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Fibulin 1 (FBLN1) (NM_006486) Human Untagged Clone
CAT＃: SC116062
FBLN1 (untagged)-Human fibulin 1 (FBLN1), transcript variant D
CNY 5,160.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FBLN; FIBL1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116062 sequence for NM_006486 edited (data generated by NextGen Sequencing)
ATGGAGCGCGCCGCGCCGTCGCGCCGGGTCCCGCTTCCGCTGCTGCTGCTCGGCGGCCTT GCGCTGCTGGCGGCCGGAGTGGACGCGGATGTCCTCCTGGAGGCCTGCTGTGCGGACGGA CACCGGATGGCCACTCATCAGAAGGACTGCTCGCTGCCATATGCTACGGAATCCAAAGAA TGCAGGATGGTGCAGGAGCAGTGCTGCCACAGCCAGCTGGAGGAGCTGCACTGTGCCACG GGCATCAGCCTGGCCAACGAGCAGGACCGCTGTGCCACGCCCCACGGTGACAACGCCAGC CTGGAGGCCACATTTGTGAAGAGGTGCTGCCATTGCTGTCTGCTGGGGAGGGCGGCCCAG GCCCAGGGCCAGAGCTGCGAGTACAGCCTCATGGTTGGCTACCAGTGTGGACAGGTCTTC CGGGCATGCTGTGTCAAGAGCCAGGAGACCGGAGATTTGGATGTCGGGGGCCTCCAAGAA ACGGATAAGATCATTGAGGTTGAGGAGGAACAAGAGGACCCATATCTGAATGACCGCTGC CGAGGAGGCGGGCCCTGCAAGCAGCAGTGCCGAGACACGGGTGACGAGGTGGTCTGCTCC TGCTTCGTGGGCTACCAGCTGCTGTCTGATGGTGTCTCCTGTGAAGATGTCAATGAATGC ATCACGGGCAGCCACAGCTGCCGGCTTGGAGAATCCTGCATCAACACAGTGGGCTCTTTC CGCTGCCAGCGGGACAGCAGCTGCGGGACTGGCTATGAGCTCACAGAGGACAATAGCTGC AAAGATATTGACGAGTGTGAGAGTGGTATTCATAACTGCCTCCCCGATTTTATCTGTCAG AATACTCTGGGATCCTTCCGCTGCCGACCCAAGCTACAGTGCAAGAGTGGCTTTATACAA GATGCTCTAGGCAACTGTATTGATATCAATGAGTGTTTGAGTATCAGTGCCCCGTGCCCT ATYGGGCATACATGCATCAACACAGAGGGCTCCTACACGTGCCAGAAGAACGTGCCCAAC TGTGGCCGTGGCTACCATCTCAACGAGGAGGGAACGCGCTGTGTTGATGTGGACGAGTGC GCGCCACCTGCTGAGCCCTGTGGGAAGGGACATCGCTGCGTGAACTCTCCCGGCAGTTTC CGCTGCGAATGCAAGACGGGTTACTATTTTGACGGCATCAGCAGGATGTGTGTCGATGTC AACGAGTGCCAGCGCTACCCCGGGCGCCTGTGTGGCCACAAGTGCGAGAACACGCTGGGC TCCTACCTCTGCAGCTGTTCCGTGGGCTTCCGGCTCTCTGTGGATGGCAGGTCATGTGAA GACATCAATGAGTGCAGCAGCAGCCCCTGTAGCCAGGAGTGTGCCAACGTCTACGGCTCC TACCAGTGTTACTGCCGGCGAGGCTACCAGCTCAGCGATGTGGATGGAGTCACCTGTGAA GACATCGACGAGTGCGCCCTGCCCACCGGGGGCCACATCTGCTCCTACCGCTGCATCAAC ATCCCTGGAAGCTTCCAGTGCAGCTGCCCCTCGTCTGGCTACAGGCTGGCCCCCAATGGC CGCAACTGCCAAGACATTGATGAGTGTGTGACTGGCATCCACAACTGCTCCATCAACGAG ACCTGCTTCAACATCCAGGGCGGCTTCCGCTGCCTGGCCTTCGAGTGCCCTGAGAACTAC CGCCGCTCCGCAGCCACGCTCCAGCAGGAGAAGACAGACACGGTCCGCTGCATCAAGTCC TGCCGCCCCAACGATGTCACATGCGTGTTCGACCCCGTGCACACCATCTCCCACACCGTC ATCTCGCTGCCTACCTTCCGCGAGTTCACCCGCCCTGAAGAGATCATCTTCCTCCGGGCC ATCACGCCACCGCATCCTGCCAGCCAGGCTAACATCATCTTCGACATCACGGAAGGGAAC CTGCGGGACTCTTTTGACATCATCAAGCGTTACATGGACGGCATGACCGTGGGTGTCGTG CGCCAGGTGCGGCCCATCGTGGGCCCATTTCATGCCGTCCTGAAGCTGGAGATGAACTAT GTGGTCGGGGGCGTGGTCTCCCACCGAAATGTTGTCAACGTCCACATCTTCGTCTCTGAG TACTGGTTCTGA Clone variation with respect to NM_006486.2 963 c=>y >OriGene 5' read for NM_006486 unedited
NGGAAGCCGTTTGNGATATTTGTATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGC ACGAGGGCGGCCCTGGCCCAGCGTTGGCTGCCGAGGCTCGGCCGGAGCGTGGAGCCCGCG CCGCTGCCCCAGGACCGCGCCCGCGCCTTTGTCCGCCGCCGCCCACCGCCCGTCGCCCGC CGCCCATGGAGCGCGCCGCGCCGTCGCGCCGGGTCCCGCTTCCGCTGCTGCTGCTCGGCG GCCTTGCGCTGCTGGCGGCCGGAGTGGACGCGGATGTCCTCCTGGAGGCCTGCTGTGCGG ACGGACACCGGATGGCCACTCATCAGAAGGACTGCTCGCTGCCATATGCTACGGAATCCA AAGAATGCAGGATGGTGCAGGAGCAGTGCTGCCACAGCCAGCTGGAGGAGCTGCACTGTG CCACGGGCATCAGCCTGGCCAACGAGCAGGACCGCTGTGCCACGCCCCACGGTGACAACG CCAGCCTGGAGGCCACATTTGTGAAGAGGTGCTGCCATTGCTGTCTGCTGGGGAGGGCGG CCCAGGCCCAGGGCCAGAGCTGCGAGTACAGCCTCATGGTTGGCTACCAGTGTGGACAGG TCTTCCGGGCATGCTGTGTCAAGAGCCAGGAGACCGGAGATTTGGATGTCGGGGGCCTCC AAGAAACGGATAAGATCATTGAGGTTGAGGAGGAACAAGAGGACCCATATCTGAATGACC GCTGCCGAGGGAGCGGGCCCTGCAAGCAGCAGTGCCGAGACACGGGTGAACGAGGTGGTC TGCTCCTGCTTCGTGGGCTACCAGCTGCTGTCTGATGGTGTCTCCTGTGGAGATGTCAAT GAATGCATCACGGGCAGCCACAGCTGCCGGCTTGGGAGAATCTGCATC >OriGene 3' read for NM_006486 unedited
GCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTAAGGAGGATCACAAAGT TGTTTATTTTCCCACCTATAAAAAAAGAGGATGATGCAAAGTGCCGAAGAAATCCCAGCC ATTCCCCTGGTGAACATACTGGAGGCCCACACGAGCCTAACAGGCTGTGTCTTTACCAAG GGATTTTCCTCTATTTCAACAGAACTGCAATTTCCCTTCCAAGTGCTCCCCGAGGCATTC TGTCATGCTGCTTCTGGGGATAATTTATCATCCACGTCTGTGCTATTCTACAGCAGCCAT GTCCTTAAAACATAAAAAATCTGATTGGCCAAAAAAAAAAAAAAGTGCCAAAATGTCAAA TTTCATACAATCAGGGCAAGAACTGGTCTTCTTTTGGCATTTGGACTGGCTGCCCCCTAA AAAATGTCTTTCTTTCCTTGGCCCTTATCAAGGCCCAGCCAGAGGCAGAGAAAGGGGGTG ACACTTACCCTTCGCATTAAAAAATGAGCAAGGTTTTCAACCAAACAGTTGAGCAACTGG GCTCATTTTTTAAACACCGAAAATACATCAGATGGACTTATTCATCTGGCTCAGCTTAAT ACATGTTGGCCTAATGCTACAAATACCTAACATTAAAAAAGTCTGAGGGTATAAACAAGT ACAGACCACAGTCACTGGCAGCAATGATTGGGCCTGGAGGTGCACCTGCGGCTGTGCGAA AACCAGCCCTCAAACCAGTCCTCAGAGACCAAGATGTGGACGTTGACAACTTTTCGGAGG GAGACCACGCCCCCGACCACTATTTATTCTCCACTTCAGACAGCATGAAAGGGCCACTAT GGCCCACCTGGCCACAAACCACGTTATCCGGCCTGTAACCTTGTAAGTCAAAGAGCCCCA GCTCCCTCCGGATCAAATAAATTACCCTCCTGCAGATCCGGCCCTAGCCCCAAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006486 |
| Insert Size | 3160 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006486.2, NP_006477.2 |
| RefSeq Size | 2947 bp |
| RefSeq ORF | 2112 bp |
| Locus ID | 2192 |
| UniProt ID | P23142 |
| Domains | ANATO, EGF_CA, EGF, EGF |
| Protein Families | Secreted Protein |
| Gene Summary | Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (D) encodes the longest isoform (D). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CXCL1/GROa increases cell migration and invasion of prostate cancer by decreasing fibulin-1 expression through NF-B/HDAC1 epigenetic regulation
,Po-Lin Kuo, Kun-Hung Shen, Shun-Hsing Hung, and Ya-Ling Hsu,
Carcinogenesis, Dec 2012; 33: 2477 - 2487.
[FBLN1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221597 | FBLN1 (Myc-DDK-tagged)-Human fibulin 1 (FBLN1), transcript variant D |
CNY 5,152.00 |
|
| RC221597L3 | Lenti ORF clone of Human fibulin 1 (FBLN1), transcript variant D, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC221597L4 | Lenti ORF clone of Human fibulin 1 (FBLN1), transcript variant D, mGFP tagged |
CNY 7,700.00 |
|
| RG221597 | FBLN1 (tGFP-tagged) - Human fibulin 1 (FBLN1), transcript variant D |
CNY 6,370.00 |
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