ERp57 (PDIA3) (NM_005313) Human Untagged Clone

CAT#: SC116818

PDIA3 (untagged)-Human protein disulfide isomerase family A, member 3 (PDIA3)



  "NM_005313" in other vectors (6)

CNY 3,768.00


货期*
现货

规格
    • 10 ug

Cited in 2 publications.

Product images

经常一起买 (4)
PDIA3 (ERp57) mouse monoclonal antibody, clone OTI3E1 (formerly 3E1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HEL-S-93n; HEL-S-269; HsT17083; P58; PI-PLC
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116818 sequence for NM_005313 edited (data generated by NextGen Sequencing)
ATGCGCCTCCGCCGCCTAGCGCTGTTCCCGGGTGTGGCGCTGCTTCTTGCCGCGGCCCGCCTCGCCGCTG
CCTCCGACGTGCTAGAACTCACGGACGACAACTTCGAGAGTCGCATCTCCGACACGGGCTCTGCGGGCCT
CATGCTCGTCGAGTTCTTCGCTCCCTGGTGTGGACACTGCAAGAGACTTGCACCTGAGTATGAAGCTGCA
GCTACCAGATTAAAAGGAATAGTCCCATTAGCAAAGGTTGATTGCACTGCCAACACTAACACCTGTAATA
AATATGGAGTCAGTGGATATCCAACCCTGAAGATATTTAGAGATGGTGAAGAAGCAGGTGCTTATGATGG
ACCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAGCAGGCAGGACCAGCTTCAGTGCCTCTCAGG
ACTGAGGAAGAATTTAAGAAATTCATTAGTGATAAAGATGCCTCTATAGTAGGTTTTTTCGATGATTCAT
TCAGTGAGGCTCACTCCGAGTTCCTAAAAGCAGCCAGCAACTTGAGGGATAACTACCGATTTGCACATAC
GAATGTTGAGTCTCTGGTGAACGAGTATGATGATAATGGAGAGGGTATCATCTTATTTCGTCCTTCACAT
CTCACTAACAAGTTTGAGGACAAGACTGTGGCATATACAGAGCAAAAAATGACCAGTGGCAAAATTAAAA
AGTTTATCCAGGAAAACATTTTTGGTATCTGCCCTCACATGACAGAAGACAATAAAGATTTGATACAGGG
CAAGGACTTACTTATTGCTTACTATGATGTGGACTATGAAAAGAACGCTAAAGGTTCCAACTACTGGAGA
AACAGGGTAATGATGGTGGCAAAGAAATTCCTGGATGCTGGGCACAAACTCAACTTTGCTGTAGCTAGCC
GCAAAACCTTTAGCCATGAACTTTCTGATTTTGGCTTGGAGAGCACTGCTGGAGAGATTCCTGTTGTTGC
TATCAGAACTGCTAAAGGAGAGAAGTTTGTCATGCAGGAGGAGTTCTCGCGTGATGGGAAGGCTCTGGAG
AGGTTCCTGCAGGATTACTTTGATGGCAATCTGAAGAGATACCTGAAGTCTGAACCTATCCCAGAGAGCA
ATGATGGGCCTGTGAAGGTAGTGGTAGCAGAGAATTTTGATGAAATAGTGAATAATGAAAATAAAGATGT
GCTGATTGAATTTTATGCCCCTTGGTGTGGTCATTGTAAGAACCTGGAGCCCAAGTATAAAGAACTTGGC
GAGAAGCTCAGCAAAGACCCAAATATCGTCATAGCCAAGATGGATGCCACAGCCAATGATGTGCCTTCTC
CATATGAAGTCAGAGGTTTTCCTACCATATACTTCTCTCCAGCCAACAAGAAGCTAAATCCAAAGAAATA
TGAAGGTGGCCGTGAATTAAGTGATTTTATTAGCTATCTACAAAGAGAAGCTACAAACCCCCCTGTAATT
CAAGAAGAAAAACCCAAGAAGAAGAAGAAGGCACAGGAGGATCTCTAA

Clone variation with respect to NM_005313.4
46 c=>n;47 t=>n;48 t=>n;49 g=>n;50 c=>n;51 c=>n;52 g=>n;53 c=>n;162 c=>t;1224 c=>t
>OriGene 5' read for NM_005313 unedited
CGGTGCAGATTTGTATACGACTCACTATAGGCGGCCGCGCAATCGGCACGAGGGCGGGTT
AGTGGTCGCGCGCCCGACCTCCGCAGTCCCAGCCGAGCCGCGACCCTTCCGGCCGTCCCC
ACCCCACCTCGCCGCCATGCGCCTCCGCCGCCTAGCGCTGTTCCCGGGTGTGGCGCTGCT
TCTTGCCGCGGCCCGCCTCGCCGCTGCCTCCGACGTGCTAGAACTCACGGACGACAACTT
CGAGAGTCGCATCTCCGACACGGGCTCTGCGGGCCTCATGCTCGTCGAGTTCTTCGCTCC
CTGGTGTGGACACTGCAAGAGACTTGCACCTGAGTATGAAGCTGCAGCTACCAGATTAAA
AGGAATAGTCCCATTAGCAAAGGTTGATTGCACTGCCAACACTAACACCTGTAATAAATA
TGGAGTCAGTGGATATCCAACCCTGAAGATATTTAGAGATGGTGAAGAAGCAGGTGCTTA
TGATGGACCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAGCAGGCAGGACCAGC
TTCAGTGCCTCTCAGGACTGAGGAAGAATTTAAGAAATTCATTAGTGATAAAGATGCCTC
TATAGTAGGTTTTTTCGATGATTCATTCAGTGAGGCTCACTCCGAGTTCCTAGAAGCAGC
CAGCAACTTGAGGGATAACTACCGATTTGCACATACCAATGTTGAGTCTCTGGTGAACGA
GTATGATGATCATGGAGCAGGGATCATCTTATTTCGTCCTTCACATCTCACTAACCAAGT
TTGAAGACAAGACTGTGGCATATACCGAGCCACAAATGACCCGTGGCAACATTTAACAGG
TTATCCCGGACAACATTTTTGGTATCTGCCCTCCCTTGACCGAAGACACTTCGATTTGTT
CCG
>OriGene 3' read for NM_005313 unedited
GAGCTTAAGGGGTCTCATTTTTGTTACTGTTCCAAAGTACAAAAAAAATAGAAAATACCC
CCAACTCAACCCCACCCCCNACAACATTTCCCCCAACACAAATAATTTTTCCCAAAACCA
CAAACATAAACTGGTCCTGGTATTTCCATAAACAGTGCAGCTAAGAAACAGTTTAGAGAA
AATTTAACAGGATTCAGATTATATCCATTCTGTCCTCTCGGCCCTGAGAGGTAATAATTC
CCATATGGGTCCTAGTCCTCCCCAATGGTTTTCCCATCTCTGATGGAAGAGTCCTTTTAC
AAAGTGGTGTTTGGCTACTGCTTTAGAGATCCTCCTGTGCCTTCTTCTTCTTCTTGGGTT
TTTCTTCTTGAATTACAGGGGGGTTTGTAGCTTCTCTTTGTAGATAGCTAATAAAATCAC
TTAATTCACGGCCACCTTCATATTTCTTTGGATTTAGCTTCTTGTTGGCTGGAGAGAAGT
ATATGGTAGGAAAACCTCTGACTTCATATGGAGAAGGCACATCATTGGCTGTGGCATCCA
TCTTGGCTATGACGATATTTGGGTCTTTGCTGAGCTTCTCGCCAAGTTCTTTATACTTGG
GCTCCAGGTTCTTACAATGACCACACCAAGGGGCATAAAATTCAATCAGCACATCTTTAT
TTTCATTATTCACTATTTCATCAAAATTCTCTGCTACCACTACCTTCACAGGCCCATCAT
TGCTCTCTGGGATAGGTTCAGACTTCAGGTATCTCTTCAGATTGCCATCAAAGTAATCCT
GCAGGGACCTCTNCAGAGCCTTNCCATCACGCGAGAACTCCTCCTGCATGACAAACTTTT
CTTCCTTAGCAGTTCTGATAAGCACAACAGGGAATCTCTTCAGCAGTGCTCTTCAAGCCA
AAATCAGAAAGTTCATGGCTTAAGGGGTTTGCGC
Restriction Sites NotI-NotI     
ACCN NM_005313
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005313.4, NP_005304.3
RefSeq Size 3060 bp
RefSeq ORF 1518 bp
Locus ID 2923
UniProt ID P30101
Domains thiored
Protein Families Druggable Genome
Protein Pathways Antigen processing and presentation
Gene Summary This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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