TFIIE alpha (GTF2E1) (NM_005513) Human Untagged Clone
CAT#: SC116700
GTF2E1 (untagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1)
CNY 3,656.00
CNY 7,030.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | FE; TF2E1; TFIIE-A |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116700 sequence for NM_005513 edited (data generated by NextGen Sequencing)
ATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAAGTAT GTGATCCGGGGATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAGGAAC TCCTGTGTGAAAGAGGAGGATATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACTTCGA TCAGTTTTGAATAATTTAAAGGGAGACAAGTTTATCAAATGCAGAATGAGGGTAGAGACT GCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCATCAATTATCGTACTCTTGTT AATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGATGAGAGAGAT TCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTTAGAA GCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATACAGAG GTAGAAGAGGATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAGGTTT AATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTGAACTTGGCC TATGAAATACTTGAGCCAGAACCCACAGAAATCCCAGCCCTGAAACAGAGCAAGGACCAT GCAGCAACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGCACCACCGGGAAGCATGGGCC ACCAAAGGTCCTTCCTATGAAGACTTATACACTCAGAATGTTGTCATTAACATGGATGAC CAAGAAGATCTTCATCGAGCCTCACTGGAAGGGAAATCTGCCAAAGAGAGGCCTATTTGG TTGAGAGAAAGCACTGTCCAAGGGGCATATGGTTCTGAAGATATGAAAGAAGGGGGCATA GATATGGACGCATTTCAGGAGCGTGAGGAAGGCCATGCTGGGCCTGATGACAACGAAGAG GTCATGCGAGCACTGCTCATTCACGAGAAAAAGACTTCCTCTGCCATGGCTGGTTCAGTG GGGGCAGCTGCTCCAGTGACCGCTGCCAATGGCAGTGACTCAGAAAGCGAGACCAGTGAG TCAGATGATGATTCTCCACCCCGTCCGGCAGCTGTGGCTGTGCATAAACGAGAAGAGGAT GAAGAGGAAGATGACGAGTTTGAAGAAGTAGCAGATGACCCCATTGTCATGGTGGCTGGC CGTCCGTTCTCCTACAGTGAAGTGAGCCAACGGCCAGAGCTAGTGGCCCAGATGACACCA GAAGAAAAGGAAGCATATATAGCAATGGGACAACGCATGTTTGAGGACCTCTTTGAGTGA Clone variation with respect to NM_005513.2 >OriGene 5' read for NM_005513 unedited
GGGTCACATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGCTGGGGG CAGCTGTAGTGGGAGTGTTCCAGGATTCGCCTTGTATATTTAAAGTTGGAGTTCGTTGCT AAAGATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAA GTATGTGATCCGGGGATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAG GAACTCCTGTGTGAAAGAGGAGGATATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACT TCGATCAGTTTTGAATAATTTAAAGGGAGACAAGTTTATCAAATGCAGAATGAGGGTAGA GACTGCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCATCAATTATCGTACTCT TGTTAATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGATGAGAG AGATTCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTT AGAAGCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATAC AGAGGTAGAAGAGGATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAG GTTTAATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTGAACTT GGCCTATGAAATACTTGAGCCAGAACCCACAGAAATCCCAGCCCTGAAACAGAGCAAGGA CCATGCAGNCACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGCACCACCGGGGAGCATG GGCCACCAAAGGTCCTTTCTATGAAGACTTATACACTCAGAATGTTGTCATTTACATGGA TGACCAAGAAGATCT >OriGene 3' read for NM_005513 unedited
GTATTTGTCACATTCAGTCTTAACACAACCCGGTAAATGAAACATCACTCCATACAAGGC TCATATATTCTTTACATGTGTACTATAAAATGATAAATACTTAAAAATTACAATGCAAGG AAAAATATTGTAAGATATACAATTGTTACAGACCCTTGTGTGAAAGTTCTTTATTTTTAA AGTTCTGCCTTAGGGAAAATACCATGCTACATATCAGAACCCAAAATAAACTGTTTCCTG AGGTTACAATGCTCAATGGTATACTGGATGCACCCTAAAATACTGTGTCTGACAAAAATT AAAAGAGAGACAGCCTTCAATGATGTTTAGAAAGAGGGAGAGTGACAGAGAGAGAGAAGA GTAATCAATTATTCTGTTGATTTCTGCTGGAATGGCCTCATTAAACAATTTATAGCACTG ACAAGAGGTCTGTACCCAAAGGGAACAAAACCAGGACCAGTCTCAGCTTTACATAAAGGA TTTCCAAATCAGACTAACTGACACAGTTCTAAGGCACTGTTACCAATGCTTGTCACTCTT CAACCCTTCACAGAGACTTCTTGTCTTACGTCCATGTTTTTCTAATTTCTTCCTAAGGGT TTACCAATTATACTTCTGTTTTCTATCATCCTAAATCCCAACCAAAAGACATTAANGCAT ACAATAAAATCTACCAGTCCTGGTCAGTTCTAAAGACACAAAATCTGGTTTAGACATTAT TCATAGACTCAGTTTATNTTTCCCCTTTCAACACGCTCCCATTNCCCTTCATATTGATCC ATCTAATTTTGAGGAATGCTCAATTCTAAGCAAAATTCAGGAATTTTTCCCCATAAGGAT TAGACTGTGGAAACTGTTTTGGTGGGCCCCATAAAAATTGNTGGAAGCCTGCTTGAAAAA TAAAGAGGGGACAGGATTTTGGACCTCTGGTTAACCAAGGTGGAAGGTTACCTTGAACTC CCTCCTTCTTTAAGGGAAAATTTTTTTTTAAGAGAACAAATAAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_005513 |
Insert Size | 3000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005513.1, NP_005504.1 |
RefSeq Size | 2969 bp |
RefSeq ORF | 1320 bp |
Locus ID | 2960 |
UniProt ID | P29083 |
Domains | TFIIE |
Protein Families | Transcription Factors |
Protein Pathways | Basal transcription factors |
Gene Summary | Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218763 | GTF2E1 (Myc-DDK-tagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1) |
CNY 3,656.00 |
|
RC218763L1 | Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC218763L2 | Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), mGFP tagged |
CNY 5,890.00 |
|
RC218763L3 | Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC218763L4 | Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), mGFP tagged |
CNY 5,890.00 |
|
RG218763 | GTF2E1 (tGFP-tagged) - Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1) |
CNY 5,256.00 |
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