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G protein alpha 12 (GNA12) (NM_007353) Human Untagged Clone
CAT＃: SC115556
GNA12 (untagged)-Human guanine nucleotide binding protein (G protein) alpha 12 (GNA12)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | gep; NNX3; RMP |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_007353, the custom clone sequence may differ by one or more nucleotides
ATGTCCGGGGTGGTGCGGACCCTCAGCCGCTGCCTGCTGCCGGCCGAGGCCGGCGGGGCCCGCGAGCGCA GGGCGGGCAGCGGCGCGCGCGACGCGGAGCGCGAGGCCCGGAGGCGTAGCCGCGACATCGACGCGCTGCT GGCCCGCGAGCGGCGCGCGGTCCGGCGCCTGGTGAAGATCCTGCTGCTGGGCGCGGGCGAGAGCGGCAAG TCCACGTTCCTCAAGCAGATGCGCATCATCCACGGCCGCGAGTTCGACCAGAAGGCGCTGCTGGAGTTCC GCGACACCATCTTCGACAACATCCTCAAGGGCTCAAGGGTTCTTGTTGATGCACGAGATAAGCTTGGCAT TCCTTGGCAGTATTCTGAAAATGAGAAGCATGGGATGTTCCTGATGGCCTTCGAGAACAAGGCGGGGCTG CCTGTGGAGCCGGCCACCTTCCAGCTGTACGTCCCGGCCCTGAGCGCACTCTGGAGGGATTCTGGCATCA GGGAGGCTTTCAGCCGGAGAAGCGAGTTTCAGCTGGGGGAGTCGGTGAAGTACTTCCTGGACAACTTGGA CCGGATCGGCCAGCTGAATTACTTTCCTAGTAAGCAAGATATCCTGCTGGCTAGGAAAGCCACCAAGGGA ATTGTGGAGCATGACTTCGTTATTAAGAAGATCCCCTTTAAGATGGTGGATGTGGGCGGCCAGCGGTCCC AGCGCCAGAAGTGGTTCCAGTGCTTCGACGGGATCACGTCCATCCTGTTCATGGTCTCCTCCAGCGAGTA CGACCAGGTCCTCATGGAGGACAGGCGCACCAACCGGCTGGTGGAGTCCATGAACATCTTCGAGACCATC GTCAACAACAAGCTCTTCTTCAACGTCTCCATCATTCTCTTCCTCAACAAGATGGACCTCCTGGTGGAGA AGGTGAAGACCGTGAGCATCAAGAAGCACTTCCCGGACTTCAGGGGCGACCCGCACAGGCTGGAGGACGT CCAGCGCTACCTGGTCCAGTGCTTCGACAGGAAGAGACGGAACCGCAGCAAGCCACTCTTCCACCACTTC ACCACCGCCATCGACACCGAGAACGTCCGCTTCGTGTTCCATGCTGTGAAAGACACCATCCTGCAGGAGA ACCTGAAGGACATCATGCTGCAGTGA >OriGene 5' read for NM_007353 unedited
GCCAGCGAATTCGGCACGAGGCGGGGCGTGGGGCGGTGCCTCGGCCCGGGCTCGCCCTCG CCGGCGGGAGCGTCCATGGCCCCCGGGCGCCGGCGGGGCGCGGCCGCGGCCTGAGGGGCC ATGTCCGGGGTGGTGCGGACCCTCAGCCGCTGCCTGCTGCCGGCCGAGGCCGGCGGGGCC CGCGAGCGCAGGGCGGCATCGGCGCGCGCGACGCGGAGCGCGAGGCCCGGAGGCGTAGCC GCGACATCGACGCGCTGCTGGCCCGCGAGCGGCGCGCGGTCCGGCGCCTGGTGAAGATCC TGCTGCTGGGCGCGGGCGAGAGCGGCAAGTCCACGTTCCTCAAGCAGATGCGCATCATCC ACGGCCGCGAGTTCGACCAGAAGGCGCTGCTGGAGTTCCGCGACACCATCTTCGACAACA TCCTCAAGGGCTCAAGGGTTCTTGTTGATGCACGAGATAAGCTTGGCATTCCTTGGCAGT ATTCTGAAAATGAGAAGCATGGGATGTTCCTGATGGCCTTCGAGAACAAGGCGGGGCTGC CTGTGGAGCCGGCCACCTTCCAGCTGTACGTCCCGGCCCTGAGCGCACTCTGGAGGGATT CTGGCATCAGGGAGGCTTTCAGCCGGAGAAGCGAGTTTCACCTGGGGGAGTCGGTGAAGT ACTTCCTGGACAACTTGGACCGGATCGGCCAGCTGAATTACTTTTCCTATAAGCAAGATA TCCTGCTTGCTAAGA >OriGene 3' read for NM_007353 unedited
NNNNNGGGTATTGCTATGGAACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTT TTTTTTTTTTAAGCAAAGAAAGACTAATTTATTTTAATAAACATTCAGAGGCTGAGGGTG GCCAAATTTTCAAGCCAGACCCTCCCAATGTTAATACACTGTGCAAAGCTTACACCATGA ACAACTGACCCGGACCACTACCATTCCAATGGGAGCCTCTGCCTTACAATACCAGGTTAG ATGTCTGTGCCCAGAACCCCCAAATTAGTTACAACTAATAATCTTCTTTCAAGAGTTTTT TTTTTCAATCTTGGAGTTAACAATCTGACTTATTTTTCTTTTATCAAAGAAGTAGGAAAA TTACAATAAGAATTCTAGAGATCTCTATAAATTACAGACCTATGACTCAAAATCCTTGAA ACAATTTCTCTACGAACATAAGAGTTAAAAATAGATTTCAGTAAAACCCTATATGCGTCT TATGTGTACACAGTATAGCTATGAGGAACAGATCTTATTTTGAGGAACTTTATTGTTACA TTTTTGCAATAATTGAGGCACAACTACCTCCTGCTTTTCCAACATCTTAATAAAAAAGAC AATAAGGATTAACAGTGAAATTAAAATTAAAAAATACAAAAGCCTAAGGTTCTGGAGACA AAACTGACTAGAGTCTATGTGTAGCCAAGTTGTGAATGACAGTTTAGCCTTGCAGAGTTT CCTTCTTCTCCATTACAATGTGTTACAGAATTTGAAGGGGGGTGTCTTTAAAAACGTCTA TTCACCGGTCCTTGAGGAGAGAGGGGGGGAATCCCCCTTANCAGGCCAGTGCCTCGCCCG GGCGGTGGGGTCACCGCTGAACGGTCAAGACCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_007353 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007353.2, NP_031379.2 |
| RefSeq Size | 4398 bp |
| RefSeq ORF | 1146 bp |
| Locus ID | 2768 |
| UniProt ID | Q03113 |
| Domains | G-alpha |
| Protein Families | Druggable Genome |
| Protein Pathways | Long-term depression, MAPK signaling pathway, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| Gene Summary | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:22609986, PubMed:15525651, PubMed:15240885, PubMed:17565996, PubMed:12515866, PubMed:16787920, PubMed:16705036, PubMed:23762476, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866, PubMed:16202387). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (PubMed:15525651, PubMed:17565996). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production (PubMed:23762476, PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333, PubMed:16787920). Together with NAPA promotes CDH5 localization to plasma membrane (PubMed:15980433). May play a role in the control of cell migration through the TOR signaling cascade (PubMed:22609986).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210511 | GNA12 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein) alpha 12 (GNA12) |
CNY 5,488.00 |
|
| RC210511L1 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein) alpha 12 (GNA12), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210511L2 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein) alpha 12 (GNA12), mGFP tagged |
CNY 7,888.00 |
|
| RC210511L3 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein) alpha 12 (GNA12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210511L4 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein) alpha 12 (GNA12), mGFP tagged |
CNY 7,888.00 |
|
| RG210511 | GNA12 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein) alpha 12 (GNA12) |
CNY 4,370.00 |
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