ACADM (NM_000016) Human Untagged Clone
CAT#: SC116219
ACADM (untagged)-Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ACAD1; MCAD; MCADH |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116219 sequence for NM_000016 edited (data generated by NextGen Sequencing)
ATGGCAGCGGGGTTCGGGCGATGCTGCAGGGTCCTGAGAAGTATTTCTCGTTTTCATTGG AGATCACAGCATACAAAAGCCAATCGACAACGTGAACCAGGATTAGGATTTAGTTTTGAG TTCACCGAACAGCAGAAAGAATTTCAAGCTACTGCTCGTAAATTTGCCAGAGAGGAAATC ATCCCAGTGGCTGCAGAATATGATAAAACTGGTGAATATCCAGTCCCCCTAATTAGAAGA GCCTGGGAACTTGGTTTAATGAACACACACATTCCAGAGAACTGTGGAGGTCTTGGACTT GGAACTTTTGATGCTTGTTTAATTAGTGAAGAATTGGCTTATGGATGTACAGGGGTTCAG ACTGCTATTGAAGGAAATTCTTTGGGGCAAATGCCTATTATTATTGCTGGAAATGATCAA CAAAAGAAGAAGTATTTGGGGAGAATGACTGAGGAGCCATTGATGTGTGCTTATTGTGTA ACAGAACCTGGAGCAGGCTCTGATGTAGCTGGTATAAAGACCAAAGCAGAAAAGAAAGGA GATGAGTATATTATTAATGGTCAGAAGATGTGGATAACCAACGGAGGAAAAGCTAATTGG TATTTTTTATTGGCACGTTCTGATCCAGATCCTAAAGCTCCTGCTAATAAAGCCTTTACT GGATTCATTGTGGAAGCAGATACCCCAGGAATTCAGATTGGGAGAAAGGAATTAAACATG GGCCAGCGATGTTCAGATACTAGAGGAATTGTCTTCGAAGATGTGAAAGTGCCTAAAGAA AATGTTTTAATTGGTGACGGAGCTGGTTTCAAAGTTGCAATGGGAGCTTTTGATAAAACC AGACCTGTAGTAGCTGCTGGTGCTGTTGGATTAGCACAAAGAGCTTTGGATGAAGCTACC AAGTATGCCCTGGAAAGGAAAACTTTCGGAAAGCTACTTGTAGAGCACCAAGCAATATCA TTTATGCTGGCTGAAATGGCAATGAAAGTTGAACTAGCTAGAATGAGTTACCAGAGAGCA GCTTGGGAGGTTGATTCTGGTCGTCGAAATACCTATTATGCTTCTATTGCAAAGGCATTT GCTGGAGATATTGCAAATCAGTTAGCTACTGATGCTGTGCAGATACTTGGAGGCAATGGA TTTAATACAGAATATCCTGTAGAAAAACTAATGAGGGATGCCAAAATCTATCAGATTTAT GAAGGTACTTCACAAATTCAAAGACTTATTGTAGCCCGTGAACACATTGACAAGTACAAA AATTAA Clone variation with respect to NM_000016.4 >OriGene 5' read for NM_000016 unedited
TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGTGCCACCCCGCCTAGCG CAGCGCCCCGTCCTTCCGCAGCCCAACCGCCTCTTCCCGCCCCGCCCCATCCCGCCCACG GGCTCCAGTGGGCGGGACCAGAGGAGTCCCGCGTTCGGGGAGTATGTCAAGGCCGTGACC CGTGTATTATTGTCCGAGTGGCCGGAACGGGAGCCAACATGGCAGCGGGGTTCGGGCGAT GCTGCAGGGTCCTGAGAAGTATTTCTCGTTTTCATTGGAGATCACAGCATACAAAAGCCA ATCGACAACGTGAACCAGGATTAGGATTTAGTTTTGAGTTCACCGAACAGCAGAAAGAAT TTCAAGCTACTGCTCGTAAATTTGCCAGAGAGGAAATCATCCCAGTGGCTGCAGAATATG ATAAAACTGGTGAATATCCAGTCCCCCTAATTAGAAGAGCCTGGGAACTTGGTTTAATGA ACACACACATTCCAGAGAACTGTGGAGGTCTTGGACTTGGAACTTTTGATGCTTGTTTAA TTAGTGAAGAATTGGCTTATGGATGTACAGGGGTTCAGACTGCTATTGAAGGAAATTCTT TGGGGCAAATGCCTATTATTATTGCTGGAAATGATCAACAAAAGAAGAAGTATTTGGGGA GAATGACTGANGAGCCATTGATGTGTGCTTATTGTGTAACAGAACCTGGAGCANGCTCTG ATGTAGCTGGGTATAAGACCANAGCAGAAAAGAAAGGAGATGAGTATATTATTAATGGTC AGAAGATGTGGATTACCAACGGNAGGAAAAGCTATTGGTATTTTTATTGGCACGTTCTGA TCCAGATCCTAAAGCTNCTGCTAATAAAGCCTTTACTGGATTCATTGGGGAAGCAGATAC CCANG >OriGene 3' read for NM_000016 unedited
CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAAGCACTGA ACAATTTTATTAAGTTCTTTGCCAAAATGCAAACATATGAAATGAGATTGTGTACAATAG ACCAAAGGCTACCTAGAGACCAACAGTCATATAAAAATAATTTAAGGCAAGCTTTATTGA TTTATTTTAAATAAGGTAAGTGAAATATGGGCTCAGAATTAAATAAGATTTTCAAGAAAG TTCTTGTAGCTGTAAAATAGGAGAATATGAATGTCAAAATTTTGACTGTTTCTGTGCCAA ACAGAAAGTTTCACAAATGCTTTCCAAACTGCAATAAAAAGAACTTTAATAATACCATTA AGTCTACAAAGTTACTGTCAAGTTGACAGTATTCAAATACTACAATATTTTCTAAATGTA AATTTTGTAACATCTTGAATACTTTCTAAGCCCACTTTTCTGTCATTACAAAAATAAAAT TCCAAGAAATTTCTGCTACATTAATCTTAAGAGATCAAAATAACCTATGTAGTTTTCTAG GTTAATGTAATTCAAGTAAAGTGGTACTAAAGAAAACACATCATTTTAATAAAAACCAAA CCTGCTTAAGAGACATAACAGAGTTAAGCAAAAGTATAAACTACTATAATAAGCAGTTGC TTAGATTTAATATAAGAGGATTTGTTTTCACTGGAAAAAACGTTAAAGCCCTTTCTTTTT TCTGGAGCTGAAACAGTGGCTTGTGTTCTAGTTATTCAATATTTCTACAGNAATTTTTTT TATTTTTTGCACTTGNNCAAGGCCGTCACGGGCCTACAATAGTCTTTGAATTTGTGAAGT ACCTTCATAATCTGATAGATTTGGCATCCCTCATTAGTTTTCTACAGGATATTCTGTATA AATCCATTGCCTCCAGTTTCTGCCAGGATCAGTAGCTACTGATCTGAATATCTCCANCAA GCCTTTGAATAGAGCCTATAATATTTCGACACACAATANCT |
Restriction Sites | NotI-NotI |
ACCN | NM_000016 |
Insert Size | 2190 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_000016.2, NP_000007.1 |
RefSeq Size | 2192 bp |
RefSeq ORF | 1266 bp |
Locus ID | 34 |
UniProt ID | P11310 |
Domains | Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N |
Protein Families | Druggable Genome |
Protein Pathways | beta-Alanine metabolism, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway, Propanoate metabolism, Valine, leucine and isoleucine degradation |
Gene Summary | This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202798 | ACADM (Myc-DDK-tagged)-Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 3,656.00 |
|
RC202798L1 | Lenti ORF clone of Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC202798L2 | Lenti ORF clone of Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC202798L3 | Lenti ORF clone of Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202798L4 | Lenti ORF clone of Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RG202798 | ACADM (tGFP-tagged) - Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,256.00 |