HMG4 (HMGB3) (NM_005342) Human Untagged Clone

CAT#: SC116765

HMGB3 (untagged)-Human high mobility group box 3 (HMGB3)



  "NM_005342" in other vectors (4)

CNY 3,990.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (3)
Anti-HMGB3 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HMG-2a; HMG-4; HMG2A; HMG4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_005342, the custom clone sequence may differ by one or more nucleotides


ATGGCTAAAGGTGACCCCAAGAAACCAAAGGGCAAGATGTCCGCTTATGCCTTCTTTGTGCAGACATGCA
GAGAAGAACATAAGAAGAAAAACCCAGAGGTCCCTGTCAATTTTGCGGAATTTTCCAAGAAGTGCTCTGA
GAGGTGGAAGACGATGTCCGGGAAAGAGAAATCTAAATTTGATGAAATGGCAAAGGCAGATAAAGTGCGC
TATGATCGGGAAATGAAGGATTATGGACCAGCTAAGGGAGGCAAGAAGAAGAAGGATCCTAATGCTCCCA
AAAGGCCACCGTCTGGATTCTTCCTGTTCTGTTCAGAATTCCGCCCCAAGATCAAATCCACAAACCCCGG
CATCTCTATTGGAGACGTGGCAAAAAAGCTGGGTGAGATGTGGAATAATTTAAATGACAGTGAAAAGCAG
CCTTACATCACTAAGGCGGCAAAGCTGAAGGAGAAGTATGAGAAGGATGTTGCTGACTATAAGTCGAAAG
GAAAGTTTGATGGTGCAAAGGGTCCTGCTAAAGTTGCCCGGAAAAAGGTGGAAGAGGAAGATGAAGAAGA
GGAGGAGGAAGAAGAGGAGGAGGAGGAGGAGGAGGATGAATAA


>OriGene 5' read for NM_005342 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGCCAATCAGGCGG
CTCTCGTGGAGGCAGCTAGCGCGAGGCTGGGGAGCGCTGAGCCGCGCGTCGTGCCCTGCG
CTGCCCAGACTAGCGAACAATACAGTCAGGATGGCTAAAGGTGACCCCAAGAAACCAAAG
GGCAAGATGTCCGCTTATGCCTTCTTTGTGCAGACATGCAGAGAAGAACATAAGAAGAAA
AACCCAGAGGTCCCTGTCAATTTTGCGGAATTTTCCAAGAAGTGCTCTGAGAGGTGGAAG
ACGATGTCCGGGAAAGAGAAATCTAAATTTGATGAAATGGCAAAGGCAGATAAAGTGCGC
TATGATCGGGAAATGAAGGATTATGGACCAGCTAAGGGAGGCAAGAAGAAGAAGGATCCT
AATGCTCCCAAAAGGCCACCGTCTGGATTCTTCCTGTTCTGTTCAGAATTCCGCCCCAAG
ATCAAATCCACAAACCCCGGCATCTCTATTGGAGACGTGGCAAAAAAGCTGGGTGAGATG
TGGAATAATTTAAATGACAGTGAAAAGCAGCCTTACATCACTAANGCCGGCAAGCTGAAG
GAGAAGTATGAGAAGGATGTTGCTGACTATAAGTCGAAAGGAAAGTTTGATGGTGCAAAG
GGTCCTGCTAAGTTTGCCCCGGAAAAGGTGGAAGAGGAAGATGAAGACGAGGACGAGGAA
TAACAGAGAGGAGGAGGAAGGAGAAGATTGAATAAAAAAACTGTTTATTCTGCCTCCTTG
CGAAAACTAGAGTAAGGGAAGCGCCGAAATTGCACATTTCTTTATTTGAGAGGTGGCTGG
GTGCCTTCATTAGGTTAATTACAAATTTGAACCCGACATATTGGACTCCTCAAAAGCTCT
AAAATTGCAATGGTTAACTGAAAGGCCTGGTGTTTGAACACCCGAACTGATAAGGTGACT
T
>OriGene 3' read for NM_005342 unedited
GAACTAGAGACTGTATATCTTAACTCCAAAACATTTCAGCATTCTAAACATACAAAAAAA
TAACAGAACGTTGCGAATCGTGTTTAAGTACAGGAGGTTCTTGAACTTTCATTGATGCAG
TGACTCTTTGCTTTGCTGACAATGAAGAGTTCTATAGTTTGTTTAAAAACAAACAGTTTA
AAAACTACCGTACTTAAAAAAAAAAATATTCTCATGCCAGCTGACCCCCCTTTGTCCACA
GCTAAGATGGCAGTTGAATGCTATGTCACTATGTTCTGAAACAAGACAACCTGTAGCTAA
ATGGATGCCCCCTGCTCAGTGAACAGGTCCAGACTTACTCTGCACGCTCTGTGTTACTGT
CTGTCCCAAAACGTATTTTCCTCACATGTTCCACGCCTTTCTATGATCACGAAGGGTTTG
TATCGGTTGTTTTGTTCTTTTCTCACACTATGCATGTATTCTGGGGACAACTCACGATTG
CTAGCCAATAACCATACTTGCCACCACCTTGCCAATTATAAGAAAATGTCACAAACCATT
CCAAACGCCTTTGTCCCGTCAACAGTTATGTGCTCAGTTGTGTGGATTAACACCTTAGTG
CCGTTTCTCCTCGTAAAATGTCTTGGAACATATGCACAACTGTGATACTATAGGTGGGTG
TTTCTAACACCCATGGACACTTAGATGAGAACCATTAGAAACTCCTAGAGATCTTGTCGG
AGGATTCCCCCTGACTCGACCTCGAATGTTGTAATTAAACCTAATGCGGTGCATCTCGCA
CTTTCAACATAAGAGAAGTGTCCATTACCGTGCTCCCGGACTTCATTATTTCTCTCTGAG
ACAGAAACTAGTAACGTTATTGTTCTCTTTGCACGTCCTACTGCAGTTTTACTGATTCGC
TACTTTCTCCTACTGCTACATACCTTTCTTCGGCGACT
Restriction Sites NotI-NotI     
ACCN NM_005342
Insert Size 1670 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005342.2, NP_005333.2
RefSeq Size 3556 bp
RefSeq ORF 603 bp
Locus ID 3149
UniProt ID O15347
Domains HMG
Protein Families Transcription Factors
Gene Summary This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Transcript Variant: This variant (2) differs in the 5' UTR, compared to vaiant 1. Variants 1, 2, and 3 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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