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DOLK (NM_014908) Human Untagged Clone
CAT＃: SC114752
DOLK (untagged)-Human dolichol kinase (DOLK)
CNY 4,016.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDG1M; DK; DK1; SEC59; TMEM15 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114752 sequence for NM_014908 edited (data generated by NextGen Sequencing)
ATGACCCGAGAGTGCCCATCTCCGGCCCCGGGGCCTGGGGCTCCGCTGAGTGGATCGGTG CTGGCAGAGGCGGCAGTAGTGTTTGCAGTGGTGCTGAGCATCCACGCAACCGTATGGGAC CGATACTCGTGGTGCGCCGTGGCCCTCGCAGTGCAGGCCTTCTACGTCCAATACAAGTGG GACCGGCTGCTACAGCAGGGAAGCGCCGTCTTCCAGTTCCGAATGTCCGCAAACAGTGGC CTATTGCCCGCCTCCATGGTCATGCCTTTGCTTGGACTAGTCATGAAGGAGCGGTGCCAG ACTGCTGGGAACCCGTTCTTTGAGCGTTTTGGCATTGTGGTGGCAGCCACTGGCATGGCA GTGGCCCTCTTCTCATCAGTGTTGGCGCTCGGCATCACTCGCCCAGTGCCAACCAACACT TGTGTCATCTTGGGCTTGGCTGGAGGTGTTATCATTTATATCATGAAGCACTCGTTGAGC GTGGGGGAGGTGATCGAAGTCCTGGAAGTCCTTCTGATCTTCGTTTATCTCAACATGATC CTGCTGTACCTGCTGCCCCGCTGCTTCACCCCTGGTGAGGCACTGCTGGTATTGGGTGGC ATTAGCTTTGTCCTCAACCAGCTCATCAAGCGCTCTCTGACACTGGTGGAAAGTCAGGGG GACCCAGTGGACTTCTTCCTGCTGGTGGTGGTAGTAGGGATGGTACTCATGGGCATTTTC TTCAGCACTCTGTTTGTCTTCATGGACTCAGGCACCTGGGCCTCCTCCATCTTCTTCCAC CTCATGACCTGTGTGCTGAGCCTTGGTGTGGTCCTACCCTGGCTGCACCGGCTCATCCGC AGGAATCCCCTGCTCTGGCTTCTTCAGTTTCTCTTCCAGACAGACACCCGCATCTACCTC CTAGCCTATTGGTCTCTGCTGGCCACCTTGGCCTGCCTGGTGGTGCTGTACCAGAATGCC AAGCGGTCATCTTCCGAGTCCAAGAAGCACCAGGCCCCCACCATCGCCCGAAAGTATTTC CACCTCATTGTGGTAGCCACCTACATCCCAGGTATCATCTTTGACCGGCCACTGCTCTAT GTAGCCGCCACTGTATGCCTGGCGGTCTTCATCTTCCTGGAGTATGTGCGCTACTTCCGC ATCAAGCCTTTGGGTCACACTCTACGGAGCTTCCTGTCCCTTTTTCTGGATGAACGAGAC AGTGGACCACTCATTCTGACACACATCTACCTGCTCCTGGGCATGTCTCTTCCCATCTGG CTGATCCCCAGACCCTGCACACAGAAGGGTAGCCTGGGAGGAGCCAGGGCCCTCGTCCCC TATGCCGGTGTCCTGGCTGTGGGTGTGGGTGATACTGTGGCCTCCATCTTCGGTAGCACC ATGGGGGAGATCCGCTGGCCTGGAACCAAAAAGACTTTTGAGGGGACCATGACATCTATA TTTGCGCAGATCATTTCTGTAGCTCTGATCTTAATCTTTGACAGTGGAGTGGACCTAAAC TACAGTTATGCTTGGATTTTGGGGTCCATCAGCACTGTGTCCCTCCTGGAAGCATACACT ACACAGATAGACAATCTCCTTCTGCCTCTCTACCTCCTGATATTGCTGATGGCCTAG Clone variation with respect to NM_014908.3 >OriGene 5' read for NM_014908 unedited
CGTCACGATTTGTATACGACTCACTATAGGCGGCCGCGNATTCGGCACGAGGCAGAGTGG CGACGTCTACGCCGTAGGTTGGAGGCTGTGGGGGGTGGCCGGGCGCCAGCTCCCAGGCCG CAGAAGTGACCTGCGGTGGAGTTCCCTCCTCGCTGCTGGAGAACGGAGGGAGAAGGTTGC TGGCCGGGTGAAAGTGCCTCCCTCTGCTTGACGGGGCTGAGGGGCCCGAAGTCTAGGGCG TCCGTAGTCGCCCCGGCCTCCGTGAAGCCCCAGGTCTAGAGATATGACCCCGAGAGTGCC CATCTCCGGCCCCGGGGCCTGGGGCTCCGCTGAGTGGATCGGTGCTGGCAGAGGCGGCAG TAGTGTTTGCAGTGGTGCTGAGCATCCACGCAACCGTATGGGACCGATACTCGTGGTGCG CCGTGGCCCTCGCAGTGCAGGCCTTCTACGTCCAATACAAGTGGGACCGGCTGCTACAGC AGGGAAGCGCCGTCTTCCAGTTCCGAATGTCCGCAAACAGTGGCCTATTGCCCGCCTCCA TGGTCATGCCTTTGCTTGGACTAGTCATGAAGGAGCGGTGCCAGACTGCTGGGAACCCGT TCTTTGAGCGTTTTGGCATTGTGGTGGCAGCCACTGGCATGGCAGTGGCCCTCTTCTCAT CAGTGTTGGCGCTCGGCATCACTCGCCCAGTGCCAACCAACACTTGTGTCATCTTGGGCT TGGCTGGAGGTGTTATCATTTATATCATGAAGCACTCGTTGAGCGTGGGGGAGGTGATCG AAGTCCTGGAAGTCCTTCTGATCTTCGNTTATCTCACATGATCCTGCTGTACCTGCTGCC CCGCTGCTTCACCCCTGGTGAGGCACTGCTGGTATTGGGTGGCATTAGCTTTGTCCTCAA CCCGCT >OriGene 3' read for NM_014908 unedited
CCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTAGGAGAGCTTTGCTT TTTATTTTAAATCTGAATCAACTGAAAAATCAAATCTGCTTTTCACACCTTGGCTCTTCA TGCCCAAGTAGCTGTCTGCTGTGGGGACTGTTCACCCTCCCCATGTCTGTTTCCTCCGTC ACTGCTGCTGCACTGTAACAGCTAGGCCATCAGCAATATCAGGAGGTAGAGAGGCAGAAG GAGATTGTCTATCTGTGTAGTGTATGCTTCCAGGAGGGACACAGTGCTGATGGACCCCAA AATCCAAGCATAACTGTAGTTTAGGTCCACTCCACTGTCAAAGATTAAGATCAGAGCTAC AGAAATGATCTGCGCAAATATAGATGTCATGGTCCCCTCAAAAGTCTTTTTGGTTCCAGG CCAGCGGATCTCCCCCATGGTGCTACCGAAGATGGAGGCCACAGTATCACCCACACCCAC AGCCAGGACACCGGCATAGGGGACGAGGGCCCTGGCTCCTCCCAGGCTACCCTTCTGTGT GCAGGGTCTGGGGATCAGCCAGATGGGAAGAGACATGCCCAGGAGCAGGTAGATGTGTGT CAGAATGAGTGGTCCACTGTCTCGTTCATCCAGAAAAAGGGACAGGAAGCTCCGTAAAGT GTGACCCANAGGCTTGATGCGNAAGTAGCGCACATACTCCAGGAAGATGAAGACCGNCAG GCATACAGTGGCGGCTACATAGAGCCATGGCCGGTCAAAGATGATACCTGGGATGTAAGT GGCTACCACCATGANGTGGGAAATACTTTCGGCCGATGTTGGGGGCCTGNGGCCTTCTGG ACTCCGAAGTGACCGCTTGGCATTCTGTACAGCACCACCAAGCAGCCCAAGTGGCCACCN AGACAATTAGGTAGGAGGAAAAGCCGTTGTTCGTCTGGAAAAAACTGATACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014908 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014908.3, NP_055723.1 |
| RefSeq Size | 2267 bp |
| RefSeq ORF | 1617 bp |
| Locus ID | 22845 |
| UniProt ID | Q9UPQ8 |
| Protein Families | Transmembrane |
| Gene Summary | The protein encoded by this gene catalyzes the CTP-mediated phosphorylation of dolichol, and is involved in the synthesis of Dol-P-Man, which is an essential glycosyl carrier lipid for C- and O-mannosylation, N- and O-linked glycosylation of proteins, and for the biosynthesis of glycosyl phosphatidylinositol anchors in endoplasmic reticulum. Mutations in this gene are associated with dolichol kinase deficiency.[provided by RefSeq, Apr 2010] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207095 | DOLK (Myc-DDK-tagged)-Human dolichol kinase (DOLK) |
CNY 4,008.00 |
|
| RC207095L3 | Lenti ORF clone of Human dolichol kinase (DOLK), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207095L4 | Lenti ORF clone of Human dolichol kinase (DOLK), mGFP tagged |
CNY 5,890.00 |
|
| RG207095 | DOLK (tGFP-tagged) - Human dolichol kinase (DOLK) |
CNY 5,608.00 |
|
| SC324302 | DOLK (untagged)-Human dolichol kinase (DOLK) |
CNY 4,016.00 |
