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NFYB (NM_006166) Human Untagged Clone
CAT＃: SC116285
NFYB (untagged)-Human nuclear transcription factor Y, beta (NFYB)
CNY 2,400.00
CNY 2,950.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CBF-A; CBF-B; HAP3; NF-YB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_006166 edited
GAATTCGGCACGAGGCCGCGGCGGAGGGAGGGGAGGCCGAGTCCTGGAAGCGGAGCTCCG CGCTGGGACTGGTTCCTTCGCAGCCATTTTCTGTCCAACCAAACAGCCGATTGGAGACGG GAGCCAACCAGGGCTGCATTGGAGGTTGAAATCACAAAGATTAGACACCTTTTTAGATAG GTGTTCTTCAGCACCACTGACAACACGGTTCTGACAGTATTTCATGACAATGGATGGTGA CAGTTCTACAACAGATGCTTCTCAACTAGGAATCTCTGCAGACTATATTGGAGGAAGTCA TTATGTTATACAGCCTCATGATGATACTGAGGACAGCATGAATGATCATGAAGACACAAA TGGTTCAAAAGAAAGTTTCAGAGAACAAGATATATATCTTCCAATAGCAAACGTGGCTAG GATAATGAAAAATGCCATACCTCAAACGGGAAAGATTGCAAAAGATGCCAAAGAATGTGT TCAAGAATGTGTAAGTGAGTTCATCAGTTTTATAACATCTGAAGCAAGTGAAAGGTGCCA TCAAGAGAAACGGAAAACAATCAATGGAGAAGATATTCTCTTTGCTATGTCTACTTTAGG CTTTGACAGTTATGTGGAACCTCTGAAATTATACCTTCAGAAATTCAGAGAGGCTATGAA AGGAGAAAAGGGAATTGGTGGAGCAGTCACAGCTACAGATGGACTAAGTGAAGAGCTTAC AGAGGAGGCATTTACTAACCAGTTACCAGCTGGCTTAATAACCACAGACGGTCAACAACA AAATGTTATGGTTTACACAACATCATATCAACAGATTTCTGGTGTTCAGCAAATTCAGTT TTCATGATCTGAAGAAATGATGGAATGGGGAGTGTAGAGAAATGAGAGTCTGTATGATTC TGGAACAGAGACATCAGAAGGAAAGACTGGTGAAAAGATGTATCTTTGTATATTAATAGC TGTAATGTAGCTTCCTGATGCTTGACTAATTGAGGTGTTAATTCTGACTTGAGAATCTTT TTCATGAATGATTTTAAAGAAAAATTTGGATTTTAAAGGTATTAAAATATTTTTGTTTTG TACGAGAGTTTGTTGCTCTGTATGACTCCTGTATGCATTGTATATTGCAATTTATTACTG TCAGAGATTTGTAGACAGTTTCTTATTTTCATATTGAATCATGTTACTTTTGTAATTCAA GTAAGCGGCTGGGTTAATTCATGATGTTTGCCCTTTTAATAAAATATAAGGGTAGAGTTC ATTTTGAATGCAAGTTGCCTTTATTATAAATTTGAGTTTGTCTTGGTTATACCTTGCATG ATAACCTAGCTAGATTTCTAGCATTTGCTGTATTTATTAAAATTATTATTTTTTTGGTAA AACATTAATAGTTTAAGCAGCATCATTTTTTTAAAAAATGTAATTGAATAAGTGTGAATG CAGAAGCAAATATTGTCTGCCCTGTTAAACTTGGTGCCCATTAACAGTGTTTACACTGTT CATCGTGCCTGTTAATGTAGTTTTAGTTACTGGAGCTTTTTTAAGACTAGATTTGGTTTT GAGTTACATTTTTAAGAATGTGGGAATATATTTAAGTTTAATGTAGTCCTAGTGCTCTTG AAATGGTGCCCCTTTCATTTGGTACATGATTTTTTTTCAAATCATATCTTCAAGTACTAT AGTATTCTCTTACAGAAGAGGAGTTTTATAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XGTCTTCATTTTACCTTTTTAATTGAAATGTCAAGTTTCCTGTTACACTATGGAAACCAA GAAACATCAGACATCATTGCGTGTACAGACCTTTTGCATGGGTGAGTGGATGAAATGGAG AACAGAGTGAGTGCTGTGAACGGTGTGAAATAGAAGCCAACTTCTAGTATGCTGTCTTCA TCTCTGCAATAAACTAAACGTAAATAATGTAACTTTGTATAATTTAGTAGTATGTACTTG GATTGTTTCTTTTTGAAAAGTGAGGATTTGTATTTTCTCATCTATTCTTTTTGGTGTTAA TTAACTTGAGGGATTTCTTTTTTAAGTTTTTAATATCAAAAATTGCTATTGTATAGTTTG CCTAAAAGGAAGTTGGAGTGAATATCATCTTTGAAGGACACTCAAATTTATAAGGTAGAA GCAATTTCCCAGATATAGGCTGAACAGAAACATTTACTTTGCCAGAAGGAGCCTTTAGGA GTATATGTGGGCTAATCAGAGGTTAATAATGTCCTCATCTCCTACTCTCCATTTAAAAAA CAAACTTCATTGTAGTAAATTCAAATAGGAAAAAATGGGGGAGTAGTTAAGCAAAATCAA CAGTCATCCTACTATGTTGAAAGAACCAATGTTAATATTTTGATAAATATCATTTGTCCT TTTACTTTGCTAAATATGTATATTTTTATAAAAATGGGCTCATATTGTGCATACCATTTT GTAACCTGCTTTTTTCACTTAACAATATATTGGGAACATCTTTAAAAAAAAAAAAAAAAA AAAACTCGAC >OriGene 5' read for NM_006166 unedited
AGGTCGAATTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGGCCGCGGCGG AGGGAGGGGAGGCCGAGTCCTGGAAGCGGAGCTCCGCGCTGGGACTGGTTCCTTCGCAGC CATTTTCTGTCCAACCAAACAGCCGATTGGAGACGGGAGCCAACCAGGGCTGCATTGGAG GTTGAAATCACAAAGATTAGACACCTTTTTAGATAGGTGTTCTTCAGCACCACTGACAAC ACGGTTCTGACAGTATTTCATGACAATGGATGGTGACAGTTCTACAACAGATGCTTCTCA ACTAGGAATCTCTGCAGACTATATTGGAGGAAGTCATTATGTTATACAGCCTCATGATGA TACTGAGGACAGCATGAATGATCATGAAGACACAAATGGTTCAAAAGAAAGTTTCAGAGA ACAAGATATATATCTTCCAATAGCAAACGTGGCTAGGATAATGAAAAATGCCATACCTCA AACGGGAAAGATTGCAAAAGATGCCAAAGAATGTGTTCAAGAATGTGTAAGTGAGTTCAT CAGTTTTATAACATCTGAAGCAAGTGAAAGGTGCCATCAAGAGAAACGGAAAACAATCAA TGGAGAAGATATTCTCTTTGCTATGTCTACTTTAGGCTTTGACAGTTATGTGGAACCTCT GAAATTATACCTTCAGAAATTCAGAGAGGCTATGAAAGGAGAAAAGGGAATTGGGGGAGC AGTCACAGCTACAGATGGACTAAGTGAAGAGCTTACAGAGGAGGCATTTACTAACCAGTT ACCAGCTGGCTTAATAACCACAGACGGTCACAACAAATGTTAATGGTTTACACAACATCA TATAACAGATTTTTTNTGTTCACCANATTCAGTTTCATGATCTGAACAATGATGAAAGGG GGGAGTGT >OriGene 3' read for NM_006166 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTAAAAATGTTCCCAA TATATTGTTAAGTGAAAAAAGCGGTTACAAAATGGTATGCACAATATGAGCCCATTTTTA TAAAAATATACATATTTAGCAAAGTAAAAGGACAAATGATATTTATCAAAATATTAACAT TGGTTCTTTCAACATAGTAGGATGACTGTTGATTTTGCTTAACTACTCCCCCATTTTTTC CTATTTGAATTTACTACAATGAAGTTTGTTTTTTAAATGGAGAGTAGGAGATGAGGACAT TATTAACCTCTGATTAGCCCACATATACTCCTAAAGGCTCCTTCTGGCAAAGTAAATGTT TCTGTTCAGCCTATATCTGGGAAATTGCTTCTACCTTATAAATTTGAGTGTCCTTCAAAG ATGATATTCACTCCAACTTCCTTTTAGGCAAACTATACAATAGCAATTTTTGATATTAAA AACTTAAAAAAGAAATCCCTCAAGTTAATTAACACCAAAAAGAATAGATGAGAAAATACA AATCCTCACTTTTCAAAAAGAAACAATCCAAGTACATACTACTAAATTATACAAAGTTAC ATTATTTACGTTTAGTTTATTGCAGAGATGAAGACAGCATACTAGAAGTTGGCTTCTATT TCACACCGTTCACAGCACTCACTCTGTTCTCCATTTCATCCACTCACCCATGCAAAAGGT CTGTACACCCAATGATGCCTGATGGTTCTTGCGTTCCATAGCGCAACAAGAAAACTCGAC ATTTCAATTATAAAGGGTAAATGAAGACCTTAACCATCANACTATTAAACTGCTCTTNTG TAAGAGGATCCTATATTACTTGAAAATTTAATTGAAAAAAAATCATGTCCCAAATGAAGG GGCCCCTTTCAGAGCCTAGGACTACCTTAACTTAATTATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006166 |
| Insert Size | 2850 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006166.3, NP_006157.1 |
| RefSeq Size | 3482 bp |
| RefSeq ORF | 624 bp |
| Locus ID | 4801 |
| UniProt ID | P25208 |
| Domains | CBFD_NFYB_HMF |
| Protein Families | Transcription Factors |
| Protein Pathways | Antigen processing and presentation |
| Gene Summary | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Regulation of the p53 transcriptional response by structurally diverse core promoters
,null,
Genes & Development
,PubMed ID 20040571
[NFYB]
|
|
Regulation of the p53 transcriptional response by structurally diverse core promoters
,José M. Morachis, Christopher M. Murawsky, and Beverly M. Emerson,
Genes & Dev., Jan 2010; 24: 135 - 147
[NFYB]
|
Documents
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Resources
Other Versions
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| RC202749L2 | Lenti ORF clone of Human nuclear transcription factor Y, beta (NFYB), mGFP tagged |
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| RC202749L3 | Lenti ORF clone of Human nuclear transcription factor Y, beta (NFYB), Myc-DDK-tagged |
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| RC202749L4 | Lenti ORF clone of Human nuclear transcription factor Y, beta (NFYB), mGFP tagged |
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| RG202749 | NFYB (tGFP-tagged) - Human nuclear transcription factor Y, beta (NFYB) |
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