United States
Germany
Japan
United Kingdom
China
VPRBP (DCAF1) (NM_014703) Human Untagged Clone
CAT＃: SC114916
VPRBP (untagged)-Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 1
CNY 28,980.00
货期*
8周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RIP; VPRBP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_014703 edited
AGCCGTGGTCGCGCTGTGTGTGTGTGTGAGTGGAGAGTGAGTGCGGGACCTGAGGCTGTG TGTGTGTGAACTGTGGCCACTGGCGGGGTCGGGGGGGCAGCGGCGGAGCCGCCTCGGACG GCAAAGCCATGACTACAGTAGTGGTACATGTGGACTCCAAAGCTGAGCTCACTACCCTGC TGGAGCAGTGGGAAAAGGAACATGGCAGTGGGCAGGACATGGTACCTATCCTTACCAGGA TGTCTCAATTGATTGAAAAAGAAACTGAAGAGTATCGTAAAGGGGATCCAGACCCATTTG ATGATCGACATCCTGGTCGAGCTGATCCAGAGTGTATGCTGGGCCACTTGCTGAGAATAC TCTTCAAGAATGATGATTTCATGAAT:::CTGGTGAATGCATATGTGATGACAAGCCGAG AGCCCCCTTTAAACACTGCAGCTTGCAGACTCCTATTAGACATCATGCCAGGGCTGGAAA CTGCTGTCGTCTTTCAAGAAAAGGAGGGAATTGTCGAGAATCTTTTCAAATGGGCCCGAG AGGCCGATCAACCATTGAGGACATATTCTACTGGACTGTTAGGAGGTGCTATGGAAAATC AAGACATTGCTGCCAACTATAGAGATGAAAATTCACAGCTGGTGGCAATAGTGCTTCGAA GACTGAGGGAGCTACAGCTACAGGAAGTGGCTTTGCGGCAGGAAAACAAGCGTCCCAGTC CACGGAAGCTCTCTTCTGAACCCCTTTTGCCTCTGGATGAGGAGGCTGTGGATATGGACT ATGGTGACATGGCTGTAGATGTAGTGGATGGAGACCAAGAGGAAGCTTCTGGAGACATGG AGATCTCCTTTCATCTTGATTCAGGCCACAAGACTAGTAGCAGAGTGAACTCAACAACCA AACCTGAGGATGGAGGATTAAAGAAAAACAAGTCAGCAAAACAGGGTGACAGAGAGAACT TTAGGAAAGCCAAGCAAAAGTTGGGTTTCTCATCTTCTGATCCAGATCGCATGTTTGTTG AGCTGTCTAATAGCAGTTGGTCAGAAATGTCTCCCTGGGTGATTGGCACCAATTATACCC TTTATCCTATGACTCCTGCTATCGAGCAGCGACTCATTCTCCAATATTTGACCCCTCTAG GAGAATATCAGGAGCTACTTCCCATATTCATGCAACTTGGATCACGGGAGCTGATGATGT TCTATATTGACCTGAAGCAAACTAATGATGTCCTGCTTACATTTGAGGCACTAAAGCACC TAGCATCTCTCCTGCTCCATAACAAATTTGCCACAGAATTTGTTGCGCATGGTGGAGTAC AGAAATTACTGGAAATACCTCGTCCTTCTATGGCTGCAACCGGTGTGTCTATGTGTTTGT ATTACCTATCCTACAATCAGGATGCCATGGAAAGAGTTTGCATGCATCCCCACAATGTTC TGTCTGATGTGGTGAACTATACCCTGTGGTTAATGGAGTGTTCTCATGCTTCAGGATGCT GCCATGCTACCATGTTTTTTTCAATTTGCTTCTCATTTCGGGCCGTCTTGGAGCTCTTTG ACCGCTATGATGGTCTTCGTCGTCTGGTGAACTTGATCAGTACTTTGGAGATTCTAAATT TGGAAGATCAGGGTGCACTTCTGAGTGATGATGAAATATTTGCTAGCCGCCAAACTGGGA AACATACCTGCATGGCCTTGCGCAAATACTTTGAGGCTCACCTGGCCATTAAATTGGAAC AAGTGAAGCAGTCACTTCAGAGGACTGAGGGTGGCATTCTTGTCCACCCACAACCCCCGT ACAAGGCATGCTCATATACTCATGAACAGATTGTGGAAATGATGGAATTTTTGATAGAAT ATGGCCCAGCGCAGCTATATTGGGAACCAGCTGAAGTTTTCCTCAAACTTTCTTGTGTGC AACTCTTGTTGCAGCTTATTTCTATTGCCTGCAATTGGAAGACCTATTATGCAAGGAATG ACACTGTGCGCTTTGCTTTGGATGTCCTGGCTATTCTTACTGTGGTGCCAAAAATCCAGC TCCAGTTGGCAGAATCAGTGGACGTGTTGGATGAGGCTGGATCTACAGTCTCTACTGTAG GTATCAGCATTATTTTGGGAGTGGCTGAGGGTGAGTTCTTCATCCATGATGCTGAAATTC AGAAGTCAGCACTTCAGATTATCATCAATTGTGTGTGTGGCCCAGATAACCGAATATCCA GTATTGGTAAATTTATCTCTGGTACTCCTCGGAGAAAGCTGCCTCAGAACCCTAAAAGCA GTGAGCACACCCTGGCCAAGATGTGGAATGTGGTTCAGTCCAACAACGGCATCAAGGTGC TCCTGTCCTTACTGTCCATTAAGATGCCCATCACAGATGCAGACCAAATCCGGGCCCTGG CCTGCAAAGCCCTAGTGGGCCTGTCTCGCAGTAGCACTGTCCGGCAGATCATCAGTAAAC TGCCCCTTTTCAGCAGCTGCCAGATCCAGCAGCTGATGAAGGAGCCTGTGCTGCAGGACA AGCGCAGTGACCATGTCAAGTTCTGCAAGTATGCTGCTGAACTCATTGAACGGGTGTCAG GAAAACCACTTCTCATTGGCACTGATGTTTCCCTAGCACGACTGCAGAAAGCAGATGTTG TTGCCCAGTCAAGGATCTCCTTCCCTGAGAAAGAGCTGCTTTTGTTGATACGAAACCATC TTATTTCTAAAGGGCTTGGAGAAACAGCAACCGTGCTGACAAAAGAGGCTGACCTGCCCA TGACTGCTGCCTCCCATTCTTCTGCCTTTACCCCAGTCACTGCTGCTGCTTCTCCTGTCT CTCTACCCCGAACCCCTCGTATCGCTAATGGCATTGCAACTCGTCTGGGCAGCCATGCTG CTGTGGGTGCCTCTGCGCCTTCTGCCCCTACTGCTCATCCTCAGCCACGGCCCCCCCAGG GTCCGCTAGCTCTGCCCGGCCCATCTTATGCAGGCAACTCCCCTTTGATTGGTAGAATCA GTTTTATCAGAGAGAGGCCATCACCCTGCAATGGCAGGAAAATCAGAGTGTTGCGGCAGA AGTCGGACCATGGTGCCTACAGCCAAAGCCCAGCCATAAAAAAACAGCTGGACAGACATC TTCCTTCCCCACCTACGCTGGACAGTATAATCACAGAGTATCTTAGAGAACAACATGCTC GCTGCAAGAATCCAGTTGCCACCTGCCCACCTTTCTCCCTCTTTACTCCTCACCAATGTC CTGAGCCAAAACAGAGGCGGCAAGCGCCAATAAACTTTACGTCAAGGCTAAACCGCAGGG CATCATTTCCAAAGTATGGAGGGGTGGATGGCGGATGCTTTGATAGGCACCTTATCTTTA GCAGATTCCGTCCTATTTCAGTGTTCCGGGAAGCCAATGAAGATGAGAGTGGCTTCACCT GCTGTGCATTCTCAGCACGGGAGCGGTTCCTGATGCTTGGCACCTGCACAGGGCAGCTGA AGCTCTATAATGTGTTTAGTGGACAGGAGGAGGCCAGCTATAACTGTCACAACTCAGCCA TCACACATCTTGAACCTTCCAGGGATGGGTCCTTGCTGCTGACATCTGCTACTTGGAGCC AGCCTTTGTCTGCACTTTGGGGAATGAAGTCAGTATTTGATATGAAGCATTCCTTCACAG AAGATCACTATGTTGAGTTCAGTAAGCACTCCCAGGATCGGGTCATCGGCACAAAAGGAG ACATTGCCCACATTTATGATATTCAGACTGGCAACAAGCTGTTGACTCTGTTTAACCCAG ATCTTGCCAACAACTACAAGAGGAACTGTGCCACCTTTAATCCTACAGATGATCTTGTCT TAAATGATGGCGTCCTCTGGGATGTCCGCTCTGCACAGGCCATCCACAAGTTTGACAAGT TCAATATGAACATCAGTGGTGTTTTCCATCCAAATGGACTGGAGGTGATCATTAATACTG AGATTTGGGACCTTCGAACTTTTCATCTTTTGCATACTGTTCCCGCTCTGGATCAGTGTC GCGTGGTGTTCAATCACACGGGAACAGTGATGTATGGAGCTATGTTGCAGGCAGATGATG AAGATGACTTAATGGAAGAGAGGATGAAAAGCCCCTTTGGGTCATCCTTCCGAACATTTA ATGCAACTGACTACAAACCTATAGCAACCATTGATGTGAAACGGAACATCTTTGACCTGT GTACAGACACCAAAGACTGCTATCTTGCTGTCATTGAGAATCAAGGCAGCATGGATGCCC TGAACATGGACACAGTATGCAGGCTGTATGAAGTGGGCAGGCAGCGTCTGGCAGAGGATG AGGATGAAGAGGAGGACCAGGAAGAGGAAGAACAGGAGGAAGAAGATGATGATGAAGATG ATGATGACACCGATGATTTAGATGAGCTTGACACTGACCAGTTGCTGGAGGCGGAGTTGG AGGAGGACGACAATAATGAGAACGCAGGGGAAGATGGGGACAATGACTTCTCTCCCTCTG ATGAGGAGCTAGCAAACCTTCTAGAGGAGGGAGAGGACGGGGAGGATGAAGACTCTGATG CAGATGAGGAGGTGGAACTGATCCTGGGGGACACTGACAGCTCTGACAACTCTGATTTGG AAGATGACATCATCTTATCTCTGAATGAGTGAGGAGCCATCACTGCTTGGAAGAGATTCT TGGCAGAGAGAAGAGGGGACAAATTTGGTTGTGAAGTAAACAAGAAACACAAGACGGCTA AAACTCTCCAGGGGCAGGTAAAGCCCTGCTGGGCTCAGAAGCACTATAGAATTTGCTGTA CACACTGAAGTGGGTCTGTGTCACACAGGTATTTCTTGGTCCTCTGGAGAGCAGAGGACT CACTCAGCTCTTTGCTTCATGCCTCGCTTTAATGGTAGTAAACACTTAACCAGTGGAATA GTCTGACTGCTGGTACTGATGGCATCAGAAGAAAACAGGTTAACAATCAAGTAGTGAAAG CACTATATTCTAGTCATTAACTCAACGGAGCCAGTCTCAACTCTGCGGGGAGCACCCACA GGTCTCTGTGGGGGAGACAGGGACTATAACAGGGAGGCAGAATTCAGGTTGGCTTGACAA CACACCTTAGGAACATGTGCTAAGGGGCTAAGGAGGCATCAGCTGTCTTCCTTATTCTAC CAGGGCTGAAAATCGGAGTCACTTAGGAGGCAGCATGAGAACAGTGGCACACCACCAGTG AGGCACATGTTGCCTTTTTGTCTTTAAGCAACACCTTAGACAGGGCCTGAACCCTGGGTG GGGGCACTGGATGCCTGAAGTAAGTAAGTTTTCTCAGTGGGGCAGTCTCATAAAATCACC AGGACATTGACCACCTGACCTCCCCTAACTAGTGCTGGCCAAGGCCTGAGAGTTCACAGA ACCTGCTAGGTCTTAAGAGCCAAAGGAAGGAAGTGGTTGAGGGCCAGGAAAGAGAAGGAA TGTCATGCCAGGGTGTTACTCCTCACCAAAACTTGGCAGTTCTTTTTGGGGCCATGGGAC ACCTTTCTCTGAAGAGGGGTTGGATAAGGCAAAGGTTGAAGTGTCTTCTCTAGGCACGTT TTCTAGCATGTTTCCTTTTACTTGGTTCTGTCTCACAAAGCTGGGAGGTAGATGATTCTT CCGGGTCCTGAGCACCTGCTTCATGAAGGAAGATAGGTAGCCCAGAGAAGAAGTTTTGGA CTTAACAACATACCAAGGCAGCTCTGGCCTGTGACTCAGGCGTTTGTTGGAGAGCTGCAG GAAAACCACTGCAGTGTGCCTGCAACAGGACAAGACCCACTCCTGTTTCTGGCGGTACCA GCTACCCTGGAATGGTGGCGTGTCCTGCTGTACCCAACTGACTTGCTTAGGTACCTGGCA GCTGTGACCACAGCTCAAGTAAAAAAACAAAAACAGGCAAATAAATAAACATGCTTAACA TGAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_014703 |
| Insert Size | 5900 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain 3bp deletion compared with NM_014703.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014703.1, NP_055518.1 |
| RefSeq Size | 5984 bp |
| RefSeq ORF | 4524 bp |
| Locus ID | 9730 |
| UniProt ID | Q9Y4B6 |
| Domains | LisH |
| Gene Summary | Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1, 3, 4, and 5 all encode the same isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210325 | VPRBP (Myc-DDK-tagged)-Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 1 |
CNY 15,464.00 |
|
| RC210325L3 | Lenti-ORF clone of VPRBP (Myc-DDK-tagged)-Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 1 |
CNY 16,910.00 |
|
| RC210325L4 | Lenti-ORF clone of VPRBP (mGFP-tagged)-Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 1 |
CNY 16,910.00 |
|
| RG210325 | VPRBP (tGFP-tagged) - Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 1 |
CNY 16,530.00 |
Customer
Reviews
Loading...
