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Calpain 1 (CAPN1) (NM_005186) Human Untagged Clone
CAT＃: SC116897
CAPN1 (untagged)-Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2
CNY 5,240.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CANP; CANP1; CANPL1; muCANP; muCL; SPG76 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_005186 edited
GCGGCCGCGAATTCGGCACGAGGGGGAAGCCAGCCTCAGGGACTGCAGCGACCCCCAAAC ACCCCTCCCCCAGATGTCGGAGGAGATCATCACGCCGGTGTACTGCACTGGGGTGTCAGC CCAAGTGCAGAAGCAGCGGGCCAGGGAGCTGGGCCTGGGCCGCCATGAGAATGCCATCAA GTACCTGGGCCAGGATTATGAGCAGCTGCGGGTGCGATGCCTGCAGAGTGGGACCCTCTT CCGTGATGAGGCCTTCCCCCCGGTACCCCAGAGCCTGGGTTACAAGGACCTGGGTCCCAA TTCCTCCAAGACCTATGGCATCAAGTGGAAGCGTCCCACGGAACTGCTGTCAAACCCCCA GTTCATTGTGGATGGAGCTACCCGCACAGACATCTGCCAGGGAGCACTGGGGGACTGCTG GCTCTTGGCGGCCATCGCCTCCCTCACTCTCAACGACACCCTCCTGCACCGAGTGGTTCC GCACGGCCAGAGCTTCCAGAATGGCTATGCCGGCATCTTCCATTTCCAGCTGTGGCAATT TGGGGAGTGGGTGGACGTGGTCGTGGATGACCTGCTGCCCATCAAGGACGGGAAGCTAGT GTTCGTGCACTCTGCCGAAGGCAACGAGTTCTGGAGCGCCCTGCTTGAGAAGGCCTATGC CAAGGTAAATGGCAGCTACGAGGCCCTGTCAGGGGGCAGCACCTCAGAGGGCTTTGAGGA CTTCACAGGCGGGGTTACCGAGTGGTACGAGTTGCGCAAGGCTCCCAGTGACCTCTACCA GATCATCCTCAAGGCGCTGGAGCGGGGCTCCCTGCTGGGCTGCTCCATAGACATCTCCAG CGTTCTAGACATGGAGGCCATCACTTTCAAGAAGTTGGTGAAGGGCCATGCCTACTCTGT GACCGGGGCCAAGCAGGTGAACTACCGAGGCCAGGTGGTGAGCCTGATCCGGATGCGGAA CCCCTGGGGCGAGGTGGAGTGGACGGGAGCCTGGAGCGACAGCTCCTCAGAGTGGAACAA CGTGGACCCATATGAACGGGACCAGCTCCGGGTCAAGATGGAGGACGGGGAGTTCTGGAT GTCATTCCGAGACTTCATGCGGGAGTTCACCCGCCTGGAGATCTGCAACCTCACACCCGA CGCCCTCAAGAGCCGGACCATCCGCAAATGGAACACCACACTCTACGAAGGCACCTGGCG GCGGGGGAGCACCGCGGGGGGCTGCCGAAACTACCCAGCCACCTTCTGGGTGAACCCTCA GTTCAAGATCCGGCTGGATGAGACGGATGACCCGGACGACTACGGGGACCGCGAGTCAGG CTGCAGCTTCGTGCTCGCCCTTATGCAGAAGCACCGTCGCCGCGAGCGCCCCTTCGGCCG CGACATGGAGACTATTGGCTTCGCGGTCTACGAGGTCCCTCCGGAGCTGGTGGGCCAGCC GGCCGTACACTTGAAGCGTGACTTCTTCCTGGCCAATGCGTCTCGGGCGCGCTCAGAGCA GTTCATCAACCTGCGAGAGGTCAGCACCCGCTTCCGCCTGCCACCCGGGGAGTATGTGGT GGTGCCCTCCACCTTCGAGCCCAACAAGGAGGGCGACTTCGTGCTGCGCTTCTTCTCAGA GAAGAGTGCTGGGACTGTGGAGCTGGATGACCAGATCCAGGCCAATCTCCCCGATGAGCA AGTGCTCTCAGAAGAGGAGATTGACGAGAACTTCAAGGCCCTCTTCAGGCAGCTGGCAGG GGAGGACATGGAGATCAGCGTGAAGGAGTTGCGGACAATCCTCAATAGGATCATCAGCAA ACACAAAGACCTGCGGACCAAGGGCTTCAGCCTAGAGTCGTGCCGCAGCATGGTGAACCT CATGGATCGTGATGGCAATGGGAAGCTGGGCCTGGTGGAGTTCAACATCCTGTGGAACCG CATCCGGAATTACCTGTCCATCTTCCGGAAGTTTGACCTGGACAAGTCGGGCAGCATGAG TGCCTACGAGATGCGGATGGCCATTGAGTCGGCAGGCTTCAAGCTCAACAAGAAGCTGTA CGAGCTCATCATCACCCGCTACTCGGAGCCCGACCTGGCGGTCGACTTTGACAATTTCGT TTGCTGCCTGGTGCGGCTAGAGACCATGTTCCGATTTTTCAAAACTCTGGACACAGATCT GGATGGAGTTGTGACCTTTGACTTGTTTAAGTGGTTGCAGCTGACCATGTTTGCATGAGG CAGGGACTCGGTCCCCCTTGCCGTGCTCCCCTCCCTCCTCGTCTGCCAAGCCTCGCCTCC TACCACACCACACCAGGCCACCCCAGCTGCAAGTGCCTTCCTTGGAGCAGAGAGGCAGCC TCGTCCTCCTGTCCCCTCTCCTCCCAGCCACCATCGTTCATCTGCTCCGGGCAGAACTGT GTGGCCCCTGCCTGTGCCAGCCATGGGCTCGGGATGGACTCCCTGGGCCCCACCCATTGC CAAGCCAGGAAGGCAGCTTTCGCTTGTTCCTGCCTCGGGACAGCCCCGGGTTTCCCCAGC ATCCTGATGTGTCCCCTCTCCCCACTTCAGAGGCCACCCACTCAGCACCACCGGCCTGGC CTTGCCTGCAGACTATAAACTATAACCACTAGCTCGACACAGTCTGCAGTCCAGGCGTGT GGAGCCGCCTCCCGGCTCGGGGAGGCCCCGGGGCTGGGAACGCCTGTGCCTTCCTGCGCC GAAGCCAACGCCCCCTCTGTCCTTCCCTGGCCCTGCTGCCGACCAGGAGCTGCCCAGCCT GTGGGCGGTCGGCCTTCCCTCCTTCGCTCCTTTTTTATATTAGTGATTTTAAAGGGGACT CTTCAGGGACTTGTGTACTGGTTATGGGGGTGCCAGAGGCACTAGGCTTGGGGTGGGGAG GTTCCCGTGTTCCATATAGAGGAACCCCAAATAA >OriGene 5' read for NM_005186 unedited
ACTAGNGATTTTGAAATACCCACTTTCTATGAGGGCGGCACAAAACTTCGGCACGAGGGG GAAGCCAGCCTCAGGGACTGCAGCGACCCCCAAACACCCCTCCCCCAGATGTCGGAGGAG ATCATCACGCCGGTGTACTGCACTGGGGTGTCAGCCCAAGTGCAGAAGCAGCGGGCCAGG GAGCTGGGCCTGGGCCGCCATGAGAATGCCATCAAGTACTTGTTCCAGGATTATGAGCAG CTGCGGGTGCGATGCCTGCAGAGTGGGACCCTCTTCCGTGATGAGGCCTTCCCCCCGGTA CCCCAGAGCCTGGGTTACAAGGACCTGGGTCCCAATTCCTCCAAGACCTATGGCATCAAG TGGAAGCGTCCCACGGAACTGCTGTCAAACCCCCAGTTCATTGTGGATGGAGCTACCCGC ACAGACATCTGCCAGGGAGCACTGGGGGACTGCTGGCTCTTGGCGGCCATCGCCTCCCTC ACTCTCAACGACACCCTCCTGCACCGAGTGGTTCCGCACGGCCAGAGCTTCCAGAATGGC TATGCCGGCATCTTCCATTTCCAGCTGTGGCAATTTGGGGAGTGGGTGGACGTGGTCGTG GATGACCTGCTGCCCATCAAGGACGGGAAGCTAGTGTTCGTGCACTCTGCCGAAGCAACG AGTTCTGGAGCGCCCTGCTTGAGAAGGCCTATGCCAAGGTAAATGGCAGCTACGAAGCCC TGTCAGGGGGCAGCACCTCAGAGGGCTTTGAGGACTTCACAGGCGGNNGTACCGAGTGGT ACGAGTTGCGCAAGGCTNCCAGTGACCTCCTACAGATCATCCTCAAGGCGCTTGGAGCGG GGCTCCCTGCTGGGCTGGCTCATAGACATCTCCAGCGTTCTAAGAATGGAGGCCATCACT TTCAAAGAGTTGGTTGAAGGCCAATGCTTACTCTGTGACCGGGGCAAACAG >OriGene 3' read for NM_005186 unedited
CCCCCCAATACTGTGCACCGCGGCCGCATCTNACGAGCGGTTTTTTTTTTTTTTTTTTCA CAGCACGAAGTGGGGCCCTTTTATTATTTGGGGTTCCTCTATATGGAACACGGGAACCTC CCCACCCCAAGCCTAGTGCCTCTGGCACCCCCATAACCAGTACACAAGTCCCTGAAGAGT CCCCTTTAAAATCACTAATATAAAAAAGGAGCGAAGGAGGGAAGGCCGACCGCCCACAGG CTGGGCAGCTCCTGGTCGGCAGCAGGGCCAGGGAAGGACAGAGGGGGCGTTGGCTTCGGC GCAGGAAGGCACAGGCGTTCCCAGCCCCGGGGCCTCCCCGAGCCGGGAGGCGGCTCCACA CGCCTGGACTGCAAACTGTGTCGAGCTAGTGGTTATAGTTTATAGTCTGCAGGCAAGGCC AGGCCGGTGGTGCTGAGTGGGTGGCCTCTGAAGTGGGGAGAGGGGACACATCAGGATGCT GGGGAAACCCGGGGCTGTCCCGAGGCAGGAACAAGCGAAAGCTGCCTTCCTGGCTTGGCA ATGGGTGGGGCCCAGGGAGTCCATCCCGAGCCCATGGCTGGCACAGGCAGGGGCCACACA GTTCTGCCCGGAGCAGATGAACGATGGTGGCTGGGAGGAGAGGGGACAGGAGGACAAGGC TGCCTCTCTGCTCCAAGGAAGGCACTTGCAGCTGGGGTGGCCTGCTGTGGTGTGGTAGGA GGCCAGGCTTGGCACACTAGGAGGGAGGGGAGCACGGCAGGGGGACCGAGTCCTGCCTCA TGCAAACATGTTCAGCTGCAACCCTTAAACAAGTCAAAGTCACAACTCCATCCAGATCTG TGTCCACAGTTTTTGAAAATCGAACATGGTCTTTACCGCACCCAGCAGCAACGAAATGTC AAGTCGACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005186 |
| Insert Size | 2880 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005186.2, NP_005177.2 |
| RefSeq Size | 3007 bp |
| RefSeq ORF | 2145 bp |
| Locus ID | 823 |
| UniProt ID | P07384 |
| Domains | Calpain_III, EFh |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Alzheimer's disease, Apoptosis |
| Gene Summary | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Glycosylation stabilizes hERG channels on the plasma membrane by decreasing proteolytic susceptibility
,Lamothe, SM;Hulbert, M;Guo, J;Li, W;Yang, T;Zhang, S;,
FASEB J.2017
,PubMed ID 29162701
[CAPN1]
|
|
The Human Ether-a-go-go-Related Gene (hERG) Potassium Channel Represents an Unusual Target for Protease-mediated Damage
,Lamothe, SM;Guo, J;Li, W;Yang, T;Zhang, S;,
J. Biol. Chem.
,PubMed ID 27502273
[CAPN1]
|
|
Calpain Determines the Propensity of Adult Hippocampal Neural Stem Cells to Autophagic Cell Death Following Insulin Withdrawal
,Chung, KM;Park, H;Jung, S;Ha, S;Yoo, SJ;Woo, H;Lee, HJ;Kim, SW;Kim, EK;Moon, C;Yu, SW;,
Stem Cells
,PubMed ID 26086870
[CAPN1]
|
|
Caspase-dependent cell death-associated release of nucleosome and damage-associated molecular patterns
,Yoon, S;Park, SJ;Han, JH;Kang, JH;Kim, JH;Lee, J;Park, S;Shin, HJ;Kim, K;Yun, M;Chwae, YJ;,
Cell Death Dis
,PubMed ID 25356863
[CAPN1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200964 | CAPN1 (Myc-DDK-tagged)-Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2 |
CNY 5,232.00 |
|
| RC200964L1 | Lenti ORF clone of Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2, Myc-DDK-tagged |
CNY 7,632.00 |
|
| RC200964L2 | Lenti ORF clone of Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC200964L3 | Lenti ORF clone of Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200964L4 | Lenti ORF clone of Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2, mGFP tagged |
CNY 7,632.00 |
|
| RG200964 | CAPN1 (tGFP-tagged) - Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2 |
CNY 6,832.00 |
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