TFIIE alpha (GTF2E1) (NM_005513) Human Untagged Clone

Name: TFIIE alpha (GTF2E1) (NM_005513) Human Untagged Clone
SKU: SC116700
Price: 3656.00

CAT＃: SC116700

GTF2E1 (untagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1)

Datasheet

SDS

"NM_005513" in other vectors (6)

CNY 3,656.00

CNY 7,030.00

货期*

现货

规格

Product images

经常一起买 (4)

TA376847

GTF2E1 Rabbit polyclonal Antibody

CNY 1,999.00
CNY 3,280.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FE; TF2E1; TFIIE-A
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC116700 sequence for NM_005513 edited (data generated by NextGen Sequencing) ATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAAGTAT GTGATCCGGGGATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAGGAAC TCCTGTGTGAAAGAGGAGGATATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACTTCGA TCAGTTTTGAATAATTTAAAGGGAGACAAGTTTATCAAATGCAGAATGAGGGTAGAGACT GCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCATCAATTATCGTACTCTTGTT AATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGATGAGAGAGAT TCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTTAGAA GCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATACAGAG GTAGAAGAGGATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAGGTTT AATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTGAACTTGGCC TATGAAATACTTGAGCCAGAACCCACAGAAATCCCAGCCCTGAAACAGAGCAAGGACCAT GCAGCAACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGCACCACCGGGAAGCATGGGCC ACCAAAGGTCCTTCCTATGAAGACTTATACACTCAGAATGTTGTCATTAACATGGATGAC CAAGAAGATCTTCATCGAGCCTCACTGGAAGGGAAATCTGCCAAAGAGAGGCCTATTTGG TTGAGAGAAAGCACTGTCCAAGGGGCATATGGTTCTGAAGATATGAAAGAAGGGGGCATA GATATGGACGCATTTCAGGAGCGTGAGGAAGGCCATGCTGGGCCTGATGACAACGAAGAG GTCATGCGAGCACTGCTCATTCACGAGAAAAAGACTTCCTCTGCCATGGCTGGTTCAGTG GGGGCAGCTGCTCCAGTGACCGCTGCCAATGGCAGTGACTCAGAAAGCGAGACCAGTGAG TCAGATGATGATTCTCCACCCCGTCCGGCAGCTGTGGCTGTGCATAAACGAGAAGAGGAT GAAGAGGAAGATGACGAGTTTGAAGAAGTAGCAGATGACCCCATTGTCATGGTGGCTGGC CGTCCGTTCTCCTACAGTGAAGTGAGCCAACGGCCAGAGCTAGTGGCCCAGATGACACCA GAAGAAAAGGAAGCATATATAGCAATGGGACAACGCATGTTTGAGGACCTCTTTGAGTGA Clone variation with respect to NM_005513.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_005513 unedited GGGTCACATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGCTGGGGG CAGCTGTAGTGGGAGTGTTCCAGGATTCGCCTTGTATATTTAAAGTTGGAGTTCGTTGCT AAAGATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAA GTATGTGATCCGGGGATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAG GAACTCCTGTGTGAAAGAGGAGGATATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACT TCGATCAGTTTTGAATAATTTAAAGGGAGACAAGTTTATCAAATGCAGAATGAGGGTAGA GACTGCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCATCAATTATCGTACTCT TGTTAATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGATGAGAG AGATTCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTT AGAAGCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATAC AGAGGTAGAAGAGGATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAG GTTTAATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTGAACTT GGCCTATGAAATACTTGAGCCAGAACCCACAGAAATCCCAGCCCTGAAACAGAGCAAGGA CCATGCAGNCACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGCACCACCGGGGAGCATG GGCCACCAAAGGTCCTTTCTATGAAGACTTATACACTCAGAATGTTGTCATTTACATGGA TGACCAAGAAGATCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_005513 unedited GTATTTGTCACATTCAGTCTTAACACAACCCGGTAAATGAAACATCACTCCATACAAGGC TCATATATTCTTTACATGTGTACTATAAAATGATAAATACTTAAAAATTACAATGCAAGG AAAAATATTGTAAGATATACAATTGTTACAGACCCTTGTGTGAAAGTTCTTTATTTTTAA AGTTCTGCCTTAGGGAAAATACCATGCTACATATCAGAACCCAAAATAAACTGTTTCCTG AGGTTACAATGCTCAATGGTATACTGGATGCACCCTAAAATACTGTGTCTGACAAAAATT AAAAGAGAGACAGCCTTCAATGATGTTTAGAAAGAGGGAGAGTGACAGAGAGAGAGAAGA GTAATCAATTATTCTGTTGATTTCTGCTGGAATGGCCTCATTAAACAATTTATAGCACTG ACAAGAGGTCTGTACCCAAAGGGAACAAAACCAGGACCAGTCTCAGCTTTACATAAAGGA TTTCCAAATCAGACTAACTGACACAGTTCTAAGGCACTGTTACCAATGCTTGTCACTCTT CAACCCTTCACAGAGACTTCTTGTCTTACGTCCATGTTTTTCTAATTTCTTCCTAAGGGT TTACCAATTATACTTCTGTTTTCTATCATCCTAAATCCCAACCAAAAGACATTAANGCAT ACAATAAAATCTACCAGTCCTGGTCAGTTCTAAAGACACAAAATCTGGTTTAGACATTAT TCATAGACTCAGTTTATNTTTCCCCTTTCAACACGCTCCCATTNCCCTTCATATTGATCC ATCTAATTTTGAGGAATGCTCAATTCTAAGCAAAATTCAGGAATTTTTCCCCATAAGGAT TAGACTGTGGAAACTGTTTTGGTGGGCCCCATAAAAATTGNTGGAAGCCTGCTTGAAAAA TAAAGAGGGGACAGGATTTTGGACCTCTGGTTAACCAAGGTGGAAGGTTACCTTGAACTC CCTCCTTCTTTAAGGGAAAATTTTTTTTTAAGAGAACAAATAAAA
Restriction Sites	NotI-NotI
ACCN	NM_005513
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_005513.1, NP_005504.1
RefSeq Size	2969 bp
RefSeq ORF	1320 bp
Locus ID	2960
UniProt ID	P29083
Domains	TFIIE
Protein Families	Transcription Factors
Protein Pathways	Basal transcription factors
Gene Summary	Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC218763	GTF2E1 (Myc-DDK-tagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 3,990.00
RC218763L1	Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC218763L2	Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC218763L3	Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC218763L4	Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG218763	GTF2E1 (tGFP-tagged) - Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,256.00

其它TFIIE alpha产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies