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AMPK beta 2 (PRKAB2) (NM_005399) Human Untagged Clone
CAT＃: SC116791
PRKAB2 (untagged)-Human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2)
CNY 3,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_005399 edited
ATTCGGCACCAGCTTTATTGGTGGCCTGATCTGCAGAACAGCCATCACATCAGTGGCCCT TGGAGGAGGGAGCGCATCGCCCGAGGTGGTCCCCGACGAGCTGCAGCCATGGGAAACACC ACCAGCGACCGGGTGTCCGGGGAGCGCCACGGCGCCAAGGCTGCACGCTCCGAGGGCGCA GGCGGCCATGCCCCGGGGAAGGAGCACAAGATCATGGTGGGGAGTACGGACGACCCCAGC GTGTTCAGCCTCCCTGACTCCAAGCTCCCTGGGGACAAAGAGTTTGTATCATGGCAGCAG GATTTGGAGGACTCCGTAAAGCCCACACAGCAGGCCCGGCCCACTGTTATCCGCTGGTCT GAAGGAGGCAAGGAGGTCTTCATCTCTGGGTCCTTCAACAATTGGAGCACCAAGATTCCA CTGATTAAGAGCCATAATGACTTTGTTGCCATCCTGGACCTCCCTGAGGGAGAGCACCAA TACAAGTTCTTTGTGGATGGACAGTGGGTTCATGATCCATCAGAGCCTGTGGTTACCAGT CAGCTTGGCACAATTAACAATTTGATCCATGTCAAGAAATCTGATTTTGAGGTGTTCGAT GCTTTAAAGTTAGATTCTATGGAAAGTTCTGAGACATCTTGTAGAGACCTTTCCAGCTCA CCCCCAGGGCCTTATGGTCAAGAAATGTATGCGTTTCGATCTGAGGAAAGATTCAAATCC CCACCCATCCTTCCTCCTCATCTACTTCAAGTTATTCTTAACAAAGACACTAATATTTCT TGTGACCCAGCCTTACTCCCTGAGCCCAACCATGTTATGCTGAACCATCTCTATGCATTG TCCATTAAGGACAGTGTGATGGTCCTTAGCGCAACCCATCGCTACAAGAAGAAGTATGTT ACTACTCTGCTATACAAGCCCATTTGAAGGGATCCCTTCTTGCCTCTAAGGATTCAGGAG AAGCATCTCCCTTGCATTTCTGGACTGAACCAGTCTTACCTGAGACTGGAAGGCTGATTT GCTTTGAGGCTGATATGTGTGTTTCAGAGCCTCTGAGTAGGATGCTCTGCTTTTGCATTT GATTGCAGATGAGAGCTTTATGAGTTCACGGAATTTATTTTAAGAAAAAAAA >OriGene 5' read for NM_005399 unedited
TGCAGAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACCAGCTTTATT GGTGGCCTGATCTGCAGAACAGCCATCACATCAGTGGCCCTTGGAGGAGGGAGCGCATCG CCCGAGGTGGTCCCCGACGAGCTGCAGCCATGGGAAACACCACCAGCGACCGGGTGTCCG GGGAGCGCCACGGCGCCAAGGCTGCACGCTCCGAGGGCGCAGGCGGCCATGCCCCGGGGA AGGAGCACAAGATCATGGTGGGGAGTACGGACGACCCCAGCGTGTTCAGCCTCCCTGACT CCAAGCTCCCTGGGGACAAAGAGTTTGTATCATGGCAGCAGGATTTGGAGGACTCCGTAA AGCCCACACAGCAGGCCCGGCCCACTGTTATCCGCTGGTCTGAAGGAGGCAAGGAGGTCT TCATCTCTGGGTCCTTCAACAATTGGAGCACCAAGATTCCACTGATTAAGAGCCATAATG ACTTTGTTGCCATCCTGGACCTCCCTGAGGGAGAGCACCAATACAAGTTCTTTGTGGATG GACAGTGGGTTCATGATCCATCAGAGCCTGTGGTTACCAGTCAGCTTGGCACAATTAACA ATTTGATCCATGTCAAGAAATCTGATTTTGAGGTGTTCGATGCTTTAAAGTTAGATTCTA TGGAAAGTTCTGAGACATCTTGTAGAGACCTTTCCAGCTCACCCCCAGGGCCTTATGGTC AAGAAATGTATGCGTTTCGATCTGAGGAAAGATTCAAATNCCCACCCATCCTTTCTNCTC ATCTACTTCAAGTTATTCTTAACAAAACACTAATTTNTCTTGTGACCCAGCCTTACTCCC TGAGCCCAAACCATGTATGCTGAACCATCTCTATGCATTGTCCATTAGGNACAGTGTGAT GGTTCTTTAGCGCACCATCGCTACAGAAGAAGTATGTACTACTCTGCTTACAAGCCATTN GAAGGATCCCTTCTTGCT >OriGene 3' read for NM_005399 unedited
NNCCGGTTTATCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTT TGCTGTATAATACTTTTATTAAAGTTTTATGGGAAAAAACCCACAACATNTTCTGTATGT ATCATATCATATAATTTCCAGGTGTGTCAGAGTCCAGTGACAAATTACGTTTGAAGAACA GCTTTAAAATCCACTCTTGTCTGCCACTCCTTTATGAAATATTGAAAAGCAGCTCTGTAC CTCAGCATTAAAGGTTACAAAAAGCACCATTAAAAAGAGGACTCACATATTTAATTCCCT TCAAAGTAGTCTTATCTCTCCTTTCTGACAGACACAGAGCTGCACTCATTCGAAATGCTG CCGTGTAATACCAACCAACATGCTCCAATGTACCAAAATTCAAACAAGTCTCATTTATGA GACCCGTGTAGTGATTAGGACTAACCAACAACAGTGAGCTTTGGCTGTCCTAAAACCACA AGCTGGGTTAGGTAAGTGTTTTGTACAGGGGAACAGAAAAGAAGTAAGATCATCAATACC TAATATATGACTTAATGTAGGTATCCCTTGACCATTTAACCTGACACCTACAAAATCCAT AGTGTTTAGTATTTCACACCAATCTTACTGCAAAAGTGCTGTGCTACATACAATCAATTC AAGTTAACTCCAACTAATATTGGAACTAATAAAAAATTAATTTGTAACAAAGCTTATTCA CTTCAAAGCACAACTGAGAAAAGCAAATCCAGACAAAGCCCAAACTAGAATCCTAGAGCG ATTCCATTGCTAAGAATTGGACTCTACAAGTAATAGACTTGAGTTNTTCTCTTTTTTCCT TCCTTCTTTAATTTAATTTTGCTTTTACTCTAAAGTTTGGGTTCATGCAGAATCCAGTCC TTCTATCCCATGTGATTGCAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005399 |
| Insert Size | 5270 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005399.3, NP_005390.1 |
| RefSeq Size | 5431 bp |
| RefSeq ORF | 819 bp |
| Locus ID | 5565 |
| UniProt ID | O43741 |
| Domains | AMPKBI |
| Protein Families | Druggable Genome |
| Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway |
| Gene Summary | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) encodes a functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208766 | PRKAB2 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2) |
CNY 3,600.00 |
|
| RC208766L1 | Lenti ORF clone of Human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC208766L2 | Lenti ORF clone of Human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2), mGFP tagged |
CNY 6,000.00 |
|
| RC208766L3 | Lenti ORF clone of Human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208766L4 | Lenti ORF clone of Human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2), mGFP tagged |
CNY 6,000.00 |
|
| RG208766 | PRKAB2 (tGFP-tagged) - Human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2) |
CNY 5,200.00 |
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