ZNF192 (ZKSCAN8) (NM_006298) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LD5-1; ZNF192; ZSCAN40
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC116205 sequence for NM_006298 edited (data generated by NextGen Sequencing) ATGGCTGAAGAATCAAGAAAGCCTTCAGCCCCATCCCCACCAGACCAGACTCCTGAAGAG GATCTTGTAATCGTCAAGGTAGAGGAGGATCATGGTTGGGACCAGGAATCTAGTCTGCAT GAAAGTAACCCTCTTGGCCAAGAAGTGTTCCGCCTGCGCTTCAGGCAGTTACGCTACCAG GAGACACTAGGACCCCGAGAAGCTCTGATCCAACTACGGGCCCTTTGCCATCAGTGGCTG AGGCCAGATTTGAACACCAAGGAACAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCTTG ACCATCCTACCTGAGGAGCTCCAGACACTGGTTAAGGAACATCAGCTAGAGAACGGAGAG GAGGTGGTGACCCTATTAGAGGATTTGGAAAGGCAGATTGATATACTAGGACGACCAGTC TCAGCTCGCGTACATGGACATAGGGTACTCTGGGAGGAGGTAGTACATTCAGCATCTGCA CCAGAGCCTCCAAATACTCAGCTCCAATCTGAGGCAACCCAACATAAATCTCCAGTGCCC CAAGAGTCACAAGAGAGAGCCATGTCTACTTCCCAGAGTCCTACTCGTTCCCAGAAAGGA AGTTCTGGAGACCAGGAAATGACAGCTACACTTCTCACAGCAGGGTTCCAGACTTTGGAG AAGATTGAAGACATGGCTGTGTCCCTTATTCGAGAGGAGTGGCTTCTTGATCCATCACAG AAGGATCTGTGTAGAGATAACAGGCCAGAAAATTTCAGAAACATGTTCTCCCTGGGTGGT GAGACCAGGAGTGAGAACAGGGAATTAGCTTCAAAACAGGTAATATCTACTGGAATCCAG CCACATGGAGAGACAGCTGCCAAATGCAACGGGGATGTTATCAGGGGTCTTGAGCATGAA GAAGCCCGAGACCTTCTGGGCAGATTAGAGAGGCAGCGGGGAAATCCCACACAAGAGAGA CGACATAAATGTGATGAATGTGGGAAAAGCTTTGCTCAGAGCTCAGGCCTTGTTCGCCAC TGGAGAATCCACACTGGGGAGAAACCCTATCAGTGTAATGTGTGTGGTAAAGCCTTCAGT TACAGGTCAGCCCTTCTTTCACATCAGGATATCCACAACAAAGTAAAACGCTATCACTGT AAGGAGTGTGGCAAAGCCTTCAGTCAGAACACAGGCCTGATTCTGCACCAGAGAATCCAC ACTGGGGAGAAGCCATATCAGTGCAATCAGTGTGGGAAGGCTTTCAGTCAGAGTGCGGGC CTTATTCTGCACCAGAGAATCCACAGTGGAGAGAGACCCTATGAATGTAATGAGTGTGGG AAAGCTTTCAGTCATAGCTCACACCTCATTGGACATCAGAGAATCCACACTGGGGAGAAG CCCTATGAGTGTGATGAGTGTGGGAAAACCTTCAGGCGGAGCTCACATCTTATTGGTCAT CAGAGGAGCCACACTGGGGAGAAACCCTACAAATGCAATGAGTGTGGGAGGGCCTTCAGT CAGAAGTCAGGCCTTATTGAACATCAGAGAATCCACACTGGAGAAAGACCCTATAAATGT AAAGAATGTGGGAAAGCTTTCAATGGGAACACTGGTCTCATTCAACACCTGAGAATTCAC ACAGGGGAGAAGCCCTACCAATGTAATGAGTGTGGGAAAGCCTTTATTCAGAGGTCAAGT CTCATTCGACATCAGAGAATCCACAGTGGTGAAAAATCTGAATCCATAAGCGTTTAG Clone variation with respect to NM_006298.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_006298 unedited GTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGTAGTGGCCGGA GGGGCCTGGTCCGAGTACAGCTTTCATCGCCTTTACTCCCCGACCTTCCTTCGAGTCTGT TTATCCGTTGCAGCCTCCCTTCCCCACGACGGGGCGCCTCTGCAACTCACAAAGTACCCT TAGAAAGAGGCCCTCAGAAGAGTCTTCTCTTAAGAAGATAAAGAAGGTAGTGGAAACGAA CTTCCTGAGCTTTTCAGGCTCTAATGGCTGAAGAATCAAGAAAGCCTTCAGCCCCATCCC CACCAGACCAGACTCCTGAAGAGGATCTTGTAATCGTCAAGGTAGAGGAGGATCATGGTT GGGACCAGGAATCTAGTCTGCATGAAAGTAACCCTCTTGGCCAAGAAGTGTTCCGCCTGC GCTTCAGGCAGTTACGCTACCAGGAGACACTAGGACCCCGAGAAGCTCTGATCCAACTAC GGGCCCTTTGCCATCAGTGGCTGAGGCCAGATTTGAACACCAAGGAACAGATCCTGGAGC TGCTGGTGCTGGAGCAGTTCTTGACCATCCTACCTGANGAGCTCCAGACACTGGTTAAGG AACATCAGCTAGAGAACGGAGNAGGAGGTGGTGACCCTATTAGAGGATTTGGAAAGGCAG ATTGATATACTAGGACGACCAGTCTCAGCTCGCGTACATGGACATAGGGTACTCTGGGAG GAGGTAGTACATTCAGCATCTGCACCAGAGCCTTCAATACTCAGCTNCCATCTGAGGGCA CCCACATAAATCTNCAGTGCCCCAAGATCACAAGAGAGACCATGTCTACTTNCCAGAGTC TACTCGTTCCAGAAAAGAAGTCTGGAGACAGAAATGACAGCTCACTCTACACAGGGTCAA CTTGAGAGATGAGACTGCTGGTCCTATCAAGGATGCTCTGACTCAGAGATT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_006298 unedited CCGCGGCCGCAATCTANAGTCGAGTNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTAACCTCAACAAGCTTTTTTTAAACCCCTACTTTTGGCTCAAAACTTTT CTAAGCACTTGGGGGGGCAAAAATACAAATGGAAAAGTTTTTTTTCCTGCCCTCAAGGGG CTTACAATTTAATTTTGGAAATAAATCCCAATTTTAAACTGTAACCCAAAGGGCTTAAAA ATAATTCCCAAGCCCCCTTTTTTTAAGTCCCTGGCCCCCAGTCCAAACTCAAAAAAAAAG GGCTGGGGCCCCCCAAAATGAGGGAGGGCCTCCCATGAGGGGATGGCAATCAGGGAAAAC TCCCTCCAAAAATTTAAAGGAAAAAAGGCTTTGAAGGGAGTGATAAACATTATCTGGGAC CCAAAACCCATTTAAGCAAAAAAAATTCCTTGAAAAAGGGCCCCAGGCAACAATTTGTAA AATATTCTCCAAACGCCCGACGAAATTTCAATCCTTGGAAAAACAGGGAATTTTCCTTCA AAAACAGAACGCGGTTCCACCCTTGGCTCTAAGGTTTCGGAAAATTTTCACCCCCCCGCC CTTTTAAATCCTTTTAATTGCCTTCACTTCTCGCTATCTAATTCTTTTTCCCACCTCCCC TCTATAGTTGTCCCCCACTCCCCCCCCTCCGATATCATTCTCTCGCCCTTTTCCACCTTA TACATCGCCCCCACCCATTCCCACCATTATTTCCCTCCTCTTTAACCTATTTCTCCAATA ATCTCCTTACATCTTCCCCTATCCCTCGCCACTCTCGTGTCCCACCCTCACCTTTTCTTC ACACACCTTTTTCACCCGCATACCCTTCCCCTCATCTTTTCTCTACCCACTTTCCTATAC CCTCTCTCCTTTCTTATTCCTCCTCCCCTTATTTTTCCCCCTTCCTTTTCTTTCTCCACT ATTCACCTGTCACCCATTACATACTCACCGTCCACTCCCCCCCTCTAATCCCCTTTCCCC GTCCTTCA
Restriction Sites	NotI-NotI
ACCN	NM_006298
Insert Size	7440 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006298.2, NP_006289.2
RefSeq Size	5362 bp
RefSeq ORF	1737 bp
Locus ID	7745
UniProt ID	Q15776
Domains	KRAB, LER, zf-C2H2
Protein Families	Transcription Factors
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an internal exon in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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