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ME3 (NM_006680) Human Untagged Clone
CAT＃: SC115951
ME3 (untagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 4,504.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NADP-ME |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115951 sequence for NM_006680 edited (data generated by NextGen Sequencing)
ATGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGC GCCCTCCCGCGCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCG GCGCGCCCTGTGCCCCTGAAGAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAAC AAGGGGATGGCCTTTACCCTTGAAGAAAGGCTGCAGCTTGGAATCCACGGCCTAATCCCG CCCTGCTTTCTGAGCCAGGACGTCCAGCTCCTCCGAATCATGAGATATTACGAGCGGCAG CAGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAACGAGAAGCTC TTCTACCGAGTGCTGACTTCGGACGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACC GTGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATC ACCATTCATGACAAAGGTCATCTTGCAACAATGCTGAATTCTTGGCCAGAAGACAATATT AAGGCCGTGGTGGTGACTGATGGGGAGCGCATCCTGGGCCTGGGAGACCTGGGCTGCTAC GGCATGGGCATCCCTGTGGGCAAGCTGGCCCTGTACACGGCATGCGGAGGGGTGAACCCG CAGCAGTGCCTCCCTGTGCTGCTGGACGTCGGCACCAACAATGAGGAGCTGCTCAGAGAC CCTCTGTACATCGGCCTGAAACACCAGCGCGTGCACGGGAAGGCATACGATGACTTGCTG GATGAGTTCATGCAGGCTGTGACAGACAAGTTTGGAATAAATTGCCTCATCCAATTTGAA GACTTCGCCAATGCCAATGCCTTCCGCCTGCTCAACAAATACCGTAACAAGTACTGCATG TTCAATGATGACATCCAAGGCACAGCCTCCGTTGCTGTGGCAGGGATCTTGGCTGCTCTG CGAATCACCAACAACAAGCTTTCCAATCACGTGTTTGTTTTCCAAGGTGCAGGCGAGGCA GCTATGGGCATTGCCCACCTCCTTGTCATGGCCCTAGAGAAAGAAGGTGTACCGAAGGCA GAGGCCACAAGAAAGATCTGGATGGTGGACTCTAAAGGGCTCATTGTCAAGGGGAGGAGC CACCTGAACCATGAAAAGGAGATGTTTGCCCAAGACCATCCTGAAGTCAACTCCCTGGAG GAGGTGGTGAGGCTGGTGAAGCCCACAGCCATCATAGGTGTTGCTGCCATCGCAGGAGCC TTCACGGAGCAGATTCTGAGGGACATGGCCTCCTTCCACGAGCGCCCTATCATCTTTGCC CTGAGCAACCCCACCAGCAAGGCCGAGTGCACGGCTGAGAAGTGCTACCGGGTCACCGAG GGCCGAGGGATTTTTGCCAGTGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAAG ACCTTCATTCCTGGGCAGGGAAACAATGCTTACGTGTTCCCCGGGGTGGCACTGGGAGTC ATCGCCGGCGGGATCCGGCACATCCCAGATGAGATCTTCCTCCTGACAGCAGAGCAAATT GCCCAGGAAGTCTCTGAGCAGCATCTGTCCCAGGGGAGACTCTATCCACCACTCAGCACC ATCCGAGACGTGTCTTTGAGAATTGCCATCAAAGTTCTCGACTACGCGTACAAACACAAC CTGGCTTCCTACTACCCAGAGCCTAAGGACAAGGAGGCTTTTGTAAGATCCCTGGTCTAC ACTCCAGACTATGACTCCTTTACACTGGACAGCTACACTTGGCCCAAGGAAGCCATGAAT GTTCAGACGGTCTGA Clone variation with respect to NM_006680.2 972 g=>c;1605 c=>t >OriGene 5' read for NM_006680 unedited
GGGTCAATATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTCAGGCG GGCATTCTGAGCTGCTTCTTGTCACTGGGNGAAAACCACGTGCACGCAGTGCTGAGGCGC CCCACGCGCGTCCTCAGGGGAAGCCAGGCACTGAGAACAAAGGTTGTGGGACCGCTCTTT GAGGAACTCACCAGCTCCGCGAGCTCGCGGCCGCCAGCGCGGTCCACCCATTGGCGGGAG AGGACTCACAGACCGCAGCTCCTCACCCCGCCTGCTCCGCAGCGGGACCGCGTAGACCTC AGCTCCCCGAGCAGCGCAGGTGGGCCAGGGAAGGAGAGGACCGAGGTCTGCCAAGGACCA TGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGCG CCCTCCCGCGCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCGG CGCGCCCTGTGCCCCTGAAGAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAACA AGGGGATGGCCTTTACCCTTGAAGAAAGGCTGCAGCTTGGAATCCACGGCCTAATCCCGC CCTGCTTTCTGAGCCAGGACGTCCAGCTCCTCCGAATCATGAGATATTACGAGCGGCAGC AGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAACGAGAAGCTCT TCTACCGAGTGCTGACTTCGGACGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACCG TGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATCA CCATTCATGACANAGGTCATCTTTGCACAATGCTGAATCTTGGCCAGAAGACATATTAGG CA >OriGene 3' read for NM_006680 unedited
GTACGCGGCCGCTTTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTCAAAAACAAG GCTTTTATTCACTGTATGCCTTGGCATCTCCTCTCACACCAAAAAGTTTAAAAGAAAAAA CACAGCGACAAGGATTCACTTGGGCCATTTTGGAACCCATTTATATTGGGGGTGTTACTC TGGGCTTCATCTAGCCCCATACTAGCCTCTGAAACTGCCTCAAACCGTCTGAACATTCAT GGCTTCCTTGGGCCAAGTGTAGCTGTCCAGTGTAAAGGAGTCATAGTCTGGAGTGTAAAC CAGGGATCTTACAAAAGCCTCCTTGTCCTTAGGCTCTGGGTAGTAGGAAGCCAGGTTGTG TTTGTACGCGTAGTCGAAAACTTTGATGGCAATTCTCAAAGACACGTCTCGGATGGTGCT GAGTGGTGGATAAAGTCTCCCCTGGGACAGATGCTGCTCAAAGACTTCCTGGGCAATTTG CTCTGCTGTCAGGAGGAAAATCTCATCTGGGATGTGCCGGATCCCGCCGGCGATGACTCC CAGTGCCACCCCGGGGAACACGCAAGCATTGTTTCCCTGCCCAGGAATGAAGGTCTTGCC ATCTTCCAAAGCCACACTCTTAAAAGGACTTCCACTGGAAAAAATCCTTCGGTCCTTCGG AGACCCGGAAGAACTTATAAACCGCGCACTTCGGCCTTGCTGGGGGGGTTCTCCAGGGCA AAAATAATAGGCCGCTCCGGGAAGGAGGCCCTGTCCCTCAAAATCTGCTCCGTGAAAGCC TCCTGCTATGGCAGACACACCTATGTAGGGCTGTGGGCTTACACAGCCCTACCCACTTCT CCAGGGAGTCTATTTAAGAATGGCCTTGGCACAACTTTCCTTTTATGGTCCAGGGGGTTC CCCCTCACAAAGACACCTCTAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006680 |
| Insert Size | 2260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006680.2, NP_006671.2 |
| RefSeq Size | 2090 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 10873 |
| UniProt ID | Q16798 |
| Domains | malic |
| Protein Pathways | Metabolic pathways, Pyruvate metabolism |
| Gene Summary | Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) and variants 2-4 all encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224151 | ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,496.00 |
|
| RC224151L1 | Lenti-ORF clone of ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6,896.00 |
|
| RC224151L2 | Lenti-ORF clone of ME3 (mGFP-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RC224151L3 | Lenti-ORF clone of ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RC224151L4 | Lenti-ORF clone of ME3 (mGFP-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RG224151 | ME3 (tGFP-tagged) - Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6,096.00 |
