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Claudin 12 (CLDN12) (NM_012129) Human Untagged Clone
CAT＃: SC115525
CLDN12 (untagged)-Human claudin 12 (CLDN12), transcript variant 3
CNY 2,400.00
CNY 2,950.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115525 sequence for NM_012129 edited (data generated by NextGen Sequencing)
ATGGGCTGTCGGGATGTCCACGCAGCCACAGTCCTTTCCTTCCTGTGTGGAATCGCCTCA GTAGCAGGCCTCTTTGCAGGGACTCTGCTTCCCAACTGGAGAAAATTACGATTGATCACA TTCAACAGAAACGAGAAGAACCTGACTGTTTACACAGGCCTGTGGGTGAAATGTGCCCGG TATGACGGGAGCAGTGACTGCCTGATGTACGACACTACTTGGTACTCATCAGTTGACCAG CTGGACCTGCGTGTCCTCCAGTTTGCCCTACCCCTCAGCATGCTGATCGCCATGGGTGCC CTGCTGCTCTGCCTGATTGGAATGTGCAACACTGCCTTCAGGTCCTCGGTGCCCAACATC AAACTGGCCAAGTGTCTGGTCAATAGTGCAGGTTGCCACCTGGTGGCTGGGCTGCTATTT TTCCTGGCAGGTACTGTGAGCCTCTCCCCATCTATCTGGGTCATCTTTTATAACATCCAT CTGAACAAGAAGTTTGAGCCAGTCTTTTCATTTGACTATGCAGTGTATGTCACTATTGCT AGTGCTGGGGGCCTGTTTATGACTTCCCTTATACTATTTATTTGGTATTGTACATGCAAA TCTTTGCCTTCTCCTTTCTGGCAACCATTGTACTCCCATCCACCCAGTATGCATACTTAC TCACAGCCCTATTCAGCACGCTCTCGCCTCTCTGCCATTGAAATTGACATTCCAGTAGTT TCACACACCACTTAA Clone variation with respect to NM_012129.4 >OriGene 5' read for NM_012129 unedited
TTCACATATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGCACTTCCT GGGACGCTGAGAGGGAGACGCTCCAAGAGGCTCCTCAGTGTGGGCGAGTAAAATGCCCTG CGTGTGAGAAGCAGGCTCAGATTATTGCTACTCTGTATTCAGATCTTCATGTGTCTCTTC TAAGCTGAATAAAGCTGTTTTGGAACTGTCAGGTACCTCAAAGCGTAATCTGACTGACAG TACTCCACAAGCTTGCCTGCCATGGGCTGTCGGGATGTCCACGCAGCCACAGTCCTTTCC TTCCTGTGTGGAATCGCCTCAGTAGCAGGCCTCTTTGCAGGGACTCTGCTTCCCAACTGG AGAAAATTACGATTGATCACATTCAACAGAAACGAGAAGAACCTGACTGTTTACACAGGC CTGTGGGTGAAATGTGCCCGGTATGACGGGAGCAGTGACTGCCTGATGTACGACACTACT TGGTACTCATCAGTTGACCAGCTGGACCTGCGTGTCCTCCAGTTTGCCCTACCCCTCAGC ATGCTGATCGCCATGGGTGCCCTGCTGCTCTGCCTGATTGGAATGTGCAACACTGCCTTC AGGTCCTCGGTGCCCAACATCAAACTGGCCAAGTGTCTGGTCAATAGTGCAGGTTGCCAC CTGGTGGCTGGGCTGCTATTTTTCCTGGCAGGTACTGTGAGCCTCTCCCCTCTATCTGGG TCATCTTTTATAACATCCATCTGAACAAGAAGTTTGAGCCAGTCTTTTCATTTGACTATG CAGNGTATGTCACTATTGCTAGTGCTGGGGGCCTGNTTATGACTTCCCTATACTATNTAT NTGGTATTGTACATGCCAATCTTTGCCTTCTCCTTTTCTGGCACCATTNGTACTCCATCC ACCCATATGCATACTTACTNACAGCCTATNNANNACGCTCTCGCTCTCTGCCATTGAAAT GCATNCCAGAN >OriGene 3' read for NM_012129 unedited
CTATGGACCGCGGACCGTCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTAAAAAAC ATCTTTTAATAAACATTATATAAGTACAGCGACACTTTTATTGAGGTTTTACATTGTAGT TTACAGCAAATATACTATCCATTGTAGTGTATATGTACAAGTAAAGTAAACTTATGTTTG TTTTCTCTCCCATGGCTGGATAAACCAAATCTGATACATCCAGATTTAAAGTTGTTTTCC AAGTTGGTTTACCATAAAAGGACTTTAACAATAATAGGCTTTTAATAACATAAAGGTATC CCTCCCATCACAATGAGAGCTTGACGAGGGCTCAAAAGTGACTTCAAAAACTGTAAATAA TTATTTTCCTTGGGTGGCTTTAAAAATAGCTACTGATATCAGATCAAAAACACTTAATAA AAAATACAATAAGGAAACATCTGTTAGTCTAGTGAATCCACAATAAATACCGATTTGACG GCTCTAGATTACAAAAGCCTATGATGCTTATATGAAACGTAATCGGTTATACTTACAATG CGGTGTACTAAAAAAACCGGCTGATATGTTATAAACTGAATCTCCCTTTATACAACCTTG TTTATGCTCGAGTATTACGGGGGTGGGAGAATTTTGCATCCCGCATGAATAAACCTCACG AGCTGGGACTGCACTTAATCAACACGCGACAACCCGCTTATGTGCAAATTCTACCTCGGC GGGCGCTATCAAGAATTAAGATCAGTTCCTTTGTTAAAAAACGCAAGGATCAAACACCGA CGAAATCCGACGCGCGTGCATACAAATCCAAACCTTATGTGACTTGCCGGGACAGTATAG TAAATTATTAACCTTGTTTACTCACGCCGATTAAATTTCGGTGATACTTTGTGTAAAAAT AACGAATCCGGTAACGAATACCGCAGGATACGCACAAATGCCCGGAGTCCTACACCACGG GTTACTACCCGTCCGTTCGAGCAGTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012129 |
| Insert Size | 3700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012129.2, NP_036261.1 |
| RefSeq Size | 3493 bp |
| RefSeq ORF | 735 bp |
| Locus ID | 9069 |
| UniProt ID | P56749 |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is expressed in the inner ear and bladder epithelium, and it is over-expressed in colorectal carcinomas. This protein and claudin 2 are critical for vitamin D-dependent Ca2+ absorption between enterocytes. Multiple alternatively spliced transcript variants encoding the same protein have been found.[provided by RefSeq, Sep 2011] Transcript Variant: This variant (3) lacks an exon in the 5' UTR, as compared to variant 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209820 | CLDN12 (Myc-DDK-tagged)-Human claudin 12 (CLDN12), transcript variant 3 |
CNY 2,400.00 |
|
| RC209820L1 | Lenti ORF clone of Human claudin 12 (CLDN12), transcript variant 3, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC209820L2 | Lenti ORF clone of Human claudin 12 (CLDN12), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC209820L3 | Lenti ORF clone of Human claudin 12 (CLDN12), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209820L4 | Lenti ORF clone of Human claudin 12 (CLDN12), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG209820 | CLDN12 (tGFP-tagged) - Human claudin 12 (CLDN12), transcript variant 3 |
CNY 4,370.00 |
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