EDG3 (S1PR3) (NM_005226) Human Untagged Clone

CAT#: SC116857

S1PR3 (untagged)-Human sphingosine-1-phosphate receptor 3 (S1PR3)



  "NM_005226" in other vectors (6)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Anti-S1PR3 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EDG-3; EDG3; LPB3; S1P3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116857 sequence for NM_005226 edited (data generated by NextGen Sequencing)
ATGGCAACTGCCCTCCCGCCGCGTCTCCAGCCGGTGCGGGGGAACGAGACCCTGCGGGAG
CATTACCAGTACGTGGGGAAGTTGGCGGGCAGGCTGAAGGAGGCCTCCGAGGGCAGCACG
CTCACCACCGTGCTCTTCTTGGTCATCTGCAGCTTCATCGTCTTGGAGAACCTGATGGTT
TTGATTGCCATCTGGAAAAACAATAAATTTCACAACCGCATGTACTTTTTCATTGGCAAC
CTGGCTCTCTGCGACCTGCTGGCCGGCATCGCTTACAAGGTCAACATTCTGATGTCTGGC
AAGAAGACGTTCAGCCTGTCTCCCACGGTCTGGTTCCTCAGGGAGGGCAGTATGTTCGTG
GCCCTTGGGGCGTCCACCTGCAGCTTACTGGCCATCGCCATCGAGCGGCACTTGACAATG
ATCAAAATGAGGCCTTACGACGCCAACAAGAGGCACCGCGTCTTCCTCCTGATCGGGATG
TGCTGGCTCATTGCCTTCACGCTGGGCGCCCTGCCCATTCTGGGCTGGAACTGCCTGCAC
AATCTCCCTGACTGCTCTACCATCCTGCCCCTCTACTCCAAGAAGTACATTGCCTTCTGC
ATCAGCATCTTCACGGCCATCCTGGTGACCATCGTGATCCTCTACGCACGCATCTACTTC
CTGGTGAAGTCCAGCAGCCGTAAGGTGGCCAACCACAACAACTCGGAGCGGTCCATGGCA
CTGCTGCGGACCGTGGTGATTGTGGTGAGCGTGTTCATCGCCTGCTGGTCCCCACTCTTC
ATCCTCTTCCTCATTGATGTGGCCTGCAGGGTGCAGGCGTGCCCCATCCTCTTCAAGGCT
CAGTGGTTCATCGTGTTGGCTGTGCTCAACTCCGCCATGAACCCGGTCATCTACACGCTG
GCCAGCAAGGAGATGCGGCGGGCCTTCTTCCGTCTGGTCTGCAACTGCCTGGTCAGGGGA
CGGGGGGCCCGCGCCTCACCCATCCAGCCTGCGCTCGACCCAAGCAGAAGTAAATCAAGC
AGCAGCAACAATAGCAGCCACTCTCCGAAGGTCAAGGAAGACCTGCCCCACACAGCCCCC
TCATCCTGCATCATGGACAAGAACGCAGCACTTCAGAATGGGATCTTCTGCAACTGA

Clone variation with respect to NM_005226.2
>OriGene 5' read for NM_005226 unedited
ACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGTTCCGGGGACGGGCAACAGGGACTCA
GGGACCAGAAGGCGGCTGCAGGACGCGACCGAGCAGGACCCCAGGCCCGGGAACGACGTC
GCGAGCGCTGAGACTGCCGGGCCTCCCAGCCCATCTGGCATTCGAGCGCAGGACCGGGCG
CCCCGGCACCGCCGCGCGCCACCCGCTAGGATGCCGGTGGCCCCAGCGCCCTCAGCCGAC
GGAGGAGCCCTTTTTCAACGCCCTCGCTGGAGTCTGGCCTGCACGCCTTGCTGAATGAAG
CCGGAACCTCAGCCCCGCTTCCCTTTGAAATGAATGTTCCTGGGGCGCCCTCTCGTGGAT
TTTGGAGCTAATCGTCTGTGAATGCCAAGTGATGGCAACTGCCCTCCCGCCGCGTCTCCA
GCCGGTGCGGNGGAACGAGACCCTGCGGGAGCATTACCAGTACGTGGGGAAGTTGGCGGG
CAGGCTGAAGGAGGCCTCCGAGGGCAGCACGCTCACCACCGTGCTCTTCTGGGTCATCTG
CAGCTTCATCGTCTGGGAGAACCTGATGGTTNTGATTGCCATCTGGNAAAAACATANATN
TCACAACCGCATGTACTTTTTCATTGGCAACCTGGCTCTCTGCGACCTGCTGGGCCGCAT
CGCTTACAAGGTCACATTCTGATGTCTGGCAAGAAGACGTTCAGCCTGTCTCCCACGGTC
TGGGTCCTCAGGGAGGGCAGTATGTTCGTTGCCCCTTGGGGCGTCCACCTGCAGCTTACT
GGCCATCGCCATCGAGCGGCACTTGGACATGATCAAAATGAGGCCTTACGACGCCACAGG
AGGCACNGNGTTTTCTCCTGACGGGATGTGCTGCTCATTGCCTCACCCGGGGCGCCTGCC
ATTTTGGCTGAACTGCTGCCAATTCCCTGATGTTTACATCCTGCCCTTACTCAGAAGACA
TGGCTCTGATACATTTTACGCCTCTGTGACAATGGATCTTACCCCCTTC
>OriGene 3' read for NM_005226 unedited
CGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGGTTTCCACAAAGGCTT
TTGGTTTTATTATTATTATTTTTAACAAACATACACTTGGAAATAGTAAAGGTTTCTAAT
TGAAAAGAGACCTAATGCATAGTTTCTCTGCAGCATAGAAGACCACCTGTTTCCAAACTT
CAAAGTTAGGGCTTTGAAACATTCCCTGGTAAATTATCTCCAGCTGGCTAGCTAGTTAGG
CATATTGGTGCACATTGGTATCAAAGGTGATTATTTTTTGCATTTAGCAAATGAGCGCTA
TTACTTTTTTGATGTATTTTTAATTCAGATTGCTCAAAAGTCTTGTTATTTCAAGGGAGC
CTTATGTCATACCACAAATATTGATACCTCCTAGTGGAAATGGTGGCTGTGGGCTCCCTA
GTAGAGTTAATGGATGAAGGCTGAGATACTTAGATACTGCGGACATCAGGGTTATGGAGC
AAAGTGTTATGAACAATAGCTACAGAGAGCTCCAAGTCTCCCAAAGCAACTCATAACTAT
AACACAGATAAAGTTCCAAAGTCATTTCATCACCTATGTGAAAATGCAATACTGAATGTT
TTGGCTCTCTGATGCATTTTGGTGGTGGTTTATTTATTATTATTATTATTTTTTGAGACA
GAGGGTCGCTCTGTCACCAGCCTGGGGAATGCANTGGTGCGATCTTGGGCTCACTGCAAT
CTCCGGCTCCCGGGTTTGAGCAACTCTTCAGCCTCAGCCCTCCCGAGTAGCTGGGACTAC
AGGCACGTGCTATCCGATTCAGCTAATTTTTGTATTTTTAGTAGAAACAGGATTTCACTT
TGTTGGGCCAGGCTGGTCCCAACTTCCTGGACTCAGGGTGATCCCCCCGCCTCTAGCCTC
CCAAAGTGCTTGGGATTACAGGGGGGAAGCCCCTGGCCCCAACCCTGTTGTGGTTTTCTT
AATCCCGTCCTTAAACGTTTTACT
Restriction Sites NotI-NotI     
ACCN NM_005226
Insert Size 4350 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005226.2, NP_005217.2
RefSeq Size 4221 bp
RefSeq ORF 1137 bp
Locus ID 1903
UniProt ID Q99500
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Gene Summary This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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