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ATG4B (NM_013325) Human Untagged Clone
CAT＃: SC115281
ATG4B (untagged)-Human ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 1
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APG4B; AUTL1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115281 sequence for NM_013325 edited (data generated by NextGen Sequencing)
ATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCT GAGACCTCAGAGCCCGTTTGGATACTGGGTAGAAAATACAGCATTTTCACAGAAAAGGAC GAGATCTTGTCTGATGTGGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCCAGCC ATTGGGGGGACAGGCCCCACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAG ATGATCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACACAA AGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGAC AGTTACTACTCCATTCACCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTCCATAGGC CAGTGGTACGGGCCCAACACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACG TGGAGCTCCTTGGCGGTCCACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGA AGGTTGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCGAC CGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGA CCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGCTCACGGACATCAACGAGGCCTACGTG GAGACGCTGAAGCACTGCTTCATGATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCC AACAGCGCCCACTACTTCATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACCCCCAC ACCACGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGCTTCCAC TGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGAGCTTGACCCGTCCATCGCTGTGGGG TTTTTCTGTAAGACTGAAGATGACTTCAATGATTGGTGCCAGCAAGTCAAAAAGCTGTCT CTGCTTGGAGGTGCCCTGCCCATGTTTGAGCTGGTGGAGCAGCAGCCTTCACATCTGGCC TGCCCCGACGTCCTGAACCTGTCCCTAGATTCTTCTGATGTAGAGCGACTGGAAAGATTC TTCGACTCAGAAGATGAAGACTTTGAAATCCTGTCCCTTTGA Clone variation with respect to NM_013325.4 1061 t=>a >OriGene 5' read for NM_013325 unedited
CTATAGGGGCGGCCGCGAATTCGCACGAGGCCGGACTGGGAAGATGGACGCAGCTACTCT GACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCTGAGACCTCAGAGCCCGT TTGGATACTGGGTAGAAAATACAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGT GGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCCAGCCATTGGGGGGACAGGCCC CACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAGATGATCTTTGCCCAAGC CCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACACAAAGGAAGAGGCAGCCAGA CAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGACAGTTACTACTCCATTCA CCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCCAA CACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGCGGT CCACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGAAGGTTGTGCAGGACCAG CGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCGACCGGCACTGCAACGGATT CCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGACCCCTGGTACTTCTCAT TCCCCTGCGCCTGNNGCTCACGGACATCAACGAGGCCTACGTGGAGACGCTGAAGCACTG CTTCATGATGCCCNCAGTCCTGGGGCGTCATCGGGAGGGAAGCCCNACAGCGCCACTACT TCATCGGT >OriGene 3' read for NM_013325 unedited
CCCTGAAGGGTNATTTATCTCCTTACAACCACANAACCGGCGCCTGGGAGCAGTGGGACT GAGGCCCAGGTGCTAACACGGGGCTGGCAGTGTCGAGAGAACGCTCTGGAAGCTCCTAAC AGACGGCTCCGCGTGCGGATGCACAGGCCCTGACGGGCACTCTGAGCTGGGCAGTCTGAC ACCAAGCAGTAAGGCCTCCCGGGCAGCGCAGCCTCAGTCCACGAGCACAGCGGGTGGCCC TCTGGGGGGAGGCAGCACGGGGCGCAGCCCTCGGCAGGCGAGCGGGCGGGATGGATGAAA CGCAGCGGCACCAGGAGCCCCAGGCTCTCACAGGTGCCACCCCCGACCCCAGGATTTTCA AAGGGACAGGATTTCAAAGTCTTCATCTTCTGAGTCGAAGAATCTTTCCAGTCGCTCTAC ATCAGAAGAATCTAGGGACAGGTTCAGGACGTCGGGGCAGGCCAGATGTGAAGGCTGCTG CTCCACCAGCTCAAACATGGGCAGGGCACCTCCAAGCAGAGACAGCTTTTTGACTTGCTG GCACCAATCATTGAAGTCATCTTCAGTCTTACAGAAAAACCCCACAGCGATGGACGGGTC AAGCTCCGCGATGCTCATGCGGCACGGCGGGTGCTGGCAGTGGAAGCTCTCGTCCGGGAT GAAGCAGCCATCAGTGGGCTCCACGGCTGGCTGCGTGGTGTGGGGGTCCAGGTAGATGAG CTCCTCACCAACGTAGCCGATGAAGTAGTGGGCGCTGTTGGGCTTCCCTCCGATGACGCC CAGGGACTGGGGCATCATGAAGCAGTGCTTCAGCGNTCTCACGTAGGCCTCGTTGATGTC CGTGAGCCCCAGGCGCATGGGAATGAGAAGTACCAGGGGTCTCCATGGCGACAGCCTGTT GGTGACCTCAGCTCCGGCAAGGAATCCGTTGCAATGCCGGTCGGATCTGCAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013325 |
| Insert Size | 1600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013325.4, NP_037457.3 |
| RefSeq Size | 2892 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 23192 |
| UniProt ID | Q9Y4P1 |
| Domains | Peptidase_C54 |
| Protein Families | Protease |
| Protein Pathways | Regulation of autophagy |
| Gene Summary | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the predominant transcript and it encodes the longer isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200289 | ATG4B (Myc-DDK-tagged)-Human ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 1 |
CNY 3,656.00 |
|
| RC200289L1 | Lenti ORF clone of Human ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200289L2 | Lenti ORF clone of Human ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200289L3 | Lenti ORF clone of Human ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200289L4 | Lenti ORF clone of Human ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200289 | ATG4B (tGFP-tagged) - Human ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 1 |
CNY 5,256.00 |
