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Isoleucyl tRNA synthetase (IARS) (NM_013417) Human Untagged Clone
CAT＃: SC115260
IARS (untagged)-Human isoleucyl-tRNA synthetase (IARS), transcript variant long
CNY 8,792.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_013417, the custom clone sequence may differ by one or more nucleotides
ATGCTTCAACAAGTTCCAGAAAACATAAATTTTCCTGCTGAAGAAGAGAAAATCTTGGAGTTTTGGACTG AATTTAATTGTTTTCAGGAATGCTTAAAGCAATCAAAACATAAACCAAAATTTACCTTCTATGATGGTCC TCCTTTTGCAACTGGACTGCCTCACTATGGACATATACTTGCGGGTACAATTAAAGATATAGTTACAAGA TATGCTCACCAGAGTGGGTTTCATGTTGACAGAAGATTTGGATGGGATTGCCATGGCTTACCTGTGGAAT ATGAAATTGATAAGACACTGGGAATCAGAGGACCAGAGGATGTGGCCAAAATGGGGATTACAGAGTATAA CAATCAGTGCCGAGCAATTGTGATGAGATATTCTGCTGAGTGGAAGTCTACTGTTAGCAGACTTGGCCGA TGGATTGACTTTGACAATGACTATAAAACTCTGTATCCACAATTCATGGAATCAGTCTGGTGGGTCTTCA AACAACTCTATGATAAAGGCCTTGTTTATAGAGGTGTGAAAGTCATGCCCTTCTCTACGGCATGTAACAC TCCACTTTCCAACTTCGAGTCACACCAGAATTATAAGGATGTTCAAGATCCTTCAGTATTTGTAACTTTC CCTTTGGAAGAAGATGAAACTGTATCTTTAGTTGCTTGGACAACCACTCCCTGGACTCTACCTAGTAACC TTGCTGTGTGTGTTAATCCAGAAATGCAATATGTGAAAATTAAAGATGTTGCCAGAGGACGATTACTCAT TTTAATGGAAGCCAGATTGTCAGCCCTCTATAAATTGGAGAGTGACTATGAGATCCTTGAAAGATTTCCT GGTGCCTATCTTAAAGGCAAGAAGTACAGGCCCCTGTTTGACTATTTCCTGAAGTGTAAAGAGAATGGCG CTTTCACTGTGCTTGTTGACAACTATGTGAAGGAAGAAGAAGGCACAGGGGTTGTCCACCAAGCTCCTTA CTTCGGTGCTGAGGACTATCGGGTCTGTATGGACTTTAACATTATTCGGAAAGACTCACTCCCTGTTTGC CCTGTGGATGCTTCAGGCTGCTTCACAACGGAGGTGACAGATTTCGCAGGACAGTATGTGAAGGATGCTG ACAAAAGTATCATCAGGACTTTGAAGGAACAAGGCCGACTTCTGGTTGCCACCACCTTCACTCACAGCTA CCCTTTTTGCTGGAGATCAGACACTCCTCTAATTTACAAAGCAGTGCCCAGCTGGTTTGTGCGAGTGGAG AACATGGTGGACCAGCTCCTAAGGAACAATGACCTGTGCTACTGGGTCCCAGAGTTGGTACGAGAAAAAC GATTTGGAAATTGGCTGAAAGATGCACGTGACTGGACAATTTCCAGAAACAGATACTGGGGCACCCCCAT CCCACTGTGGGTCAGCGATGACTTTGAGGAGGTGGTATGCATTGGGTCAGTGGCGGAACTTGAAGAACTG TCAGGAGCAAAGATCTCAGATCTCCACAGAGAGAGTGTTGACCACCTGACCATTCCTTCACGCTGTGGGA AGGGATCCTTGCACCGCATCTCTGAAGTGTTTGACTGTTGGTTTGAGAGTGGCAGCATGCCCTATGCTCA GGTTCATTACCCGTTTGAAAACAAGAGGGAGTTTGAGGATGCTTTTCCTGCAGATTTCATTGCCGAGGGC ATCGACCAAACCAGAGGATGGTTTTATACCCTGCTGGTGCTGGCCACGGCCCTCTTTGGACAACCGCCTT TCAAGAACGTAATTGTGAATGGGCTTGTCCTGGCAAGTGATGGCCAAAAAATGAGCAAACGGAAAAAGAA TTATCCAGATCCAGTTTCCATCATCCAGAAGTATGGTGCTGATGCCCTCAGATTATATCTGATTAACTCC CCTGTGGTGAGAGCAGAAAACCTCCGCTTTAAAGAAGAGGGTGTGCGGGACGTCCTTAAGGATGTACTGC TCCCATGGTACAATGCCTATCGCTTCTTAATCCAGAACGTTCTGAGGCTCCAGAAGGAGGAAGAAATAGA ATTTCTCTACAATGAGAACACGGTTAGAGAAAGCCCCAACATTACAGACCGGTGGATCCTGTCCTTCATG CAGTCTCTCATTGGCTTCTTTGAGACTGAAATGGCAGCTTATAGGCTTTATACTGTGGTGCCTCGCCTGG TCAAGTTTGTAGATATTCTGACCAATTGGTATGTTAGAATGAACCGCAGAAGATTAAAGGGTGAAAATGG GATGGAGGATTGTGTCATGGCCCTAGAAACCTTGTTTAGTGTTCTGCTTTCTCTTTGCAGACTTATGGCT CCCTACACACCTTTTCTCACTGAATTGATGTACCAGAATCTAAAGGTGCTGATTGACCCTGTTTCTGTTC AGGACAAGGACACACTCAGCATTCACTACCTCATGCTGCCCCGTGTTCGAGAAGAATTGATTGACAAGAA AACAGAGAGTGCAGTATCTCAGATGCAGTCTGTGATTGAACTTGGAAGAGTGATCAGAGACCGAAAAACT ATTCCCATAAAGTATCCTTTGAAAGAAATTGTGGTTATCCATCAAGATCCAGAAGCTCTTAAAGATATCA AGTCTTTGGAGAAGTATATCATTGAGGAACTCAATGTTCGAAAAGTTACACTGTCTACAGATAAAAACAA GTATGGCATTCGGCTAAGGGCAGAACCAGATCACATGGTCCTGGGGAAGCGTCTGAAGGGAGCCTTTAAG GCAGTGATGACGTCCATCAAGCAGTTGAGCAGTGAGGAGCTGGAGCAGTTCCAGAAGACTGGGACCATTG TTGTGGAAGGCCATGAATTGCACGATGAAGACATCCGCCTCATGTACACCTTTGATCAGGCCACAGGTGG GACTGCGCAATTTGAAGCACACTCAGATGCTCAGGCTTTGGTCCTCTTAGATGTCACTCCTGACCAGTCA ATGGTAGATGAAGGAATGGCTCGGGAAGTCATCAATCGCATACAGAAACTTCGCAAAAAGTGCAATCTGG TTCCAACTGATGAAATCACAGTGTACTATAAAGCAAAGTCTGAAGGAACATATCTGAATAGTGTTATTGA AAGCCACACAGAGTTCATATTTACCACCATAAAGGCTCCCTTGAAACCATATCCAGTTTCTCCATCGGAT AAAGTCCTTATTCAAGAAAAAACACAGTTGAAGGGATCTGAACTGGAAATTACACTCACCAGAGGATCTT CCCTTCCTGGTCCTGCTTGTGCATATGTCAATCTTAACATTTGTGCAAATGGCAGTGAACAAGGTGGAGT ATTGCTCCTGGAAAATCCAAAAGGTGACAATAGGTTGGACCTTTTAAAGCTGAAGAGTGTTGTCACTAGC ATTTTTGGTGTGAAAAATACAGAGCTGGCTGTCTTCCATGATGAAACAGAAATACAAAACCAAACTGACT TACTGAGTCTTAGTGGAAAAACACTTTGTGTGACTGCAGGATCGGCTCCCTCTCTGATCAACAGTTCTAG TACTCTTCTTTGTCAGTATATCAACCTACAGCTCCTGAATGCAAAGCCACAAGAGTGTTTAATGGGGACA GTGGGCACTCTCCTGCTTGAAAACCCACTTGGGCAGAATGGACTCACCCACCAAGGTCTTCTGTATGAAG CAGCCAAGGTGTTTGGCCTTCGGAGCAGGAAGCTAAAGCTGTTTCTGAATGAGACCCAAACGCAGGAAAT TACAGAAGACATCCCCGTGAAGACTTTGAATATGAAGACTGTGTATGTTTCTGTGTTACCAACAACAGCA GACTTCTAG >OriGene 5' read for NM_013417 unedited
TCGGCATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACCAGCGTTGCAT CACCTCCGTGCGCCCGGTTGCAGCGTGGACGCCGGATGAGTTGCTTTTAGGCTTGCTGGC CCGCGGGGCTGTCCAGGCACGCGAGGCCCCTCAGCAACAAAATGCTTCAACAAGTTCCAG AAAACATAAATTTTCCTGCTGAAGAAGAGAAAATCTTGGAGTTTTGGACTGAATTTAATT GTTTTCAGGAATGCTTAAAGCAATCAAAACATAAACCAAAATTTACCTTCTATGATGGTC CTCCTTTTGCAACTGGACTGCCTCACTATGGACATATACTTGCGGGTACAATTAAAGATA TAGTTACAAGATATGCTCACCAGAGTGGGTTTCATGTTGACAGAAGATTTGGATGGGATT GCCATGGCTTACCTGTGGAATATGAAATTGATAAGACACTGGGAATCAGAGGACCAGAGG ATGTGGCCAAAATGGGGATTACAGAGTATAACAATCAGTGCCGAGCAATTGTGATGAGAT ATTCTGCTGAGTGGAAGTCTACTGTTAGCAGACTTGGCCGATGGATTGACTTTGACAATG ACTATAAAACTCTGTATCCACAATTCATGGAATCAGTCTGGTGGGTCTTCAAACAACTCT ATGATAAAGGCCTTGTTTATAGAGGTGTGAAAGTCATGCCCTTCTCTACGGCATGTAACA CTCCACTTTCCAACTTCGAGTCACACCAGAATTATAAAGATGTTCAAGATCCTTCAGTAT TTGTAACTTTCCCTTTGGAAGAAGATGAAACTGTATCTTTAGTTGCTTGGACACCACNTC CTGGACTCTACCTAGTACCCTGCTGTGTGTGTTATCCAGAATGCATATGTGAAAATAAAG ATGTGCCAAAGGACGATACTCATTTAATGGAGCCAGATGTAGCCCTT >OriGene 3' read for NM_013417 unedited
CCCCCCCCTTTTACTTGNCGCGCGGCCGCTTTCTACGACCGGTCTTTTTTTTTTTTTTTT TTTTACACCTGAGTTTGTTTATTGTTCTGATTTACTGCAACTTAAATATTCCATCAAATT ATCCAGGACAATCCAGGTGGCAGACAAATATAATATGTCCATTTCATCACGAGGTCTCAA ACAAATTTTAAAAGGCCAGACAATGATATATATACTATGCCATTTAAATCACTTCTATCT TCTGTACTTAAGAACTCACGTATAGAAATAAACTGTGGGCTGAAGTAACATTGTAACCTG CTCCCAACATGACTGCATAGGTGTCTAAGGTTAAGTGTGAACATTACTGCGAGGTCTCAC GTTACTTGACTAATTCCTCTCCATTTGAATTTCAATCCTCGCACTATATTTTACACCCAC CTGTAAGGAACACACCTTCCGCGCGTCCATGTGTGCGCCTATGCGCATGCATGTGTACGG GACTCGTGTCACCCCGCGAACTCCCCCCACCACCCTCCCCCACCAATTTCACCCCCCCAC CTCCCTCTCTCTTCTCCCCTCCTCCTCCCCCCCCACACCCCCCTCTCCTTCTTGTCTCCT TTTACCACCCCCTCCATCCCCTCACCACCCCCCCCCCTCCCCGCCCACACCCTTCCCTCC CTACCCCCCCTCACTCCCCCACCACTCTTCCCCATCCCCCTCACCTCCTCTCCCCCACCA TTCTTTCCTCTTCCTTCCTCCTTCTCCTCCTCCTTCCCTCCCCTCCTTCCCCTCTGTCTC TTATTTCCTCCCCTCCCTCCCCCACCCACTACACCGCCTACTCCACCTCTCCCCAACGTA ATCCACTACTATCCGACCGTACCTCATTCTACCCTCCTCCCAATCCACGTCTAACTTCCC CATATTGTCACTTCCAACCCAACCACCATTATTCACCCCTCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013417 |
| Insert Size | 3890 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013417.1, NP_038203.1 |
| RefSeq Size | 4508 bp |
| RefSeq ORF | 3789 bp |
| Locus ID | 3376 |
| UniProt ID | P41252 |
| Domains | tRNA-synt_1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis |
| Gene Summary | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213208 | IARS (Myc-DDK-tagged)-Human isoleucyl-tRNA synthetase (IARS), transcript variant long |
CNY 8,784.00 |
|
| RC213208L3 | Lenti ORF clone of Human isoleucyl-tRNA synthetase (IARS), transcript variant long, Myc-DDK-tagged |
CNY 14,540.00 |
|
| RC213208L4 | Lenti ORF clone of Human isoleucyl-tRNA synthetase (IARS), transcript variant long, mGFP tagged |
CNY 14,540.00 |
|
| RG213208 | IARS (tGFP-tagged) - Human isoleucyl-tRNA synthetase (IARS), transcript variant long |
CNY 10,384.00 |
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