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PCBP1 (NM_006196) Human Untagged Clone
CAT＃: SC116302
PCBP1 (untagged)-Human poly(rC) binding protein 1 (PCBP1)
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-S-85; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116302 sequence for NM_006196 edited (data generated by NextGen Sequencing)
ATGGATGCCGGTGTGACTGAAAGTGGACTAAATGTGACTCTCACCATTCGGCTTCTTATG CACGGAAAGGAAGTAGGAAGCATCATTGGGAAGAAAGGGGAGTCGGTTAAGAGGATCCGC GAGGAGAGTGGCGCGCGGATCAACATCTCGGAGGGGAATTGTCCGGAGAGAATCATCACT CTGACCGGCCCCACCAATGCCATCTTTAAGGCTTTCGCTATGATCATCGACAAGCTGGAG GAAGATATCAACAGCTCCATGACCAACAGTACCGCGGCCAGCAGGCCCCCGGTCACCCTG AGGCTGGTGGTGCCGGCCACCCAGTGCGGCTCCCTGATTGGGAAAGGCGGGTGTAAGATC AAAGAGATCCGCGAGAGTACGGGGGCGCAGGTCCAGGTGGCGGGGGATATGCTGCCCAAC TCCACCGAGCGGGCCATCACCATCGCTGGCGTGCCGCAGTCTGTCACCGAGTGTGTCAAG CAGATTTGCCTGGTCATGCTGGAGACGCTCTCCCAGTCTCCGCAAGGGAGAGTCATGACC ATTCCGTACCAGCCCATGCCGGCCAGCTCCCCAGTCATCTGCGCGGGCGGCCAAGATCGG TGCAGCGACGCTGCGGGCTACCCCCATGCCACCCATGACCTGGAGGGACCACCTCTAGAT GCCTACTCGATTCAAGGACAACACACCATTTCTCCGCTCGATCTGGCCAAGCTGAACCAG GTGGCAAGACAACAGTCTCACTTTGCCATGATGCACGGCGGGACCGGATTCGCCGGAATT GACTCCAGCTCTCCAGAGGTGAAAGGCTATTGGGCAAGTTTGGATGCATCTACTCAAACC ACCCATGAACTCACCATTCCAAATAACTTAATTGGCTGCATAATCGGGCGCCAAGGCGCC AACATTAATGAGATCCGCCAGATGTCCGGGGCCCAGATCAAAATTGCCAACCCAGTGGAA GGCTCCTCTGGTAGGCAGGTTACTATCACTGGCTCTGCTGCCAGTATTAGTCTGGCCCAG TATCTAATCAATGCCAGGCTTTCCTCTGAGAAGGGCATGGGGTGCAGCTAG Clone variation with respect to NM_006196.3 >OriGene 5' read for NM_006196 unedited
TCACTATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCGCT CGCCATGGATGCCGGTGTGACTGAAAGTGGACTAAATGTGACTCTCACCATTCGGCTTCT TATGCACGGAAAGGAAGTAGGAAGCATCATTGGGAAGAAAGGGGAGTCGGTTAAGAGGAT CCGCGAGGAGAGTGGCGCGCGGATCAACATCTCGGAGGGGAATTGTCCGGAGAGAATCAT CACTCTGACCGGCCCCACCAATGCCATCTTTAAGGCTTTCGCTATGATCATCGACAAGCT GGAGGAAGATATCAACAGCTCCATGACCAACAGTACCGCGGCCAGCAGGCCCCCGGTCAC CCTGAGGCTGGTGGTGCCGGCCACCCAGTGCGGCTCCCTGATTGGGAAAGGCGGGTGTAA GATCAAAGAGATCCGCGAGAGTACGGGGGCGCAGGTCCAGGTGGCGGGGGATATGCTGCC CAACTCCACCGAGCGGGCCATCACCATCGCTGGCGTGCCGCAGTCTGTCACCGAGTGTGT CAAGCAGATTTGCCTGGTCATGCTGGAGACGCTCTCCCAGTCTCCGCAAGGGAGAGTCAT GACCATTCCGTACCAGCCCATGCCGGCCAGCTCCCCAGTCATCTGCGCGGGCGGCCAAGA TCGGTGCAGCGACGCTGCGGGCTACCCCCATGCCACCCATGACCTGNAGGGACCACCTCT AGATGCCTACTCGATTCAGGGACACACACCATTTCTCCGCTCGATCTGGCCAAGCTGAAC CCAGTGGCAAGAAAACAGTCTCACTTTGCCATGATGCCACGCGGNACCGGATTCGCCGNA ATTTGACTCCACTCTCCACAAGTGAAAGGCTATTGCGGCCAGTTGGATGCCTCTACTCAA ACACNCATGAACCTCCCATTTCCAATAATCAATTGCTT >OriGene 3' read for NM_006196 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACCAATCCGTTTTTTAATTCTTA AATTCTTGACATTACAGAACTAAACTGAAATTTATTAACATTCCACTCTTACATTTCTTA TCGACAAACAGAAATAAAATTCATGAGCCAAAAAACCCAAAACAAAACTAAAAACAGGGA AAAGCTTATAAAACTAAATATGGATCCCAGCATTAACAGCTGAACAGAGAATGTGATTTT TAAATTCTTAGCGGATGATAAAGTTGTGTAGAAACTGAACACTTACAAATTATTTAAAAC CTGGAATCACTGACCAGAAATTACACAGTTGGATCATGGGAGAACAGCAGAAAGGGGTTA TTGAGGGAACCTACACTGTTCTAGCTGCACCCCATGCCCTTCTCAGAGGAAAGCCTGGCA TTGATTAGATACTGGGCCAGACTAATACTGGCAGCAGAGCCAGTGATAGTAACCTGCCTA CCAGAGGAGCCTTCCACTGGGTTGGCAATTTTGATCTGGGCCCCGGACATCTGGCGGATC TCATTAATGTTGGCGCCTTGGCGCCCGATTATGCAGCCAATTAAGTTATTTGGAATGGTG AGTTCATGGGTGGTTTGAGTAGATGCATCCAAACTTGCCCAATAGCCTTTCACCTCTGGA GAGCTGGAGTCAATTCCGGCGAATCCGGTCCCGCCGTGCATCATGGGCAAGTGAGACTGG TGTCTTGCCACCTGGTTCAGCTTGGCCAGATCGAGCGGAGAAAGGTGGGGTNGTCCTTGA ATCGAGTAGGCATCTAGAGGGTGGTCCCTNNCAGTCATGGGTGGGCATGGGGGTANCCCG CANCGTCNCTGCACCGATCTTGGCCGCCGCGCAAATACTGGGGAACTGGNCCGCATGGGC TGGACNGAAATGGCATGACTTTCCCTTGCGAGACTGGGGAAAGCGTTCCACATGACAGGC AATTTGCTGACAACTTGTGACGATGGGGACGCACGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006196 |
| Insert Size | 1470 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006196.2, NP_006187.1 |
| RefSeq Size | 1634 bp |
| RefSeq ORF | 1071 bp |
| Locus ID | 5093 |
| UniProt ID | Q15365 |
| Domains | KH |
| Protein Pathways | Spliceosome |
| Gene Summary | This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207878 | PCBP1 (Myc-DDK-tagged)-Human poly(rC) binding protein 1 (PCBP1) |
CNY 3,656.00 |
|
| RC207878L1 | Lenti ORF clone of Human poly(rC) binding protein 1 (PCBP1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207878L2 | Lenti ORF clone of Human poly(rC) binding protein 1 (PCBP1), mGFP tagged |
CNY 5,890.00 |
|
| RC207878L3 | Lenti ORF clone of Human poly(rC) binding protein 1 (PCBP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207878L4 | Lenti ORF clone of Human poly(rC) binding protein 1 (PCBP1), mGFP tagged |
CNY 5,890.00 |
|
| RG207878 | PCBP1 (tGFP-tagged) - Human poly(rC) binding protein 1 (PCBP1) |
CNY 5,256.00 |
