Apc2 (ANAPC2) (NM_013366) Human Untagged Clone
CAT#: SC115268
ANAPC2 (untagged)-Human anaphase promoting complex subunit 2 (ANAPC2)
CNY 6,032.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | APC2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC115268 sequence for NM_013366 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCAGTTGTGGTGGCGGAGGGGGACAGCGACTCCCGGCCCGGACAGGAGTTG TTAGTGGCCTGGAACACCGTGAGCACCGGCCTGGTGCCGCCGGCTGCGCTGGGGCTGGTG TCTTCCCGGACCAGCGGTGCAGTCCCGCCAAAGGAAGAGGAGCTCCGGGCGGCGGTGGAG GTTCTGAGGGGCCACGGGCTACACTCGGTCCTGGAGGAGTGGTTCGTGGAGGTGCTGCAG AACGATCTGCAGGCCAACATCTCCCCTGAGTTCTGGAATGCCATCTCCCAATGCGAGAAC TCTGCGGATGAGCCCCAGTGCCTTTTGCTACTCCTTGACGCTTTTGGCCTGCTGGAGAGC CGCCTGGATCCCTACCTGCGTAGCCTAGAGCTGCTGGAGAAATGGACTCGCCTGGGCTTG CTGATGGGCACTGGTGCTCAGGGGCTGCGAGAAGAAGTCCACACTATGTTGCGCGGAGTC TTGTTCTTTAGCACCCCCAGAACCTTCCAAGAGATGATCCAGCGTCTGTATGGGTGCTTC TTGAGAGTCTATATGCAGAGTAAGAGGAAGGGGGAAGGGGGCACAGACCCGGAACTGGAA GGGGAGCTGGACAGCCGGTATGCCCGTCGCCGGTACTACCGGCTCCTGCAGAGCCCGCTG TGTGCAGGGTGCAGCAGTGACAAGCAACAGTGCTGGTGTCGCCAGGCTCTGGAGCAGTTC CATCAGCTCAGCCAGGTCTTACACAGGCTCAGTCTGCTGGAGCGGGTCAGTGCCGAGGCT GTGACCACCACCCTGCACCAGGTGACCCGGGAGAGGATGGAGGACCGTTGCCGGGGCGAG TACGAGCGCTCCTTCCTGCGTGAGTTCCACAAGTGGATCGAGCGGGTGGTCGGCTGGCTC GGCAAGGTGTTCCTGCAGGACGGCCCCGCCAGGCCCGCATCTCCCGAGGCCGGCAACACC CTGCGCCGCTGGCGCTGCCACGTGCAAAGGTTCTTCTACCGCATCTACGCCAGCCTGCGC ATCGAGGAGCTCTTCAGCATCGTCCGAGACTTCCCAGACTCCCGGCCAGCCATCGAGGAC CTCAAGTACTGCCTGGAGAGGACGGACCAGAGGCAGCAGCTGCTCGTGTCCCTCAAGGCT GCCCTGGAGACTCGGCTCCTGCATCCAGGCGTCAACACGTGTGACATCATCACCCTCTAT ATCTCTGCCATCAAGGCGCTGCGCGTGCTGGACCCTTCCATGGTCATCCTGGAGGTGGCC TGTGAGCCTATCCGCCGCTACCTGAGGACGCGGGAGGACACAGTGCGGCAGATTGTGGCT GGGCTGACGGGGGACTCGGACGGGACAGGGGACCTGGCTGTTGAGCTGTCCAAGACCGAC CCGGCGAGCCTGGAGACAGGCCAGGACAGTGAGGATGACTCAGGCGAGCCAGAGGACTGG GTCCCGGACCCTGTGGATGCCGATCCAGGGAAGTCGAGCTCCAAGCGGCGTTCATCGGAC ATCATCAGCCTGCTGGTCAGCATCTACGGCAGCAAGGACCTCTTCATCAATGAGTACCGC TCGCTGCTGGCCGACCGCCTGCTGCACCAGTTCAGCTTCAGCCCCGAGCGGGAGATCCGC AACGTGGAGCTGCTGAAGCTGCGCTTTGGCGAGGCCCCAATGCACTTCTGTGAAGTCATG CTGAAGGACATGGCGGACTCCCGCCGCATCAATGCCAACATCCGGGAGGAGGATGAGAAG CGGCCAGCAGAGGAGCAGCCACCGTTCGGGGTCTACGCTGTCATCCTGTCCAGTGAGTTC TGGCCGCCCTTCAAGGACGAGAAGCTGGAGGTCCCCGAGGATATCAGGGCAGCCCTGGAG GCTTACTGCAAGAAGTATGAGCAGCTCAAGGCCATGCGGACCCTCAGTTGGAAGCACACC CTGGGCCTGGTGACCATGGACGTGGAGCTGGCCGACCGCACGCTGTCTGTGGCGGTCACC CCAGTACAGGCGGTGATCTTGCTGTATTTTCAGGACCAAGCCAGCTGGACCCTGGAGGAA CTGAGCAAGGCGGTGAAGATGCCCGTGGCGCTGCTGCGGCGGCGGATGTCCGTGTGGCTG CAGCAGGGTGTGCTGCGTGAGGAGCCCCCCGGCACCTTCTCTGTCATTGAGGAGGAGCGG CCTCAGGACCGGGACAACATGGTGCTCATTGACAGTGACGACGAGAGCGACTCCGGCATG GCCTCCCAGGCCGACCAGAAGGAGGAGGAGCTGCTGCTCTTCTGGACGTACATCCAGGCC ATGCTGACCAACCTGGAGAGCCTCTCACTGGATCGTATCTACAACATGCTCCGCATGTTT GTGGTGACTGGGCCTGCACTGGCCGAGATTGACCTGCAGGAGCTGCAGGGCTACCTGCAG AAGAAGGTGCGGGACCAGCAGCTCGTCTACTCGGCCGGCGTCTACCGCCTGCCCAAGAAC TGCAGCTGA Clone variation with respect to NM_013366.3 >OriGene 5' read for NM_013366 unedited
TTGAGGGATTTTGGAATACGATCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCG AGCGAATCTCGGAGTCGGTGGGTGCAGATGGCGGCGGCAGTTGTGGTGGCGGAGGGGGAC AGCGACTCCCGGCCCGGACAGGAGTTGTTAGTGGCCTGGAACACCGTGAGCACCGGCCTG GTGCCGCCGGCTGCGCTGGGGCTGGTGTCTTCCCGGACCAGCGGTGCAGTCCCGCCAAAG GAAGAGGAGCTCCGGGCGGCGGTGGAGGTTCTGAGGGGCCACGGGCTACACTCGGTCCTG GAGGAGTGGTTCGTGGAGGTGCTGCAGAACGATCTGCAGGCCAACATCTCCCCTGAGTTC TGGAATGCCATCTCCCAATGCGAGAACTCTGCGGATGAGCCCCAGTGCCTTTTGCTACTC CTTGACGCTTTTGGCCTGCTGGAGAGCCGCCTGGATCCCTACCTGCGTAGCCTAGAGCTG CTGGAGAAATGGACTCGCCTGGGCTTGCTGATGGGCACTGGTGCTCAGGGGCTGCGAGAA GAAGTCCACACTATGTTGCTCGGAGTCTTGTTCTTTAGCACCCCCAGAACCTTCCAAGAG ATGATCCAGCGTCTGTATGGGTGCTTCTTGAGAGTCTATATGCAGAGTAAGAGGAAGGGG GAAGGGGGCACAGACCCGGGACTGGAAGGGGAGCTGGACCAGCCGTTTGCCCGTCGCCCG TACTACCGACTCCTGCAGAGCCCGCTGTGTGCAGGCTGCCACATTGACCAACCACAGTGC TGGGTGTCGCCAGCTCTGGAGCAGTTCCTCAGCTTAACCAGGTCTTACACAGGCTAATTC TGCTGGAACAGCTCAATGCCGCAGCTTGGACCCCACCCTTGGACCAGTGACCCGGTGAAA GGATGAAGGAACCTTTGCCGGGGCGAGATCCAACCTTCCT >OriGene 3' read for NM_013366 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATGATCTGACTTGCTTTAATCT GCACACTGCGGGGGTGGGGGTGGGCATGCTCCTCAGCAGTGCATTCTGGGACACGGGCGG GCGGGGGCTGGCACGGGAGGACGAGAGCACCTGCAGGGCAGCGCCTGGCGGGCGGGCGGG CGGGCGGGCGATGTGTCAGCTGCAGTTCTTGGGCAGGCGGTAGACGCCGGCCGAGTAGAC GAGCTGCTGGTCCCGCACCTTCTTCTGCAGGTAGCCCTGCAGCTCCTGCAGGTCAATCTC GGCCAGTGCAGGCCCAGTCACCACAAACATGCGGAGCATGTTGTAGATACGATCCAGTGA GAGGCTCTCCAGGTTGGTCAGCATGGCCTGGATGTACGTCCAGAAGAGCAGCAGCTCCTC CTCCTTCTGGTCGGCCTGGGAGGCCATGCCGGAGTCGCTCTCGTCGTCACTGTCAATGAG CACCATGTTGTCCCGGTCCTGAGGCCGCTCCTCCTCAATGACAGAGAAGGTGCCGGGGGG CTCCTCACGCAGCACACCCTGCTGCAGCCACACGGACATCCGCCGCCGCAGCAGCGCCAC GGGCATCTTCACCGCCTTGCTCAGTTCCTCCAGGGTCCAGCTGGCTTGGTCCTGAAAATA CAGCAAGATCACCGCCTGTACTGGGGTGACCGCACAGACAGCGTGCGGTCGGCCAGCTCC ACGTCCATGTCCACAGGCCCAGGTGTGCTTCCAACTGAGTCCGCATGCCCTGAGCTGCTA TACTTCTGCAGTAGCCTCAGGCCTGCCTGAATCCTGGGGACTCCACTTTCGTCCTGAGGC CGGCCNACTACTTCCAGAGACAGGTAACCCCAACGTGGTGTCCTTGTTGCCGGTTATCTT CTCGGATGTGCTTGAGCGCGGAGTCCCAGTCTTAATGATTCACAAGCCTGGCTCCCN |
Restriction Sites | NotI-NotI |
ACCN | NM_013366 |
Insert Size | 2800 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_013366.3, NP_037498.1 |
RefSeq Size | 2733 bp |
RefSeq ORF | 2469 bp |
Locus ID | 29882 |
UniProt ID | Q9UJX6 |
Domains | CULLIN |
Protein Families | Druggable Genome |
Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
Gene Summary | A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207539 | ANAPC2 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 2 (ANAPC2) |
CNY 6,024.00 |
|
RC207539L1 | Lenti ORF clone of Human anaphase promoting complex subunit 2 (ANAPC2), Myc-DDK-tagged |
CNY 8,424.00 |
|
RC207539L2 | Lenti ORF clone of Human anaphase promoting complex subunit 2 (ANAPC2), mGFP tagged |
CNY 7,700.00 |
|
RC207539L3 | Lenti ORF clone of Human anaphase promoting complex subunit 2 (ANAPC2), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC207539L4 | Lenti ORF clone of Human anaphase promoting complex subunit 2 (ANAPC2), mGFP tagged |
CNY 7,700.00 |
|
RG207539 | ANAPC2 (tGFP-tagged) - Human anaphase promoting complex subunit 2 (ANAPC2) |
CNY 7,624.00 |
|
SC324311 | ANAPC2 (untagged)-Human anaphase promoting complex subunit 2 (ANAPC2) |
CNY 6,032.00 |