ERp57 (PDIA3) (NM_005313) Human Untagged Clone
CAT#: SC116818
PDIA3 (untagged)-Human protein disulfide isomerase family A, member 3 (PDIA3)
CNY 3,768.00
Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HEL-S-93n; HEL-S-269; HsT17083; P58; PI-PLC |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116818 sequence for NM_005313 edited (data generated by NextGen Sequencing)
ATGCGCCTCCGCCGCCTAGCGCTGTTCCCGGGTGTGGCGCTGCTTCTTGCCGCGGCCCGCCTCGCCGCTG CCTCCGACGTGCTAGAACTCACGGACGACAACTTCGAGAGTCGCATCTCCGACACGGGCTCTGCGGGCCT CATGCTCGTCGAGTTCTTCGCTCCCTGGTGTGGACACTGCAAGAGACTTGCACCTGAGTATGAAGCTGCA GCTACCAGATTAAAAGGAATAGTCCCATTAGCAAAGGTTGATTGCACTGCCAACACTAACACCTGTAATA AATATGGAGTCAGTGGATATCCAACCCTGAAGATATTTAGAGATGGTGAAGAAGCAGGTGCTTATGATGG ACCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAGCAGGCAGGACCAGCTTCAGTGCCTCTCAGG ACTGAGGAAGAATTTAAGAAATTCATTAGTGATAAAGATGCCTCTATAGTAGGTTTTTTCGATGATTCAT TCAGTGAGGCTCACTCCGAGTTCCTAAAAGCAGCCAGCAACTTGAGGGATAACTACCGATTTGCACATAC GAATGTTGAGTCTCTGGTGAACGAGTATGATGATAATGGAGAGGGTATCATCTTATTTCGTCCTTCACAT CTCACTAACAAGTTTGAGGACAAGACTGTGGCATATACAGAGCAAAAAATGACCAGTGGCAAAATTAAAA AGTTTATCCAGGAAAACATTTTTGGTATCTGCCCTCACATGACAGAAGACAATAAAGATTTGATACAGGG CAAGGACTTACTTATTGCTTACTATGATGTGGACTATGAAAAGAACGCTAAAGGTTCCAACTACTGGAGA AACAGGGTAATGATGGTGGCAAAGAAATTCCTGGATGCTGGGCACAAACTCAACTTTGCTGTAGCTAGCC GCAAAACCTTTAGCCATGAACTTTCTGATTTTGGCTTGGAGAGCACTGCTGGAGAGATTCCTGTTGTTGC TATCAGAACTGCTAAAGGAGAGAAGTTTGTCATGCAGGAGGAGTTCTCGCGTGATGGGAAGGCTCTGGAG AGGTTCCTGCAGGATTACTTTGATGGCAATCTGAAGAGATACCTGAAGTCTGAACCTATCCCAGAGAGCA ATGATGGGCCTGTGAAGGTAGTGGTAGCAGAGAATTTTGATGAAATAGTGAATAATGAAAATAAAGATGT GCTGATTGAATTTTATGCCCCTTGGTGTGGTCATTGTAAGAACCTGGAGCCCAAGTATAAAGAACTTGGC GAGAAGCTCAGCAAAGACCCAAATATCGTCATAGCCAAGATGGATGCCACAGCCAATGATGTGCCTTCTC CATATGAAGTCAGAGGTTTTCCTACCATATACTTCTCTCCAGCCAACAAGAAGCTAAATCCAAAGAAATA TGAAGGTGGCCGTGAATTAAGTGATTTTATTAGCTATCTACAAAGAGAAGCTACAAACCCCCCTGTAATT CAAGAAGAAAAACCCAAGAAGAAGAAGAAGGCACAGGAGGATCTCTAA Clone variation with respect to NM_005313.4 46 c=>n;47 t=>n;48 t=>n;49 g=>n;50 c=>n;51 c=>n;52 g=>n;53 c=>n;162 c=>t;1224 c=>t >OriGene 5' read for NM_005313 unedited
CGGTGCAGATTTGTATACGACTCACTATAGGCGGCCGCGCAATCGGCACGAGGGCGGGTT AGTGGTCGCGCGCCCGACCTCCGCAGTCCCAGCCGAGCCGCGACCCTTCCGGCCGTCCCC ACCCCACCTCGCCGCCATGCGCCTCCGCCGCCTAGCGCTGTTCCCGGGTGTGGCGCTGCT TCTTGCCGCGGCCCGCCTCGCCGCTGCCTCCGACGTGCTAGAACTCACGGACGACAACTT CGAGAGTCGCATCTCCGACACGGGCTCTGCGGGCCTCATGCTCGTCGAGTTCTTCGCTCC CTGGTGTGGACACTGCAAGAGACTTGCACCTGAGTATGAAGCTGCAGCTACCAGATTAAA AGGAATAGTCCCATTAGCAAAGGTTGATTGCACTGCCAACACTAACACCTGTAATAAATA TGGAGTCAGTGGATATCCAACCCTGAAGATATTTAGAGATGGTGAAGAAGCAGGTGCTTA TGATGGACCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAGCAGGCAGGACCAGC TTCAGTGCCTCTCAGGACTGAGGAAGAATTTAAGAAATTCATTAGTGATAAAGATGCCTC TATAGTAGGTTTTTTCGATGATTCATTCAGTGAGGCTCACTCCGAGTTCCTAGAAGCAGC CAGCAACTTGAGGGATAACTACCGATTTGCACATACCAATGTTGAGTCTCTGGTGAACGA GTATGATGATCATGGAGCAGGGATCATCTTATTTCGTCCTTCACATCTCACTAACCAAGT TTGAAGACAAGACTGTGGCATATACCGAGCCACAAATGACCCGTGGCAACATTTAACAGG TTATCCCGGACAACATTTTTGGTATCTGCCCTCCCTTGACCGAAGACACTTCGATTTGTT CCG >OriGene 3' read for NM_005313 unedited
GAGCTTAAGGGGTCTCATTTTTGTTACTGTTCCAAAGTACAAAAAAAATAGAAAATACCC CCAACTCAACCCCACCCCCNACAACATTTCCCCCAACACAAATAATTTTTCCCAAAACCA CAAACATAAACTGGTCCTGGTATTTCCATAAACAGTGCAGCTAAGAAACAGTTTAGAGAA AATTTAACAGGATTCAGATTATATCCATTCTGTCCTCTCGGCCCTGAGAGGTAATAATTC CCATATGGGTCCTAGTCCTCCCCAATGGTTTTCCCATCTCTGATGGAAGAGTCCTTTTAC AAAGTGGTGTTTGGCTACTGCTTTAGAGATCCTCCTGTGCCTTCTTCTTCTTCTTGGGTT TTTCTTCTTGAATTACAGGGGGGTTTGTAGCTTCTCTTTGTAGATAGCTAATAAAATCAC TTAATTCACGGCCACCTTCATATTTCTTTGGATTTAGCTTCTTGTTGGCTGGAGAGAAGT ATATGGTAGGAAAACCTCTGACTTCATATGGAGAAGGCACATCATTGGCTGTGGCATCCA TCTTGGCTATGACGATATTTGGGTCTTTGCTGAGCTTCTCGCCAAGTTCTTTATACTTGG GCTCCAGGTTCTTACAATGACCACACCAAGGGGCATAAAATTCAATCAGCACATCTTTAT TTTCATTATTCACTATTTCATCAAAATTCTCTGCTACCACTACCTTCACAGGCCCATCAT TGCTCTCTGGGATAGGTTCAGACTTCAGGTATCTCTTCAGATTGCCATCAAAGTAATCCT GCAGGGACCTCTNCAGAGCCTTNCCATCACGCGAGAACTCCTCCTGCATGACAAACTTTT CTTCCTTAGCAGTTCTGATAAGCACAACAGGGAATCTCTTCAGCAGTGCTCTTCAAGCCA AAATCAGAAAGTTCATGGCTTAAGGGGTTTGCGC |
Restriction Sites | NotI-NotI |
ACCN | NM_005313 |
Insert Size | 2000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_005313.4, NP_005304.3 |
RefSeq Size | 3060 bp |
RefSeq ORF | 1518 bp |
Locus ID | 2923 |
UniProt ID | P30101 |
Domains | thiored |
Protein Families | Druggable Genome |
Protein Pathways | Antigen processing and presentation |
Gene Summary | This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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Pro-apoptotic activities of PDI and PDIA3: a role of Bcl-2 protein Bak
,Zhao, G;Lu, H;Li, C;,
J. Biol. Chem.
,PubMed ID 25697356
[PDIA3]
|
Chaperone Properties of Pdia3 Participate in Rapid Membrane Actions of 1a,25-Dihydroxyvitamin D3
,Jiaxuan Chen, Kirill S. Lobachev, Brian J. Grindel, Mary C. Farach-Carson, Sharon L. Hyzy, Khairat B. El-Baradie, Rene Olivares-Navarrete, Maryam Doroudi, Barbara D. Boyan, and Zvi Schwartz,
Mol. Endocrinol., Jul 2013; 27: 1065 - 1077.
,PubMed ID 23660595
[Pdia3]
|
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Other Versions
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RC205940L2 | Lenti ORF clone of Human protein disulfide isomerase family A, member 3 (PDIA3), mGFP tagged |
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RC205940L3 | Lenti ORF clone of Human protein disulfide isomerase family A, member 3 (PDIA3), Myc-DDK-tagged |
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RC205940L4 | Lenti ORF clone of Human protein disulfide isomerase family A, member 3 (PDIA3), mGFP tagged |
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RG205940 | PDIA3 (tGFP-tagged) - Human protein disulfide isomerase family A, member 3 (PDIA3) |
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