TXNRD2 (NM_006440) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Synonyms	GCCD5; SELZ; TR; TR-BETA; TR3; TRXR2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC116122 sequence for NM_006440 edited (data generated by NextGen Sequencing) ATGGCGGCAATGGCGGTGGCGCTGCGGGGATTAGGAGGGCGCTTCCGGTGGCGGACGCAG GCCGTGGCGGGCGGGGTGCGGGGCGCGGCGCGGGGCGCAGCAGCAGGTCAGCGGGACTAT GATCTCCTGGTGGTCGGCGGGGGATCTGGTGGCCTGGCTTGTGCCAAGGAGGCCGCTCAG CTGGGAAGGAAGGTGTCCGTGGTGGACTACGTGGAACCTTCTCCCCAAGGCACCCGGTGG GGCCTCGGCGGCACCTGCGTCAACGTGGGCTGCATCCCCAAGAAGCTGATGCACCAGGCG GCACTGCTGGGAGGCCTGATCCAAGATGCCCCCAACTATGGCTGGGAGGTGGCCCAGCCC GTGCCGCATGACTGGAGGAAGATGGCAGAAGCTGTTCAAAATCACGTGAAATCCTTGAAC TGGGGCCACCGTGTCCAGCTTCAGGACAGAAAAGTCAAGTACTTTAACATCAAAGCCAGC TTTGTTGACGAGCACACGGTTTGCGGCGTTGCCAAAGGTGGGAAAGAGATTCTGCTGTCA GCCGATCACATCATCATTGCTACTGGAGGGCGGCCGAGATACCCCACGCACATCGAAGGT GCCTTGGAATATGGAATCACAAGTGATGACATCTTCTGGCTGAAGGAATCCCCTGGAAAA ACGTTGGTGGTCGGGGCCAGCTATGTGGCCCTGGAGTGTGCTGGCTTCCTCACCGGGATT GGGCTGGACACCACCATCATGATGCGCAGCATCCCCCTCCGCGGCTTCGACCAGCAAATG TCCTCCATGGTCATAGAGCACATGGCATCTCATGGCACCCGGTTCCTGAGGGGCTGTGCC CCCTCGCGGGTCAGGAGGCTCCCTGATGGCCAGCTGCAGGTCACCTGGGAGGACAGCACC ACCGGCAAGGAGGACACGGGCACCTTTGACACCGTCCTGTGGGCCATAGGTCGAGTCCCA GACACCAGAAGTCTGAATTTGGAGAAGGCTGGGGTAGATACTAGCCCCGACACTCAGAAG ATCCTGGTGGACTCCCGGGAAGCCACCTCTGTGCCCCACATCTACGCCATTGGTGACGTG GTGGAGGGGCGGCCTGAGCTGACACCCACAGCGATCATGGCCGGGAGGCTCCTGGTGCAG CGGCTCTTCGGCGGGTCCTCAGATCTGATGGACTACGACAATGTTCCCACGACCGTCTTC ACCCCGCTGGAGTATGGCTGTGTGGGGCTGTCCGAGGAGGAGGCAGTGGCTCGCCACGGG CAGGAGCATGTTGAGGTCTATCACGCCCATTATAAACCACTGGAGTTCACGGTGGCTGGA CGAGATGCATCCCAGTGTTATGTAAAGATGGTGTGCCTGAGGGAGCCCCCACAGCTGGTG CTGGGCCTGCATTTCCTTGGCCCCAACGCAGGCGAAGTTACTCAAGGATTTGCTCTGGGG ATCAAGTGTGGGGCTTCCTATGCGCAGGTGATGCGGACCGTGGGTATCCATCCCACATGC TCTGAGGAGGTAGTCAAGCTGCGCATCTCCAAGCGCTCAGGCCTGGACCCCACGGTGACA GGCTGCTGAGGGTAA Clone variation with respect to NM_006440.3 177 c=>t;196 g=>t;1109 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_006440 unedited CCGGGCCCCCCCNNCCCNCCCCCCCCTCCCCCCCCCCCNCCCCCGTTCAAATTTGTNATA CGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCAGCCCTAGCTGCCCCAGAAGCCCC ACGACGATGGCGGCAATGGCGGTGGCGCTGCGGGGATTAGGAGGGCGCTTCCGGTGGCGG ACGCAGGCCGTGGCGGGCGGGGTGCGGGGCGCGGCGCGGGGCGCAGCAGCAGGTCAGCGG GACTATGATCTCCTGGTGGTCGGCGGGGGATCTGGTGGCCTGGCTTGTGCCAAGGAGGCC GCTCAGCTGGGAAGGAAGGTGTCCGTGGTGGACTACGTGGAACCTTCTCCCCAAGGCACC CGGTGGGGCCTCGGCGGCACCTGCGTCAACGTGGGCTGCATCCCCAAGAAGCTGATGCAC CAGGCGGCACTGCTGGGAGGCCTGATCCAAGATGCCCCCAACTATGGCTGGGAGGTGGCC CAGCCCGTGCCGCATGACTGGAGGAAGATGGCAGAAGCTGTTCAAAATCACGTGAAATCC TTGAACTGNGGCCACCGTGTCCAGCTTCAGGACAGAAAAGTCAAGTACTTTAACATCAAA GCCAGCTTTGTTGACGAGCACACGGTTTGCGGCGTTGCCAAAGGTGGGAAAGAGATTCTG CTGTCAGCCGATCACATCATCATTGCTACTGGNAGGCGGCCGAGATACCCCACGCACATC GAAGGTGCCTTGGAATATGGAAATCACAGTGATGACATCTTCTGGCTGAAGGAAATCCCT GGNAANACGTNGNTGGGTCGGGCCAGCTATGTGGNCCTGGAGTGTGCTGGCTTTCTCACC GGGATTGGGGCTGGACACACATCATGATGCGCAGCATCCCCCTTCGCGGNTTTCGACAGC AAAGGTNCTCCATGGTCATAGAACACATGGCATCTCATGGCACCCGGNTCCTGAGGGGNC TGGGGCCCCCTCCCGGGTCAGGAAGCCTTCCTGATGGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_006440 unedited TTTAAAACGGTCCTACTATGNACCGCGCCGCAATCTAAATCGAGTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTCACACTTACAAAAAAAGTACCCTCTTTATTTGCATTGCAAAAATGC CAGGGGCGCTCGGACATGGGTGCCCACAGGGTAGGTTTCCACCCAGGTCGTCATCATCTG GCACCAGGAGCCTGGGGGGGGGTGCCCGGCGGCAAAGGCTGAAAGGTGCAGGGGTGAGGT GGACAGTCCCCTGAGCCATCCCTGTGGGGCACACGGGCCACCTGTCCAGCACTTGCGCTC CACGCTGACCATCTCACAGGCGCTCCAGGAGAGGGTTGAGGCCCCCCAAACCTGGCCTGC AGCCATCCTGGGTCTGGCCTCCGAGGAGCTGGCGGCGGGCGCACCGTGTGCCCTGGCCTG CAGGGATGGCGCTTACCCTCAGCAGCCTGTCACCGTGGGGTCCAGGCCTGAGCGCTTGGA GATGCGCAGCTTGACTACCTCCTCAGAGCATGTGGGATGGATACCCACGGTCCGCATCAC CTGCGCATAGGAAGCCCCACACTTGATCCCCAGAGCAAATCCTTGAGTAACTTCGCCTGC GTTGGGGCCAAGGAAATGCAGGCCCAGCACCAGCTGTGGGGGCTCCCTCAGGCACACCAT CTTTACATAACACTGGGATGCATCTCGTCCAGCCACCGTGAACTCCAGTGGTTTATAATG GGCGTGATAGACCTCAACATGCTCCTGCCCGTGGCGAGCCACTGCCCTCTTCTTCGACAG CCCCACACAGCCTACTCCACGGGGTAGAAAACGGTCGGGGGAACATTGTCGTAGTCC
Restriction Sites	NotI-NotI
ACCN	NM_006440
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation	This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006440.2, NP_006431.2
RefSeq Size	2180 bp
Locus ID	10587
UniProt ID	Q9NNW7
Domains	pyr_redox, pyr_redox_dim
Protein Families	Druggable Genome
Protein Pathways	Pyrimidine metabolism
Gene Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene. [provided by RefSeq, Jun 2017] Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (1, also known as hTR3a). This isoform is localized to the mitochondria (PMID:16774913). CCDS Note: This CCDS ID represents the protein described in PMIDs: 9923614 and 10215850. This transcript is supported by AF106697.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 9923614. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein.

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