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SPDEF (NM_012391) Human Untagged Clone
CAT＃: SC115408
SPDEF (untagged)-Human SAM pointed domain containing ets transcription factor (SPDEF)
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bA375E1.3; PDEF |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_012391 edited
GAATTCGGCACGAGGCTGACTTCCTCCCAGCACATTCCTGCACTCTGCCGTGTCCACACT GCCCCACAGACCCAGTCCTCCAAGCCTGCTGCCAGCTCCCTGCAAGCCCCTCAGGTTGGG CCTTGCCACGGTGCCAGCAGGCAGCCCTGGGCTGGGGGTAGGGGACTCCCTACAGGCACG CAGCCCTGAGACCTCAGAGGGCCACCCCTTGAGGGTGGCCAGGCCCCCAGTGGCCAACCT GAGTGCTGCCTCTGCCACCAGCCCTGCTGGCCCCTGGTTCCGCTGGCCCCCCAGATGCCT GGCTGAGACACGCCAGTGGCCTCAGCTGCCCACACCTCTTCCCGGCCCCTGAAGTTGGCA CTGCAGCAGACAGCTCCCTGGGCACCAGGCAGCTAACAGACACAGCCGCCAGCCCAAACA GCAGCGGCATGGGCAGCGCCAGCCCGGGTCTGAGCAGCGTATCCCCCAGCCACCTCCTGC TGCCCCCCGACACGGTGTCGCGGACAGGCTTGGAGAAGGCGGCAGCGGGGGCAGTGGGTC TCGAGAGACGGGACTGGAGTCCCAGTCCACCCGCCACGCCCGAGCAGGGCCTGTCCGCCT TCTACCTCTCCTACTTTGACATGCTGTACCCTGAGGACAGCAGCTGGGCAGCCAAGGCCC CTGGGGCCAGCAGTCGGGAGGAGCCACCTGAGGAGCCTGAGCAGTGCCCGGTCATTGACA GCCAAGCCCCAGCGGGCAGCCTGGACTTGGTGCCCGGCGGGCTGACCTTGGAGGAGCACT CGCTGGAGCAGGTGCAGTCCATGGTGGTGGGCGAAGTGCTCAAGGACATCGAGACGGCCT GCAAGCTGCTCAACATCACCGCAGATCCCATGGACTGGAGCCCCAGCAATGTGCAGAAGT GGCTCCTGTGGACAGAGCACCAATACCGGCTGCCCCCCATGGGCAAGGCCTTCCAGGAGC TGGCGGGCAAGGAGCTGTGCGCCATGTCGGAGGAGCAGTTCCGCCAGCGCTCGCCCCTGG GTGGGGATGTGCTGCACGCCCACCTGGACATCTGGAAGTCAGCGGCCTGGATGAAAGAGC GGACTTCACCTGGGGCGATTCACTACTGTGCCTCGACCAGTGAGGAGAGCTGGACCGACA GCGAGGTGGACTCATCATGCTCCGGGCAGCCCATCCACCTGTGGCAGTTCCTCAAGGAGT TGCTACTCAAGCCCCACAGCTATGGCCGCTTCATTAGGTGGCTCAACAAGGAGAAGGGCA TCTTCAAAATTGAGGACTCAGCCCAGGTGGCCCGGCTGTGGGGCATCCGCAAGAACCGTC CCGCCATGAACTACGACAAGCTGAGCCGCTCCATCCGCCAGTATTACAAGAAGGGCATCA TCCGGAAGCCAGACATCTCCCAGCGCCTCGTCTACCAGTTCGTGCACCCCATCTGAGTGC CTGGCCCAGGGCCTGAAACCCGCCCTCAGGGGCCTCTCTCCTGCCTGCCCTGCCTCAGCC AGGCCCTGAGATGGGGGAAAACGGGCAGTCTGCTCTGCTGCTCTGACCTTCCAGAGCCCA AGGTCAGGGAGGGGCAACCAACTGCCCCAGGGGGATATGGGTCCTCTGGGGCCTTCGGGA CCCTGGGGCAGGGGTGCTTCCTCCTCAGGCCCAGCTGCTCCCCTGGAGGACAGAGGGAGA CAGGGCTGCTCCCCAACACCTGCCTCTGACCCCAGCATTTCCAGAGCAGAGCCTACAGAA GGGCAGTGACTCGACAAAGGCCACAGGCAGTCCAGGCCTCTCTCTGCTCCATCCCCCTGC CTCCCATTCTGCACCACACCTGGCATGGTGCAGGGAGACATCTGCACCCCTGAGTTGGGC AGCCAGGAGTGCCCCCGGGAATGGATAATAAAGATACTAGAGAACTGAAAAAAAAAAAAA AAAAAAAACTCGAC >OriGene 5' read for NM_012391 unedited
GTTCAAAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGACTTCCT CCCAGCACATTCCTGCACTCTGCCGTGTCCACACTGCCCCACAGACCCAGTCCTCCAAGC CTGCTGCCAGCTCCCTGCAAGCCCCTCAGGTTGGGCCTTGCCACGGTGCCAGCAGGCAGC CCTGGGCTGGGGGTAGGGGACTCCCTACAGGCACGCAGCCCTGAGACCTCAGAGGGCCAC CCCTTGAGGGTGGCCAGGCCCCCAGTGGCCAACCTGAGTGCTGCCTCTGCCACCAGCCCT GCTGGCCCCTGGTTCCGCTGGCCCCCCAGATGCCTGGCTGAGACACGCCAGTGGCCTCAG CTGCCCACACCTCTTCCCGGCCCCTGAAGTTGGCACTGCAGCAGACAGCTCCCTGGGCAC CAGGCAGCTAACAGACACAGCCGCCAGCCCAAACAGCAGCGGCATGGGCAGCGCCAGCCC GGGTCTGAGCAGCGTATCCCCCAGCCACCTCCTGCTGCCCCCCGACACGGTGTCGCGGAC AGGCTTGGAGAAGGCGGCAGCGGGGGCAGTGGGTCTCGAGAGACGGGACTGGAGTCCCAG TCCACCCGCCACGCCCGAGCAGGGCCTGTCCGCCTTCTACCTCTCCTACTTTGACATGCT GTACCCTGAGGACAGCAGCTGGGCAGCCAAGGCCCCTGGGGCCAGCAGTCGGGAGGAGCC ACCTGAGGAGCCTGAGCAGTGCCCGGTCATTGACAGCCAAGCCCCAGCGGGCAGCCTGGA CTTGGTGCCCGGCGGGCTGACCTTGGAGGAGCACTCGCTGGAGCAGGTGCCATTCATGGG TGGTGGGCGAAGTGCTCAAGGACTCGAGACGGCCTGCAAGCTGCTAAAATTCACCGCAGT TCCATGGACTGGAGCCCCACCATGT >OriGene 3' read for NM_012391 unedited
ACGCGGCCGCATTTTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTACAGTTCTCTAGTAT CTTTATTATCCATTCCCGGGGGCACTCCTGGCTGCCCAACTCAGGGGTGCAGATGTCTCC CTGCACCATGCCAGGTGTGGTGCAAAATGGGAGGCAGGGGGATGGAGCAGAGAGAGGCCT GGACTGCCTGTGGCCTTTGTCGAGTCACTGCCCTTCTGTAGGCTCTGCTCTGGAAATGCT GGGGTCAAAGGCAGGTGTTGGGGAGCAGCCCTGTCTCCCTCTGTCCTCCAGGGGAGCAGC TGGGCCTGAGGAGGAAGCACCCCTGCCCCAGGGTCCCGAAGGCCCCAGAGGACCCATATC CCCCTGGGGCAGTTGGTTGCCCCTCCCTGACCTTGGGCTCTGGAAGGTCAGAGCAGCAGA GCAGACTGCCCGTTTTCCCCCATCTCAGGGCCTGTCTGAGGCAGGGCAGGCAGGAGAGAG GCCCCTGAGGGCGGGTTTCAGGCCCTGGGCCAGGCACTCAGATGGGGTGCACGAACTGGT AAACGAGGCGCTGGGAGATGCCTGGCTTCCGGATGATGCCCTTCTTGTAATACTGGCGGA TGGAGCGGCTCACCTTGCTCCACTTTATGGTGGGACGGTTCCTGCGGATGCCCCACAGCC GGGCCACCTGGGCTGAGTCCTCAATTTTGAAGATGCCCTTCTCCTTGTTGAGCCATCTAT TGAAGCGGCCCTAGCTGCGCGGCTTGAGTACAACTCCCTGTAGAACTGCCCAGGTGGATG GGCTCGCCGAAACATGATGAGTCACCTCGCTGCCGGTCCAAGTTTCTCACTGGACGAAGG AAAGTATTGATTGCCCCAGGGGAACCCCGTTCTTTTTTCCAGCCGCTGACTTCCAATGTA CAGGCGGGCGCCCCAACATTCCCACCGAGCGGACGCCTGTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012391 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012391.1, NP_036523.1 |
| RefSeq Size | 1894 bp |
| RefSeq ORF | 1008 bp |
| Locus ID | 25803 |
| UniProt ID | O95238 |
| Domains | ETS, SAM_PNT |
| Protein Families | Transcription Factors |
| Gene Summary | The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
,Aspen Workman, James Eudy, Lynette Smith, Leticia Frizzo da Silva, Devis Sinani, Halie Bricker, Emily Cook, Alan Doster, and Clinton Jones,
J. Virol., Mar 2012; 86: 2459 - 2473.
[SPDEF]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205688 | SPDEF (Myc-DDK-tagged)-Human SAM pointed domain containing ets transcription factor (SPDEF) |
CNY 5,488.00 |
|
| RC205688L1 | Lenti ORF clone of Human SAM pointed domain containing ets transcription factor (SPDEF), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205688L2 | Lenti ORF clone of Human SAM pointed domain containing ets transcription factor (SPDEF), mGFP tagged |
CNY 7,888.00 |
|
| RC205688L3 | Lenti ORF clone of Human SAM pointed domain containing ets transcription factor (SPDEF), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205688L4 | Lenti ORF clone of Human SAM pointed domain containing ets transcription factor (SPDEF), mGFP tagged |
CNY 7,888.00 |
|
| RG205688 | SPDEF (tGFP-tagged) - Human SAM pointed domain containing ets transcription factor (SPDEF) |
CNY 7,088.00 |
