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Hsp40 (DNAJB1) (NM_006145) Human Untagged Clone
CAT＃: SC116273
DNAJB1 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1)
CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Hdj1; Hsp40; HSPF1; RSPH16B; Sis1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116273 sequence for NM_006145 edited (data generated by NextGen Sequencing)
ATGGGTAAAGACTACTACCAGACGTTGGGCCTGGCCCGCGGCGCGTCGGACGAGGAGATC AAGCGGGCCTACCGCCGCCAGGCGCTGCGCTACCACCCGGACAAGAACAAGGAGCCCGGC GCCGAGGAGAAGTTCAAGGAGATCGCTGAGGCCTACGACGTGCTCAGCGACCCGCGCAAG CGCGAGATCTTCGACCGCTACGGGGAGGAAGGCCTAAAGGGGAGTGGCCCCAGTGGCGGT AGCGGCGGTGGTGCCAATGGTACCTCTTTCAGCTACACATTCCATGGAGACCCTCATGCC ATGTTTGCTGAGTTCTTCGGTGGCAGAAATCCCTTTGACACCTTTTTTGGGCAGCGGAAC GGGGAGGAAGGCATGGACATTGATGACCCATTCTCTGGCTTCCCTATGGGCATGGGTGGC TTCACCAACGTGAACTTTGGCCGCTCCCGCTCTGCCCAAGAGCCCGCCCGAAAGAAGCAA GATCCCCCAGTCACCCACGACCTTCGAGTCTCCCTTGAAGAGATCTACAGCGGCTGTACC AAGAAGATGAAAATCTCCCACAAGCGGCTAAACCCCGACGGAAAGAGCATTCGAAACGAA GACAAAATATTGACCATCGAAGTGAAGAAGGGGTGGAAAGAAGGAACCAAAATCACTTTC CCCAAGGAAGGAGACCAGACCTCCAACAACATTCCAGCTGATATCGTCTTTGTTTTAAAG GACAAGCCCCACAATATCTTTAAGAGAGATGGCTCTGATGTCATTTATCCTGCCAGGATC AGCCTCCGGGAGGCTCTGTGTGGCTGCACAGTGAACGTCCCCACTCTGGACGGCAGGACG ATACCCGTCGTATTCAAAGATGTTATCAGGCCTGGCATGCGGCGAAAAGTTCCTGGAGAA GGCCTCCCCCTCCCCAAAACACCCGAGAAACGTGGGGACCTCATTATTGAGTTTGAAGTG ATCTTCCCCGAAAGGATTCCCCAGACATCAAGAACCGTACTTGAGCAGGTTCTTCCAATA TAG Clone variation with respect to NM_006145.1 >OriGene 5' read for NM_006145 unedited
AGACAGAATTGTATACGACTTACTATAGGCGGCCGCGAATTCGCACGAGGTAGAGTCCGG GACTGGTCGGCGGCGGAGCCGGGGGACGGCGACAGCGGGTCGGCGGGCCGCAGGAGGGGG TCATGGGTAAAGACTACTACCAGACGTTGGGCCTGGCCCGCGGCGCGTCGGACGAGGAGA TCAAGCGGGCCTACCGCCGCCAGGCGCTGCGCTACCACCCGGACAAGAACAAGGAGCCCG GCGCCGAGGAGAAGTTCAAGGAGATCGCTGAGGCCTACGACGTGCTCAGCGACCCGCGCA AGCGCGAGATCTTCGACCGCTACGGGGAGGAAGGCCTAAAGGGGAGTGGCCCCAGTGGCG GTAGCGGCGGTGGTGCCAATGGTACCTCTTTCAGCTACACATTCCATGGAGACCCTCATG CCATGTTTGCTGAGTTCTTCGGTGGCAGAAATCCCTTTGACACCTTTTTTGGGCAGCGGA ACGGGGAGGAAGGCATGGACATTGATGACCCATTCTCTGGCTTCCCTATGGGCATGGGTG GCTTCACCAACGTGAACTTTGGCCGCTCCCGCTCTGCCCAAGAGCCCGCCCGAAAGAAGC AAGATCCCCCAGTCACCCACGACCTTCGAGTCTCCCTTGAAGAGATCTACAGCGGCTGTA CCAAGAAGATGAAAATCTCCCACAAGCGGCTAAACCCCGACGGAAAGAGCATTCGAAACG AAGACAAAATATTGACCATCGAAGTGAAGAAGGGGTGGAAAGAANGAACCAAAATCACTT TCCCCAAGGAAGGAGACCAGACCTCCAACAACATTCCAGCTGATATCGTCTTTGTTTTAA AGGACAAGCCCCACATATCTTTAGAGAGATGGCTCTGATGTCTTTATCCTGCCAGGATCA GCCTCCGGGAGCTC >OriGene 3' read for NM_006145 unedited
GAGTCGAGTTTTTTTTTTTTTTTTTTGAGGTTTAGCATCAGTCTTTAATGCTGTCGCACT TCATTGACTAGTCACACACTTTGGTCTCATGTTGGCAGTGGCAACTTACAATGAGTCTAA AGGTCTGAGCACTGGACTGGCCTCCAGGGAACAGACATTTGTTCCAACTCCCCTTCCCTC CCCAAGATTTCTTCAGAATTCCATTATGGCCCTATACAGAGAGCACTGGCTGCCGGGGAC AGGTTTTGAGTGTACACCAACTATACATGGAATTATAAAAACATATTTACAGACGTTCCA CAGTGCTCCTGTAATCAGAAGCAAAGACCCTTTTATCAAAAGGGATTGTATCTAGGGCTG TGCAAAATTCAAAAGGATCAGATCCCTTTGAAAGAGTCCATAGTCCATGAAACAAAAAAT ACCCTGGCCACTGGTAAGCCCCACGTGTGCCAAGATTGCCTTACAGAAATGTAAAGAGTG TGACCCACAAAGTGAGGACATTCAGCTTTACTGGAGCCAGAGGTCAGGAAAGGCCCCTTG CTGAGGCCACGCTCCCAGACCATCAAGGGAAGAGGTGGTTGCACTTGCTGGGATCACTCA CTTGAAGCGTGCTCCCTTGGTCCTGCTGGAACAACAGGGATTGTTGGTGACCCCACCCCG CAGGTCTTCCGGAATCTTTAAGGTTTACACTTGGCACGGCACAAAGTAAAAACACCTTCC CAGAGATCAACCATACTTGGCGAACAATTCCGAACAACGCCCATCCTCGTCCCCCCCTTT ACAAGGAGGGGGGGGCAATGGGGTCTGGCAATCCCCCTCGCCCGCGTGCCTTTTTTATCT TCCCTATATCCAACGACACCCCGCGCTCATGCCCCCCGCGGGCCGCCCATCCTACATCAT GGCAGGTACTTGGTGTGGGTTTGTGTAACTTTCGGGAGGCGCGTAACACCATGGGGGCCA AAGCGATTGCCCTTTCGGCTAGCCATCCACGTTAGCATTAAATTATCACAACCCCCGTTA TCTGCGCCCACCAACAAGGCATTCGCCGTTCAATACATTTCCCCCACCCCTCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006145 |
| Insert Size | 2290 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006145.1, NP_006136.1 |
| RefSeq Size | 2233 bp |
| RefSeq ORF | 1023 bp |
| Locus ID | 3337 |
| UniProt ID | P25685 |
| Domains | DnaJ, DnaJ_C |
| Gene Summary | This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Tracking Mutant Huntingtin Aggregation Kinetics in Cells Reveals Three Major Populations That Include an Invariant Oligomer Pool
,Maya A. Olshina, Lauren M. Angley, Yasmin M. Ramdzan, Jinwei Tang, Michael F. Bailey, Andrew F. Hill, and Danny M. Hatters,
J. Biol. Chem., Jul 2010; 285: 21807 - 21816
[DNAJB1]
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| RC201762L2 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1), mGFP tagged |
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| RC201762L3 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1), Myc-DDK-tagged |
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| RC201762L4 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1), mGFP tagged |
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| RG201762 | DNAJB1 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1) |
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