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RAD54B (NM_012415) Human Untagged Clone
CAT＃: SC115426
RAD54B (untagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1
CNY 10,008.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RDH54 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115426 sequence for NM_012415 edited (data generated by NextGen Sequencing)
ATGAGACGATCTGCAGCACCAAGTCAGTTGCAGGGGAATTCCTTCAAAAAACCAAAATTT ATACCTCCAGGAAGAAGTAATCCAGGTCTGAATGAAGAGATTACAAAACTGAATCCAGAT ATAAAATTATTTGAGGGTGTTGCAATTAATAACACCTTTCTCCCGTCACAAAATGATCTT AGAATATGCAGTTTAAATCTGCCTAGTGAAGAAAGTACTAGAGAAATCAATAACAGAGAT AATTGCAGTGGAAAATATTGTTTTGAAGCACCTACACTGGCAACATTAGATCCACCTCAT ACAGTTCATTCGGCTCCTAAAGAAGTAGCAGTGTCCAAGGAACAAGAAGAGAAATCTGAT AGCCTAGTTAAATATTTCAGTGTTGTTTGGTGTAAGCCTTCAAAGAAAAAACATAAAAAG TGGGAAGGTGATGCTGTTCTTATTGTAAAAGGAAAGTCATTTATATTAAAGAATTTGGAA GGCAAAGACATTGGAAGAGGCATTGGTTATAAATTCAAAGAGCTTGAAAAGATTGAAGAG GGCCAAACACTGATGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGAT GACTTCAGCAGTGGCAGGTGTTTTCAGCTTGGAGGAGGAAGTACTGCTATCTCGCATTCT TCTCAGGTTGCCAGGAAATGTTTCTCTAACCCTTTCAAAAGTGTTTGTAAACCAAGTTCA AAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGCCATGACCCATATACGCCA AATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATAAGAACTGT TTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAG AAAGAAGGAATCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGT GGAGCTATTCTTGCTGATGAAATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATC TGGACCCTGCAGTGTCAGGGACCCTATGGAGGCAAGCCAGTAATAAAGAAGACACTAATT GTCACACCTGGAAGCTTGGTGAATAATTGGAAGAAAGAATTTCAAAAATGGCTAGGAAGT GAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTTGAAGAATTCATCAAGTCT ATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGGATCAAATT AAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCC ATTAAGACAACTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGT ACTCCAATTCAGAATGATCTGCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGA ATATTAGGCTCTTTGTCATCTTATAGGAAAATATATGAAGAACCCATCATTTTATCGAGA GAACCTTCTGCTTCTGAGGAAGAAAAGGAGTTAGGAGAAAGAAGAGCAGCTGAACTTACT TGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATTATAAATAAATATCTCCCA CCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGCTTTATCGA AAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCC CATCTAATATGTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAAC TCTATAAAGGAAAAGGAATGTAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATAC AAAGGCTTGCTAAGTGTGTTTCCTGCTGACTACAACCCTCTCCTGTTTACTGAAAAGGAG TCAGGAAAACTACAGGTGTTGTCCAAGCTCTTAGCGGTTATCCACGAACTTCGACCTACT GAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAACATTTTACAAGAAGTATGT AAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTCAAAGGCAG CAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCA AAAGCTGGTGGTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATT GATTGGAATCCAGCCACTGACATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAA TATCCTGTACATATTTACAGACTCCTAACTACAGGTACAATAGAAGAAAAGATCTATCAA AGGCAGATCAGTAAGCAAGGTCTTTGTGGGGCAGTTGTCGACCTCACCAAGACATCTGAA CATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACATTACATGAAAGTTCAGAT TGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATACAGGTGAT TCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCT AACTCCCTGAAACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGAT CATTTAAATCTTACAGATCCTTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTT CAGAATATAACCACTCAAGCTACTGGCACATAG Clone variation with respect to NM_012415.3 >OriGene 5' read for NM_012415 unedited
GCATTTGTATACGACTCACTATAGGCGGCNCGCGAATTCGCACGAGGGCTTCGTCGAGGC GGGATCCTTGAGCTTCTCCGGCGGCGAGGGGATAGCTGGTTACCAGAAGGACTTCTTTGC AGGGCCAGTGGTTTCTGTCAGATTTTCGCCGGTCACGACTGCTGAATATGAGACGATCTG CAGCACCAAGTCAGTTGCAGGGGAATTCCTTCAAAAAACCAAAATTTATACCTCCAGGAA GAAGTAATCCAGGTCTGAATGAAGAGATTACAAAACTGAATCCAGATATAAAATTATTTG AGGGTGTTGCAATTAATAACACCTTTCTCCCGTCACAAAATGATCTTAGAATATGCAGTT TAAATCTGCCTAGTGAAGAAAGTACTAGAGAAATCAATAACAGAGATAATTGCAGTGGAA AATATTGTTTTGAAGCACCTACACTGGCAACATTAGATCCACCTCATACAGTTCATTCGG CTCCTAAAGAAGTAGCAGTGTCCAAGGAACAAGAAGAGAAATCTGATAGCCTAGTTAAAT ATTTCAGTGTTGTTTGGTGTAAGCCTTCAAAGAAAAAACATAAAAAGTGGGAAGGTGATG CTGTTCTTATTGTAAAAGGAAAGTCATTTATATTAAAGAATTTGGAAGGCAAAGACATTG GAAGAGGCATTGGTTATAAATTCAAAGAGCTTGAAAAGATTGAAGAGGGCCAAACACTGA TGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGATGACTTCAGCAGTG GCAGGTGTTTTCAGCTTGGAGGGAGAAGTACTGCTATCTCGCATTCTCNCTCAGNTGCCA GAANATGTTNCTCTAAACCCTTTCAANAAGTGTTGTAAACCAAGNTCANAGGNAAATAGA CAGAATGATTTCCANAATTGCAAACCACGCCATGACCCATATACGCCCAATTCCCTCGTT ATGCCACGACCANATAAN >OriGene 3' read for NM_012415 unedited
GGGGGGNNNNGGGNGNNNNNNGNNNGGNNNNNNNNGGGGTTTTTTGAGACCTACTTCTTT TTTGCATTTAACACTTGATATTTACAAAACATTAAACCACTCAATTTTGACTATTGTATC AAAAGTGATATATTTTGCAACATATACTGTAATTTAAATAATTCTATTAATTTTCAAAAA GTACATTTAATTACCATACTAATTTTCAAAAGAAGAGCAATGGAATGTCAGAAGTAATCT TTCACTATGTGCCAGTAGCTTGAGTGGTTATATCTCTGATAAAATGAATGACACATTTTC TGTTATTCTTTCAAGAAAAGGATCTGTAAGATTTAAATGATCTCCACAAAAATGTTTCCA TTGCTTCAGCTGGGACATTAGAAAGAGGTTTCAGGGAGTTAGATTTCTGGTGATGTGGAC CAAGCTGACAATCTCTAGAGACAATGAATTTTTCCAACGAATCACCTGTATGAACTTCTT CTCCTGTACACTCACAGTCAAGCAGATCATGAGTAACACAATCTGAACTTTCATGTAATG TGAACAAATTTTTAAGTTCTTTTACTGAAAACTGAATATGTCCAGATGTCTTGGTGAGGT CTATATCTGCTCAACAAGACCTTGCTTACTGATCTGACTTTGATTCATCTTTTCCTTTTT TTGCACCCTGCTGCTAGGAGTTCCGTCAATTTGTCCAGGATATTTCCTGCCCATTCTTTC CATACCTCTACACATTGTCTGAATGGCACTGGCGCGGATTCCCATCCCTGGCCTATCAAA CATAACTCGAGATCCTCCCCACGTGGTTAATCTCCTAACACCACACTCTTTTGAACTTTA TCAAAATATTCTAAACAACAAAGAGGTTTGGCCCGGTCAAACCGCCTCACCAATTTTGCT TCCCTTTCCCAAAATCCGCTGATTTTGCCCCTCAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012415 |
| Insert Size | 3090 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012415.2, NP_036547.1 |
| RefSeq Size | 3057 bp |
| RefSeq ORF | 2733 bp |
| Locus ID | 25788 |
| UniProt ID | Q9Y620 |
| Domains | SNF2_N, DEAD, helicase_C |
| Protein Families | Druggable Genome |
| Protein Pathways | Homologous recombination |
| Gene Summary | The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201513 | RAD54B (Myc-DDK-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 9,992.00 |
|
| RC201513L1 | Lenti-ORF clone of RAD54B (Myc-DDK-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 12,392.00 |
|
| RC201513L2 | Lenti-ORF clone of RAD54B (mGFP-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 5,890.00 |
|
| RC201513L3 | Lenti-ORF clone of RAD54B (Myc-DDK-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 5,890.00 |
|
| RC201513L4 | Lenti-ORF clone of RAD54B (mGFP-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 5,890.00 |
|
| RG201513 | RAD54B (tGFP-tagged) - Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 11,592.00 |
