United States
Germany
Japan
United Kingdom
China
Lamin B1 (LMNB1) (NM_005573) Human Untagged Clone
CAT＃: SC116661
LMNB1 (untagged)-Human lamin B1 (LMNB1), transcript variant 1
CNY 6,552.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADLD; LMN; LMN2; LMNB; MCPH26 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116661 sequence for NM_005573 edited (data generated by NextGen Sequencing)
ATGGCGACTGCGACCCCCGTGCCGCCGCGGATGGGCAGCCGCGCTGGCGGCCCCACCACG CCGCTGAGCCCCACGCGCCTGTCGCGGCTCCAGGAGAAGGAGGAGCTGCGCGAGCTCAAT GACCGGCTGGCGGTGTACATCGACAAGGTGCGCAGCCTGGAGACGGAGAACAGCGCGCTG CAGCTGCAGGTGACGGAGCGCGAGGAGGTGCGCGGCCGTGAGCTCACCGGCCTCAAGGCG CTCTACGAGACCGAGCTGGCCGACGCGCGACGCGCGCTCGACGACACGGCCCGCGAGCGC GCCAAGCTGCAGATCGAGCTGGGCAAGTGCAAGGCGGAACACGACCAGCTGCTCCTCAAC TATGCTAAGAAGGAATCTGATCTTAATGGCGCCCAGATCAAGCTTCGAGAATATGAAGCA GCACTGAATTCGAAAGATGCAGCTCTTGCTACTGCACTTGGTGACAAAAAAAGTTTAGAG GGAGATTTGGAGGATCTGAAGGATCAGATTGCCCAGTTGGAAGCCTCCTTAGCTGCAGCC AAAAAACAGTTAGCAGATGAAACTTTACTTAAAGTAGATTTGGAGAATCGTTGTCAGAGC CTTACTGAGGACTTGGAGTTTCGCAAAAGCATGTATGAAGAGGAGATTAACGAGACCAGA AGGAAGCATGAAACGCGCTTGGTAGAGGTGGATTCTGGGCGTCAAATTGAGTATGAGTAC AAGCTGGCGCAAGCCCTTCATGAGATGAGAGAGCAACATGATGCCCAAGTGAGGCTGTAT AAGGAGGAGCTGGAGCAGACTTACCATGCCAAACTTGAGAATGCCAGACTGTCATCAGAG ATGAATACTTCTACTGTCAACAGTGCCAGGGAAGAACTGATGGAAAGCCGCATGAGAATT GAGAGCCTTTCATCCCAGCTTTCTAATCTACAGAAAGAGTCTAGAGCATGTTTGGAAAGG ATTCAAGAATTAGAGGACTTGCTTGCTAAAGAAAAAGACAACTCTCGTCGCATGCTGACA GACAAAGAGAGAGAGATGGCGGAAATAAGGGATCAAATGCAGCAACAGCTGAATGACTAT GAACAGCTTCTTGATGTAAAGTTAGCCCTGGACATGGAAATCAGTGCTTACAGGAAACTC TTAGAAGGCGAAGAAGAGAGGTTGAAGCTGTCTCCAAGCCCTTCTTCCCGTGTGACAGTA TCCCGAGCATCCTCAAGTCGTAGTGTACGTACAACTAGAGGAAAGCGGAAGAGGGTTGAT GTGGAAGAATCAGAGGCGAGTAGTAGTGTTAGCATCTCTCATTCCGCCTCAGCCACTGGA AATGTTTGCATCGAAGAAATTGATGTTGATGGGAAATTTATCCGCTTGAAGAACACTTCT GAACAGGATCAACCAATGGGAGGCTGGGAGATGATCAGAAAAATTGGAGACACATCAGTC AGTTATAAATATACCTCAAGATATGTGCTGAAGGCAGGCCAGACTGTTACAATTTGGGCT GCAAACGCTGGTGTCACAGCCAGCCCCCCAACTGACCTCATCTGGAAGAACCAGAACTCG TGGGGCACTGGCGAAGATGTGAAGGTTATATTGAAAAATTCTCAGGGAGAGGAGGTTGCT CAAAGAAGTACAGTCTTTAAAACAACCATACCTGAAGAAGAGGAGGAGGAGGAAGAAGCA GCTGGAGTGGTTGTTGAGGAAGAACTTTTCCACCAGCAGGGAACCCCAAGAGCATCCAAT AGAAGCTGTGCAATTATGTAA Clone variation with respect to NM_005573.3 >OriGene 5' read for NM_005573 unedited
NGGTGTCAACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGGAG ACGGCGGCGGCGCGAACCCTGCTGGGCCTCCAGTCACCCTCGTCTTGCATTTTCCCGCGT GCGTGTGTGAGTGGGTGTGTGTGTTTTCTTACAAAGGGTATTTCGCGATCGATCGATTGA TTCGTAGTTCCCCCCCGCGCGCCTTTGCCCTTTGTGCTGTAATCGAGCTCCCGCCATCCC AGGTGCTTCTCCGTTCCTCTAAACGCCAGCGTCTGGACGTGAGCGCAGGTCGCCGGTTTG TGCCTTCGGTCCCCGCTTCGCCCCCTGCCGTCCCCTCCTTATCACGGTCCCGCTCGCGGC CTCGCCGCCCCGCTGTCTCCGCCGCCCGCCATGGCGACTGCGACCCCCGTGCCGCCGCGG ATGGGCAGCCGCGCTGGCGGCCCCACCACGCCGCTGAGCCCCACGCGCCTGTCGCGGCTC CAGGAGAAGGAGGAGCTGCGCGAGCTCAATGACCGGCTGGCGGTGTACATCGACAAGGTG CGCAGCCTGGAGACGGAGAACAGCGCGCTGCAGCTGCAGGTGACGGAGCGCGAGGAGGTG CGCGGCCGTGAGCTCACCGGCCTCAAGGCGCTCTACGAGACCGAGCTGGCCGACGCGCGA CGCGCGCTCGACGACACGGCCCGCGAGCGCGCCAAGCTGCAGATCGAGCTGNGCAAGTGC AAGGCGGAACACGACCAGCTGCTCCTCAACTATGCTAAGAAGGAATCTGATCTTAATGGC GCCCAGATCAAGCTTCGAGAATATGAGCAGCACTGAATTCGAAGATGCAGCTCTTGCTAC TGCACTTGGTGACAAAAAAAGTTANAGGGAGATTNGGNAGATCTGAANGATCAGATTGCC CCATTTGGAAGCCTTCTTAGCTGCAGCCAAAAACAGTTT >OriGene 3' read for NM_005573 unedited
GCTATGGACCGCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTC CAATATTAAAAGAGCTTTTTTATTTGTTAATGTCACATAAGAAATTTAAGCAAGTTACAC TATCTTAAAAAACACAACGAATGCATTTTAATAGAGAAACCCTTCCCTCCCTCCACCTCC CTCCCCCACCCTCCTCATGAATTAAGAATCTAAGAGAAGAAGTACCCATAAAACCAAGTT TTGTGGAATCCATCATCCAGAGTGCTTACATGGGGATTAGGTTAATATTGCCTTCTTACA AAATTTCTATTTTAAAAAAAATTATAACCTTGATTGCTTATTACAAAAAAATTCAGTCCA AAAGTTCAATATCTTGAAAAATGCTTTTCCCCTCCCTCACAGCACCGTTTTATATATAGC AGAGAATAATGAAGAGATTGCTAGTCTAGATGGGGCAATCTTCAAATTACACCACGACGC ACAGTGGTTTATTTACCCTCCCCTCCTCCTAAGAACTTAAGAAAAAACAAAATACCCACC TCCAAAAAGAGTCAAAAAATTTGAGGAACCCCTTCCGAACCGTCCCCGTTATTAAGTGCA GTGGCAATAATTCACACCTTGCAACAAAGTGTGGGGCCTTGATTTCTTTTTACAAAACTT TCCGGGGCAAAAAGAGAATTAGGGCCATCAGATTTCGCACTTACTAAATCCCCTGAAGGA GAAATTCGAAAAATTCTCGGGCTCTAGAAAGGCCCACCCCTGTAGACGGGGAAGGGAAAC CCACCCTTCTTTATTTGAGAAAAGCCGGTGGAAAATTACGGGCGGGACAGATTTCTCTGG GGGCCGCGGGGGTCTCCGCGCGANGGAAAGGCCGTTTACAAAGACCACGAGAGGGGATGT CCACGCGTCAGCTCCTGAGAGCGGTGTAATGAAGAACGTGTCGCCTGAATAAGCGGCTAA GACGGATTGTGAGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005573 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005573.2, NP_005564.1 |
| RefSeq Size | 2882 bp |
| RefSeq ORF | 1761 bp |
| Locus ID | 4001 |
| UniProt ID | P20700 |
| Domains | IF_tail, filament |
| Gene Summary | This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Lamin B1 sequesters 53BP1 to control its recruitment to DNA damage
,null,
Science Advances
,PubMed ID 34452908
[LMNB1]
|
|
Fibroblasts lacking nuclear lamins do not have nuclear blebs or protrusions but nevertheless have frequent nuclear membrane ruptures
,null,
Proceedings of the National Academy of Sciences of the United States of America
,PubMed ID 30224463
[LMNB1]
|
|
Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes
,Izdebska, M;Gagat, M;Grzanka, A;,
Int. J. Oncol.2017
,PubMed ID 29115590
[LMNB1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201604 | LMNB1 (Myc-DDK-tagged)-Human lamin B1 (LMNB1), transcript variant 1 |
CNY 6,544.00 |
|
| RC201604L1 | Lenti-ORF clone of LMNB1 (Myc-DDK-tagged)-Human lamin B1 (LMNB1), transcript variant 1 |
CNY 8,944.00 |
|
| RC201604L2 | Lenti-ORF clone of LMNB1 (mGFP-tagged)-Human lamin B1 (LMNB1), transcript variant 1 |
CNY 5,890.00 |
|
| RC201604L3 | Lenti-ORF clone of LMNB1 (Myc-DDK-tagged)-Human lamin B1 (LMNB1), transcript variant 1 |
CNY 5,890.00 |
|
| RC201604L4 | Lenti-ORF clone of LMNB1 (mGFP-tagged)-Human lamin B1 (LMNB1), transcript variant 1 |
CNY 5,890.00 |
|
| RG201604 | LMNB1 (tGFP-tagged) - Human lamin B1 (LMNB1), transcript variant 1 |
CNY 8,144.00 |
其它Lamin B1产品
