Josephin 1 (JOSD1) (NM_014876) Human Untagged Clone

CAT#: SC114727

JOSD1 (untagged)-Human Josephin domain containing 1 (JOSD1)



  "NM_014876" in other vectors (7)

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
JOSD1 mouse monoclonal antibody, clone OTI3B11 (formerly 3B11)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms dJ508I15.2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC114727 sequence for NM_014876 edited (data generated by NextGen Sequencing)
ATGAGTTGTGTGCCATGGAAAGGAGACAAGGCCAAATCTGAATCATTGGAGCTGCCCCAG
GCAGCACCCCCACAAATCTACCATGAGAAACAGCGCAGGGAGCTTTGTGCCCTCCACGCC
CTCAATAACGTCTTCCAGGACAGCAATGCCTTCACCCGGGATACGCTGCAAGAGATTTTC
CAGAGGTTGTCTCCAAACACCATGGTGACACCTCACAAGAAGAGCATGCTGGGAAATGGC
AACTACGATGTGAATGTCATTATGGCAGCACTTCAGACCAAAGGCTATGAAGCTGTTTGG
TGGGACAAGCGCAGGGATGTCGGTGTCATTGCCCTCACTAACGTCATGGGCTTCATCATG
AATCTGCCCTCCAGCCTATGCTGGGGTCCACTGAAACTGCCCCTCAAAAGGCAGCACTGG
ATCTGTGTTCGAGAGGTGGGAGGGGCCTACTACAACCTCGACTCCAAACTCAAGATGCCC
GAGTGGATTGGAGGCGAGAGCGAGCTCAGGAAGTTTCTAAAACATCATTTGCGAGGAAAG
AACTGTGAACTCCTGCTGGTGGTACCAGAAGAGGTAGAGGCTCATCAGAGTTGGAGGACC
GATGTGTAA

Clone variation with respect to NM_014876.5
>OriGene 5' read for NM_014876 unedited
GGGTTCAGAAATTATGTATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGCTT
ACATTTTATGTGTTTTTTTAAATTCTGCACTTTGACAGTGGTAGCAAAATAAAAGTAATC
CAAAAGTTATTTTTCTATTGAGAAGAGAGAAGAGGTGGGTTTTATTTTTTTCCTTTTGAT
TCCAATCTTTTCTCCCAGTGACAAAGCATAAATCATGGACACCAGAGAATAAGGTGGACC
TCAGGAATCCGGAAAAGACTGAGAAGCTTACATTCTTCCTCTAGAGGGAAGAGTGGGGTG
TTTATAGCCATCTCTGGAACCTAAAACAAAAAACATGAGTTGTGTGCCATGGAAAGGAGA
CAAGGCCAAATCTGAATCATTGGAGCTGCCCCAGGCAGCACCCCCACAAATCTACCATGA
GAAACAGCGCAGGGAGCTTTGTGCCCTCCACGCCCTCAATAACGTCTTCCAGGACAGCAA
TGCCTTCACCCGGGATACGCTGCAAGAGATTTTCCAGAGGTTGTCTCCAAACACCATGGT
GACACCTCACAAGAAGAGCATGCTGGGAAATGGCAACTACGATGTGAATGTCATTATGGC
AGCACTTCAGACCAAAGGCTATGAAGCTGTTTGGTGGGACAAGCGCAGGGATGTCGGTGT
CATTGCCCTCACTAACGTCATGGGCTTCATCATGAATCTGCCCTCCAGCCTATGCTGGGG
TTCACTGGAACTGCCCCTCAAAAGCAGCACTGGATCTGTTGTCGAGAGGTGGGGAGGGCC
TACTACACCTCGACTCCAACTCAAGTGCCCGATGGATTGGAGCGAGAGCACCTCAGAAGT
CTAAACATATTGCGAGGAAGACTGTGACTCTGCTGTGGACCAGAGAGTANAGCTATCACA
GTTGAGACCGTGGTACAGTCTG
>OriGene 3' read for NM_014876 unedited
GGCTCCGGGCCACCGTCGAGTATCGAGTTAATCCTTTTTTTTTTTGGAGTTTTCCATTTT
TAATATTTGGTTAAACAAAATACATCTTTTGTAAACAAACGCAGCACAAGGCCAACAACA
AAAAGGAATAAAAGCAAGAAAAACAAAAAGCCTAAGGTTGCCCGTTATTGGGCATCAGGG
TCAGGCCTGTTCACTTCCTTAAAGGAAGTGGGGCTTTTTGCCCTTGGGCATCAGCATGGG
TCCCTGTTCCTAAGCACCAGAATTGGATATGAACAGAGAAACCAGCCCTGAAATGTTTAA
GCGTCTTTGCATATCCCATTGGCTCATGGACTCCAAGTGCATGGGGACTCTTTCAAGTGC
CATTTGCCCAGGCCCAAGTGGATTTGTGGAGAGGAGTTTCCTCCTCTACCCAAGAAATGG
CTTACTATCTGACCGTGCCAGTGGCCCCATGGTCCTGCTTGTGATCTTCAGCTAGGGTGG
CTGCTTCGCCTGGCTGGCGGCGGTCGGATTGTCCTTGTGCTGGGCTCGATAAGAGCTCCC
TGTGGCGCCCTGCTGGCCTGGTTGTTTTTTTGGTGCCTCTTATACTGCCGCCTTTCGCTT
GTCTTCTTTTCCGCCTCCTCCCTCTGGGGCCTGTTCCTCCTTTCTTCCCTCCCGCCTTGT
GGGCCTGGTGCCCCCGCTGCCTGTTTTGGCCTGGGCTTGCCCGTCTTTCTTCTCTTTGTT
GCTCTTTTTCTTTCGCTCCTCGGCTTTCTCTGCTTCTCTCTTTTCTTTTCTCCTTCTTCT
CTCTTCTCTTTCTCTCCCCTCCTCTCTCTCTCTTCTCCTGTTCTCGTCTTGGCCTTTTTT
CCTCTCCTCTTCTGCCCTCTTCGGCTCCTCTCTCGTTTCCGCTTTCCCCTCCTCCCCCGT
CCTCCGCCTTCTCGCTTCCCTCTCCCCATCGCTCTCTGCTTCGCCCCCCTCTTTCTCGTC
NTCTCTTCTCTCGTTCCCCCTCCCCTCTTGGCTCCCCGCGTTCGCTACGCACTACAACAC
AAACACAAACAAGAAC
Restriction Sites NotI-NotI     
ACCN NM_014876
Insert Size 3240 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014876.3, NP_055691.1
RefSeq Size 3207 bp
RefSeq ORF 609 bp
Locus ID 9929
UniProt ID Q15040
Domains Josephin
Protein Families Druggable Genome
Gene Summary Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, and 3 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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