PCBP1 (NM_006196) Human Untagged Clone

CAT#: SC116302

PCBP1 (untagged)-Human poly(rC) binding protein 1 (PCBP1)



  "NM_006196" in other vectors (6)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
PCBP1 mouse monoclonal antibody, clone OTI3D12 (formerly 3D12)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HEL-S-85; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116302 sequence for NM_006196 edited (data generated by NextGen Sequencing)
ATGGATGCCGGTGTGACTGAAAGTGGACTAAATGTGACTCTCACCATTCGGCTTCTTATG
CACGGAAAGGAAGTAGGAAGCATCATTGGGAAGAAAGGGGAGTCGGTTAAGAGGATCCGC
GAGGAGAGTGGCGCGCGGATCAACATCTCGGAGGGGAATTGTCCGGAGAGAATCATCACT
CTGACCGGCCCCACCAATGCCATCTTTAAGGCTTTCGCTATGATCATCGACAAGCTGGAG
GAAGATATCAACAGCTCCATGACCAACAGTACCGCGGCCAGCAGGCCCCCGGTCACCCTG
AGGCTGGTGGTGCCGGCCACCCAGTGCGGCTCCCTGATTGGGAAAGGCGGGTGTAAGATC
AAAGAGATCCGCGAGAGTACGGGGGCGCAGGTCCAGGTGGCGGGGGATATGCTGCCCAAC
TCCACCGAGCGGGCCATCACCATCGCTGGCGTGCCGCAGTCTGTCACCGAGTGTGTCAAG
CAGATTTGCCTGGTCATGCTGGAGACGCTCTCCCAGTCTCCGCAAGGGAGAGTCATGACC
ATTCCGTACCAGCCCATGCCGGCCAGCTCCCCAGTCATCTGCGCGGGCGGCCAAGATCGG
TGCAGCGACGCTGCGGGCTACCCCCATGCCACCCATGACCTGGAGGGACCACCTCTAGAT
GCCTACTCGATTCAAGGACAACACACCATTTCTCCGCTCGATCTGGCCAAGCTGAACCAG
GTGGCAAGACAACAGTCTCACTTTGCCATGATGCACGGCGGGACCGGATTCGCCGGAATT
GACTCCAGCTCTCCAGAGGTGAAAGGCTATTGGGCAAGTTTGGATGCATCTACTCAAACC
ACCCATGAACTCACCATTCCAAATAACTTAATTGGCTGCATAATCGGGCGCCAAGGCGCC
AACATTAATGAGATCCGCCAGATGTCCGGGGCCCAGATCAAAATTGCCAACCCAGTGGAA
GGCTCCTCTGGTAGGCAGGTTACTATCACTGGCTCTGCTGCCAGTATTAGTCTGGCCCAG
TATCTAATCAATGCCAGGCTTTCCTCTGAGAAGGGCATGGGGTGCAGCTAG

Clone variation with respect to NM_006196.3
>OriGene 5' read for NM_006196 unedited
TCACTATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCGCT
CGCCATGGATGCCGGTGTGACTGAAAGTGGACTAAATGTGACTCTCACCATTCGGCTTCT
TATGCACGGAAAGGAAGTAGGAAGCATCATTGGGAAGAAAGGGGAGTCGGTTAAGAGGAT
CCGCGAGGAGAGTGGCGCGCGGATCAACATCTCGGAGGGGAATTGTCCGGAGAGAATCAT
CACTCTGACCGGCCCCACCAATGCCATCTTTAAGGCTTTCGCTATGATCATCGACAAGCT
GGAGGAAGATATCAACAGCTCCATGACCAACAGTACCGCGGCCAGCAGGCCCCCGGTCAC
CCTGAGGCTGGTGGTGCCGGCCACCCAGTGCGGCTCCCTGATTGGGAAAGGCGGGTGTAA
GATCAAAGAGATCCGCGAGAGTACGGGGGCGCAGGTCCAGGTGGCGGGGGATATGCTGCC
CAACTCCACCGAGCGGGCCATCACCATCGCTGGCGTGCCGCAGTCTGTCACCGAGTGTGT
CAAGCAGATTTGCCTGGTCATGCTGGAGACGCTCTCCCAGTCTCCGCAAGGGAGAGTCAT
GACCATTCCGTACCAGCCCATGCCGGCCAGCTCCCCAGTCATCTGCGCGGGCGGCCAAGA
TCGGTGCAGCGACGCTGCGGGCTACCCCCATGCCACCCATGACCTGNAGGGACCACCTCT
AGATGCCTACTCGATTCAGGGACACACACCATTTCTCCGCTCGATCTGGCCAAGCTGAAC
CCAGTGGCAAGAAAACAGTCTCACTTTGCCATGATGCCACGCGGNACCGGATTCGCCGNA
ATTTGACTCCACTCTCCACAAGTGAAAGGCTATTGCGGCCAGTTGGATGCCTCTACTCAA
ACACNCATGAACCTCCCATTTCCAATAATCAATTGCTT
>OriGene 3' read for NM_006196 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACCAATCCGTTTTTTAATTCTTA
AATTCTTGACATTACAGAACTAAACTGAAATTTATTAACATTCCACTCTTACATTTCTTA
TCGACAAACAGAAATAAAATTCATGAGCCAAAAAACCCAAAACAAAACTAAAAACAGGGA
AAAGCTTATAAAACTAAATATGGATCCCAGCATTAACAGCTGAACAGAGAATGTGATTTT
TAAATTCTTAGCGGATGATAAAGTTGTGTAGAAACTGAACACTTACAAATTATTTAAAAC
CTGGAATCACTGACCAGAAATTACACAGTTGGATCATGGGAGAACAGCAGAAAGGGGTTA
TTGAGGGAACCTACACTGTTCTAGCTGCACCCCATGCCCTTCTCAGAGGAAAGCCTGGCA
TTGATTAGATACTGGGCCAGACTAATACTGGCAGCAGAGCCAGTGATAGTAACCTGCCTA
CCAGAGGAGCCTTCCACTGGGTTGGCAATTTTGATCTGGGCCCCGGACATCTGGCGGATC
TCATTAATGTTGGCGCCTTGGCGCCCGATTATGCAGCCAATTAAGTTATTTGGAATGGTG
AGTTCATGGGTGGTTTGAGTAGATGCATCCAAACTTGCCCAATAGCCTTTCACCTCTGGA
GAGCTGGAGTCAATTCCGGCGAATCCGGTCCCGCCGTGCATCATGGGCAAGTGAGACTGG
TGTCTTGCCACCTGGTTCAGCTTGGCCAGATCGAGCGGAGAAAGGTGGGGTNGTCCTTGA
ATCGAGTAGGCATCTAGAGGGTGGTCCCTNNCAGTCATGGGTGGGCATGGGGGTANCCCG
CANCGTCNCTGCACCGATCTTGGCCGCCGCGCAAATACTGGGGAACTGGNCCGCATGGGC
TGGACNGAAATGGCATGACTTTCCCTTGCGAGACTGGGGAAAGCGTTCCACATGACAGGC
AATTTGCTGACAACTTGTGACGATGGGGACGCACGAG
Restriction Sites NotI-NotI     
ACCN NM_006196
Insert Size 1470 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006196.2, NP_006187.1
RefSeq Size 1634 bp
RefSeq ORF 1071 bp
Locus ID 5093
UniProt ID Q15365
Domains KH
Protein Pathways Spliceosome
Gene Summary This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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