CMT (ICMT) (NM_012405) Human Untagged Clone

CAT#: SC115419

ICMT (untagged)-Human isoprenylcysteine carboxyl methyltransferase (ICMT)



  "NM_012405" in other vectors (4)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


ICMT rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC115419 sequence for NM_012405 edited (data generated by NextGen Sequencing)
ATGGCGGGCTGCGCGGCGCGGGCTCCGCCGGGCTCTGAGGCGCGTCTCAGCCTCGCCACC
TTCCTGCTGGGCGCCTCGGTGCTCGCGCTGCCGCTGCTCACGCGCGCCGGCCTGCAGGGC
CGCACCGGGCTGGCGCTCTACGTGGCCGGGCTCAACGCGCTGCTGCTGCTGCTCTATCGG
CCGCCTCGCTACCAGATAGCCATCCGAGCTTGTTTCCTGGGGTTTGTGTTCGGCTGCGGC
ACGCTGCTAAGTTTTAGCCAGTCTTCTTGGAGTCACTTTGGCTGGTACATGTGCTCCCTG
TCATTGTTCCACTATTCTGAATACTTGGTGACAGCAGTCAATAATCCCAAAAGTCTGTCC
TTGGATTCCTTTCTCCTGAATCACAGCCTGGAGTATACAGTAGCTGCTCTTTCTTCTTGG
TTAGAGTTCACACTTGAAAATATCTTTTGGCCAGAACTGAAGCAGATTACCTGGCTCAGT
GTCACAGGGCTGCTGATGGTGGTCTTCGGAGAATGTCTGAGGAAGGCGGCCATGTTTACA
GCTGGCTCCAATTTCAACCACGTGGTACAGAATGAAAAATCAGATACACATACTCTGGTG
ACCAGTGGAGTGTACGCTTGGTTTCGGCATCCTTCTTACGTCGGGTGGTTTTACTGGAGT
ATTGGAACTCAGGTGATGCTGTGTAACCCCATCTGCGGCGTCAGCTATGCCCTGACAGTG
TGGCGATTCTTCCGCGATCGAACAGAAGAAGAAGAAATCTCACTAATTCACTTTTTTGGA
GAGGAGTACCTGGAGTATAAGAAGAGGGTGCCCACGGGCCTGCCTTTCATAAAGGGGGTC
AAGGTGGACCTGTGA

Clone variation with respect to NM_012405.3
>OriGene 5' read for NM_012405 unedited
NCGTTCGAGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCGCCGC
CCGCTAGTCCGCCGCCCGGCGCCATGGCGGGCTGCGCGGCGCGGGCTCCGCCGGGCTCTG
AGGCGCGTCTCAGCCTCGCCACCTTCCTGCTGGGCGCCTCGGTGCTCGCGCTGCCGCTGC
TCACGCGCGCCGGCCTGCAGGGCCGCACCGGGCTGGCGCTCTACGTGGCCGGGCTCAACG
CGCTGCTGCTGCTGCTCTATCGGCCGCCTCGCTACCAGATAGCCATCCGAGCTTGTTTCC
TGGGGTTTGTGTTCGGCTGCGGCACGCTGCTAAGTTTTAGCCAGTCTTCTTGGAGTCACT
TTGGCTGGTACATGTGCTCCCTGTCATTGTTCCACTATTCTGAATACTTGGTGACAGCAG
TCAATAATCCCAAAAGTCTGTCCTTGGATTCCTTTCTCCTGAATCACAGCCTGGAGTATA
CAGTAGCTGCTCTTTCTTCTTGGTTAGAGTTCACACTTGAAAATATCTTTTGGCCAGAAC
TGAAGCAGATTACCTGGCTCAGTGTCACAGGGCTGCTGATGGTGGTCTTCGGAGAATGTC
TGAGGAAGGCGGCCATGTTTACAGCTGGCTCCAATTTCAACCACGTGGTACAGAATGAAA
AATCAGATACACATACTCTGGTGACCAGTGGAGTGTACGCTTGGTTTCGGCATCCTTCTT
ACGTCGGGTGGTTTTACTGGAGTATTGGAACTCANGTGATGCTGTGTAACCNCATCTGCG
GCGTCAGCTATGCCCTGACAGTGTGGCGATTCTTCCGCGATCGAACAGAAGAAGAAGAAA
TCTCACTAATTCACTTTTTTGGAGAGGAGTACCTGGAGTATAAGAAGAGGGTGCCCACGG
GCCTGCCTTTCATAAGGGG
>OriGene 3' read for NM_012405 unedited
TTAAATAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTCAAAAAATAAGTCATATTTCGGAGTTGAAAAAAGGCTTTTTTGAACATACTTTTT
AGGGTTCGGGCCCAATTAACCAAATGTCGGCCCTTTTTTGGGGAGCTTTAAAACAGAACA
TTTTTTTTTGGGGGCCCCCCCACATTTGAGGATAAAAAACATTTGAAATTGTTTTTTTAA
GCCCCAAAAACCCCCCGGGGAGGGAGGGGGTTTTGACCCCTTTCCACAAATGGGGAGGTC
TAGGCCCGGAAAGGTTTAGGGGCTCCCCACAGGCCGGGGGGGGGGGCAACAAAGGGGGGC
TTTGGCCCCGCCCCAACCCCGCCTTAAAATTAACACTTTTTAAGGGCCCCCGAAACACTG
GAGGGGAGGTTCTCACCCTGAGGCCAATTTCCTGTCTATTCCAAAATGAACCCAGCGTCC
CACCTCTTAAAGCTTCGAGGGGGGACTAAAAGCCAATTTACTCTCCCAAACTTGCAAAAC
AAACATACGGGCTGAATCTTATTGGGGACTATTGGCAGTATTAAAAATTATTTTTGAAAG
CCAATCGGCTTGGATTTGAAGTTGTATATGCCCAAATCCCTTTGGAAAACAGTTTGAAAT
GACTGGAGGGACAATGTTATTTTGAGAACAAAAACAGAAAAACAAAATTCTGAGACTGGC
CTTGAAATTGGAAAATTAAGCTATGGTAAAAGGAANATCAAATCCCAATAAACGGGAAAC
ACCCCCTTCTAAAGGTTTAAGGCCCCCCCCAGGGCAAGCCTTTCAGTAAGCCCTTTCTTG
GAACAAAAA
Restriction Sites NotI-NotI     
ACCN NM_012405
Insert Size 2650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012405.2, NP_036537.1
RefSeq Size 3547 bp
RefSeq ORF 855 bp
Locus ID 23463
UniProt ID O60725
Domains ICMT
Protein Families Transmembrane
Gene Summary This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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