP9]
|
|
Vaccinia virus-mediated intra-tumoral expression of matrix metalloproteinase 9 enhances oncolysis of PC-3 xenograft tumors
,null,
BMC Cancer
,PubMed ID 22917220
[MMP9]
|
|
The Transcription Factor SPDEF Suppresses Prostate Tumor Metastasis
,Joshua J. Steffan, Sweaty Koul, Randall B. Meacham, and Hari K. Koul,
J. Biol. Chem., Aug 2012; 287: 29968 - 29978.
[MMP9]
|
|
siRNA-Mediated Downregulation of MMP-9 and uPAR in Combination with Radiation Induces G2/M Cell-Cycle Arrest in Medulloblastoma
,Purna Chandra Nagaraju Ganji, Arun Kumar Nalla, Reshu Gupta, Sanjeeva Mohanam, Meena Gujrati, Dzung H. Dinh, and Jasti S. Rao,
Mol. Cancer Res., Jan 2011; 9: 51 - 66
[MMP9]
|
|
Ectopic matrix metalloproteinase-9 expression in human brain tumor cells enhances oncolytic HSV vector infection
,C-S Hong, W Fellows, A Niranjan, S Alber, S Watkins, J B Cohen, J C Glorioso, P Grandi,
Gene Therapy (13 May 2010) doi:10.1038/gt.2010.66
[MMP9]
|
|
The Hemopexin Domain of Matrix Metalloproteinase-9 Activates Cell Signaling and Promotes Migration of Schwann Cells by Binding to Low-Density Lipoprotein Receptor-Related Protein
,Elisabetta Mantuano, Gen Inoue, Xiaoqing Li, Kazuhisa Takahashi, Alban Gaultier, Steven L. Gonias, and W. Marie Campana,
J. Neurosci., Nov 2008; 28: 11571 - 11582.
[MMP9]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202872 | MMP9 (Myc-DDK-tagged)-Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9) |
CNY 7,768.00 |
|
| RC202872L1 | Lenti ORF clone of Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9), Myc-DDK-tagged |
CNY 10,168.00 |
|
| RC202872L2 | Lenti ORF clone of Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9), mGFP tagged |
CNY 7,700.00 |
|
| RC202872L3 | Lenti ORF clone of Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9), Myc-DDK-tagged |
CNY 10,168.00 |
|
| RC202872L4 | Lenti ORF clone of Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9), mGFP tagged |
CNY 10,168.00 |
|
| RG202872 | MMP9 (tGFP-tagged) - Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9) |
CNY 9,368.00 |
