MRPS16 (NM_016065) Human Untagged Clone
CAT#: SC114526
MRPS16 (untagged)-Human mitochondrial ribosomal protein S16 (MRPS16), nuclear gene encoding mitochondrial protein
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CGI-132; COXPD2; MRP-S16; RPMS16 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_016065, the custom clone sequence may differ by one or more nucleotides
ATGGTCCACCTCACTACTCTCCTCTGCAAGGCCTACCGTGGGGGCCACTTAACCATCCGCCTTGCCCTGG GTGGCTGCACCAATCGGCCGTTCTACCGCATTGTGGCTGCTCACAACAAGTGTCCCAGGGATGGCCGTTT CGTAGAGCAGCTGGGCTCCTATGATCCATTGCCCAACAGTCATGGAGAAAAACTCGTTGCCCTCAACCTA GACAGGATCCGTCATTGGATTGGCTGCGGGGCCCACCTCTCTAAGCCTATGGAAAAGCTTCTGGGTCTTG CTGGCTTTTTCCCTCTGCATCCTATGATGATCACAAATGCTGAGAGACTGCGAAGGAAACGGGCACGTGA AGTCCTGTTAGCTTCTCAGAAAACAGATGCAGAAGCTACAGATACAGAGGCTACAGAAACATAA >OriGene 5' read for NM_016065 unedited
NGTTCACCATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCGAGGTTT TCGGGCTTGTACCGCTTGGCGGTGCGGCCTGGTGTCGGCTTGCAGGTTCTTTCTGTGTTT GTTCTCTGCCCTGCCAAGGCCGTAGAGCTGGTGCGTGCGGGTAGCGGGGCTCTCCGAGGA GCCGCACGCCGGCGGCACCATGGTCCACCTCACTACTCTCCTCTGCAAGGCCTACCGTGG GGGCCACTTAACCATCCGCCTTGCCCTGGGTGGCTGCACCAATCGGCCGTTCTACCGCAT TGTGGCTGCTCACAACAAGTGTCCCAGGGATGGCCGTTTCGTAGAGCAGCTGGGCTCCTA TGATCCATTGCCCAACAGTCATGGAGAAAAACTCGTTGCCCTCAACCTAGACAGGATCCG TCATTGGATTGGCTGCGGGGCCCACCTCTCTAAGCCTATGGAAAAGCTTCTGGGTCTTGC TGGCTTTTTCCCTCTGCATCCTATGATGATCACAAATGCTGAGAGACTGCGAAGGAAACG GGCACGTGAAGTCCTGTTAGCTTCTCAGAAAACAGATGCAGAAGCTACAGATACAGAGGC TACAGAAACATAAATGAGCTGACTTTAGTGAGCATAGCAGTGGGAACAAGGTCAAGGTCC TTTTGAAACACTGCAGCGATCTTAATTTTGTTAGATTTGGAGTTCAATAAATGGAGTATC CTGAGTTGCCCTTGCTCTTCTGGGCCTGCCTGCACAGGGCCCACGGAGAGATTGTTCTTG TGTGACTTAAAGCTGGGTGTGGGTACTAATTAGCTTTTTTCGACTTTGTCTTGGGGTAAA CAGTGGCTAGGGGAGGATGGGACTTTTGAGTGGGCCTCTGGGCCTCTTGGACACCTTCCA ATTAC >OriGene 3' read for NM_016065 unedited
CTTGAAAATATCCCCTTTTGATTGCGAACTGCAAATTTTCAAAAACCAGNAAAAGCAAAT TATTATGCTCTAGCCAAATTATGTAATGTTTTCTTTTTCTTTTCTCTGTCACCCAGGCTG GACTGTAGTGGAACAATCACAGTTCACTGCAGCATCGGCCTCCTGGGCTCAAGTGATCTT ACTGCCTCAGCTTCCCGAGTAGCTGGAACTATGGGTGAGCAACACCACAGCCAGCTAATT TTTTAAGTTTTTGTAGAGACGGGGTCTTGCTGTGTTGCACAGGCTGGTCTCAAACTCTGG CTTCAAGTGATCCTTCCACCTCAGCCTCCCAAAGTGCTGAGATTACAGGTGTGAGCCACT GCCCCAATGGTATCTTTATATTATTTTTCCTTACAAGTTACTTCAAATTCTTGATGTTGA ATTTCATCTCACTGACTTCAGGCTTTTAAAACACATGGGAATACTTGAGACCTAAGAATG GTTGTGAGTCAAATAAAAAAGAAGAACTAAAGTATACTGAAGTTATTCAAATACATTCAA ACTATAAAGATCCCCTTATAGATTACTGGCATCAAGGTAGGAAGGAAAAGATACAAGAAG TAAAATGCACCACTCATTACAGGTTGTCAACATTATTGGTATACAGTTTATCCTAACACA GAGCAGCCTTCTTAACCTGCTCCATAAATTACCACCAAGAAAGAAAGTCCAAGATAACGT CACAGCTGAATTGGCTTGGTGTCTTAATTCCTATTCAGTATTCTCAGAGGATCCCATCTT GAATATGCAAAATCACAGCCCATTTGAATGGTTCAATCTGATTATACTGAACTCCTTAAG ACCACCAGCTCCCCATCTTAAAAGGGGGCTATTAAGCCCCAACCAATTTCACACAAACCA CCCTGATATTTAAATTCCCTCACGCCTGGAGGGCGGCCCCCCCACAACCCACCTTTGGAG GCCAGGGGCATATCCAGCCAATGGACCCCCTGCCACGGGGAACCCCCC |
Restriction Sites | NotI-NotI |
ACCN | NM_016065 |
Insert Size | 2930 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_016065.3, NP_057149.1 |
RefSeq Size | 2651 bp |
RefSeq ORF | 414 bp |
Locus ID | 51021 |
UniProt ID | Q9Y3D3 |
Domains | Ribosomal_S16 |
Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205837 | MRPS16 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S16 (MRPS16), nuclear gene encoding mitochondrial protein |
CNY 1,200.00 |
|
RC205837L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S16 (MRPS16), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205837L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S16 (MRPS16), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RG205837 | MRPS16 (tGFP-tagged) - Human mitochondrial ribosomal protein S16 (MRPS16), nuclear gene encoding mitochondrial protein |
CNY 2,800.00 |