SF3B4 (NM_005850) Human Untagged Clone
CAT#: SC116494
SF3B4 (untagged)-Human splicing factor 3b, subunit 4, 49kDa (SF3B4)
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | AFD1; Hsh49; SAP49; SF3b49 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_005850, the custom clone sequence may differ by one or more nucleotides
ATGGCTGCCGGGCCGATCTCCGAGCGGAATCAGGATGCCACTGTGTACGTGGGGGGCCTGGATGAGAAGG TTAGTGAACCGCTGCTGTGGGAACTGTTTCTCCAGGCTGGACCAGTAGTCAACACCCACATGCCAAAGGA TAGAGTCACTGGCCAGCACCAAGGCTATGGCTTTGTGGAATTCTTGAGTGAGGAAGATGCTGACTATGCC ATTAAGATCATGAACATGATCAAACTCTATGGGAAGCCAATACGGGTGAACAAAGCATCAGCTCACAACA AAAACCTGGATGTAGGGGCCAACATTTTCATTGGGAACCTGGACCCTGAGATTGATGAGAAGTTGCTTTA TGATACTTTCAGCGCCTTTGGGGTCATCTTACAAACCCCCAAAATTATGCGGGACCCTGACACAGGCAAC TCCAAAGGTTATGCCTTTATTAATTTTGCTTCATTTGATGCTTCGGATGCAGCAATTGAAGCCATGAATG GGCAGTACCTCTGTAACCGTCCTATCACCGTATCTTATGCCTTCAAGAAGGACTCCAAGGGTGAGCGCCA TGGCTCAGCAGCCGAACGACTTCTGGCAGCTCAGAACCCGCTCTCCCAGGCTGATCGCCCTCATCAGCTG TTTGCAGATGCACCTCCTCCACCCTCTGCTCCCAATCCTGTGGTATCATCATTGGGGTCTGGGCTTCCTC CACCAGGCATGCCTCCTCCTGGCTCCTTCCCACCCCCAGTGCCACCTCCTGGAGCCCTCCCACCTGGGAT ACCCCCAGCCATGCCCCCACCACCTATGCCTCCTGGGGCTGCAGGACATGGCCCCCCATCGGCAGGAACC CCAGGGGCAGGACATCCTGGTCATGGACACTCACATCCTCACCCATTCCCACCGGGTGGGATGCCCCATC CAGGGATGTCTCAGATGCAGCTTGCACACCATGGCCCTCATGGCTTAGGACATCCCCACGCTGGACCCCC AGGCTCTGGGGGCCAGCCACCGCCCCGACCACCACCTGGAATGCCTCATCCTGGACCTCCTCCAATGGGC ATGCCCCCCCGAGGGCCTCCATTCGGATCTCCCATGGGTCACCCAGGTCCTATGCCTCCGCATGGTATGC GTGGACCTCCTCCACTGATGCCCCCCCATGGATACACTGGCCCTCCACGACCCCCACCCTATGGCTACCA GCGGGGGCCTCTCCCTCCACCCAGACCCACTCCCCGGCCACCAGTTCCCCCTCGAGGCCCACTTCGAGGC CCTCTCCCTCAGTAA >OriGene 5' read for NM_005850 unedited
TATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGCTCTTTCGCCATGGCTGCCG GGCCGATCTCCGAGCGGAATCAGGATGCCACTGTGTACGTGGGGGGCCTGGATGAGAAGG TTAGTGAACCGCTGCTGTGGGAACTGTTTCTCCAGGCTGGACCAGTAGTCAACACCCACA TGCCAAAGGATAGAGTCACTGGCCAGCACCAAGGCTATGGCTTTGTGGAATTCTTGAGTG AGGAAGATGCTGACTATGCCATTAAGATCATGAACATGATCAAACTCTATGGGAAGCCAA TACGGGTGAACAAAGCATCAGCTCACAACAAAAACCTGGATGTAGGGGCCAACATTTTCA TTGGGAACCTGGACCCTGAGATTGATGAGAAGTTGCTTTATGATACTTTCAGCGCCTTTG GGGTCATCTTACAAACCCCCAAAATTATGCGGGACCCTGACACAGGCAACTCCAAAGGTT ATGCCTTTATTAATTTTGCTTCATTTGATGCTTCGGATGCAGCAATTGAAGCCATGAATG GGCAGTACCTCTGTAACCGTCCTATCACCGTATCTTATGCCTTCAAGAAGGACTCCAAGG GTGAGCGCCATGGCTCANCAGCCGAACGACTTCTGGCAGCTCAGAACCCGCTCTCCCAGG CTGATCGCCCTCATCAGCTGNTTGCAGATGCACCTNCTCCCACCTCTGCTCCCAATCCTG TGGTATCATCATTGGGGTCTGGGGCTTCCTCCACCAGGCATGCCTTCTCTTGGGGTCTCC TTCCACCCCCCAGTGCCCCCTTCTTGGAGCCTCTCCACCTTGGGAATACCCCCAGACCAT GCCCCCACCACTTATGTCTCCTGGGGGCTGCAAGAAATGGCCCCCCCATTCGCAAGGAAC CCCAGGGGCAGGGACATCCTTGGNATGGGACACTACAATCCTCACCCATTCCCCCGGGGG GGGAGG >OriGene 3' read for NM_005850 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAACAAGAGTTTAGTTTTATTTT CTCTGTGCATTTGCAAAATACTCAGGACCAACATAAAAAAGAAATACCTCCTGTGGAAAA AGTTACATTAAAAAGGGGAATGGAGTGGGGGTGCTGAAAGGGATTAGTACCTTTGCCCCA AGGAGCTACAGCATCTCTGATTGGTCCAAGGAATAGAAAAGATATTGGGAAAATGTAACA GGAGGAAGGAAAATGTGAATTTACTGAGGGAGAGGGCCTCGAAGTGGGCCTCGAGGGGGA ACTGGTGGCCGGGGAGTGGGTCTGGGTGGAGGGAGAGGCCCCCGCTGGTAGCCATAGGGT GGGGGTCGTGGAGGGCCAGTGTATCCATGGGGGGGCATCAGTGGAGGAGGTCCACGCATA CCATGCGGAGGCATAGGACCTGGGTGACCCATGGGAGATCCGAATGGAGGCCCTCGGGGG GGCATGCCCATTGGAGGAGGTCCAGGATGAGGCATTCCAGGTGGTGGTCGGGGCGGTGGC TGGCCCCCAGAGCCTGGGGGTCCAGCGTGGGGATGTCCTAAGCCATGAGGGCCATGGTGT GCAAGCTGCATCTGAGACATCCCTGGATGGGGCATCCCACCNCGTGGGAATGGGTGAGGA TGTGAGTGTCCATGACCAGGATGTCCTGCCCCTGGGGTTCCTGCCGATGGGGGGCCATGT CCTGCAGCCCCAGGAAGCATANGTGGTGGGGGCATGGCTGGGGGTATCCCCAGTGGGAGG GCTTCANNGAGTGGCACTGGNGGNTGGAAAGGAGCCAGNNNAGAGCATGCCCTGNTGGAG AAGNCCCAGACCCCCATGATGATACCCACAGNATGGGGAGCANAGGTGAAGGAAGTGCAT CTGCAAACACTGATGAGGGCGATCACCTGGGAAAGCCGGTTCTGACTGCCAAAAATCTTC GGTGCTAACCATGGCCTACCTCTGAATCTTCTA |
Restriction Sites | NotI-NotI |
ACCN | NM_005850 |
Insert Size | 1430 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_005850.3, NP_005841.1 |
RefSeq Size | 2001 bp |
RefSeq ORF | 2001 bp |
Locus ID | 10262 |
UniProt ID | Q15427 |
Domains | RRM |
Protein Pathways | Spliceosome |
Gene Summary | This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210352 | SF3B4 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 4, 49kDa (SF3B4) |
CNY 3,656.00 |
|
RC210352L3 | Lenti-ORF clone of SF3B4 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 4, 49kDa (SF3B4) |
CNY 5,890.00 |
|
RC210352L4 | Lenti-ORF clone of SF3B4 (mGFP-tagged)-Human splicing factor 3b, subunit 4, 49kDa (SF3B4) |
CNY 5,890.00 |
|
RG210352 | SF3B4 (tGFP-tagged) - Human splicing factor 3b, subunit 4, 49kDa (SF3B4) |
CNY 4,370.00 |