Josephin 1 (JOSD1) (NM_014876) Human Untagged Clone
CAT#: SC114727
JOSD1 (untagged)-Human Josephin domain containing 1 (JOSD1)
CNY 3,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | dJ508I15.2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC114727 sequence for NM_014876 edited (data generated by NextGen Sequencing)
ATGAGTTGTGTGCCATGGAAAGGAGACAAGGCCAAATCTGAATCATTGGAGCTGCCCCAG GCAGCACCCCCACAAATCTACCATGAGAAACAGCGCAGGGAGCTTTGTGCCCTCCACGCC CTCAATAACGTCTTCCAGGACAGCAATGCCTTCACCCGGGATACGCTGCAAGAGATTTTC CAGAGGTTGTCTCCAAACACCATGGTGACACCTCACAAGAAGAGCATGCTGGGAAATGGC AACTACGATGTGAATGTCATTATGGCAGCACTTCAGACCAAAGGCTATGAAGCTGTTTGG TGGGACAAGCGCAGGGATGTCGGTGTCATTGCCCTCACTAACGTCATGGGCTTCATCATG AATCTGCCCTCCAGCCTATGCTGGGGTCCACTGAAACTGCCCCTCAAAAGGCAGCACTGG ATCTGTGTTCGAGAGGTGGGAGGGGCCTACTACAACCTCGACTCCAAACTCAAGATGCCC GAGTGGATTGGAGGCGAGAGCGAGCTCAGGAAGTTTCTAAAACATCATTTGCGAGGAAAG AACTGTGAACTCCTGCTGGTGGTACCAGAAGAGGTAGAGGCTCATCAGAGTTGGAGGACC GATGTGTAA Clone variation with respect to NM_014876.5 >OriGene 5' read for NM_014876 unedited
GGGTTCAGAAATTATGTATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGCTT ACATTTTATGTGTTTTTTTAAATTCTGCACTTTGACAGTGGTAGCAAAATAAAAGTAATC CAAAAGTTATTTTTCTATTGAGAAGAGAGAAGAGGTGGGTTTTATTTTTTTCCTTTTGAT TCCAATCTTTTCTCCCAGTGACAAAGCATAAATCATGGACACCAGAGAATAAGGTGGACC TCAGGAATCCGGAAAAGACTGAGAAGCTTACATTCTTCCTCTAGAGGGAAGAGTGGGGTG TTTATAGCCATCTCTGGAACCTAAAACAAAAAACATGAGTTGTGTGCCATGGAAAGGAGA CAAGGCCAAATCTGAATCATTGGAGCTGCCCCAGGCAGCACCCCCACAAATCTACCATGA GAAACAGCGCAGGGAGCTTTGTGCCCTCCACGCCCTCAATAACGTCTTCCAGGACAGCAA TGCCTTCACCCGGGATACGCTGCAAGAGATTTTCCAGAGGTTGTCTCCAAACACCATGGT GACACCTCACAAGAAGAGCATGCTGGGAAATGGCAACTACGATGTGAATGTCATTATGGC AGCACTTCAGACCAAAGGCTATGAAGCTGTTTGGTGGGACAAGCGCAGGGATGTCGGTGT CATTGCCCTCACTAACGTCATGGGCTTCATCATGAATCTGCCCTCCAGCCTATGCTGGGG TTCACTGGAACTGCCCCTCAAAAGCAGCACTGGATCTGTTGTCGAGAGGTGGGGAGGGCC TACTACACCTCGACTCCAACTCAAGTGCCCGATGGATTGGAGCGAGAGCACCTCAGAAGT CTAAACATATTGCGAGGAAGACTGTGACTCTGCTGTGGACCAGAGAGTANAGCTATCACA GTTGAGACCGTGGTACAGTCTG >OriGene 3' read for NM_014876 unedited
GGCTCCGGGCCACCGTCGAGTATCGAGTTAATCCTTTTTTTTTTTGGAGTTTTCCATTTT TAATATTTGGTTAAACAAAATACATCTTTTGTAAACAAACGCAGCACAAGGCCAACAACA AAAAGGAATAAAAGCAAGAAAAACAAAAAGCCTAAGGTTGCCCGTTATTGGGCATCAGGG TCAGGCCTGTTCACTTCCTTAAAGGAAGTGGGGCTTTTTGCCCTTGGGCATCAGCATGGG TCCCTGTTCCTAAGCACCAGAATTGGATATGAACAGAGAAACCAGCCCTGAAATGTTTAA GCGTCTTTGCATATCCCATTGGCTCATGGACTCCAAGTGCATGGGGACTCTTTCAAGTGC CATTTGCCCAGGCCCAAGTGGATTTGTGGAGAGGAGTTTCCTCCTCTACCCAAGAAATGG CTTACTATCTGACCGTGCCAGTGGCCCCATGGTCCTGCTTGTGATCTTCAGCTAGGGTGG CTGCTTCGCCTGGCTGGCGGCGGTCGGATTGTCCTTGTGCTGGGCTCGATAAGAGCTCCC TGTGGCGCCCTGCTGGCCTGGTTGTTTTTTTGGTGCCTCTTATACTGCCGCCTTTCGCTT GTCTTCTTTTCCGCCTCCTCCCTCTGGGGCCTGTTCCTCCTTTCTTCCCTCCCGCCTTGT GGGCCTGGTGCCCCCGCTGCCTGTTTTGGCCTGGGCTTGCCCGTCTTTCTTCTCTTTGTT GCTCTTTTTCTTTCGCTCCTCGGCTTTCTCTGCTTCTCTCTTTTCTTTTCTCCTTCTTCT CTCTTCTCTTTCTCTCCCCTCCTCTCTCTCTCTTCTCCTGTTCTCGTCTTGGCCTTTTTT CCTCTCCTCTTCTGCCCTCTTCGGCTCCTCTCTCGTTTCCGCTTTCCCCTCCTCCCCCGT CCTCCGCCTTCTCGCTTCCCTCTCCCCATCGCTCTCTGCTTCGCCCCCCTCTTTCTCGTC NTCTCTTCTCTCGTTCCCCCTCCCCTCTTGGCTCCCCGCGTTCGCTACGCACTACAACAC AAACACAAACAAGAAC |
Restriction Sites | NotI-NotI |
ACCN | NM_014876 |
Insert Size | 3240 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_014876.3, NP_055691.1 |
RefSeq Size | 3207 bp |
RefSeq ORF | 609 bp |
Locus ID | 9929 |
UniProt ID | Q15040 |
Domains | Josephin |
Protein Families | Druggable Genome |
Gene Summary | Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, and 3 encode the same protein. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201968 | JOSD1 (Myc-DDK-tagged)-Human Josephin domain containing 1 (JOSD1) |
CNY 3,600.00 |
|
RC201968L1 | Lenti ORF clone of Human Josephin domain containing 1 (JOSD1), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC201968L2 | Lenti ORF clone of Human Josephin domain containing 1 (JOSD1), mGFP tagged |
CNY 5,890.00 |
|
RC201968L3 | Lenti ORF clone of Human Josephin domain containing 1 (JOSD1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201968L4 | Lenti ORF clone of Human Josephin domain containing 1 (JOSD1), mGFP tagged |
CNY 5,890.00 |
|
RG201968 | JOSD1 (tGFP-tagged) - Human Josephin domain containing 1 (JOSD1) |
CNY 5,200.00 |
|
SC322360 | JOSD1 (untagged)-Human Josephin domain containing 1 (JOSD1) |
CNY 3,600.00 |
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