OGFR (NM_007346) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC115550 sequence for NM_007346 edited (data generated by NextGen Sequencing) ATGGACGACCCCGACTGCGACTCCACCTGGGAGGAGGACGAGGAGGATGCGGAGGACGCG GAGGACGAGGACTGCGAGGACGGCGAGGCCGCCGGCGCGAGGGACGCGGACGCAGGGGAC GAGGACGAGGAGTCGGAGGAGCCGCGGGCGGCGCGGCCCAGCTCGTTCCAGTCCAGAATG ACAGGGTCCAGAAACTGGCGAGCCACGAGGGACATGTGTAGGTATCGGCACAACTATCCG GATCTGGTGGAACGAGACTGCAATGGGGACACGCCAAACCTGAGTTTCTACAGAAATGAG ATCCGCTTCCTGCCCAACGGCTGTTTCATTGAGGACATTCTTCAGAACTGGACGGACAAC TATGACCTCCTTGAGGACAATCACTCCTACATCCAGTGGCTGTTTCCTCTGCGAGAACCA GGAGTGAACTGGCATGCCAAGCCCCTCACGCTCAGGGAGGTCGAGGTGTTTAAAAGCTCC CAGGAGATCCAGGAGCGGCTTGTCCGGGCCTACGAGCTCATGCTGGGCTTCTACGGGATC CGGCTGGAGGACCGAGGCACGGGCACGGTGGGCCGAGCACAGAACTACCAGAAGCGCTTC CAGAACCTGAACTGGCGCAGCCACAACAACCTCCGCATCACACGCATCCTCAAGTCGCTG GGTGAGCTGGGCCTCGAGCACTTCCAGGCGCCGCTGGTCCGCTTCTTCCTGGAGGAGACG CTGGTGCGGCGGGAGCTGCCGGGGGTGCGGCAGAGTGCCCTGGACTACTTCATGTTCGCC GTGCGCTGCCGACACCAGCGCCGCCAGCTGGTGCACTTCGCCTGGGAGCACTTCCGGCCC CGCTGCAAGTTCGTCTGGGGGCCCCAAGACAAGCTGCGGAGGTTCAAGCCCAGCTCTCTG CCCCATCCGCTCGAGGGCTCCAGGAAGGTGGAGGAGGAAGGAAGCCCCGGGGACCCCGAC CACGAGGCCAGCACCCAGGGTCGGACCTGTGGGCCAGAGCATAGCAAGGGTGGGGGCAGG GTGGACGAGGGGCCCCAGCCACGGAGCGTGGAGCCCCAGGATGCGGGACCCCTGGAGAGG AGCCAGGGGGATGAGGCAGGGGGCCACGGGGAAGATAGGCCGGAGCCCTTAAGCCCCAAA GAGAGCAAGAAGAGGAAGCTGGAGCTGAGCCGGCGGGAGCAGCCGCCCACAGAGCCAGGC CCTCAGAGTGCCTCAGAGGTGGAGAAGATCGCTCTGAATTTGGAGGGGTGTGCCCTCAGC CAGGGCAGCCTCAGGACGGGGACCCAGGAAGTGGGCGGTCAGGACCCTGGGGAGGCAGTG CAGCCCTGCCGCCAACCCCTGGGAGCCAGGGTGGCCGACAAGGTGAGGAAGCGGAGGAAG GTGGATGAGGGTGCTGGGGACAGTGCTGCGGTGGCCAGTGGTGGTGCCCAGACCTTGGCC CTTGCCGGGTCCCCTGCCCCATCGGGGCACCCCAAGGCTGGACACAGTGAGAACGGGGTT GAGGAGGACACAGAAGGTCGAACGGGGCCCAAAGAAGGTACCCCTGGGAGCCCATCGGAG ACCCCAGGCCCCAGCCCAGCAGGACCTGCAGGGGACGAGCCAGCCGAGAGCCCATCGGAG ACCCCAGGCCCCCGCCCAGCAGGACCTGCAGGGGACGAGCCAGCCGAGAGCCCATCGGAG ACCCCAGGCCCCCGCCCGGCAGGACCTGCAGGGGACGAGCCAGCCGAGAGCCCATCGGAG ACCCCAGGCCCCAGCCCGGCAGGACCTACAAGGGATGAGCCAGCCGAGAGCCCATCGGAG ACCCCAGGCCCCCGCCCGGCAGGACCTGCAGGGGACGAGCCAGCCGAGAGCCCATCGGAG ACCCCAGGCCCCCGCCCGGCAGGACCTGCAGGGGACGAGCCAGCCGAGAGCCCATCGGAG ACCCCAGGCCCCAGCCCGGCAGGACCTACAAGGGATGAGCCAGCCAAGGCGGGGGAGGCA GCAGAGTTGCAGGACGCAGAGGTGGAGTCTTCTGCCAAGTCTGGGAAGCCTTAA Clone variation with respect to NM_007346.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_007346 unedited GCACCATTTGTNATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCCAGCGCGA GCCCCGCCGCCGCCGTACATGGACGACCCCGACTGCGACTCCACCTGGGAGACGGACGAG GAGGATGCGGAGGACGCGGAGGACGAGGACTGCGAGGACGGCGAGGCCGCCGGCGCGAGG GACGCGGACGCAGGGGACGAGGACGAGGAGTCGGAGGAGCCGCGGGCGGCGCGGCCCAGC TCGTTCCAGTCCAGAATGACAGGGTCCAGAAACTGGCGAGCCACGAGGGACATGTGTAGG TATCGGCACAACTATCCGGATCTGGTGGAACGAGACTGCAATGGGGACACGCCAAACCTG AGTTTCTACAGAAATGAGATCCGCTTCCTGCCCAACGGCTGTTTCATTGAGGACATTCTT CAGAACTGGACGGACAACTATGACCTCCTTGAGGACAATCACTCCTACATCCAGTGGCTG TTTCCTCTGCGAGAACCAGGAGTGAACTGGCATGCCAAGCCCCTCACGCTCAGGGAGGTC GAGGTGTTTAAAAGCTCCCAGGAGATCCAGGAGCGGCTTGTCCGGGCCTACGAGCTCATG CTGGGCTTCTACGGGATCCGGCTGGAGGACCGAGGCACGGGCACGGTGGGCCGAGCACAG AACTAACCAGAGCGCTTCCAGAACCTGAACTGGCGCAGCCACAACAACCTCCGCATCACA CGCATTCTCAAGTCGCTGGGTGAGCTGGGCCTCGAGCCTTTCCAGCGCCGCTGGTCCGCT TCTTCCTGTGAGAGACCCTTGTGCGGCGGGAACCTGCCCGGGGTGCCGGAAAAGTGCCCT GGACTACTTTATGTTCCCCGTGCGCTGCCGAAACCAACGCCGCCAGCTGGTGCACTTCCC CTGGGGAGCACTTCCGGCCCCCCTGCCAGTTCCCCGGGGGCCCCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_007346 unedited CCCTCAGAGGCGAGAAAAAATCCTTTTTTTTTCAAAGTCAAATGAATTTATTCAGAAAGG CCTTGCTTGGTATCAGACTAAGAAAACAGCCCTGCCCGCCGCCCCCCACTCCAGAAGGGT CAATTTACAAAGACAGGGGCGCAGGGGAGAGCTGGGTGGGGAAGACACAGCCAGGCCAGG AGGCTTCTGCAGGCCTTGTTTTTCCCTGAGGGCCTCGCGGCTTCTGGGGGCTGCTATAGG GGCCCCACAGGAGGCCAGCACTGTGGGTCATGGGTCACGGGTCACGAAGCAGAGCCTGAG GGGAGCCCGCAGCAGCTCCGGAGGCCCCAGCCCCTGCAGCAGGGACAGGAGGACCAAGAC GCCGACGGGCACTCCTTTCCTTAAGGCTTCCCAGACTTGGCAGAAGACTCCACCTCTGCG TCCTGCAACTCTGCTGCCTCCCCCGCCTTGGCTGGCTCATCCCTTGTAGGTCCTGCCGGG CTGGGGCCTGGGGTCTCCGATGGGCTCTCGGCTGGCTCGTCCCCTGCAGGTCCTGCCGGG CGGNGGCCTGGGGTCTCCGATGGGCTCTCGGCTGGCTCGTCCCCTGCAGGTCCCTGCCGG GCGGAGGCCTGGGGTCTCCGATGGGCTCTCGGCTGGCTCATCCCTTGTAGGTCCTGCCGG GCCTGGGCCTGGGGTCTCCGATGGGCTCTCGGCTGCCTCGTCCCCTGCAGGTCCTGCCGG GCGGGGCCCTGGGGTCTCCGATGGGCTCTCGGCTGGCTCGTCCCCTGCAGGTCCTGCTGG GCGGGGGCCCTGGGGTCTCCAGATGGGCTCTCGGNCTGGCTCGGTCCCTGACAGTCCTGC TGGGCTGGGGGCTGGGGTCCTCCAATGGCGTCCCAAGGGGACCCTCCTTTGGGCCCCCGT CCGAACCTCTTGTGGCCTTCCTAAACCCGGTCCTACCTGGGTCAGCCCTGGGGGCGCCCC CGANGGGGCGGNGAACCCGGCAAGGCCCAAAGTTCGCGCACCACACTGGCCACCGAGACA CGGTCCTAACACT
Restriction Sites	NotI-NotI
ACCN	NM_007346
Insert Size	2440 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007346.2, NP_031372.2
RefSeq Size	2423 bp
RefSeq ORF	2034 bp
Locus ID	11054
UniProt ID	Q9NZT2
Domains	OGFr_N, OGFr_repeat
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is a receptor for opioid growth factor (OGF), also known as [Met(5)]-enkephalin. OGF is a negative regulator of cell proliferation and tissue organization in a variety of processes. The encoded unbound receptor for OGF has been localized to the outer nuclear envelope, where it binds OGF and is translocated into the nucleus. The coding sequence of this gene contains a polymorphic region of 60 nt tandem imperfect repeat units. Several transcripts containing between zero and eight repeat units have been reported. [provided by RefSeq, Jul 2008]

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