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PRMT5 (NM_006109) Human Untagged Clone
CAT＃: SC116333
PRMT5 (untagged)-Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1
CNY 6,208.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HRMT1L5; HSL7; IBP72; JBP1; SKB1; SKB1Hs |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006109, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTCCAGCGGGAGGGACCTGAATTGCGTCC CCGAAATAGCTGACACACTAGGGGCTGTGGCCAAGCAGGGGTTTGATTTCCTCTGCATGCCTGTCTTCCA TCCGCGTTTCAAGAGGGAGTTCATTCAGGAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGAC CTACTGCTGTCAGGAAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACT CAAAAGTGGAGAAGATTCGCAGGAACTCCGAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGCATATTT GGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGCCAGAGTTTTGACCAAC CACATCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGTACCCTTGGTGGCACCAGAGGACCTGA GAGATGATATAATTGAGAATGCACCAACTACACACACAGAGGAGTACAGTGGGGAGGAGAAAACGTGGAT GTGGTGGCACAACTTCCGGACTTTGTGTGACTATAGTAAGAGGATTGCAGTGGCTCTTGAAATTGGGGCT GACCTCCCATCTAATCATGTCATTGATCGCTGGCTTGGGGAGCCCATCAAAGCAGCCATTCTCCCCACTA GCATTTTCCTGACCAATAAGAAGGGATTTCCTGTTCTTTCTAAGATGCACCAGAGGCTCATCTTCCGGCT CCTCAAGTTGGAGGTGCAGTTCATCATCACAGGCACCAACCACCACTCAGAGAAGGAGTTCTGCTCCTAC CTCCAATACCTGGAATACTTAAGCCAGAACCGTCCTCCACCTAATGCCTATGAACTCTTTGCCAAGGGCT ATGAAGACTATCTGCAGTCCCCGCTTCAGCCACTGATGGACAATCTGGAATCTCAGACATATGAAGTGTT TGAAAAGGACCCCATCAAATACTCTCAGTACCAGCAGGCCATCTATAAATGTCTGCTAGACCGAGTACCA GAAGAGGAGAAGGATACCAATGTCCAGGTACTGATGGTGCTGGGAGCAGGACGGGGACCCCTGGTGAACG CTTCCCTGCGGGCAGCCAAGCAGGCCGACCGGCGGATAAAGCTGTATGCTGTGGAGAAAAACCCAAATGC CGTGGTGACGCTAGAGAACTGGCAGTTTGAAGAATGGGGAAGCCAAGTGACCGTAGTCTCATCAGACATG AGGGAATGGGTGGCTCCAGAGAAAGCAGACATCATTGTCAGTGAGCTTCTGGGCTCATTTGCTGACAATG AATTGTCGCCTGAGTGCCTGGATGGAGCCCAGCACTTCCTAAAAGATGATGGTGTGAGCATCCCCGGGGA GTACACTTCCTTTCTGGCTCCCATCTCTTCCTCCAAGCTGTACAATGAGGTCCGAGCCTGTAGGGAGAAG GACCGTGACCCTGAGGCCCAGTTTGAGATGCCTTATGTGGTACGGCTGCACAACTTCCACCAGCTCTCTG CACCCCAGCCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATGATTGACAACAACCGCTATTGCAC CTTGGAATTTCCTGTGGAGGTGAACACAGTACTACATGGCTTTGCCGGCTACTTTGAGACTGTGCTTTAT CAGGACATCACTCTGAGTATCCGTCCAGAGACTCACTCTCCTGGGATGTTCTCATGGTTTCCCATCCTCT TCCCTATTAAGCAGCCCATAACGGTACGTGAAGGCCAAACCATCTGTGTGCGTTTCTGGCGATGCAGCAA TTCCAAGAAGGTGTGGTATGAGTGGGCTGTGACAGCACCAGTCTGTTCTGCTATTCATAACCCCACAGGC CGCTCATATACCATTGGCCTCTAG >OriGene 5' read for NM_006109 unedited
GTTCANAATTGTATACGACNACTATAGGCGGCNCGCGAATCGGCACCAGGCCTCGTGCCG AATTCGGCACGAGGAGATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTC CAGCGGGAGGGACCTGAATTGCGTCCCCGAAATAGCTGACACACTAGGGGCTGTGGCCAA GCAGGGGTTTGATATCCTCTGCATGCCTGTCTTCCATCCGCGTTTCAAGAGGGAGTTCAT TCAGGAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGACCTACTGCTGTCAGG AAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACTCAAA AGTGGAGAAGATTCGCAGGAACTCCGAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGC ATATTTGGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGC CAGAGTTTTGACCAACCACATCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGT ACCCTTGGTGGCACCAGAGGACCTGAGAGATGATATAATTGAGAATGCACCAACTACACA CACAGAGGAGTACAGTGGGGAGGAGAAAACGTGGATGTGGTGGCACAACTTCCGGACTTT GTGTGACTATAGTAAGAGGATTGCAGTGGCTCTTGAAAATGGGCTGACCTCCCATCTAAT CATGTCATTGATCGCTGGCTTGGGGAGCCCATCANAGCAGCCATTCTCCCCACTAGCATT TTCCTGACCAAATAGAAGGNNATTCCTGNTNNCTTCTAGATGCACCAGAGGCTCATCTTT CCGCTCCTCAGNTGGAGGTGCAGTTCATCATCACAGGCACAACCACACTCAGANAAGGAG GTCTGCTCCTACCTCCATA >OriGene 3' read for NM_006109 unedited
CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTGGCGGGCAGTAAAGGGCTATAACTTTATTTGTATTTCCTCTTACCCAAAACCCTTAA AACAAGAACCGAAAAAGGGTGAAAATCCGTTCAAACCCCATGTTCTCAGGGATATTCCAG GGAGTTCTTGAGGGTGAGTGCGTAACTTTAAATCCAGCACTAATTCCTCACCCCCTGGCC TGAGGGCTTCATAAATTGGGGGCTTGAACCCTGCAATTAATTATAATCCCTTGCCCACCT TGATGTAAGGCAGGAAAGCAAATTGAAATGCTCCTCTCTGATGGGCAAGGGGAATCACAG CCCATAAATGTACTGCACCTTTTGTACTACAGGAACAAAACCTGAAGCTGCTTTCAAGGG TCTGGACACTTGGCACGCAGGGCTAAAGGCCAATGGTATATGAACGGCCTGTGGGGTTAT GAATAACAAAACAGACTGGTGCTTGTACAACCCACTCATACCACACCTTTTTGGAAATGC TGGCTCGCCAAAAACCCCCACAGATGGGTTGGGCCTTACGTACCCGTATTGGCTGCTTAA TAAGGAAGAAGATGGGAAACCATTAAAACCTTCCAGGAAAGTGAGTTTCTTGACGGCTAC CTCCGATGGATGCCTCGATAAACCCAGTCCCAAGGTACCCCGGAAAAGCCTGTGTATCTG GGTTACCTCCACAGAAATTCCAAGGCAAAACGGGGTGTCGCCATATAGGAACCTTGTGGG TAGGCTGAAGGAAAACACGGCTGGGGTCAAAAACTTGTGGAAATTCGCACCCCACACCTT AAGGGTCTTAAAAGGGCCCTAGGTACGGGGCTTCTCTACAGGTGGACTTATTGTAACTTG GGGAAAACGGACCCCAAGAATGTCTCCCGGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006109 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006109.2, NP_006100.2 |
| RefSeq Size | 2541 bp |
| RefSeq ORF | 2541 bp |
| Locus ID | 10419 |
| UniProt ID | O14744 |
| Domains | Skb1 |
| Protein Families | Stem cell - Pluripotency |
| Gene Summary | This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) encodes the longest isoform (a). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PRMT5- mediated symmetric arginine dimethylation is attenuated by mutant huntingtin and is impaired in Huntington's Disease (HD)
,Ratovitski, T;Arbez, N;Stewart, JC;Chighladze, E;Ross, CA;,
Cell Cycle
,PubMed ID 25927346
[PRMT5]
|
|
Proliferative role of TRAF4 in breast cancer by upregulating PRMT5 nuclear expression
,Yang, F;Wang, J;Ren, HY;Jin, J;Wang, AL;Sun, LL;Diao, KX;Wang, EH;Mi, XY;,
Tumour Biol.
,PubMed ID 25704480
[PRMT5]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203458 | PRMT5 (Myc-DDK-tagged)-Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1 |
CNY 4,744.00 |
|
| RC203458L1 | Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, Myc-DDK-tagged |
CNY 7,144.00 |
|
| RC203458L2 | Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC203458L3 | Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, Myc-DDK-tagged |
CNY 7,144.00 |
|
| RC203458L4 | Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mGFP tagged |
CNY 7,144.00 |
|
| RG203458 | PRMT5 (tGFP-tagged) - Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1 |
CNY 6,344.00 |
