MANBA (NM_005908) Human Untagged Clone
CAT#: SC116441
MANBA (untagged)-Human mannosidase, beta A, lysosomal (MANBA)
CNY 6,448.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | MANB1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116441 sequence for NM_005908 edited (data generated by NextGen Sequencing)
ATGCGCCTCCACCTGCTCCTGCTGCTCGCGCTGTGCGGTGCAGGCACCACCGCCGCGGAG CTCAGTTACAGCTTGCGTGGCAACTGGAGCATCTGCAATGGGAACGGCTCGCTGGAGCTG CCCGGGGCGGTCCCTGGCTGCGTGCACAGCGCCTTGTTCCAGCAGGGCCTGATCCAGGAT TCTTACTACAGATTTAATGACCTTAACTACAGATGGGTCTCTTTGGATAACTGGACCTAT AGCAAAGAATTTAAAATCCCCTTTGAAATTAGCAAATGGCAAAAAGTAAATTTGATTCTT GAGGGAGTGGATACGGTTTCAAAAATCCTGTTCAATGAAGTCACTATTGGGGAAACAGAC AATATGTTCAATAGATATAGCTTTGATATTACCAACGTGGTCAGGGACGTGAACTCCATT GAGCTGCGTTTCCAGTCAGCGGTGTTGTATGCAGCACAGCAGAGCAAAGCTCACACTCGC TACCAGGTTCCCCCAGACTGCCCTCCACTTGTGCAGAAGGGTGAATGCCATGTCAACTTT GTTCGGAAGGAGCAATGTTCCTTTAGTTGGGACTGGGGGCCTTCCTTTCCTACCCAGGGA ATCTGGAAAGATGTTAGAATTGAAGCCTATAATATTTGTCACCTGAACTACTTCACATTT TCCCCAATATATGATAAGAGTGCCCAGGAGTGGAATCTGGAAATAGAGTCTACATTTGAT GTTGTCAGCTCAAAGCCAGTTGGTGGTCAAGTGATCATAGCCATCCCTAAGTTGCAAACA CAACAGACATACAGCATTGAACTTCAACCTGGGAAAAGGATTGTTGAGCTATTTGTGAAC ATTAGCAAGAATATTACTGTAGAAACTTGGTGGCCTCATGGACATGGAAACCAGACTGGG TACAACATGACTGTTCTTTTTGAACTGGATGGAGGCTTAAATATTGAAAAATCAGCTAAG GTTTATTTTAGGACAGTGGAACTTATAGAAGAGCCTATAAAAGGGTCTCCTGGTTTGAGT TTCTATTTCAAAATTAATGGATTTCCCATATTTCTAAAAGGCTCAAACTGGATCCCAGCA GATTCATTCCAGGACCGAGTAACCTCTGAGTTGTTACGGCTCCTTTTACAGTCTGTTGTG GATGCTAATATGAATACTCTTCGGGTTTGGGGAGGAGGAATTTATGAGCAGGATGAATTC TATGAACTCTGTGATGAACTAGGAATAATGGTATGGCAGGATTTTATGTTTGCCTGTGCC CTTTATCCAACTGATCAGGGCTTCCTGGATTCAGTGACAGCAGAAGTTGCCTACCAGATC AAGAGACTGAAATCTCATCCTTCTATCATCATATGGAGTGGCAATAATGAAAATGAGGAG GCGCTGATGATGAATTGGTATCATATCAGTTTCACTGACCGGCCAATCTACATCAAGGAC TATGTGACACTCTATGTGAAAAACATCAGAGAGCTCGTACTGGCAGGAGACAAGAGTCGT CCTTTTATTACGTCCAGTCCTACAAATGGGGCTGAAACTGTTGCAGAAGCCTGGGTCTCT CAAAACCCTAATAGCAATTATTTTGGTGATGTACATTTTTATGACTATATCAGTGATTGC TGGAACTGGAAAGTTTTCCCAAAAGCTCGATTTGCATCTGAATATGGATATCAGTCCTGG CCGTCCTTCAGTACATTAGAAAAGGTCTCGTCTACAGAGGACTGGTCTTTCAATAGCAAG TTTTCACTTCATCGACAACATCACGAAGGTGGTAACAAACAAATGCTTTATCAGGCTGGA CTTCATTTCAAACTCCCCCAAAGCACAGATCCATTACGCACATTTAAAGATACCATCTAC CTTACTCAGGTGATGCAGGCCCAGTGTGTCAAAACAGAAACTGAATTCTACCGCCGTAGT CGCAGCGAGATAGTGGATCAGCAAGGGCACACGATGGGGGCACTTTATTGGCAGTTGAAT GACATCTGGCAAGCTCCTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTGGAAAATGCTT CATTACTTTGCTCAGAATTTCTTTGCTCCACTGTTGCCAGTAGGCTTTGAGAATGAAAAC ACGTTCTATATCTATGGTGTGTCAGATCTTCACTCGGATTATTCGATGATACTCAGTGTG AGAGTCCATACATGGAGCTCCCTGGAGCCCGTGTGCTCTCGTGTGACTGAACGTTTTGTG ATGAAAGGAGGAGAGGCTGTCTGCCTTTATGAGGAGCCAGTGTCTGAATTGCTGAGGAGA TGTGGGAATTGCACACGGGAAAGCTGTGTGGTTTCCTTTTACCTTTCAGCTGACCATGAA CTCCTGAGCCCGACCAACTACCACTTCTTGTCCTCACCGAAGGAGGCCGTGGGGCTCTGC AAGGCGCAGATCACTGCCATCATCTCTCAGCAAGGTGACATATTTGTTTTTGACCTGGAG ACCTCAGCTGTCGCTCCCTTTGTTTGGTTGGATGTAGGAAGCATCCCAGGGAGATTTAGT GACAATGGTTTCCTCATGACTGAGAAGACACGAACTATATTATTTTACCCTTGGGAGCCC ACCAGCAAGAATGAGTTGGAGCAATCTTTTCATGTGACCTCCTTAACAGATATTTACTGA Clone variation with respect to NM_005908.3 757 g=>a;2150 c=>t >OriGene 5' read for NM_005908 unedited
GTTAGGATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCTCCACATCT CGGTGGCGCGGGATCTCAAGAGCGCCTCCACCTGCTCCTGCTGCTCGCGCTGTGCGGTGC AGGCACCACCGCCGCGGAGCTCAGTTACAGCTTGCGTGGCAACTGGAGCATCTGCAATGG GAACGGCTCGCTGGAGCTGCCCGGGGCGGTCCCTGGCTGCGTGCACAGCGCCTTGTTCCA GCAGGGCCTGATCCAGGATTCTTACTACAGATTTAATGACCTTAACTACAGATGGGTCTC TTTGGATAACTGGACCTATAGCAAAGAATTTAAAATCCCCTTTGAAATTAGCAAATGGCA AAAAGTAAATTTGATTCTTGAGGGAGTGGATACGGTTTCAAAAATCCTGTTCAATGAAGT CACTATTGGGGAAACAGACAATATGTTCAATAGATATAGCTTTGATATTACCAACGTGGT CAGGGACGTGAACTCCATTGAGCTGCGTTTCCAGTCAGCGGTGTTGTATGCAGCACAGCA GAGCAAAGCTCACACTCGCTACCAGGTTCCCCCAGACTGCCCTCCACTTGTGCAGAAGGG TGAATGCCATGTCAACTTTGTTCGGAAGGAGCAATGTTCCTTTAGTTGGGACTGGGGGCC TTCCTTTCCTACCCAGGGAATCTGGAAAGATGTTAGAATTGAAGCCTATAATATTTGTCA CCTGAACTACTTCACATTNTCCCCATATATGATAAGAGTGCCCAGGAGTGGAATCTGGAA ATAGAGTCTACATTCTGATGTGTCAGCTCAAGCCAGTTGGTGGTCAAGTGATCATAGCCA TNCCCTAGTTGCAAACACACAGACATACAGCATTGAACTTCAACCTGGAAAAAGGATGTT GAGCCTATTGTGAACACTAGCAGAATATT >OriGene 3' read for NM_005908 unedited
ATGGACCGAGGCTCCCCTTCTACAACGAGTTTTTCTTTTTTTTTTTTTTTTTTTACAAGG TATTTTATATAACTTTTTTATTTTTATATACACTTTCACAGTGGACAGAAAAAATACCCA TATACCTTTACCCAAATAGCTACCACCAAAAAATCTCTGTGGAAACATTTCAATCGCCAT TCCCTCTGAAATAATAACAAAGCACTTTATTTGAGTATTCCGTATTCCCCAAAGCTAGCT GGGAAAGACCTACATCACCTCAAAACCTTCCATTGGGTTCCATAGATCCTGCCATGTCAC ATTCTTGTAACCAGCTGCAGGGCCATCTTGTTATAACTGGTTCATGTCCTGCTAAAGCTG AAAGGGGAAGGGCTAAAAACACAGTCCTGGCACAGATTCCAGGACACCCCGGCACAGGCT TGTTTGAGGACATTGCCTGGGTAAACAGCCTCCCCTGAAAGTGTTCAGGGTACAACTCTT CACAGAGCAATCGTTCAAATGCGGGGCAGCACGCAGACATGTCTCTCGGCTTCTCTCCTT GACTCTGTGGCCTTCCTCTCCAGTCGGTGCTTTAAAAATGCTTTATTCCCATGGTCCACT GAAAATACAACCTAAATTCCTTCAGTAAATATCTGTCAAGGAGGCCACATGAAAAGATTG CTCCAACTCATTTCTGCTGGATGGCCTCCAAGGGTAAAAAATATAATTCGTGTCTTCTCA GCCATGAAGGAACCCTTGCCCCTACATCTCCCTGGGATGCTTCCTACTTCAACCAAACAA AGTAGCGACCGTTGAAGTCTCCAGGTCAAAAAAAATAGTCACCTTGCTGAAATAGATGCA CTGATCGGCGCTTGAAAACCCCAGGTCTCCTCCGGGAGGACACAAGGGAATTGGCGGCCT CAGATTTATGTCACCTGAAGGAAAGGAACCCCCATTTTCCGGGGAATCCAACTTCTAAAA TAAAACATCCTCCTAAAGGGAAAGCTTC |
Restriction Sites | NotI-NotI |
ACCN | NM_005908 |
Insert Size | 3160 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_005908.2, NP_005899.2 |
RefSeq Size | 3308 bp |
RefSeq ORF | 2640 bp |
Locus ID | 4126 |
UniProt ID | O00462 |
Domains | Glyco_hydro_2_C |
Protein Pathways | Lysosome, Other glycan degradation |
Gene Summary | This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204862 | MANBA (Myc-DDK-tagged)-Human mannosidase, beta A, lysosomal (MANBA) |
CNY 6,440.00 |
|
RC204862L3 | Lenti ORF clone of Human mannosidase, beta A, lysosomal (MANBA), Myc-DDK-tagged |
CNY 8,840.00 |
|
RC204862L4 | Lenti ORF clone of Human mannosidase, beta A, lysosomal (MANBA), mGFP tagged |
CNY 9,030.00 |
|
RG204862 | MANBA (tGFP-tagged) - Human mannosidase, beta A, lysosomal (MANBA) |
CNY 8,040.00 |